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The map label for this gene is chaA [C]

Identifier: 150395368

GI number: 150395368

Start: 153714

End: 154838

Strand: Direct

Name: chaA [C]

Synonym: Smed_0140

Alternate gene names: 150395368

Gene position: 153714-154838 (Clockwise)

Preceding gene: 150395363

Following gene: 150395370

Centisome position: 4.06

GC content: 60.44

Gene sequence:

>1125_bases
ATGATGAACGAAAGCTTCACTCCGGACGAAGAGGCGTGCTTGCTTGCAATCCTGAGATCGGAACGGCATCTCGTTGCCGC
TTTTCTTGCTGCTCTCGCCGGCGTTGCGCTCGAGCATCATCTGCTCGAAGCCGGTCGCGCTGCATCGCTCATGGGTGCGG
CCCTGATGGTGGCGGCGATCGTGCTTGCTTCCATGCGGGTCGCCCACCATGCCGAAGTGCTGGCCGCCAAGGTGGGCGAT
CCCTATGGCACGATGATCCTGACGCTCTCGGCGGTCCTTGTCGAGGTAATCATCCTCGCCATCATGATGACGGGCGAGAC
CTCTCCGACACTGGTGCGCGATACGATCTATTCGGCCGTCATGCTCGACGTCAACGGGATACTGGGTCTTGCGGCACTGC
TCGGCGGCCTCAAGCATGGGGAGCAGCCCTATAACGACGACTCGGCCCGCACCTACAGTGTCATGATCCTTACCGCCATG
GGCATTTCCATGCTCGTTCCCGAATTCGTGCCTCAGTCGAAATGGCACTACTATTCCGCCTTCACCATTGGCGCGATGAT
CGTTCTTTACACCCTTTTCCTCAGAATGCAGGTGGGCGTGCACAGCTACTTCTTCAGTTACAGCTATCCGCGTGCCAAAG
CGGGAAGTGCTGCAAGCCGGCTGGAAGGCGAGCCGGTGCTTCCTTCCGTCGTGGTGCTCGTCCTGGGCGTGATCGTGATC
GGCTTTCTCGCCGAAATCATGTCCGGGCTGCTTTCGGCAGGCATCAAGGGCACGGCCGCGCCGCCGGCATTGGCGGCGAT
CGTGGTTGCGGCGATCTCCGCGTCGCCCGAGGTCATGACGGCACTACGCGCGGCTCTTGCCAATCGCATGCAGGCAGTGG
TGAACATCGCGCTCGGCGCTTCGCTTTCGACTGTGATTCTGACCGTGCCGGTCATGGAGGCGATCGCGCTTTATACCGGT
CAGCCCTTCATCATGGCCATGTCCCCGGTGCAGACGGTGATGGTCGCGGTCACGCTCATTGCCGCCGCAATCAACCTGAA
TGACGGCCAGACCAATGCCATCGAAGGCATGACTCATTTCGTGCTCTTCGCGACCTTCATCATGCTTTCGGCTCTGGGGC
TCTAG

Upstream 100 bases:

>100_bases
AGAGCGGGCGAGGGAAAGTGCGTGCGGTATAAGGCTCGCGTCTCGCCCTAGTTCTTCGGAACCGATCGCCATCCTTTTAG
GTCGAGACGACCTCGAAGAG

Downstream 100 bases:

>100_bases
ATCGGCTCTCGCTGGAATCGTGAATGTGGTCGACCGGCTGCTGTTCCATTGACTTGCGCGCCACCACACGTTCATAGGCC
GCCTGTAGGGTCTGCCGCAC

Product: sodium/calcium exchanger membrane region

Products: Na (I) [Periplasm]; Ca (II) [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MMNESFTPDEEACLLAILRSERHLVAAFLAALAGVALEHHLLEAGRAASLMGAALMVAAIVLASMRVAHHAEVLAAKVGD
PYGTMILTLSAVLVEVIILAIMMTGETSPTLVRDTIYSAVMLDVNGILGLAALLGGLKHGEQPYNDDSARTYSVMILTAM
GISMLVPEFVPQSKWHYYSAFTIGAMIVLYTLFLRMQVGVHSYFFSYSYPRAKAGSAASRLEGEPVLPSVVVLVLGVIVI
GFLAEIMSGLLSAGIKGTAAPPALAAIVVAAISASPEVMTALRAALANRMQAVVNIALGASLSTVILTVPVMEAIALYTG
QPFIMAMSPVQTVMVAVTLIAAAINLNDGQTNAIEGMTHFVLFATFIMLSALGL

Sequences:

>Translated_374_residues
MMNESFTPDEEACLLAILRSERHLVAAFLAALAGVALEHHLLEAGRAASLMGAALMVAAIVLASMRVAHHAEVLAAKVGD
PYGTMILTLSAVLVEVIILAIMMTGETSPTLVRDTIYSAVMLDVNGILGLAALLGGLKHGEQPYNDDSARTYSVMILTAM
GISMLVPEFVPQSKWHYYSAFTIGAMIVLYTLFLRMQVGVHSYFFSYSYPRAKAGSAASRLEGEPVLPSVVVLVLGVIVI
GFLAEIMSGLLSAGIKGTAAPPALAAIVVAAISASPEVMTALRAALANRMQAVVNIALGASLSTVILTVPVMEAIALYTG
QPFIMAMSPVQTVMVAVTLIAAAINLNDGQTNAIEGMTHFVLFATFIMLSALGL
>Mature_374_residues
MMNESFTPDEEACLLAILRSERHLVAAFLAALAGVALEHHLLEAGRAASLMGAALMVAAIVLASMRVAHHAEVLAAKVGD
PYGTMILTLSAVLVEVIILAIMMTGETSPTLVRDTIYSAVMLDVNGILGLAALLGGLKHGEQPYNDDSARTYSVMILTAM
GISMLVPEFVPQSKWHYYSAFTIGAMIVLYTLFLRMQVGVHSYFFSYSYPRAKAGSAASRLEGEPVLPSVVVLVLGVIVI
GFLAEIMSGLLSAGIKGTAAPPALAAIVVAAISASPEVMTALRAALANRMQAVVNIALGASLSTVILTVPVMEAIALYTG
QPFIMAMSPVQTVMVAVTLIAAAINLNDGQTNAIEGMTHFVLFATFIMLSALGL

Specific function: Possible cation transporter [H]

COG id: COG0387

COG function: function code P; Ca2+/H+ antiporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium/potassium/calcium exchanger family [H]

Homologues:

Organism=Escherichia coli, GI1787468, Length=320, Percent_Identity=32.1875, Blast_Score=151, Evalue=6e-38,
Organism=Saccharomyces cerevisiae, GI6320075, Length=309, Percent_Identity=22.3300970873786, Blast_Score=68, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004713
- InterPro:   IPR004837 [H]

Pfam domain/function: PF01699 Na_Ca_ex [H]

EC number: NA

Molecular weight: Translated: 39235; Mature: 39235

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
6.1 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
6.1 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNESFTPDEEACLLAILRSERHLVAAFLAALAGVALEHHLLEAGRAASLMGAALMVAAI
CCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLASMRVAHHAEVLAAKVGDPYGTMILTLSAVLVEVIILAIMMTGETSPTLVRDTIYSAV
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
MLDVNGILGLAALLGGLKHGEQPYNDDSARTYSVMILTAMGISMLVPEFVPQSKWHYYSA
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
FTIGAMIVLYTLFLRMQVGVHSYFFSYSYPRAKAGSAASRLEGEPVLPSVVVLVLGVIVI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHH
GFLAEIMSGLLSAGIKGTAAPPALAAIVVAAISASPEVMTALRAALANRMQAVVNIALGA
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCC
SLSTVILTVPVMEAIALYTGQPFIMAMSPVQTVMVAVTLIAAAINLNDGQTNAIEGMTHF
HHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
VLFATFIMLSALGL
HHHHHHHHHHHHCC
>Mature Secondary Structure
MMNESFTPDEEACLLAILRSERHLVAAFLAALAGVALEHHLLEAGRAASLMGAALMVAAI
CCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLASMRVAHHAEVLAAKVGDPYGTMILTLSAVLVEVIILAIMMTGETSPTLVRDTIYSAV
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
MLDVNGILGLAALLGGLKHGEQPYNDDSARTYSVMILTAMGISMLVPEFVPQSKWHYYSA
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
FTIGAMIVLYTLFLRMQVGVHSYFFSYSYPRAKAGSAASRLEGEPVLPSVVVLVLGVIVI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHH
GFLAEIMSGLLSAGIKGTAAPPALAAIVVAAISASPEVMTALRAALANRMQAVVNIALGA
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCC
SLSTVILTVPVMEAIALYTGQPFIMAMSPVQTVMVAVTLIAAAINLNDGQTNAIEGMTHF
HHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
VLFATFIMLSALGL
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Cytoplasm]; Proton [Periplasm]; Ca (II) [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: Na (I) [Cytoplasm] + Proton [Periplasm] = Proton [Cytoplasm] + Na (I) [Periplasm] Ca (II) [Cytoplasm] + Proton [Periplasm] = Ca (II) [Periplasm] + Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]