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The map label for this gene is ubiF [H]

Identifier: 150396635

GI number: 150396635

Start: 1503409

End: 1504620

Strand: Direct

Name: ubiF [H]

Synonym: Smed_1421

Alternate gene names: 150396635

Gene position: 1503409-1504620 (Clockwise)

Preceding gene: 150396625

Following gene: 150396638

Centisome position: 39.75

GC content: 62.71

Gene sequence:

>1212_bases
ATGGATCACTATGACATTGCAATTATCGGCGCGGGACTTGCCGGTTCGCTGACGGCGATCGCCCTGGCCCGCAAGGGCCA
CCGGGTAGCGCTCGTCGGTCCGAAACCTGCGATAGCCGACGGACGAACCACCGCGCTCATGGACCAGTCGATCGGATATC
TGAAGACGCTCGGCCTTTGGGACGAGACCGAGCCATTGACGGCGCCGCTTGTCGCCATCCGCATCCTCGACGGGACCAGG
CGGCTCTTTCGGGCGCCCCCCGTCAACTTCCGCGCCTCGGAAGTGGGACTTGAGGCATTCGGCTATAACATACCGAATTC
GCCGTTCCTGGATATCCTGGAAAAGCATGAAATGGCGCTTTCGACGCTCGAGCGTACGACCGCCGCGGCAGCGAGATTCG
ATCTCGGCGGTGCGGAGGTTCTAATCGGCCTTGGCGATGGCCGCACGATCTCGGCCGATCTGGTAATCGGAGCCGATGGG
CGACGCTCGACCGTGCGGGAAGCCGCGGGGATCGATGTGAAAACCTGGTCCTATCCGCAGGCCGCTATCGTGCTGAATTT
CGGCCACGAACTGCCGCACCAGGACGTTTCGACCGAATTTCATACAGAAAGCGGTCCCTTCACTCAGGTCCCCCTTCCCG
GAAATCGCTCAAGTCTTGTCTGGGTTCAAAAGCCCCATGATGCAGAGGAGACGCTGAAGCTCGCGCCGGACGCGCTTTCG
CGGACCATAGAGGATCGCATGCAATCGATTCTCGGCAAGATCACCACCGAAGGAACGCCTCAGTCGTTTCCGCTTTCCGG
GCTTGCTGCCCGTCGTTTTGGCAAGGGGCGCATCCTCCTCGTCGGCGAAGCGGCCCATGCCTTTCCTCCGATCGGGGCAC
AAGGCCTGAATCTCAGCCTCCGAGACATCATGACCCTCGTCGATCTCATCGGCCGGCCGGCCGGAAGCCGCCTTCCGGCA
GACACGGGCGACCGCTTCGACGGTCGCCGCCGCGCCGACATCGTCAGCAGGACGGCAAGCGTCGACCTGCTGAACCGTTC
TCTTCTTTCGAATTTCCTGCCCGTGCAGGCCTTGCGTGCGGCGGGTCTGCATCTTCTCGCCTCTGCGGGCCCGCTGCGTG
GCCTTTTGATGCGTGAGGGGATTCACCCGGGCAGCGCCCTGCAAGCGTTCAAGGAGGGCTTACGGGAACAGATCGGCCGG
AAGAGCGCCTGA

Upstream 100 bases:

>100_bases
AAGAAGCCTCGCCATTACTGCAACCGGAAGGCTGCCATGATAGTTAAGTCATGGACGTCGAGACGAAACTGCGGGTAGCC
GCATCGGAGGTCCTGTCGAC

Downstream 100 bases:

>100_bases
GCGTATGAGATAAAGCAGGCCGGTTACCGTCGCGACCGAAAGCAGCGTCGTTATGAGAATTGTTGCCGATGCTCGTTCCT
GCCAGACGCCGTATTGGTGG

Product: 2-octaprenyl-6-methoxyphenyl hydroxylase

Products: 2-Octaprenyl-3-Methyl-5-Hydroxy-6-Methoxy-1,4- Benzoquinol [C]

Alternate protein names: NA

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MDHYDIAIIGAGLAGSLTAIALARKGHRVALVGPKPAIADGRTTALMDQSIGYLKTLGLWDETEPLTAPLVAIRILDGTR
RLFRAPPVNFRASEVGLEAFGYNIPNSPFLDILEKHEMALSTLERTTAAAARFDLGGAEVLIGLGDGRTISADLVIGADG
RRSTVREAAGIDVKTWSYPQAAIVLNFGHELPHQDVSTEFHTESGPFTQVPLPGNRSSLVWVQKPHDAEETLKLAPDALS
RTIEDRMQSILGKITTEGTPQSFPLSGLAARRFGKGRILLVGEAAHAFPPIGAQGLNLSLRDIMTLVDLIGRPAGSRLPA
DTGDRFDGRRRADIVSRTASVDLLNRSLLSNFLPVQALRAAGLHLLASAGPLRGLLMREGIHPGSALQAFKEGLREQIGR
KSA

Sequences:

>Translated_403_residues
MDHYDIAIIGAGLAGSLTAIALARKGHRVALVGPKPAIADGRTTALMDQSIGYLKTLGLWDETEPLTAPLVAIRILDGTR
RLFRAPPVNFRASEVGLEAFGYNIPNSPFLDILEKHEMALSTLERTTAAAARFDLGGAEVLIGLGDGRTISADLVIGADG
RRSTVREAAGIDVKTWSYPQAAIVLNFGHELPHQDVSTEFHTESGPFTQVPLPGNRSSLVWVQKPHDAEETLKLAPDALS
RTIEDRMQSILGKITTEGTPQSFPLSGLAARRFGKGRILLVGEAAHAFPPIGAQGLNLSLRDIMTLVDLIGRPAGSRLPA
DTGDRFDGRRRADIVSRTASVDLLNRSLLSNFLPVQALRAAGLHLLASAGPLRGLLMREGIHPGSALQAFKEGLREQIGR
KSA
>Mature_403_residues
MDHYDIAIIGAGLAGSLTAIALARKGHRVALVGPKPAIADGRTTALMDQSIGYLKTLGLWDETEPLTAPLVAIRILDGTR
RLFRAPPVNFRASEVGLEAFGYNIPNSPFLDILEKHEMALSTLERTTAAAARFDLGGAEVLIGLGDGRTISADLVIGADG
RRSTVREAAGIDVKTWSYPQAAIVLNFGHELPHQDVSTEFHTESGPFTQVPLPGNRSSLVWVQKPHDAEETLKLAPDALS
RTIEDRMQSILGKITTEGTPQSFPLSGLAARRFGKGRILLVGEAAHAFPPIGAQGLNLSLRDIMTLVDLIGRPAGSRLPA
DTGDRFDGRRRADIVSRTASVDLLNRSLLSNFLPVQALRAAGLHLLASAGPLRGLLMREGIHPGSALQAFKEGLREQIGR
KSA

Specific function: Oxygenase that introduces the hydroxyl group at carbon five of 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol resulting in the formation of 2-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4- benzoquinol [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Homo sapiens, GI32967305, Length=410, Percent_Identity=25.8536585365854, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI32967307, Length=334, Percent_Identity=26.9461077844311, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1786883, Length=388, Percent_Identity=30.6701030927835, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1789274, Length=391, Percent_Identity=27.1099744245524, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1789273, Length=398, Percent_Identity=29.1457286432161, Blast_Score=122, Evalue=5e-29,
Organism=Drosophila melanogaster, GI19920760, Length=439, Percent_Identity=27.7904328018223, Blast_Score=102, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 43116; Mature: 43116

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDHYDIAIIGAGLAGSLTAIALARKGHRVALVGPKPAIADGRTTALMDQSIGYLKTLGLW
CCCEEEEEEECCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHCCCHHHHCCCC
DETEPLTAPLVAIRILDGTRRLFRAPPVNFRASEVGLEAFGYNIPNSPFLDILEKHEMAL
CCCCCCHHCEEEEEEEHHHHHHHCCCCCCCCHHHHCHHHHCCCCCCCHHHHHHHHHHHHH
STLERTTAAAARFDLGGAEVLIGLGDGRTISADLVIGADGRRSTVREAAGIDVKTWSYPQ
HHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEEEEECCCCHHHHHHHCCCCEEECCCCC
AAIVLNFGHELPHQDVSTEFHTESGPFTQVPLPGNRSSLVWVQKPHDAEETLKLAPDALS
EEEEEECCCCCCCCCCCCCEECCCCCEEECCCCCCCCCEEEEECCCCHHHHHHHCHHHHH
RTIEDRMQSILGKITTEGTPQSFPLSGLAARRFGKGRILLVGEAAHAFPPIGAQGLNLSL
HHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCEEEEEECHHHCCCCCCCCCCCEEH
RDIMTLVDLIGRPAGSRLPADTGDRFDGRRRADIVSRTASVDLLNRSLLSNFLPVQALRA
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
AGLHLLASAGPLRGLLMREGIHPGSALQAFKEGLREQIGRKSA
CCHHEECCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDHYDIAIIGAGLAGSLTAIALARKGHRVALVGPKPAIADGRTTALMDQSIGYLKTLGLW
CCCEEEEEEECCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHCCCHHHHCCCC
DETEPLTAPLVAIRILDGTRRLFRAPPVNFRASEVGLEAFGYNIPNSPFLDILEKHEMAL
CCCCCCHHCEEEEEEEHHHHHHHCCCCCCCCHHHHCHHHHCCCCCCCHHHHHHHHHHHHH
STLERTTAAAARFDLGGAEVLIGLGDGRTISADLVIGADGRRSTVREAAGIDVKTWSYPQ
HHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEEEEECCCCHHHHHHHCCCCEEECCCCC
AAIVLNFGHELPHQDVSTEFHTESGPFTQVPLPGNRSSLVWVQKPHDAEETLKLAPDALS
EEEEEECCCCCCCCCCCCCEECCCCCEEECCCCCCCCCEEEEECCCCHHHHHHHCHHHHH
RTIEDRMQSILGKITTEGTPQSFPLSGLAARRFGKGRILLVGEAAHAFPPIGAQGLNLSL
HHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCEEEEEECHHHCCCCCCCCCCCEEH
RDIMTLVDLIGRPAGSRLPADTGDRFDGRRRADIVSRTASVDLLNRSLLSNFLPVQALRA
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
AGLHLLASAGPLRGLLMREGIHPGSALQAFKEGLREQIGRKSA
CCHHEECCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: @OCTA02.txt*2-Octaprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol! [C]

Specific reaction: @OCTA02.txt*2-Octaprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol! = 2-Octaprenyl-3-Methyl-5-Hydroxy-6-Methoxy-1,4- Benzoquinol [C]

General reaction: Hydroxylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10802164 [H]