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The map label for this gene is kdsA [H]

Identifier: 150396292

GI number: 150396292

Start: 1139283

End: 1140122

Strand: Direct

Name: kdsA [H]

Synonym: Smed_1071

Alternate gene names: 150396292

Gene position: 1139283-1140122 (Clockwise)

Preceding gene: 150396291

Following gene: 150396293

Centisome position: 30.12

GC content: 62.74

Gene sequence:

>840_bases
ATGGAAACCAATTCGAGAGTGGTCGTCGGCGAAGGTGCCGGCCAGGTGGTGTTCTCGCAGAAGGAGCGGCTGACGCTTAT
CGCCGGACCCTGCCAGATGGAAAGCCGCGAACACGCCTTCACGATTGCGGGAAAGCTTGTCGAGCTCTGTAGATCGCTCG
GGATCGGCCTCGTCTACAAGTCCTCTTTCGACAAGGCGAACCGCACGTCCCTCTCCGGAAAGCGCGGCATCGGGCTCGAC
AATGCGATGGAAGTCTTTGCCGACCTCAAGCGTGAATTCGGCTTCCCGGTCCTGACCGATATCCATACGGAAGAGCAATG
CGCGCTGGTCGCCGAGACGGTGGACATCCTGCAGATCCCCGCCTTTCTGTCGCGCCAGACTGACCTTCTCGTCGCTGCCG
CGAAGACGGGGCGCGCGATCAACGTCAAGAAGGGCCAGTTTCTCGCCCCCTGGGACATGAAGAACGTGCTCGCCAAATTT
ACGCAGAGTGGCAATCCCAATGTGCTCCTCTGCGAACGCGGCGCCTCCTTCGGCTATAATACGCTGGTCTCCGACATGCG
CTCGCTGCCGATCATGGCGGCACTCGGCGCTCCCGTGGTCTTCGATGCGACGCATTCCGTGCAGCAGCCAGGCGGCCAGG
GCGGTTCTTCCGGTGGCCAGCGCGAGTTCGTCGAAACGCTGGCGCGTGCGGCGGTGGCCGTCGGCGTCGCCGGGGTCTTC
GTTGAGACCCACGAGGATCCCGACAACGCTCCCTCGGACGGTCCGAACATGGTGCCGCTGAAGGACATGCCGCGGCTGCT
GGAGAAACTGCTTGCCTTTGACGCGATCGCCAAAGGGTGA

Upstream 100 bases:

>100_bases
TTTTCGCGCAGAATGATATCGAATTCGCAGAGATGGGCGGACGCCTCATCGTTCCGGAAGCGCCGGGGCAGGGTGTAACT
TTCGCCTTTGGAGTATGAAG

Downstream 100 bases:

>100_bases
GCGCGGACCGCGCCGCGTGTGAATTTTTCATGATGCGGGCCGGCGCCATTGTAATTTCGAGGCCGTGAATTAAGACAGGC
CGACCATCATCATCGTCCAC

Product: 2-dehydro-3-deoxyphosphooctonate aldolase

Products: NA

Alternate protein names: 3-deoxy-D-manno-octulosonic acid 8-phosphate synthase; KDO-8-phosphate synthase; KDO 8-P synthase; KDOPS; Phospho-2-dehydro-3-deoxyoctonate aldolase [H]

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
METNSRVVVGEGAGQVVFSQKERLTLIAGPCQMESREHAFTIAGKLVELCRSLGIGLVYKSSFDKANRTSLSGKRGIGLD
NAMEVFADLKREFGFPVLTDIHTEEQCALVAETVDILQIPAFLSRQTDLLVAAAKTGRAINVKKGQFLAPWDMKNVLAKF
TQSGNPNVLLCERGASFGYNTLVSDMRSLPIMAALGAPVVFDATHSVQQPGGQGGSSGGQREFVETLARAAVAVGVAGVF
VETHEDPDNAPSDGPNMVPLKDMPRLLEKLLAFDAIAKG

