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The map label for this gene is eno [H]

Identifier: 150396293

GI number: 150396293

Start: 1140235

End: 1141509

Strand: Direct

Name: eno [H]

Synonym: Smed_1072

Alternate gene names: 150396293

Gene position: 1140235-1141509 (Clockwise)

Preceding gene: 150396292

Following gene: 150396294

Centisome position: 30.15

GC content: 62.9

Gene sequence:

>1275_bases
ATGACTGCAATCATCGACATCATCGGCCGAGAGATCCTCGACAGCCGCGGCAACCCGACCGTCGAGGTCGATGTCCATCT
CGAGGACGGCAGTTTCGGTCGCGCGGCGGTTCCCTCGGGCGCCTCGACCGGTGCTCATGAAGCGGTCGAACTGCGCGATG
GCGGCACGCGCTATCTCGGCAAGGGCGTAGAGCGCGCCGTCGATGCGGTGAACGGCGAGATCTTCGAAGCGATCGGCGGC
CTCGACGCTGAAAACCAGATCCAGATCGACAGAACCATGTTCGAGCTCGACGGCACCCCGAACAAGTCGCGCCTTGGCGC
CAACGCCATTCTGGGCGTTTCCCTCGCCGTGGCGAAGGCCGCTGCGGAAGCCGCCGGGCTGCCGCTCTACCGCTATGTCG
GCGGACCGAACGCGCATCTGCTTCCGGTGCCGATGATGAACATCATCAATGGCGGTGCGCATGCCGACAATCCGATCGAC
TTTCAGGAATTCATGATCATGCCGGTCGGAGCCGAAACCCTGAAGGACGCCGTCCGCATGGGATCGGAAGTCTTCCACAC
GCTGAAGAAGCAGCTTGCGGCGGATGGCCACAACACCAATGTCGGTGACGAAGGCGGCTTCGCGCCCGGCCTCGCCTCAG
CGCCGGCGGCTCTCGACTTCATCATGAAGTCGATCGAGAAGGCCGGCTACAGGCCCGGCGAAGACATGTATGTCGCTCTC
GACTGCGCTTCGACGGAATTCTTCAAGGACGGCAAATACGTCCTCGAGGGTGAAGGCCGCACGCTGGAGCCGGGCGCCAT
GGCCGAATACCTGGCGGAGCTTGCCGGCAAATATCCGATCGTCTCGATCGAAGACGGCATGGCCGAAGATGATTGGGACG
GCTGGAAAGCCCTGACGGACCTCATCGGCAACAAGTGCCAGCTCGTCGGCGACGACCTGTTCGTGACCAATTCCGCGCGT
CTGCGCGACGGCATCAAGATGGGCGTTGCCAACTCGATCCTCGTCAAGGTCAATCAGATCGGTTCGCTTTCGGAAACGCT
CGACGCGGTCGAGACCGCGCACAAGGCGCGCTATACGGCCGTCATGTCGCACCGCTCCGGCGAAACCGAGGATTCGACGA
TTGCCGATCTCGCGGTCGCGACCAATTGCGGCCAGATCAAAACCGGTTCGCTCGCTCGCTCCGACCGGCTTGCCAAGTAC
AACCAGCTGATCCGTATCGAAGAGCAGCTCGGCCCGCAGGCGCAATATGCCGGCCGGTCGATCCTGCGCGGCTGA

Upstream 100 bases:

>100_bases
CCGCGTGTGAATTTTTCATGATGCGGGCCGGCGCCATTGTAATTTCGAGGCCGTGAATTAAGACAGGCCGACCATCATCA
TCGTCCACAAGGAAGAGATC

Downstream 100 bases:

>100_bases
TCGTTTAACAGGCGGGGGGCGAAATCGCCCCTCGTCCGCCTCGCAACATCTGTTCCCCGCAAGCGCAGGGGGCAAGCGAC
GCTTCGGCATCGGCTCCGCC

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MTAIIDIIGREILDSRGNPTVEVDVHLEDGSFGRAAVPSGASTGAHEAVELRDGGTRYLGKGVERAVDAVNGEIFEAIGG
LDAENQIQIDRTMFELDGTPNKSRLGANAILGVSLAVAKAAAEAAGLPLYRYVGGPNAHLLPVPMMNIINGGAHADNPID
FQEFMIMPVGAETLKDAVRMGSEVFHTLKKQLAADGHNTNVGDEGGFAPGLASAPAALDFIMKSIEKAGYRPGEDMYVAL
DCASTEFFKDGKYVLEGEGRTLEPGAMAEYLAELAGKYPIVSIEDGMAEDDWDGWKALTDLIGNKCQLVGDDLFVTNSAR
LRDGIKMGVANSILVKVNQIGSLSETLDAVETAHKARYTAVMSHRSGETEDSTIADLAVATNCGQIKTGSLARSDRLAKY
NQLIRIEEQLGPQAQYAGRSILRG

