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The map label for this gene is alaXM [H]

Identifier: 150396580

GI number: 150396580

Start: 1445358

End: 1446107

Strand: Direct

Name: alaXM [H]

Synonym: Smed_1364

Alternate gene names: 150396580

Gene position: 1445358-1446107 (Clockwise)

Preceding gene: 150396579

Following gene: 150396581

Centisome position: 38.22

GC content: 58.53

Gene sequence:

>750_bases
ATGACCAGATCTGTCACCGCCCTCTTCCGAGAAGATTTCTATCTTTCGACCTGCGAAGCAAGCGTGACGGCAATCCTGGA
AGACGGCGGCATCGAGCTCGATCAGACCTGCTTCTATGCGACCTCCGGCGGGCAACCGGGTGACAAGGGCTTTCTCGAGC
GCGAAGACGGGAGCCGCATAGACATCATCGATACGCGGCATGGTGCGACCAAGGACATCATCTTGCACATTCCCGCAGAA
GGAGCGCGCATGCCTGATGTCGGCGAAAAGCTGGTGCTGCACATAGACTGGCCGCGCCGCTACCGGCTGATGCGCATGCA
TACGGCTTGCCATCTTCTTTCAGTCGTCTGCCCTTTCCCGATCACCGGCGCAGCAGTCGGAGAAGAAGAAAGCCGGGTCG
ACTTCGACATGAGCGAATTGATCGACAAGGACCAGGTCACGGCAGCGCTGATGAAGCTCGTCCATGAGAACCATCCGGTC
TATGTGCAGTGGATCACCGACGAGGAGCTGGCCGCCAATCCGGCCATAGTCAAGTCTAAGAATGTCCGCCCGCCAATGGG
GCTTGGCCGCGTCAGTCTCGTCTGCATCGGCGAGAATTCATCGGTAGACAGCCAGCCGTGCGGCGGAACGCATGTTTCGG
AGACACAGGAAGTGGGCGCGATCCACATCGCGAAAATCGAAAAGAAAGGTAAAGAAAACCGGCGTTTCCGAATACGATTC
GCGGCGCCCGAGGATCGGGCCTTCGCCTGA

Upstream 100 bases:

>100_bases
AATCCGGACTTCCTGGCCTCCAAGGGTCTGCCTGTCCCGGCCTGGCTGAAGACGGCTTCGAACATTGCAGTGCCATACGA
ACCCGCTGGATAGGATTGCC

Downstream 100 bases:

>100_bases
GCGCTTTTTATGACCATGCCAGTGGAGTAGAACGATGAACGTTGAAAACGCTAACAGGAGCGACTTTGTCGTTTCTGCAG
ACTGGCTTGAGCAGCGCCTC

Product: threonyl/alanyl tRNA synthetase SAD

Products: AMP; diphosphate; L-alanyl-tRNAAla [C]

Alternate protein names: AlaX-M; Alanyl-tRNA deacylase AlaX-M [H]

Number of amino acids: Translated: 249; Mature: 248

Protein sequence:

>249_residues
MTRSVTALFREDFYLSTCEASVTAILEDGGIELDQTCFYATSGGQPGDKGFLEREDGSRIDIIDTRHGATKDIILHIPAE
GARMPDVGEKLVLHIDWPRRYRLMRMHTACHLLSVVCPFPITGAAVGEEESRVDFDMSELIDKDQVTAALMKLVHENHPV
YVQWITDEELAANPAIVKSKNVRPPMGLGRVSLVCIGENSSVDSQPCGGTHVSETQEVGAIHIAKIEKKGKENRRFRIRF
AAPEDRAFA

Sequences:

>Translated_249_residues
MTRSVTALFREDFYLSTCEASVTAILEDGGIELDQTCFYATSGGQPGDKGFLEREDGSRIDIIDTRHGATKDIILHIPAE
GARMPDVGEKLVLHIDWPRRYRLMRMHTACHLLSVVCPFPITGAAVGEEESRVDFDMSELIDKDQVTAALMKLVHENHPV
YVQWITDEELAANPAIVKSKNVRPPMGLGRVSLVCIGENSSVDSQPCGGTHVSETQEVGAIHIAKIEKKGKENRRFRIRF
AAPEDRAFA
>Mature_248_residues
TRSVTALFREDFYLSTCEASVTAILEDGGIELDQTCFYATSGGQPGDKGFLEREDGSRIDIIDTRHGATKDIILHIPAEG
ARMPDVGEKLVLHIDWPRRYRLMRMHTACHLLSVVCPFPITGAAVGEEESRVDFDMSELIDKDQVTAALMKLVHENHPVY
VQWITDEELAANPAIVKSKNVRPPMGLGRVSLVCIGENSSVDSQPCGGTHVSETQEVGAIHIAKIEKKGKENRRFRIRFA
APEDRAFA

Specific function: Functions in trans to edit the amino acid moiety from incorrectly charged Ser-tRNA(Ala) or Gly-tRNA(Ala). Has no activity on incorrectly charged Ser-tRNA(Thr), nor on correctly charged Ala-tRNA(Ala) or Ser-tRNA(Ser) [H]

COG id: COG2872

COG function: function code R; Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Editing domain AlaX-M subfamily [H]

Homologues:

Organism=Homo sapiens, GI109148542, Length=256, Percent_Identity=29.296875, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI13376886, Length=219, Percent_Identity=27.8538812785388, Blast_Score=65, Evalue=7e-11,
Organism=Homo sapiens, GI217416402, Length=219, Percent_Identity=27.8538812785388, Blast_Score=64, Evalue=9e-11,
Organism=Escherichia coli, GI1789048, Length=236, Percent_Identity=29.6610169491525, Blast_Score=68, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24582809, Length=243, Percent_Identity=29.2181069958848, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI45552267, Length=243, Percent_Identity=29.2181069958848, Blast_Score=68, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: 6.1.1.7 [C]

Molecular weight: Translated: 27589; Mature: 27458

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRSVTALFREDFYLSTCEASVTAILEDGGIELDQTCFYATSGGQPGDKGFLEREDGSRI
CCCCHHHHHHCCCEEEECCCEEEEEEECCCCEECCEEEEEECCCCCCCCCCEECCCCCEE
DIIDTRHGATKDIILHIPAEGARMPDVGEKLVLHIDWPRRYRLMRMHTACHLLSVVCPFP
EEEECCCCCCCEEEEEECCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCC
ITGAAVGEEESRVDFDMSELIDKDQVTAALMKLVHENHPVYVQWITDEELAANPAIVKSK
CCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCEEEEEECCHHHCCCCCEEECC
NVRPPMGLGRVSLVCIGENSSVDSQPCGGTHVSETQEVGAIHIAKIEKKGKENRRFRIRF
CCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHCCEEEEEEEHHCCCCCCEEEEEE
AAPEDRAFA
ECCCCCCCC
>Mature Secondary Structure 
TRSVTALFREDFYLSTCEASVTAILEDGGIELDQTCFYATSGGQPGDKGFLEREDGSRI
CCCHHHHHHCCCEEEECCCEEEEEEECCCCEECCEEEEEECCCCCCCCCCEECCCCCEE
DIIDTRHGATKDIILHIPAEGARMPDVGEKLVLHIDWPRRYRLMRMHTACHLLSVVCPFP
EEEECCCCCCCEEEEEECCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCC
ITGAAVGEEESRVDFDMSELIDKDQVTAALMKLVHENHPVYVQWITDEELAANPAIVKSK
CCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCEEEEEECCHHHCCCCCEEECC
NVRPPMGLGRVSLVCIGENSSVDSQPCGGTHVSETQEVGAIHIAKIEKKGKENRRFRIRF
CCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHCCEEEEEEEHHCCCCCCEEEEEE
AAPEDRAFA
ECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; @ALAN01.txt*L-alanine!; tRNAAla [C]

Specific reaction: ATP + @ALAN01.txt*L-alanine! + tRNAAla = AMP + diphosphate + L-alanyl-tRNAAla [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA