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The map label for this gene is metK

Identifier: 150009007

GI number: 150009007

Start: 2815756

End: 2817042

Strand: Reverse

Name: metK

Synonym: BDI_2403

Alternate gene names: 150009007

Gene position: 2817042-2815756 (Counterclockwise)

Preceding gene: 150009008

Following gene: 150009004

Centisome position: 58.55

GC content: 47.47

Gene sequence:

>1287_bases
ATGAGTTATTTATTCACCTCCGAATCGGTGTCTGAAGGGCACCCCGATAAAGTAGCCGATCAAATATCGGATGCTTTGCT
GGATGAGTTCTTGGCGTATGACAAGAATTCGAAAGTTGCTTGCGAAACCCTTGTTACAACCGGACAGGTTGTCTTGGCTG
GAGAAGTTAAATCAAGTGCGTATGTGGATGTGCAGGAAGTGGCACGTGGCGTGATCGAGAAGATTGGTTACACGAAAAGC
GAGTACCAGTTTGAAGCGAAATCATGTGGTGTGTTCTCTTCTATCCATGAGCAGAGTGGCGACATCAACCGTGGCGTAGA
GCGTGCGGATCCCTATGAGCAGGGTGCCGGCGACCAAGGTATGATGTTCGGTTATGCTACTAATGAGACTGAGAATTATA
TGCCTTTGGCGTTGGACTTATCTCATAGTTTATTATGGGAATTGGCGAAGATCCGTAAGGAAGAACCGGATTTGATGCCT
TATTTGCGTCCGGACGCTAAGAGTCAGGTTACGATTGAATATGATGATAACGGTAAGCCATTGCGTATCGATACGATTGT
GGTATCCACGCAGCATGATGAGTTTATCGGTCCGAAAGGTATTTCACAGAAGGAAGCGGATGAGGCTATGCAAAAGAGGA
TCGCCTTAGACGTGAAGAAGATATTGATACCTCGTGTGAAGGCTCAATATCCGGCTCATGTACAGGCTTTGTTTAATGAT
AATATCATTTATCACGTGAACCCGACCGGTAAGTTCGTGATCGGTGGTCCTCACGGTGATACCGGTTTGACCGGCCGTAA
GATCATCGTGGATACATACGGTGGCAAAGGTGCTCATGGCGGTGGTGCTTTCTCTGGAAAGGACCCTTCTAAGGTAGACC
GTTCGGCTGCTTATGCCGCTCGTCATATCGCTAAGAATATGGTTGCCGCCGGTGTGGCGGATGAGATGCTGGTACAGGTG
TCTTATGCGATCGGTGTGGCTAAACCGATGAATATTTTCGTGAATACTTTTGGCCGTGCGAAGGTGAATATGACGGACGG
AGAGATCGCCGCGAAGATCTGGGATATCTTCGATATGCGTCCGAAAGCGATCGAGGAGCGTTTGAAATTGCGTAACCCGA
TTTATTTCGAGACCGCTTCTTATGGCCATATGGGACGCAAACCCCAGACGGTGACGAAGACATTCTCTTCCCGTTATTTG
CAGAAGCCGGTTACTTGTGATGTGGAGTTATTCACATGGGAGAAATTGGATTATGCAGATAAGATCAAGGACGTGTTTGG
TCTATAA

Upstream 100 bases:

>100_bases
AATTCAGTCATAGATTAATAACTAACGAGCATTGTCAATTGCGACCTGTGGTTTATCCCCTAATTCTCAATTTTCAATTC
TCAATTTTCAATTTAAAACG

Downstream 100 bases:

>100_bases
AAAATAAGTGGAAAAAGTAAGCTGGCTTAAATGGCGAAGTATGTTAAGATACTTTGCCATTTAAGTTAAGATACTTAGCC
GATGAAGTTAAGATACTTTG

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MSYLFTSESVSEGHPDKVADQISDALLDEFLAYDKNSKVACETLVTTGQVVLAGEVKSSAYVDVQEVARGVIEKIGYTKS
EYQFEAKSCGVFSSIHEQSGDINRGVERADPYEQGAGDQGMMFGYATNETENYMPLALDLSHSLLWELAKIRKEEPDLMP
YLRPDAKSQVTIEYDDNGKPLRIDTIVVSTQHDEFIGPKGISQKEADEAMQKRIALDVKKILIPRVKAQYPAHVQALFND
NIIYHVNPTGKFVIGGPHGDTGLTGRKIIVDTYGGKGAHGGGAFSGKDPSKVDRSAAYAARHIAKNMVAAGVADEMLVQV
SYAIGVAKPMNIFVNTFGRAKVNMTDGEIAAKIWDIFDMRPKAIEERLKLRNPIYFETASYGHMGRKPQTVTKTFSSRYL
QKPVTCDVELFTWEKLDYADKIKDVFGL

