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The map label for this gene is wecB [C]

Identifier: 150010396

GI number: 150010396

Start: 4669057

End: 4670217

Strand: Direct

Name: wecB [C]

Synonym: BDI_3833

Alternate gene names: 150010396

Gene position: 4669057-4670217 (Clockwise)

Preceding gene: 150010395

Following gene: 150010397

Centisome position: 97.04

GC content: 44.27

Gene sequence:

>1161_bases
ATGGCAACATTTAAGAATAATGGGAAATTAAAATTGCTGATCATCGTTGGTACTCGTCCGGAGATTATCCGGTTGGCAGC
TGTTATTAATAAGTGCAGAAAATATTTTGACGTGGTATTGGCTCATACGGGTCAGAACTATGATTACAATTTGAACGGGA
TCTTTTTCAAGGATCTGAAGTTGGCGGATCCAGAGGTTTATCTGGATACCGTAGGGGATGATCTTGGTGCTACAATGGGT
AACATAATCGATAAGAGTTATAAGTTGATGGTTGAGTTAAAACCGGATGCTGTACTTGTGCTGGGTGATACCAACTCATG
CTTGAGTGTAATAGGAGCGAAGCGTTTACATATCCCTATCTTCCACATGGAGGCTGGTAATCGTTGTTTCGATGAGTGCC
TTCCAGAGGAAACAAACCGCCGTATAGTGGATATCATATCAGACGTGAATATTTGCTACAGCGAGCATGCTCGCCGCTAT
TTGAATGCCGCAGGTACTCCTAAGGAGCGTACTTATGTGGTAGGTTCTCCAATGGCTGAAGTCTTGCATAATAATTTGGA
GCGGATCAAGGCTAGTGATGTGCTAAGCCGATTGGGATTGGAGAAAGGCAAGTATATCCTTCTTTCTGCTCATCGGGAGG
AGAATATCGATACGAAGGCTAATTTCTTTAACTTGATGAACGCAGTGAACGCTATGGCAGAGAAGTACGATTTACCGATC
TTGTATTCTTGTCATCCTCGTTCTAAGAAATATATTGAGCAACGGGGTTTCCGGTTCGATCCTCGTGTGATTCAACATCA
GCCCTTAGGCTTTCATGACTACAATCATCTTCAGATAAACGCTTTTGCGGTCGTATCTGATTCCGGAACGCTTCCGGAGG
AGAGCAGTTTTTTCCTGTCTATTGGCAATCCGATCCCTGCGGTATGTATTCGCACAAGTACGGAACGTCCAGAGGCATTG
GACAAAGGAAACTTTATCTTGGCTGGCATCACGACGGAGCAGGTACTGCAAGCGGTGGATGTGGCTGTTGAGATGAACCG
AGATGGAGCGTTGGGCTTTCCGGTTCCTAACTATATGGATGAGAATGTCTCGGTAAAGGTGGTGAAACTGATTCAGAGTT
ATACCGGGGTGGTAGATAAAATGGTTTGGAGAAAAGAATAA

Upstream 100 bases:

>100_bases
TCTCCGATACGGAGGATCTGGTAACGGTAATGTGGGCGAATGAGAGTTTTGATTTGAACCACCCGGATACCTATTTTGAA
CCTGTTAAAAAAGAATAGTG

Downstream 100 bases:

>100_bases
AGGTTTATAAGAATTTGGAGTGTATTAGTCGTGAATTTGATTATAACAAGGCTATGGAGAAATGAACCATAGATGGTTGA
ATGGATATAAAGAGGTTGTT

Product: putative UDP-N-acetylglucosamine 2-epimerase

Products: UDP-N-acetyl-D-mannosamine

Alternate protein names: NA

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MATFKNNGKLKLLIIVGTRPEIIRLAAVINKCRKYFDVVLAHTGQNYDYNLNGIFFKDLKLADPEVYLDTVGDDLGATMG
NIIDKSYKLMVELKPDAVLVLGDTNSCLSVIGAKRLHIPIFHMEAGNRCFDECLPEETNRRIVDIISDVNICYSEHARRY
LNAAGTPKERTYVVGSPMAEVLHNNLERIKASDVLSRLGLEKGKYILLSAHREENIDTKANFFNLMNAVNAMAEKYDLPI
LYSCHPRSKKYIEQRGFRFDPRVIQHQPLGFHDYNHLQINAFAVVSDSGTLPEESSFFLSIGNPIPAVCIRTSTERPEAL
DKGNFILAGITTEQVLQAVDVAVEMNRDGALGFPVPNYMDENVSVKVVKLIQSYTGVVDKMVWRKE

Sequences:

>Translated_386_residues
MATFKNNGKLKLLIIVGTRPEIIRLAAVINKCRKYFDVVLAHTGQNYDYNLNGIFFKDLKLADPEVYLDTVGDDLGATMG
NIIDKSYKLMVELKPDAVLVLGDTNSCLSVIGAKRLHIPIFHMEAGNRCFDECLPEETNRRIVDIISDVNICYSEHARRY
LNAAGTPKERTYVVGSPMAEVLHNNLERIKASDVLSRLGLEKGKYILLSAHREENIDTKANFFNLMNAVNAMAEKYDLPI
LYSCHPRSKKYIEQRGFRFDPRVIQHQPLGFHDYNHLQINAFAVVSDSGTLPEESSFFLSIGNPIPAVCIRTSTERPEAL
DKGNFILAGITTEQVLQAVDVAVEMNRDGALGFPVPNYMDENVSVKVVKLIQSYTGVVDKMVWRKE
>Mature_385_residues
ATFKNNGKLKLLIIVGTRPEIIRLAAVINKCRKYFDVVLAHTGQNYDYNLNGIFFKDLKLADPEVYLDTVGDDLGATMGN
IIDKSYKLMVELKPDAVLVLGDTNSCLSVIGAKRLHIPIFHMEAGNRCFDECLPEETNRRIVDIISDVNICYSEHARRYL
NAAGTPKERTYVVGSPMAEVLHNNLERIKASDVLSRLGLEKGKYILLSAHREENIDTKANFFNLMNAVNAMAEKYDLPIL
YSCHPRSKKYIEQRGFRFDPRVIQHQPLGFHDYNHLQINAFAVVSDSGTLPEESSFFLSIGNPIPAVCIRTSTERPEALD
KGNFILAGITTEQVLQAVDVAVEMNRDGALGFPVPNYMDENVSVKVVKLIQSYTGVVDKMVWRKE

Specific function: Catalyzes The Reversible Epimerization At C-2 Of UDP-N- Acetylglucosamine (UDP-Glcnac) And Thereby Provides Bacteria With UDP-N-Acetylmannosamine (UDP-Mannac), The Activated Donor Of Mannac Residues. Also Involved In Bacteriophage N4 Adsorption. [C]

COG id: COG0381

COG function: function code M; UDP-N-acetylglucosamine 2-epimerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UDP-N-acetylglucosamine 2-epimerase family [H]

Homologues:

Organism=Escherichia coli, GI48994967, Length=345, Percent_Identity=26.9565217391304, Blast_Score=95, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003331 [H]

Pfam domain/function: PF02350 Epimerase_2 [H]

EC number: 5.1.3.14

Molecular weight: Translated: 43403; Mature: 43271

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATFKNNGKLKLLIIVGTRPEIIRLAAVINKCRKYFDVVLAHTGQNYDYNLNGIFFKDLK
CCEECCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHEECCCCCEECCCEEEEEEEE
LADPEVYLDTVGDDLGATMGNIIDKSYKLMVELKPDAVLVLGDTNSCLSVIGAKRLHIPI
ECCCCEEEECCCCHHHHHHHHHHCCCEEEEEEECCCEEEEECCCHHHHHHHCCCEEEEEE
FHMEAGNRCFDECLPEETNRRIVDIISDVNICYSEHARRYLNAAGTPKERTYVVGSPMAE
EEECCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHH
VLHNNLERIKASDVLSRLGLEKGKYILLSAHREENIDTKANFFNLMNAVNAMAEKYDLPI
HHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCE
LYSCHPRSKKYIEQRGFRFDPRVIQHQPLGFHDYNHLQINAFAVVSDSGTLPEESSFFLS
EEECCCCHHHHHHHCCCCCCCHHCCCCCCCCCCCCEEEEEEEEEEECCCCCCCCCCEEEE
IGNPIPAVCIRTSTERPEALDKGNFILAGITTEQVLQAVDVAVEMNRDGALGFPVPNYMD
ECCCCCEEEEECCCCCCCCCCCCCEEEEECCHHHHHHHHHHHEEECCCCCCCCCCCCCCC
ENVSVKVVKLIQSYTGVVDKMVWRKE
CCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ATFKNNGKLKLLIIVGTRPEIIRLAAVINKCRKYFDVVLAHTGQNYDYNLNGIFFKDLK
CEECCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHEECCCCCEECCCEEEEEEEE
LADPEVYLDTVGDDLGATMGNIIDKSYKLMVELKPDAVLVLGDTNSCLSVIGAKRLHIPI
ECCCCEEEECCCCHHHHHHHHHHCCCEEEEEEECCCEEEEECCCHHHHHHHCCCEEEEEE
FHMEAGNRCFDECLPEETNRRIVDIISDVNICYSEHARRYLNAAGTPKERTYVVGSPMAE
EEECCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHH
VLHNNLERIKASDVLSRLGLEKGKYILLSAHREENIDTKANFFNLMNAVNAMAEKYDLPI
HHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCE
LYSCHPRSKKYIEQRGFRFDPRVIQHQPLGFHDYNHLQINAFAVVSDSGTLPEESSFFLS
EEECCCCHHHHHHHCCCCCCCHHCCCCCCCCCCCCEEEEEEEEEEECCCCCCCCCCEEEE
IGNPIPAVCIRTSTERPEALDKGNFILAGITTEQVLQAVDVAVEMNRDGALGFPVPNYMD
ECCCCCEEEEECCCCCCCCCCCCCEEEEECCHHHHHHHHHHHEEECCCCCCCCCCCCCCC
ENVSVKVVKLIQSYTGVVDKMVWRKE
CCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: UDP-N-acetylglucosamine [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.63 {UDP-N-acetylglucosamine}} 1 {UDP-N-acetylmannosamine}} 0.5 {UDP-N-acetylmannosamine}} [C]

Substrates: UDP-N-acetyl-D-glucosamine

Specific reaction: UDP-N-acetyl-D-glucosamine = UDP-N-acetyl-D-mannosamine

General reaction: Epimerization [C]

Inhibitor: PCMB [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA