Click here to switch to the map view.

The map label for this gene is kbl [C]

Identifier: 150397425

GI number: 150397425

Start: 2303293

End: 2304480

Strand: Reverse

Name: kbl [C]

Synonym: Smed_2225

Alternate gene names: 150397425

Gene position: 2304480-2303293 (Counterclockwise)

Preceding gene: 150397426

Following gene: 150397424

Centisome position: 60.93

GC content: 62.12

Gene sequence:

>1188_bases
ATGACCAAGGCCTTCCTCTCCCATCTCAGCTCGGAACTTGAAGGCCTGAAGCAGGCCGGTCTCTACAAATCCGAGCGTGT
CATCACCTCAAAGCAGTCGGGCGAAATCGAAGTCGCTTCCGGTCAGCGTGTCCTGAATTTCTGTGCCAACAACTATCTCG
GGCTTGCCGGCAACGAGGAGCTTGCAGACGCGGCGAAGAAGGCCCTCGACCGTTATGGCTACGGCATGGCGTCGGTGCGC
TTCATCTGCGGCACGCAGGAGGAGCACAAGGAGCTCGAAGCGCGCATATCCTCCTTCCTCGGCATGGAGGACACGATTCT
CTATTCCTCCTGCTTCGATGCCAATGGCGGTCTGTTCGAGACGCTGCTCGGCGAGGACGACGCGATCATCTCCGATGCGC
TGAACCATGCTTCGATCATCGATGGCGTGCGTCTCTCCAAGGCCAAGCGCTTCCGCTATGCCAATAACGACATGGCGGCG
CTGGAAGAAGAGCTGAAGAAGGCGGAAGGCAGCCGCTTCAAGATGATCGCCACCGACGGCGTCTTCTCGATGGACGGCAT
CATCGCCAATCTCGCGGGCGTCTGCGATCTCGCCGAGAAATACGGTGCGATGGTCATGGTGGACGACAGCCATGCGGTCG
GTTTCGTCGGCAGGCATGGGCGCGGCTCTGCCGAGCATTGCGGGGTGGAGGGCCGGGTCGACATCATTACCGGCACGCTC
GGCAAGGCGCTTGGCGGCGCTTCGGGCGGGTATACGTCGGCAAAGGCCGAGCTCGTCGAATGGCTGCGCCAGCGCTCGCG
GCCCTATCTCTTCTCCAACACGCTCGCGCCCGTCATCGCCGCCGCTTCCCTCAAGGTCTTCGAGCTGATCGAAAACGGGG
ATGCGCTGCGCGAACGGCTTTCCGCAAACGCGGCGCTTTTCCGGGCAGAGATGTCCAGGCTCGGCTTCACGCTCGCCGGC
GAGGGCCATCCGATTATTCCTGTCATGCTCGGGGATGCGGCGCTCGCGCAGGAGATGGCCGCACGCATGCTTGAGAAGGG
CGTATACGTCATCGGCTTCTCCTTCCCGGTCGTGCCGAAAGGACAGGCGCGCATTCGCACGCAGATGTCCGCCGCGCACA
GCGAAGCGGATATCCGCCGCGCCATAGCCGTCTTCGAGGAGGTGGGCCGAGAGTTGAACGTGATTTAA

Upstream 100 bases:

>100_bases
TGAGGGGCATCAGCACGTGGCGTCGGAAACAGATTCCATGAAAAGTGCCACCGTGTTAGTCCTCTAGCTGGTGATTTCCA
TCGATTGCGAGCACTCAGCC

Downstream 100 bases:

>100_bases
GGGGCGAACGGGGGTCCCGCGCAACGCGGAGGAGCGAGCAATGACCAATATGATGAGGGCACTCGTAAAGACCAGGCCCG
AGGTGGGGCTCTGGATGGAG

Product: 2-amino-3-ketobutyrate coenzyme A ligase

Products: NA

Alternate protein names: AONS/AKB ligase; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; Alpha-oxoamine synthase; L-alanine--pimeloyl-CoA ligase [H]

