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The map label for this gene is sseA [H]

Identifier: 150396581

GI number: 150396581

Start: 1446142

End: 1447005

Strand: Direct

Name: sseA [H]

Synonym: Smed_1365

Alternate gene names: 150396581

Gene position: 1446142-1447005 (Clockwise)

Preceding gene: 150396580

Following gene: 150396582

Centisome position: 38.24

GC content: 61.23

Gene sequence:

>864_bases
ATGAACGTTGAAAACGCTAACAGGAGCGACTTTGTCGTTTCTGCAGACTGGCTTGAGCAGCGCCTCTCCGATCCGGCAGT
GAGAATCGTCGACGCCTCCTGGTACCTGCCGGCGCAGAACCGCGATCCGAAAGCCGAGTATGCGGCCGCGCATTTTCCCG
GGGCGGTCTTCTTCGATCAAGACGCGATTGCGGATCAGACGAGCGTGCTGCCGCACACCCTGCCCTCTCCCGAAGCATTC
GCCAAGGCTGTGGGTGCAATGGGAATCGGCGAGAACGACGTCATCGTCGTTTATGACGGTCCGGGTATTTTCACAGCGCC
GAGGGTCTGGTGGATGCTCCGCGTCATGGGTGCCAGGAATGTCTTCGTTCTCGATGGCGGCATGGACGGCTGGAAAGCGG
AGGGACGGCCGGTCACGGCACAGTTACCCAATCCCGAGCCGCGTGTGTTCAAGGCTTCGATCGACCGCCGTGCGGTTACC
TCGTTCGAGCGCATGAAGGACGTCGTCGAAAACCGGCTCGCCCAGATTGCCGATGCGCGGAGCGCCGGACGCTTCTCCGG
TGAGGAACCGGAACCCAGGGCAGGCATGCGGTCCGGCCATATGCCGGGTGCAAGGAGCCTGCCCTCAGGCGTGTTCTCGG
AGGGGGGCAAGTTCAAGGATCTGGATCAACTCAGCCGGACTTTTGCAGATGCCGGCATCGATCTGTCAAAACCGGTCGTC
ACGACCTGCGGCTCCGGCGTAACCGCCGCCATCATTACGCTTGCGCTTCAGTCCTTGGGACATAAGGACAACACGCTTTA
TGATGGCTCCTGGTCGGAATGGGGCAGCCGTCCCGACACTCCGGTTGCAACCGGTAAAGAGTGA

Upstream 100 bases:

>100_bases
AAAGGTAAAGAAAACCGGCGTTTCCGAATACGATTCGCGGCGCCCGAGGATCGGGCCTTCGCCTGAGCGCTTTTTATGAC
CATGCCAGTGGAGTAGAACG

Downstream 100 bases:

>100_bases
GAGCCATGCAAAGGTCGAAGCCTCCCGCGATCACGGCACACGTGACGGAACTCGAAATGACGTCGCCGCCAAAGCAAAGC
CTTCCCATGCCTATCAATAT

Product: rhodanese domain-containing protein

Products: NA

Alternate protein names: MST; Rhodanese-like protein [H]

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MNVENANRSDFVVSADWLEQRLSDPAVRIVDASWYLPAQNRDPKAEYAAAHFPGAVFFDQDAIADQTSVLPHTLPSPEAF
AKAVGAMGIGENDVIVVYDGPGIFTAPRVWWMLRVMGARNVFVLDGGMDGWKAEGRPVTAQLPNPEPRVFKASIDRRAVT
SFERMKDVVENRLAQIADARSAGRFSGEEPEPRAGMRSGHMPGARSLPSGVFSEGGKFKDLDQLSRTFADAGIDLSKPVV
TTCGSGVTAAIITLALQSLGHKDNTLYDGSWSEWGSRPDTPVATGKE

Sequences:

