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The map label for this gene is aprN [H]

Identifier: 150009301

GI number: 150009301

Start: 3210551

End: 3211933

Strand: Reverse

Name: aprN [H]

Synonym: BDI_2703

Alternate gene names: 150009301

Gene position: 3211933-3210551 (Counterclockwise)

Preceding gene: 150009302

Following gene: 150009300

Centisome position: 66.76

GC content: 49.82

Gene sequence:

>1383_bases
ATGAGTAAATATATAGGAATATTCGTTTTCGTATTCAGTTTGTTTTCTTTGGGAGTACATGCCGGGGAAAGTTTTCGTTT
TCGGGTGTATTTGAAAGATAAAGGGGATTCCGGTTATACGCTGGATAACCCGGAGGAATATCTTTCCAAGGAGTCTGTCG
AGCGACGGAACCGGCAAGGTATATCGGTGTCGGAATCGGATTTGCCGATCGCTCAAGCTTATCTGGATACCTTGTCCGCC
AATGGAGGAAAACCGGTGCTGGAGAGTAAATGGTTCTCTACGGTCGTGGTGTCCAGTCCGGATAGCTTGGTCGCTGAGCG
TCTCTCGCGCCTCCCGATCGTGGATTCCGTGAAATGGGTCTGGAAAGGTGACGAGGAAATCGATATTCCTAGGGAGGAGA
AAGATACGACACGGTTTATGCCAATAGATAAACCTGAGAAATCGCCTTACGGTTATGCCGAGGAGCAGATCAAGATGCTG
AACGGTATCAAATTGCATGAGGCAGGCTTTAAGGGTGAGGGTATGCGTGTGGCCGTGGTCGATGCCGGTTTCATGAACGT
GGATCGGATCAGCGTATTTGATTCCTTGCGGTTATTAGGAACGCATAACGTGGTTTTCCCCGGTCGAAGTGTTTTCATCG
GGGATGATCATGGGACGAAGGTCTTATCTTGCTTGGCCGCTGATGCGCCGGGCTTGATGGTCGGTACGGCTCCCCAAGCC
GAATATTGGCTGATAAAGAGCGAGGATAGCCGTTCGGAGTTTCCCATCGAGGAGGATTATTGGACGGCAGCTATGGAATT
TGCCGATAGCGTGGGAGTGGATGTGGTCTCTTCTTCGTTGGGGTATTTCTCTTTTGATGTAGAGGCCCTGAACTATCATC
AAGATCAATTGGACGGGAGGACTTCCTTGATCAGTCGTGCCGCTAAGATGGCTTCATCAAAAGGGATCTTGTTGTTTAGT
AGCGCCGGCAACGAGGGGGGCGGTAGCTGGGGAAAGATAACCTTTCCAGCGGATGCCCCGGATATATTGACAGTGGGCGC
TATCACGGATCGTAAGAAGAAAAGTAATTTCAGTTCTGTCGGCTTTACAGCGGATTACCGGGTGAAGCCGGATGTGGTTG
CCTTGGGTACGGGCTGTTGCGTGATAGATCCTACGGGGAATATCCGTTACGCGAACGGTACCTCTTTCGCTACGCCGATC
TTAGCGGGGTTGGGTGTTTGTCTATGGCAAGCGTTTCCCAGTCTGACGAATAAGGATATAATCTCTCTTTTGCAGCGTTT
CGGCAGTAAGTTTGAGCAACCGGACGCTGAGTTGGGATATGGCATCCCGGATGTGTACAAAGCTTATAAACAGGAGCGTA
AATATGCGGCCGAATCTAAATAA

Upstream 100 bases:

>100_bases
ACGAAGAATTATACATGAGCCCGGCCTATTCCAGTGCCGGGCTTTTTTGTTTATTCGGCGGTATCATCTATCTTTGTAAT
TCGTAATTGTGTGAGGTTCT

Downstream 100 bases:

>100_bases
CTTATCGGGTGAGAAAGAGGCTCTATCTTTGTTAGAGCTGAATACCCGCATACGGGGTGCGTTGTCGAGGGCTTTCCCGG
AGACTTGCTGGGTGCGTGCG

Product: subtilase family serine protease

Products: NA

Alternate protein names: Nattokinase [H]

Number of amino acids: Translated: 460; Mature: 459

Protein sequence:

>460_residues
MSKYIGIFVFVFSLFSLGVHAGESFRFRVYLKDKGDSGYTLDNPEEYLSKESVERRNRQGISVSESDLPIAQAYLDTLSA
NGGKPVLESKWFSTVVVSSPDSLVAERLSRLPIVDSVKWVWKGDEEIDIPREEKDTTRFMPIDKPEKSPYGYAEEQIKML
NGIKLHEAGFKGEGMRVAVVDAGFMNVDRISVFDSLRLLGTHNVVFPGRSVFIGDDHGTKVLSCLAADAPGLMVGTAPQA
EYWLIKSEDSRSEFPIEEDYWTAAMEFADSVGVDVVSSSLGYFSFDVEALNYHQDQLDGRTSLISRAAKMASSKGILLFS
SAGNEGGGSWGKITFPADAPDILTVGAITDRKKKSNFSSVGFTADYRVKPDVVALGTGCCVIDPTGNIRYANGTSFATPI
LAGLGVCLWQAFPSLTNKDIISLLQRFGSKFEQPDAELGYGIPDVYKAYKQERKYAAESK

Sequences:

>Translated_460_residues
MSKYIGIFVFVFSLFSLGVHAGESFRFRVYLKDKGDSGYTLDNPEEYLSKESVERRNRQGISVSESDLPIAQAYLDTLSA
NGGKPVLESKWFSTVVVSSPDSLVAERLSRLPIVDSVKWVWKGDEEIDIPREEKDTTRFMPIDKPEKSPYGYAEEQIKML
NGIKLHEAGFKGEGMRVAVVDAGFMNVDRISVFDSLRLLGTHNVVFPGRSVFIGDDHGTKVLSCLAADAPGLMVGTAPQA
EYWLIKSEDSRSEFPIEEDYWTAAMEFADSVGVDVVSSSLGYFSFDVEALNYHQDQLDGRTSLISRAAKMASSKGILLFS
SAGNEGGGSWGKITFPADAPDILTVGAITDRKKKSNFSSVGFTADYRVKPDVVALGTGCCVIDPTGNIRYANGTSFATPI
LAGLGVCLWQAFPSLTNKDIISLLQRFGSKFEQPDAELGYGIPDVYKAYKQERKYAAESK
>Mature_459_residues
SKYIGIFVFVFSLFSLGVHAGESFRFRVYLKDKGDSGYTLDNPEEYLSKESVERRNRQGISVSESDLPIAQAYLDTLSAN
GGKPVLESKWFSTVVVSSPDSLVAERLSRLPIVDSVKWVWKGDEEIDIPREEKDTTRFMPIDKPEKSPYGYAEEQIKMLN
GIKLHEAGFKGEGMRVAVVDAGFMNVDRISVFDSLRLLGTHNVVFPGRSVFIGDDHGTKVLSCLAADAPGLMVGTAPQAE
YWLIKSEDSRSEFPIEEDYWTAAMEFADSVGVDVVSSSLGYFSFDVEALNYHQDQLDGRTSLISRAAKMASSKGILLFSS
AGNEGGGSWGKITFPADAPDILTVGAITDRKKKSNFSSVGFTADYRVKPDVVALGTGCCVIDPTGNIRYANGTSFATPIL
AGLGVCLWQAFPSLTNKDIISLLQRFGSKFEQPDAELGYGIPDVYKAYKQERKYAAESK

Specific function: Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides. Subtilisin NAT also has fibrinolytic activity [H]

COG id: COG1404

COG function: function code O; Subtilisin-like serine proteases

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S8 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000209
- InterPro:   IPR022398
- InterPro:   IPR015500
- InterPro:   IPR009020
- InterPro:   IPR010259 [H]

Pfam domain/function: PF05922 Inhibitor_I9; PF00082 Peptidase_S8 [H]

EC number: =3.4.21.62 [H]

Molecular weight: Translated: 50476; Mature: 50345

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00136 SUBTILASE_ASP ; PS00138 SUBTILASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKYIGIFVFVFSLFSLGVHAGESFRFRVYLKDKGDSGYTLDNPEEYLSKESVERRNRQG
CCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCC
ISVSESDLPIAQAYLDTLSANGGKPVLESKWFSTVVVSSPDSLVAERLSRLPIVDSVKWV
CCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHCCCCCCCEEEE
WKGDEEIDIPREEKDTTRFMPIDKPEKSPYGYAEEQIKMLNGIKLHEAGFKGEGMRVAVV
ECCCCCCCCCCCCCCCCEECCCCCCCCCCCCCHHHHHHHHCCEEEECCCCCCCCEEEEEE
DAGFMNVDRISVFDSLRLLGTHNVVFPGRSVFIGDDHGTKVLSCLAADAPGLMVGTAPQA
ECCCCCCHHHHHHHHHHHHCCCCEEECCCEEEEECCCCHHHHHHHHCCCCCEEEECCCCC
EYWLIKSEDSRSEFPIEEDYWTAAMEFADSVGVDVVSSSLGYFSFDVEALNYHQDQLDGR
EEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHCCCCEEEEEHHHHHCCHHHHCCH
TSLISRAAKMASSKGILLFSSAGNEGGGSWGKITFPADAPDILTVGAITDRKKKSNFSSV
HHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEECCCCCCEEEEECCCCCHHHCCCCCC
GFTADYRVKPDVVALGTGCCVIDPTGNIRYANGTSFATPILAGLGVCLWQAFPSLTNKDI
CEEEECEECCCEEEEECCEEEECCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHH
ISLLQRFGSKFEQPDAELGYGIPDVYKAYKQERKYAAESK
HHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SKYIGIFVFVFSLFSLGVHAGESFRFRVYLKDKGDSGYTLDNPEEYLSKESVERRNRQG
CCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCC
ISVSESDLPIAQAYLDTLSANGGKPVLESKWFSTVVVSSPDSLVAERLSRLPIVDSVKWV
CCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHCCCCCCCEEEE
WKGDEEIDIPREEKDTTRFMPIDKPEKSPYGYAEEQIKMLNGIKLHEAGFKGEGMRVAVV
ECCCCCCCCCCCCCCCCEECCCCCCCCCCCCCHHHHHHHHCCEEEECCCCCCCCEEEEEE
DAGFMNVDRISVFDSLRLLGTHNVVFPGRSVFIGDDHGTKVLSCLAADAPGLMVGTAPQA
ECCCCCCHHHHHHHHHHHHCCCCEEECCCEEEEECCCCHHHHHHHHCCCCCEEEECCCCC
EYWLIKSEDSRSEFPIEEDYWTAAMEFADSVGVDVVSSSLGYFSFDVEALNYHQDQLDGR
EEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHCCCCEEEEEHHHHHCCHHHHCCH
TSLISRAAKMASSKGILLFSSAGNEGGGSWGKITFPADAPDILTVGAITDRKKKSNFSSV
HHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEECCCCCCEEEEECCCCCHHHCCCCCC
GFTADYRVKPDVVALGTGCCVIDPTGNIRYANGTSFATPILAGLGVCLWQAFPSLTNKDI
CEEEECEECCCEEEEECCEEEECCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHH
ISLLQRFGSKFEQPDAELGYGIPDVYKAYKQERKYAAESK
HHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1369081 [H]