Sequences:

>Translated_424_residues
MTAIIDIIGREILDSRGNPTVEVDVHLEDGSFGRAAVPSGASTGAHEAVELRDGGTRYLGKGVERAVDAVNGEIFEAIGG
LDAENQIQIDRTMFELDGTPNKSRLGANAILGVSLAVAKAAAEAAGLPLYRYVGGPNAHLLPVPMMNIINGGAHADNPID
FQEFMIMPVGAETLKDAVRMGSEVFHTLKKQLAADGHNTNVGDEGGFAPGLASAPAALDFIMKSIEKAGYRPGEDMYVAL
DCASTEFFKDGKYVLEGEGRTLEPGAMAEYLAELAGKYPIVSIEDGMAEDDWDGWKALTDLIGNKCQLVGDDLFVTNSAR
LRDGIKMGVANSILVKVNQIGSLSETLDAVETAHKARYTAVMSHRSGETEDSTIADLAVATNCGQIKTGSLARSDRLAKY
NQLIRIEEQLGPQAQYAGRSILRG
>Mature_423_residues
TAIIDIIGREILDSRGNPTVEVDVHLEDGSFGRAAVPSGASTGAHEAVELRDGGTRYLGKGVERAVDAVNGEIFEAIGGL
DAENQIQIDRTMFELDGTPNKSRLGANAILGVSLAVAKAAAEAAGLPLYRYVGGPNAHLLPVPMMNIINGGAHADNPIDF
QEFMIMPVGAETLKDAVRMGSEVFHTLKKQLAADGHNTNVGDEGGFAPGLASAPAALDFIMKSIEKAGYRPGEDMYVALD
CASTEFFKDGKYVLEGEGRTLEPGAMAEYLAELAGKYPIVSIEDGMAEDDWDGWKALTDLIGNKCQLVGDDLFVTNSARL
RDGIKMGVANSILVKVNQIGSLSETLDAVETAHKARYTAVMSHRSGETEDSTIADLAVATNCGQIKTGSLARSDRLAKYN
QLIRIEEQLGPQAQYAGRSILRG

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI5803011, Length=433, Percent_Identity=50.8083140877598, Blast_Score=402, Evalue=1e-112,
Organism=Homo sapiens, GI4503571, Length=432, Percent_Identity=49.537037037037, Blast_Score=399, Evalue=1e-111,
Organism=Homo sapiens, GI301897477, Length=433, Percent_Identity=49.8845265588915, Blast_Score=395, Evalue=1e-110,
Organism=Homo sapiens, GI301897469, Length=433, Percent_Identity=49.8845265588915, Blast_Score=395, Evalue=1e-110,
Organism=Homo sapiens, GI301897479, Length=431, Percent_Identity=45.9396751740139, Blast_Score=347, Evalue=2e-95,
Organism=Homo sapiens, GI169201331, Length=337, Percent_Identity=25.2225519287834, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI169201757, Length=337, Percent_Identity=25.2225519287834, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI239744207, Length=337, Percent_Identity=25.2225519287834, Blast_Score=100, Evalue=3e-21,
Organism=Escherichia coli, GI1789141, Length=430, Percent_Identity=62.5581395348837, Blast_Score=522, Evalue=1e-149,
Organism=Caenorhabditis elegans, GI71995829, Length=434, Percent_Identity=50.6912442396313, Blast_Score=389, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17536383, Length=434, Percent_Identity=50.6912442396313, Blast_Score=389, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI32563855, Length=190, Percent_Identity=46.3157894736842, Blast_Score=170, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6323985, Length=439, Percent_Identity=49.4305239179954, Blast_Score=388, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6324974, Length=439, Percent_Identity=49.2027334851936, Blast_Score=386, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6324969, Length=439, Percent_Identity=49.2027334851936, Blast_Score=386, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6321693, Length=440, Percent_Identity=47.9545454545455, Blast_Score=374, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6321968, Length=440, Percent_Identity=48.4090909090909, Blast_Score=362, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580918, Length=433, Percent_Identity=48.729792147806, Blast_Score=359, Evalue=2e-99,
Organism=Drosophila melanogaster, GI24580916, Length=433, Percent_Identity=48.729792147806, Blast_Score=359, Evalue=2e-99,
Organism=Drosophila melanogaster, GI24580920, Length=433, Percent_Identity=48.729792147806, Blast_Score=359, Evalue=2e-99,
Organism=Drosophila melanogaster, GI24580914, Length=433, Percent_Identity=48.729792147806, Blast_Score=359, Evalue=2e-99,
Organism=Drosophila melanogaster, GI281360527, Length=433, Percent_Identity=48.729792147806, Blast_Score=359, Evalue=2e-99,
Organism=Drosophila melanogaster, GI17137654, Length=433, Percent_Identity=48.729792147806, Blast_Score=359, Evalue=2e-99,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 45040; Mature: 44909

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAIIDIIGREILDSRGNPTVEVDVHLEDGSFGRAAVPSGASTGAHEAVELRDGGTRYLG
CCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHH
KGVERAVDAVNGEIFEAIGGLDAENQIQIDRTMFELDGTPNKSRLGANAILGVSLAVAKA
HHHHHHHHHHCHHHHHHHCCCCCCCEEEEEEEEEEECCCCCHHHCCCHHHHHHHHHHHHH
AAEAAGLPLYRYVGGPNAHLLPVPMMNIINGGAHADNPIDFQEFMIMPVGAETLKDAVRM
HHHHCCCCEEEECCCCCCEEECCCHHHHHCCCCCCCCCCCHHHEEEECCCHHHHHHHHHH
GSEVFHTLKKQLAADGHNTNVGDEGGFAPGLASAPAALDFIMKSIEKAGYRPGEDMYVAL
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEE
DCASTEFFKDGKYVLEGEGRTLEPGAMAEYLAELAGKYPIVSIEDGMAEDDWDGWKALTD
EECCCHHHCCCCEEEECCCCEECCHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHH
LIGNKCQLVGDDLFVTNSARLRDGIKMGVANSILVKVNQIGSLSETLDAVETAHKARYTA
HHCCCEEEECCCEEEECCHHHHHHHHHCCCCEEEEEEHHCCCHHHHHHHHHHHHHHHHHH
VMSHRSGETEDSTIADLAVATNCGQIKTGSLARSDRLAKYNQLIRIEEQLGPQAQYAGRS
HHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
ILRG
HCCC
>Mature Secondary Structure 
TAIIDIIGREILDSRGNPTVEVDVHLEDGSFGRAAVPSGASTGAHEAVELRDGGTRYLG
CHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHH
KGVERAVDAVNGEIFEAIGGLDAENQIQIDRTMFELDGTPNKSRLGANAILGVSLAVAKA
HHHHHHHHHHCHHHHHHHCCCCCCCEEEEEEEEEEECCCCCHHHCCCHHHHHHHHHHHHH
AAEAAGLPLYRYVGGPNAHLLPVPMMNIINGGAHADNPIDFQEFMIMPVGAETLKDAVRM
HHHHCCCCEEEECCCCCCEEECCCHHHHHCCCCCCCCCCCHHHEEEECCCHHHHHHHHHH
GSEVFHTLKKQLAADGHNTNVGDEGGFAPGLASAPAALDFIMKSIEKAGYRPGEDMYVAL
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEE
DCASTEFFKDGKYVLEGEGRTLEPGAMAEYLAELAGKYPIVSIEDGMAEDDWDGWKALTD
EECCCHHHCCCCEEEECCCCEECCHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHH
LIGNKCQLVGDDLFVTNSARLRDGIKMGVANSILVKVNQIGSLSETLDAVETAHKARYTA
HHCCCEEEECCCEEEECCHHHHHHHHHCCCCEEEEEEHHCCCHHHHHHHHHHHHHHHHHH
VMSHRSGETEDSTIADLAVATNCGQIKTGSLARSDRLAKYNQLIRIEEQLGPQAQYAGRS
HHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
ILRG
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]