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The map label for this gene is artI [H]

Identifier: 150395363

GI number: 150395363

Start: 149658

End: 150509

Strand: Direct

Name: artI [H]

Synonym: Smed_0135

Alternate gene names: 150395363

Gene position: 149658-150509 (Clockwise)

Preceding gene: 150395362

Following gene: 150395368

Centisome position: 3.96

GC content: 61.5

Gene sequence:

>852_bases
ATGCCGAGCGCATTCTTTCTGCGAATTTGCCGGATCTTCCTTCTCTCGGCCGTTCTTGGTGCCGGATCTGCCATGGCGCA
GCCCAAGGATCTGCCCAGGCTTTTTGACGCTCGCGAGCGCATTGCAAGGCCCGACTTGAGCGGGCTTGCCAGGCTTCGCT
TCCTGACGACGGTCGATTTCCCGCCCTTCAATTTCATCGATCAATCGGGCAAGCTGTCGGGCTTCCATGTCGACCTTGCC
CGCGAGATCTGCCGGGAGCTGGAGATCGAGGCAAAATGCCAGATCCAGGCGGTGACCTATCCGGAGCTCATGCCTGCACT
CGAACAGGGACAGGGCGAAGCGATCGCCGCCGGGATCTCCGTGACTTCGGAGTTGAGGCAGCGTTTCGCCTTCTCCCGAG
CCTTCATGCAACTGCCGGCCCGCTTCGTCGTCAACACGAAGGCTGCACCTGCGATCACCGGGCCTGCCGATCTCGCGCAG
AGGCCCGTCGGCGTCGTTTCCGGCACCATCCATGAGGCCATGCTGAAGGCGTTCTTCCCCGAGCTCGAGGCGAAAGGGTT
TTCCGACCGGGATTCGCTGCTTTCCGCGCTTCGCGAGGGGGCGGTTGCGGCTGCATTTTCCGACGGAATGCAGCTTTCCT
TCTGGGTATCCGGCAGCCTGGCCGGGGGATGCTGCGCCGTGATTGAAGGCTCCTATTTCTCCCAACGGTTCCTCGGCGAG
GGGCTGACGATCATGAACCGAAAGGCCGAGCCTGCCCTCACTCAGGCGATCGACCATGCGCTTTTGGCGCTCTCACGCAG
CGGCAGGCTCGAGGAGATCTACCTGCGCTATTTTCCGAATGGGATTTATTAG

Upstream 100 bases:

>100_bases
CGAGCGAGAGGTGCTGTAGCCAGTGCGGCCGCATCTCTATAGTCTTGATCATGAACCGAAACCGCGTCCTCCACCCGTGG
CTAACCCGAACTGGACCGCA

Downstream 100 bases:

>100_bases
AGCGGGATGAGGAAAAGCGTGCGCGGTTTTCCACCCGCATCTCGCGCCAGCCTAAGCAGCAGCGTCGGCGTTTGCTTATG
CGACTTGCTGTTCCCGCATG

Product: extracellular solute-binding protein

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MPSAFFLRICRIFLLSAVLGAGSAMAQPKDLPRLFDARERIARPDLSGLARLRFLTTVDFPPFNFIDQSGKLSGFHVDLA
REICRELEIEAKCQIQAVTYPELMPALEQGQGEAIAAGISVTSELRQRFAFSRAFMQLPARFVVNTKAAPAITGPADLAQ
RPVGVVSGTIHEAMLKAFFPELEAKGFSDRDSLLSALREGAVAAAFSDGMQLSFWVSGSLAGGCCAVIEGSYFSQRFLGE
GLTIMNRKAEPALTQAIDHALLALSRSGRLEEIYLRYFPNGIY

Sequences:

>Translated_283_residues
MPSAFFLRICRIFLLSAVLGAGSAMAQPKDLPRLFDARERIARPDLSGLARLRFLTTVDFPPFNFIDQSGKLSGFHVDLA
REICRELEIEAKCQIQAVTYPELMPALEQGQGEAIAAGISVTSELRQRFAFSRAFMQLPARFVVNTKAAPAITGPADLAQ
RPVGVVSGTIHEAMLKAFFPELEAKGFSDRDSLLSALREGAVAAAFSDGMQLSFWVSGSLAGGCCAVIEGSYFSQRFLGE
GLTIMNRKAEPALTQAIDHALLALSRSGRLEEIYLRYFPNGIY
>Mature_282_residues
PSAFFLRICRIFLLSAVLGAGSAMAQPKDLPRLFDARERIARPDLSGLARLRFLTTVDFPPFNFIDQSGKLSGFHVDLAR
EICRELEIEAKCQIQAVTYPELMPALEQGQGEAIAAGISVTSELRQRFAFSRAFMQLPARFVVNTKAAPAITGPADLAQR
PVGVVSGTIHEAMLKAFFPELEAKGFSDRDSLLSALREGAVAAAFSDGMQLSFWVSGSLAGGCCAVIEGSYFSQRFLGEG
LTIMNRKAEPALTQAIDHALLALSRSGRLEEIYLRYFPNGIY

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787088, Length=229, Percent_Identity=28.82096069869, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1787085, Length=235, Percent_Identity=23.4042553191489, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1788649, Length=234, Percent_Identity=26.4957264957265, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1788648, Length=237, Percent_Identity=26.1603375527426, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1788228, Length=222, Percent_Identity=22.972972972973, Blast_Score=75, Evalue=4e-15,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 30859; Mature: 30728

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSAFFLRICRIFLLSAVLGAGSAMAQPKDLPRLFDARERIARPDLSGLARLRFLTTVDF
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCC
PPFNFIDQSGKLSGFHVDLAREICRELEIEAKCQIQAVTYPELMPALEQGQGEAIAAGIS
CCCCCCCCCCCCCCEEHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHCCCCCEEEHHHH
VTSELRQRFAFSRAFMQLPARFVVNTKAAPAITGPADLAQRPVGVVSGTIHEAMLKAFFP
HHHHHHHHHHHHHHHHHCCHHHEEECCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHH
ELEAKGFSDRDSLLSALREGAVAAAFSDGMQLSFWVSGSLAGGCCAVIEGSYFSQRFLGE
HHHCCCCCCHHHHHHHHHHCCEEEECCCCCEEEEEECCCCCCCEEEEECCCHHHHHHHHC
GLTIMNRKAEPALTQAIDHALLALSRSGRLEEIYLRYFPNGIY
CCCEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCC
>Mature Secondary Structure 
PSAFFLRICRIFLLSAVLGAGSAMAQPKDLPRLFDARERIARPDLSGLARLRFLTTVDF
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCC
PPFNFIDQSGKLSGFHVDLAREICRELEIEAKCQIQAVTYPELMPALEQGQGEAIAAGIS
CCCCCCCCCCCCCCEEHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHCCCCCEEEHHHH
VTSELRQRFAFSRAFMQLPARFVVNTKAAPAITGPADLAQRPVGVVSGTIHEAMLKAFFP
HHHHHHHHHHHHHHHHHCCHHHEEECCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHH
ELEAKGFSDRDSLLSALREGAVAAAFSDGMQLSFWVSGSLAGGCCAVIEGSYFSQRFLGE
HHHCCCCCCHHHHHHHHHHCCEEEECCCCCEEEEEECCCCCCCEEEEECCCHHHHHHHHC
GLTIMNRKAEPALTQAIDHALLALSRSGRLEEIYLRYFPNGIY
CCCEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]