Sequences:

>Translated_428_residues
MSYLFTSESVSEGHPDKVADQISDALLDEFLAYDKNSKVACETLVTTGQVVLAGEVKSSAYVDVQEVARGVIEKIGYTKS
EYQFEAKSCGVFSSIHEQSGDINRGVERADPYEQGAGDQGMMFGYATNETENYMPLALDLSHSLLWELAKIRKEEPDLMP
YLRPDAKSQVTIEYDDNGKPLRIDTIVVSTQHDEFIGPKGISQKEADEAMQKRIALDVKKILIPRVKAQYPAHVQALFND
NIIYHVNPTGKFVIGGPHGDTGLTGRKIIVDTYGGKGAHGGGAFSGKDPSKVDRSAAYAARHIAKNMVAAGVADEMLVQV
SYAIGVAKPMNIFVNTFGRAKVNMTDGEIAAKIWDIFDMRPKAIEERLKLRNPIYFETASYGHMGRKPQTVTKTFSSRYL
QKPVTCDVELFTWEKLDYADKIKDVFGL
>Mature_427_residues
SYLFTSESVSEGHPDKVADQISDALLDEFLAYDKNSKVACETLVTTGQVVLAGEVKSSAYVDVQEVARGVIEKIGYTKSE
YQFEAKSCGVFSSIHEQSGDINRGVERADPYEQGAGDQGMMFGYATNETENYMPLALDLSHSLLWELAKIRKEEPDLMPY
LRPDAKSQVTIEYDDNGKPLRIDTIVVSTQHDEFIGPKGISQKEADEAMQKRIALDVKKILIPRVKAQYPAHVQALFNDN
IIYHVNPTGKFVIGGPHGDTGLTGRKIIVDTYGGKGAHGGGAFSGKDPSKVDRSAAYAARHIAKNMVAAGVADEMLVQVS
YAIGVAKPMNIFVNTFGRAKVNMTDGEIAAKIWDIFDMRPKAIEERLKLRNPIYFETASYGHMGRKPQTVTKTFSSRYLQ
KPVTCDVELFTWEKLDYADKIKDVFGL

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI4557737, Length=389, Percent_Identity=50.8997429305913, Blast_Score=348, Evalue=7e-96,
Organism=Homo sapiens, GI5174529, Length=389, Percent_Identity=49.8714652956298, Blast_Score=342, Evalue=3e-94,
Organism=Escherichia coli, GI1789311, Length=426, Percent_Identity=52.3474178403756, Blast_Score=420, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17551082, Length=388, Percent_Identity=52.0618556701031, Blast_Score=360, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI17538494, Length=389, Percent_Identity=52.9562982005141, Blast_Score=355, Evalue=2e-98,
Organism=Caenorhabditis elegans, GI25145633, Length=387, Percent_Identity=51.6795865633075, Blast_Score=352, Evalue=2e-97,
Organism=Caenorhabditis elegans, GI17538498, Length=389, Percent_Identity=52.4421593830334, Blast_Score=352, Evalue=2e-97,
Organism=Caenorhabditis elegans, GI32565905, Length=390, Percent_Identity=47.1794871794872, Blast_Score=297, Evalue=8e-81,
Organism=Caenorhabditis elegans, GI25145635, Length=340, Percent_Identity=49.1176470588235, Blast_Score=284, Evalue=7e-77,
Organism=Caenorhabditis elegans, GI32565911, Length=341, Percent_Identity=50.1466275659824, Blast_Score=283, Evalue=8e-77,
Organism=Caenorhabditis elegans, GI32565903, Length=316, Percent_Identity=49.6835443037975, Blast_Score=259, Evalue=2e-69,
Organism=Caenorhabditis elegans, GI32565909, Length=78, Percent_Identity=62.8205128205128, Blast_Score=103, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6323209, Length=400, Percent_Identity=48.5, Blast_Score=338, Evalue=1e-93,
Organism=Saccharomyces cerevisiae, GI6320710, Length=388, Percent_Identity=49.4845360824742, Blast_Score=336, Evalue=3e-93,
Organism=Drosophila melanogaster, GI45552159, Length=390, Percent_Identity=51.025641025641, Blast_Score=328, Evalue=3e-90,
Organism=Drosophila melanogaster, GI24580529, Length=390, Percent_Identity=51.025641025641, Blast_Score=328, Evalue=3e-90,
Organism=Drosophila melanogaster, GI24580537, Length=390, Percent_Identity=51.025641025641, Blast_Score=328, Evalue=3e-90,
Organism=Drosophila melanogaster, GI24580533, Length=390, Percent_Identity=51.025641025641, Blast_Score=328, Evalue=3e-90,
Organism=Drosophila melanogaster, GI24580535, Length=390, Percent_Identity=51.025641025641, Blast_Score=328, Evalue=3e-90,
Organism=Drosophila melanogaster, GI24580531, Length=390, Percent_Identity=51.025641025641, Blast_Score=328, Evalue=3e-90,
Organism=Drosophila melanogaster, GI24580527, Length=389, Percent_Identity=50.1285347043702, Blast_Score=323, Evalue=1e-88,
Organism=Drosophila melanogaster, GI24580525, Length=389, Percent_Identity=50.1285347043702, Blast_Score=323, Evalue=1e-88,
Organism=Drosophila melanogaster, GI24580541, Length=389, Percent_Identity=48.0719794344473, Blast_Score=300, Evalue=1e-81,
Organism=Drosophila melanogaster, GI24580539, Length=119, Percent_Identity=49.5798319327731, Blast_Score=116, Evalue=2e-26,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_PARD8 (A6LEL4)