Sequences:

>Translated_279_residues
METNSRVVVGEGAGQVVFSQKERLTLIAGPCQMESREHAFTIAGKLVELCRSLGIGLVYKSSFDKANRTSLSGKRGIGLD
NAMEVFADLKREFGFPVLTDIHTEEQCALVAETVDILQIPAFLSRQTDLLVAAAKTGRAINVKKGQFLAPWDMKNVLAKF
TQSGNPNVLLCERGASFGYNTLVSDMRSLPIMAALGAPVVFDATHSVQQPGGQGGSSGGQREFVETLARAAVAVGVAGVF
VETHEDPDNAPSDGPNMVPLKDMPRLLEKLLAFDAIAKG
>Mature_279_residues
METNSRVVVGEGAGQVVFSQKERLTLIAGPCQMESREHAFTIAGKLVELCRSLGIGLVYKSSFDKANRTSLSGKRGIGLD
NAMEVFADLKREFGFPVLTDIHTEEQCALVAETVDILQIPAFLSRQTDLLVAAAKTGRAINVKKGQFLAPWDMKNVLAKF
TQSGNPNVLLCERGASFGYNTLVSDMRSLPIMAALGAPVVFDATHSVQQPGGQGGSSGGQREFVETLARAAVAVGVAGVF
VETHEDPDNAPSDGPNMVPLKDMPRLLEKLLAFDAIAKG

Specific function: Synthesis Of Kdo 8-P Which Is Required For Lipid A Maturation And Cellular Growth. [C]

COG id: COG2877

COG function: function code M; 3-deoxy-D-manno-octulosonic acid (KDO) 8-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the kdsA family [H]

Homologues:

Organism=Escherichia coli, GI1787466, Length=258, Percent_Identity=46.8992248062016, Blast_Score=244, Evalue=4e-66,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006218
- InterPro:   IPR006269 [H]

Pfam domain/function: PF00793 DAHP_synth_1 [H]

EC number: =2.5.1.55 [H]

Molecular weight: Translated: 29777; Mature: 29777

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METNSRVVVGEGAGQVVFSQKERLTLIAGPCQMESREHAFTIAGKLVELCRSLGIGLVYK
CCCCCEEEEECCCCCEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEE
SSFDKANRTSLSGKRGIGLDNAMEVFADLKREFGFPVLTDIHTEEQCALVAETVDILQIP
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHH
AFLSRQTDLLVAAAKTGRAINVKKGQFLAPWDMKNVLAKFTQSGNPNVLLCERGASFGYN
HHHCCCCCEEEEECCCCCEEEECCCCEECCCCHHHHHHHHHCCCCCCEEEECCCCCCCHH
TLVSDMRSLPIMAALGAPVVFDATHSVQQPGGQGGSSGGQREFVETLARAAVAVGVAGVF
HHHHHHHHCCHHHHHCCCEEEECCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEE
VETHEDPDNAPSDGPNMVPLKDMPRLLEKLLAFDAIAKG
EEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
METNSRVVVGEGAGQVVFSQKERLTLIAGPCQMESREHAFTIAGKLVELCRSLGIGLVYK
CCCCCEEEEECCCCCEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEE
SSFDKANRTSLSGKRGIGLDNAMEVFADLKREFGFPVLTDIHTEEQCALVAETVDILQIP
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHH
AFLSRQTDLLVAAAKTGRAINVKKGQFLAPWDMKNVLAKFTQSGNPNVLLCERGASFGYN
HHHCCCCCEEEEECCCCCEEEECCCCEECCCCHHHHHHHHHCCCCCCEEEECCCCCCCHH
TLVSDMRSLPIMAALGAPVVFDATHSVQQPGGQGGSSGGQREFVETLARAAVAVGVAGVF
HHHHHHHHCCHHHHHCCCEEEECCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEE
VETHEDPDNAPSDGPNMVPLKDMPRLLEKLLAFDAIAKG
EEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]