>Translated_287_residues
MNVENANRSDFVVSADWLEQRLSDPAVRIVDASWYLPAQNRDPKAEYAAAHFPGAVFFDQDAIADQTSVLPHTLPSPEAF
AKAVGAMGIGENDVIVVYDGPGIFTAPRVWWMLRVMGARNVFVLDGGMDGWKAEGRPVTAQLPNPEPRVFKASIDRRAVT
SFERMKDVVENRLAQIADARSAGRFSGEEPEPRAGMRSGHMPGARSLPSGVFSEGGKFKDLDQLSRTFADAGIDLSKPVV
TTCGSGVTAAIITLALQSLGHKDNTLYDGSWSEWGSRPDTPVATGKE
>Mature_287_residues
MNVENANRSDFVVSADWLEQRLSDPAVRIVDASWYLPAQNRDPKAEYAAAHFPGAVFFDQDAIADQTSVLPHTLPSPEAF
AKAVGAMGIGENDVIVVYDGPGIFTAPRVWWMLRVMGARNVFVLDGGMDGWKAEGRPVTAQLPNPEPRVFKASIDRRAVT
SFERMKDVVENRLAQIADARSAGRFSGEEPEPRAGMRSGHMPGARSLPSGVFSEGGKFKDLDQLSRTFADAGIDLSKPVV
TTCGSGVTAAIITLALQSLGHKDNTLYDGSWSEWGSRPDTPVATGKE

Specific function: Transfers a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. Its participation in detoxification of cyanide may be small. May be involved in the enhancement of serine sensitivity [H]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI17402865, Length=285, Percent_Identity=44.2105263157895, Blast_Score=229, Evalue=1e-60,
Organism=Homo sapiens, GI194473668, Length=284, Percent_Identity=40.1408450704225, Blast_Score=229, Evalue=2e-60,
Organism=Homo sapiens, GI61835204, Length=284, Percent_Identity=40.1408450704225, Blast_Score=229, Evalue=3e-60,
Organism=Homo sapiens, GI194473681, Length=284, Percent_Identity=40.1408450704225, Blast_Score=229, Evalue=3e-60,
Organism=Escherichia coli, GI87082121, Length=278, Percent_Identity=42.4460431654676, Blast_Score=231, Evalue=3e-62,
Organism=Escherichia coli, GI87081967, Length=271, Percent_Identity=25.0922509225092, Blast_Score=84, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71997283, Length=306, Percent_Identity=30.3921568627451, Blast_Score=124, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17561888, Length=301, Percent_Identity=29.2358803986711, Blast_Score=112, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17543836, Length=273, Percent_Identity=28.5714285714286, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17543838, Length=273, Percent_Identity=28.5714285714286, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI115534702, Length=213, Percent_Identity=28.169014084507, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17559150, Length=261, Percent_Identity=24.5210727969349, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324825, Length=272, Percent_Identity=35.6617647058824, Blast_Score=165, Evalue=9e-42,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.2 [H]

Molecular weight: Translated: 31034; Mature: 31034

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVENANRSDFVVSADWLEQRLSDPAVRIVDASWYLPAQNRDPKAEYAAAHFPGAVFFDQ
CCCCCCCCCCEEEEHHHHHHHHCCCCEEEEECEEECCCCCCCCCHHHHHHHCCCEEEECC
DAIADQTSVLPHTLPSPEAFAKAVGAMGIGENDVIVVYDGPGIFTAPRVWWMLRVMGARN
HHHCCHHHCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCE
VFVLDGGMDGWKAEGRPVTAQLPNPEPRVFKASIDRRAVTSFERMKDVVENRLAQIADAR
EEEEECCCCCCCCCCCEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAGRFSGEEPEPRAGMRSGHMPGARSLPSGVFSEGGKFKDLDQLSRTFADAGIDLSKPVV
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHCCCCCCCCHH
TTCGSGVTAAIITLALQSLGHKDNTLYDGSWSEWGSRPDTPVATGKE
HHCCCCHHHHHHHHHHHHCCCCCCEEECCCHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MNVENANRSDFVVSADWLEQRLSDPAVRIVDASWYLPAQNRDPKAEYAAAHFPGAVFFDQ
CCCCCCCCCCEEEEHHHHHHHHCCCCEEEEECEEECCCCCCCCCHHHHHHHCCCEEEECC
DAIADQTSVLPHTLPSPEAFAKAVGAMGIGENDVIVVYDGPGIFTAPRVWWMLRVMGARN
HHHCCHHHCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCE
VFVLDGGMDGWKAEGRPVTAQLPNPEPRVFKASIDRRAVTSFERMKDVVENRLAQIADAR
EEEEECCCCCCCCCCCEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAGRFSGEEPEPRAGMRSGHMPGARSLPSGVFSEGGKFKDLDQLSRTFADAGIDLSKPVV
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHCCCCCCCCHH
TTCGSGVTAAIITLALQSLGHKDNTLYDGSWSEWGSRPDTPVATGKE
HHCCCCHHHHHHHHHHHHCCCCCCEEECCCHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]