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MTKAFLSHLSSELEGLKQAGLYKSERVITSKQSGEIEVASGQRVLNFCANNYLGLAGNEELADAAKKALDRYGYGMASVR
FICGTQEEHKELEARISSFLGMEDTILYSSCFDANGGLFETLLGEDDAIISDALNHASIIDGVRLSKAKRFRYANNDMAA
LEEELKKAEGSRFKMIATDGVFSMDGIIANLAGVCDLAEKYGAMVMVDDSHAVGFVGRHGRGSAEHCGVEGRVDIITGTL
GKALGGASGGYTSAKAELVEWLRQRSRPYLFSNTLAPVIAAASLKVFELIENGDALRERLSANAALFRAEMSRLGFTLAG
EGHPIIPVMLGDAALAQEMAARMLEKGVYVIGFSFPVVPKGQARIRTQMSAAHSEADIRRAIAVFEEVGRELNVI

Sequences:

>Translated_395_residues
MTKAFLSHLSSELEGLKQAGLYKSERVITSKQSGEIEVASGQRVLNFCANNYLGLAGNEELADAAKKALDRYGYGMASVR
FICGTQEEHKELEARISSFLGMEDTILYSSCFDANGGLFETLLGEDDAIISDALNHASIIDGVRLSKAKRFRYANNDMAA
LEEELKKAEGSRFKMIATDGVFSMDGIIANLAGVCDLAEKYGAMVMVDDSHAVGFVGRHGRGSAEHCGVEGRVDIITGTL
GKALGGASGGYTSAKAELVEWLRQRSRPYLFSNTLAPVIAAASLKVFELIENGDALRERLSANAALFRAEMSRLGFTLAG
EGHPIIPVMLGDAALAQEMAARMLEKGVYVIGFSFPVVPKGQARIRTQMSAAHSEADIRRAIAVFEEVGRELNVI
>Mature_394_residues
TKAFLSHLSSELEGLKQAGLYKSERVITSKQSGEIEVASGQRVLNFCANNYLGLAGNEELADAAKKALDRYGYGMASVRF
ICGTQEEHKELEARISSFLGMEDTILYSSCFDANGGLFETLLGEDDAIISDALNHASIIDGVRLSKAKRFRYANNDMAAL
EEELKKAEGSRFKMIATDGVFSMDGIIANLAGVCDLAEKYGAMVMVDDSHAVGFVGRHGRGSAEHCGVEGRVDIITGTLG
KALGGASGGYTSAKAELVEWLRQRSRPYLFSNTLAPVIAAASLKVFELIENGDALRERLSANAALFRAEMSRLGFTLAGE
GHPIIPVMLGDAALAQEMAARMLEKGVYVIGFSFPVVPKGQARIRTQMSAAHSEADIRRAIAVFEEVGRELNVI

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A and/or converts 2-amino-3-ketobutyrate to glycine and acetyl-CoA [H]

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI7657118, Length=384, Percent_Identity=55.9895833333333, Blast_Score=438, Evalue=1e-123,
Organism=Homo sapiens, GI284448556, Length=409, Percent_Identity=52.5672371638142, Blast_Score=429, Evalue=1e-120,
Organism=Homo sapiens, GI83977442, Length=368, Percent_Identity=35.054347826087, Blast_Score=211, Evalue=7e-55,
Organism=Homo sapiens, GI83977440, Length=368, Percent_Identity=35.054347826087, Blast_Score=211, Evalue=9e-55,
Organism=Homo sapiens, GI83977444, Length=368, Percent_Identity=35.054347826087, Blast_Score=211, Evalue=1e-54,
Organism=Homo sapiens, GI4502025, Length=378, Percent_Identity=31.7460317460317, Blast_Score=195, Evalue=5e-50,
Organism=Homo sapiens, GI40316939, Length=378, Percent_Identity=31.7460317460317, Blast_Score=195, Evalue=5e-50,
Organism=Homo sapiens, GI4758668, Length=369, Percent_Identity=33.6043360433604, Blast_Score=193, Evalue=2e-49,
Organism=Homo sapiens, GI119220554, Length=369, Percent_Identity=32.520325203252, Blast_Score=190, Evalue=2e-48,
Organism=Homo sapiens, GI5454084, Length=391, Percent_Identity=26.3427109974425, Blast_Score=116, Evalue=5e-26,
Organism=Escherichia coli, GI1790046, Length=397, Percent_Identity=68.2619647355164, Blast_Score=561, Evalue=1e-161,
Organism=Escherichia coli, GI1786993, Length=357, Percent_Identity=32.2128851540616, Blast_Score=190, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI71994529, Length=397, Percent_Identity=57.4307304785894, Blast_Score=464, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI32566772, Length=388, Percent_Identity=29.3814432989691, Blast_Score=178, Evalue=5e-45,
Organism=Caenorhabditis elegans, GI17560914, Length=364, Percent_Identity=30.2197802197802, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17560912, Length=364, Percent_Identity=30.2197802197802, Blast_Score=169, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI71982617, Length=276, Percent_Identity=26.4492753623188, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI71982625, Length=276, Percent_Identity=26.0869565217391, Blast_Score=108, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6320438, Length=411, Percent_Identity=32.6034063260341, Blast_Score=219, Evalue=4e-58,
Organism=Saccharomyces cerevisiae, GI6320267, Length=368, Percent_Identity=30.4347826086957, Blast_Score=180, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6323954, Length=275, Percent_Identity=29.0909090909091, Blast_Score=117, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24662918, Length=388, Percent_Identity=59.7938144329897, Blast_Score=483, Evalue=1e-136,
Organism=Drosophila melanogaster, GI17137420, Length=333, Percent_Identity=33.3333333333333, Blast_Score=196, Evalue=2e-50,
Organism=Drosophila melanogaster, GI17136286, Length=369, Percent_Identity=32.520325203252, Blast_Score=176, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24653280, Length=392, Percent_Identity=27.0408163265306, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24653276, Length=392, Percent_Identity=27.0408163265306, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24653278, Length=392, Percent_Identity=27.0408163265306, Blast_Score=123, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011282
- InterPro:   IPR001917
- InterPro:   IPR004839
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: =2.3.1.29; =2.3.1.47 [H]

Molecular weight: Translated: 42533; Mature: 42402

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKAFLSHLSSELEGLKQAGLYKSERVITSKQSGEIEVASGQRVLNFCANNYLGLAGNEE
CCHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCEEECCCHHHHHHHHCCCCCCCCCHH
LADAAKKALDRYGYGMASVRFICGTQEEHKELEARISSFLGMEDTILYSSCFDANGGLFE
HHHHHHHHHHHHCCCHHHEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHH
TLLGEDDAIISDALNHASIIDGVRLSKAKRFRYANNDMAALEEELKKAEGSRFKMIATDG
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEEECC
VFSMDGIIANLAGVCDLAEKYGAMVMVDDSHAVGFVGRHGRGSAEHCGVEGRVDIITGTL
CHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEEECCCCCCHHHCCCCCEEEEEECHH
GKALGGASGGYTSAKAELVEWLRQRSRPYLFSNTLAPVIAAASLKVFELIENGDALRERL
HHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
SANAALFRAEMSRLGFTLAGEGHPIIPVMLGDAALAQEMAARMLEKGVYVIGFSFPVVPK
HHHHHHHHHHHHHCCEEEECCCCCEEEEEECCHHHHHHHHHHHHHCCEEEEEEECCCCCC
GQARIRTQMSAAHSEADIRRAIAVFEEVGRELNVI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TKAFLSHLSSELEGLKQAGLYKSERVITSKQSGEIEVASGQRVLNFCANNYLGLAGNEE
CHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCEEECCCHHHHHHHHCCCCCCCCCHH
LADAAKKALDRYGYGMASVRFICGTQEEHKELEARISSFLGMEDTILYSSCFDANGGLFE
HHHHHHHHHHHHCCCHHHEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHH
TLLGEDDAIISDALNHASIIDGVRLSKAKRFRYANNDMAALEEELKKAEGSRFKMIATDG
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEEECC
VFSMDGIIANLAGVCDLAEKYGAMVMVDDSHAVGFVGRHGRGSAEHCGVEGRVDIITGTL
CHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEEECCCCCCHHHCCCCCEEEEEECHH
GKALGGASGGYTSAKAELVEWLRQRSRPYLFSNTLAPVIAAASLKVFELIENGDALRERL
HHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
SANAALFRAEMSRLGFTLAGEGHPIIPVMLGDAALAQEMAARMLEKGVYVIGFSFPVVPK
HHHHHHHHHHHHHCCEEEECCCCCEEEEEECCHHHHHHHHHHHHHCCEEEEEEECCCCCC
GQARIRTQMSAAHSEADIRRAIAVFEEVGRELNVI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]