Other databases:

- EMBL:   CP000140
- RefSeq:   YP_001303750.1
- ProteinModelPortal:   A6LEL4
- SMR:   A6LEL4
- STRING:   A6LEL4
- GeneID:   5307552
- GenomeReviews:   CP000140_GR
- KEGG:   pdi:BDI_2403
- eggNOG:   COG0192
- HOGENOM:   HBG443662
- OMA:   IYYAHRL
- ProtClustDB:   PRK05250
- BioCyc:   PDIS435591:BDI_2403-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 47345; Mature: 47214

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYLFTSESVSEGHPDKVADQISDALLDEFLAYDKNSKVACETLVTTGQVVLAGEVKSSA
CCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHCCCCEEEEECCCCCC
YVDVQEVARGVIEKIGYTKSEYQFEAKSCGVFSSIHEQSGDINRGVERADPYEQGAGDQG
EEEHHHHHHHHHHHHCCCCHHEEECHHHCCHHHHHHHCCCCHHCCCCCCCCHHHCCCCCC
MMFGYATNETENYMPLALDLSHSLLWELAKIRKEEPDLMPYLRPDAKSQVTIEYDDNGKP
EEEEEECCCCCCCEEEEEECHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCE
LRIDTIVVSTQHDEFIGPKGISQKEADEAMQKRIALDVKKILIPRVKAQYPAHVQALFND
EEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHEECC
NIIYHVNPTGKFVIGGPHGDTGLTGRKIIVDTYGGKGAHGGGAFSGKDPSKVDRSAAYAA
CEEEEECCCCEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHH
RHIAKNMVAAGVADEMLVQVSYAIGVAKPMNIFVNTFGRAKVNMTDGEIAAKIWDIFDMR
HHHHHHHHHHCHHHHHHHHHHHHHCCCCHHHHHEECCCCEEEECCCCHHHHHHHHHHCCC
PKAIEERLKLRNPIYFETASYGHMGRKPQTVTKTFSSRYLQKPVTCDVELFTWEKLDYAD
HHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEECCCCHHH
KIKDVFGL
HHHHHHCC
>Mature Secondary Structure 
SYLFTSESVSEGHPDKVADQISDALLDEFLAYDKNSKVACETLVTTGQVVLAGEVKSSA
CEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHCCCCEEEEECCCCCC
YVDVQEVARGVIEKIGYTKSEYQFEAKSCGVFSSIHEQSGDINRGVERADPYEQGAGDQG
EEEHHHHHHHHHHHHCCCCHHEEECHHHCCHHHHHHHCCCCHHCCCCCCCCHHHCCCCCC
MMFGYATNETENYMPLALDLSHSLLWELAKIRKEEPDLMPYLRPDAKSQVTIEYDDNGKP
EEEEEECCCCCCCEEEEEECHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCE
LRIDTIVVSTQHDEFIGPKGISQKEADEAMQKRIALDVKKILIPRVKAQYPAHVQALFND
EEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHEECC
NIIYHVNPTGKFVIGGPHGDTGLTGRKIIVDTYGGKGAHGGGAFSGKDPSKVDRSAAYAA
CEEEEECCCCEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHH
RHIAKNMVAAGVADEMLVQVSYAIGVAKPMNIFVNTFGRAKVNMTDGEIAAKIWDIFDMR
HHHHHHHHHHCHHHHHHHHHHHHHCCCCHHHHHEECCCCEEEECCCCHHHHHHHHHHCCC
PKAIEERLKLRNPIYFETASYGHMGRKPQTVTKTFSSRYLQKPVTCDVELFTWEKLDYAD
HHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEECCCCHHH
KIKDVFGL
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA