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The map label for this gene is leuC [H]

Identifier: 150008673

GI number: 150008673

Start: 2433375

End: 2434772

Strand: Reverse

Name: leuC [H]

Synonym: BDI_2063

Alternate gene names: 150008673

Gene position: 2434772-2433375 (Counterclockwise)

Preceding gene: 150008674

Following gene: 150008672

Centisome position: 50.6

GC content: 52.65

Gene sequence:

>1398_bases
ATGAGCAAGACATTATTTGAGAAAATCTGGGACAAACACGTGGTGGACACGTTGCCCGACGGAACCATACAATTGTATAT
CGACCGTCTGTATTGTCATGAGGTGACTAGCCCGCAGGCTTTCGCCGGATTACGTGCACGTGGATTGAAGCCCTTCCGTC
CCGCGCAAATCACTTGTATGCCGGACCACAATATCCCGACATTGAACCAAGATAAGCCTATCGAGGACCCTATATCCAAG
AATCAAGTGGATACCTTGGAAGAGAACGCGAAGTATTTCGGCTTGACGTATTACGGTCTTCAACATCCGAAGAATGGTAT
CATCCATGTGGTAGGTCCGGAGACAGGCTTGACGCAGCCGGGTATGACGATCGTTTGTGGCGACTCCCATACTTCTACGC
ACGGTGCCATGGGTGCTATCGCCTTCGGTATCGGTACGAGCGAGGTGGAGATGACCTTGGCTACCCAATGTATCATGCAG
CGTAAGCCGAAAACGATGCGTATCACGATCGATGGTGTACGCAAACCGGGCGTTACGGCGAAGGACTTTGCCTTATACAT
TATTAGTAAGATGAGTACCAGCGGTGCTACGGGCTATTTCGTGGAGTACGCCGGCGAGGCGATCCGTAATACGACCATGG
AGGAACGTCTGACGATCTGCAATCTTTCGATCGAGATGGGTGCCCGTGGTGGTATGATCGCTCCGGACGATGTTACTTTC
GATTATTTGAAAGACCGTGAGTTCGCCCCAAGAGGCGAGGAATGGGAGCGGAAGGTGAGCGAGTGGCGTCAGTTATATAG
TGATCCGGACGCTAAGTTCGATAAGGAGATCGTTTTCCACGCCGAGGATATCGACCCGATGGTTACGTACGGTACGAACC
CGGGCATGGGTATGAGTATCAGCAAAGATATCCCCTCCTTAGAAAGCGTGCCCGAGGCAGGCCGTATCTCTTATAAGAAG
TCTTTGGATTATATGGGCTTCAAGGCAGGCGAGTCTATGTTGGGTAAGAAAGTGGATTATGTCTTTTTAGGTAGCTGTAC
GAATGGCCGTATCGAGGATTTCCGTGCTTTCGCCTCTTTGGTGAAAGGAAAGAAGAAAGCGGATGATGTAACGGTTTGGC
TGGTTCCCGGCAGTTGGCAGGTAGAGCGGGAGATTCGTGCCGAGGGGATCGACAAGGTTTTGGCCGAGGCCGGCTTCGTC
CTTCGTCAACCGGGATGTTCCGCTTGTCTGGCGATGAATGAGGATAAGGTTCCGGCGGGTAAATATGCGGTCTCCACTTC
CAACCGTAATTTCGAGGGCCGCCAAGGACCGGGTGCGCGCACCATCTTAGCGGGGCCGCTTGTGGCCGCCGCCGCCGCTG
TGACAGGTAAGATCACGGACCCGAGAGAACTGATGTAA

Upstream 100 bases:

>100_bases
ATTATGGGTTTTCTGCGAATACCGATATTATCGCTGCTTCCGTGGAGGCTTTCGTGGACGCCATCAATAAGTTTATAGTA
GACTAAAAATAGACAAGACA

Downstream 100 bases:

>100_bases
AGACTAATCTAATAAAGTAGCATAAGATGAAAACAAAATTCAATATAATCACAAGCACTTGCGTACCTCTTCCTTTAGAG
AACGTGGATACCGACCAGAT

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MSKTLFEKIWDKHVVDTLPDGTIQLYIDRLYCHEVTSPQAFAGLRARGLKPFRPAQITCMPDHNIPTLNQDKPIEDPISK
NQVDTLEENAKYFGLTYYGLQHPKNGIIHVVGPETGLTQPGMTIVCGDSHTSTHGAMGAIAFGIGTSEVEMTLATQCIMQ
RKPKTMRITIDGVRKPGVTAKDFALYIISKMSTSGATGYFVEYAGEAIRNTTMEERLTICNLSIEMGARGGMIAPDDVTF
DYLKDREFAPRGEEWERKVSEWRQLYSDPDAKFDKEIVFHAEDIDPMVTYGTNPGMGMSISKDIPSLESVPEAGRISYKK
SLDYMGFKAGESMLGKKVDYVFLGSCTNGRIEDFRAFASLVKGKKKADDVTVWLVPGSWQVEREIRAEGIDKVLAEAGFV
LRQPGCSACLAMNEDKVPAGKYAVSTSNRNFEGRQGPGARTILAGPLVAAAAAVTGKITDPRELM

Sequences:

>Translated_465_residues
MSKTLFEKIWDKHVVDTLPDGTIQLYIDRLYCHEVTSPQAFAGLRARGLKPFRPAQITCMPDHNIPTLNQDKPIEDPISK
NQVDTLEENAKYFGLTYYGLQHPKNGIIHVVGPETGLTQPGMTIVCGDSHTSTHGAMGAIAFGIGTSEVEMTLATQCIMQ
RKPKTMRITIDGVRKPGVTAKDFALYIISKMSTSGATGYFVEYAGEAIRNTTMEERLTICNLSIEMGARGGMIAPDDVTF
DYLKDREFAPRGEEWERKVSEWRQLYSDPDAKFDKEIVFHAEDIDPMVTYGTNPGMGMSISKDIPSLESVPEAGRISYKK
SLDYMGFKAGESMLGKKVDYVFLGSCTNGRIEDFRAFASLVKGKKKADDVTVWLVPGSWQVEREIRAEGIDKVLAEAGFV
LRQPGCSACLAMNEDKVPAGKYAVSTSNRNFEGRQGPGARTILAGPLVAAAAAVTGKITDPRELM
>Mature_464_residues
SKTLFEKIWDKHVVDTLPDGTIQLYIDRLYCHEVTSPQAFAGLRARGLKPFRPAQITCMPDHNIPTLNQDKPIEDPISKN
QVDTLEENAKYFGLTYYGLQHPKNGIIHVVGPETGLTQPGMTIVCGDSHTSTHGAMGAIAFGIGTSEVEMTLATQCIMQR
KPKTMRITIDGVRKPGVTAKDFALYIISKMSTSGATGYFVEYAGEAIRNTTMEERLTICNLSIEMGARGGMIAPDDVTFD
YLKDREFAPRGEEWERKVSEWRQLYSDPDAKFDKEIVFHAEDIDPMVTYGTNPGMGMSISKDIPSLESVPEAGRISYKKS
LDYMGFKAGESMLGKKVDYVFLGSCTNGRIEDFRAFASLVKGKKKADDVTVWLVPGSWQVEREIRAEGIDKVLAEAGFVL
RQPGCSACLAMNEDKVPAGKYAVSTSNRNFEGRQGPGARTILAGPLVAAAAAVTGKITDPRELM

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI8659555, Length=469, Percent_Identity=25.1599147121535, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI4501867, Length=478, Percent_Identity=24.2677824267782, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI41352693, Length=395, Percent_Identity=24.0506329113924, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI1786259, Length=465, Percent_Identity=51.6129032258064, Blast_Score=473, Evalue=1e-134,
Organism=Escherichia coli, GI1787531, Length=368, Percent_Identity=25.8152173913043, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI2367097, Length=383, Percent_Identity=25.3263707571802, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI25149337, Length=450, Percent_Identity=26.4444444444444, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI32564738, Length=440, Percent_Identity=26.1363636363636, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17568399, Length=439, Percent_Identity=24.8291571753986, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI25149342, Length=306, Percent_Identity=26.797385620915, Blast_Score=92, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6321429, Length=470, Percent_Identity=49.5744680851064, Blast_Score=447, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6322261, Length=481, Percent_Identity=27.2349272349272, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6320440, Length=488, Percent_Identity=26.6393442622951, Blast_Score=127, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6323335, Length=458, Percent_Identity=25.1091703056769, Blast_Score=117, Evalue=3e-27,
Organism=Drosophila melanogaster, GI281365315, Length=473, Percent_Identity=25.7928118393235, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI17864292, Length=473, Percent_Identity=25.7928118393235, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI161076999, Length=438, Percent_Identity=25.3424657534247, Blast_Score=102, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24645686, Length=471, Percent_Identity=25.0530785562633, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI28571643, Length=475, Percent_Identity=24.2105263157895, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI17137564, Length=445, Percent_Identity=24.7191011235955, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 51137; Mature: 51006

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTLFEKIWDKHVVDTLPDGTIQLYIDRLYCHEVTSPQAFAGLRARGLKPFRPAQITCM
CCHHHHHHHHHHHHHHCCCCCEEEEEEEEHHHHCCCCCHHHHHHHHCCCCCCCCCEEEEE
PDHNIPTLNQDKPIEDPISKNQVDTLEENAKYFGLTYYGLQHPKNGIIHVVGPETGLTQP
CCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEEEEEEECCCCCCCEEEEECCCCCCCCC
GMTIVCGDSHTSTHGAMGAIAFGIGTSEVEMTLATQCIMQRKPKTMRITIDGVRKPGVTA
CCEEEECCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCH
KDFALYIISKMSTSGATGYFVEYAGEAIRNTTMEERLTICNLSIEMGARGGMIAPDDVTF
HHHHEEEEEHHCCCCCCEEHHHHHHHHHHHCCHHHHEEEEEEEEEECCCCCCCCCCCCCH
DYLKDREFAPRGEEWERKVSEWRQLYSDPDAKFDKEIVFHAEDIDPMVTYGTNPGMGMSI
HHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCEEEECCCCCCCCCC
SKDIPSLESVPEAGRISYKKSLDYMGFKAGESMLGKKVDYVFLGSCTNGRIEDFRAFASL
CCCCCCHHCCCCCCCEEHHHCCCCCCCCCCHHHHCCCCCEEEEECCCCCCHHHHHHHHHH
VKGKKKADDVTVWLVPGSWQVEREIRAEGIDKVLAEAGFVLRQPGCSACLAMNEDKVPAG
HCCCCCCCCEEEEEECCCEEEHHHHHHHHHHHHHHHCCCEEECCCCCEEEECCCCCCCCC
KYAVSTSNRNFEGRQGPGARTILAGPLVAAAAAVTGKITDPRELM
CEEEECCCCCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCCHHHCC
>Mature Secondary Structure 
SKTLFEKIWDKHVVDTLPDGTIQLYIDRLYCHEVTSPQAFAGLRARGLKPFRPAQITCM
CHHHHHHHHHHHHHHCCCCCEEEEEEEEHHHHCCCCCHHHHHHHHCCCCCCCCCEEEEE
PDHNIPTLNQDKPIEDPISKNQVDTLEENAKYFGLTYYGLQHPKNGIIHVVGPETGLTQP
CCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEEEEEEECCCCCCCEEEEECCCCCCCCC
GMTIVCGDSHTSTHGAMGAIAFGIGTSEVEMTLATQCIMQRKPKTMRITIDGVRKPGVTA
CCEEEECCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCH
KDFALYIISKMSTSGATGYFVEYAGEAIRNTTMEERLTICNLSIEMGARGGMIAPDDVTF
HHHHEEEEEHHCCCCCCEEHHHHHHHHHHHCCHHHHEEEEEEEEEECCCCCCCCCCCCCH
DYLKDREFAPRGEEWERKVSEWRQLYSDPDAKFDKEIVFHAEDIDPMVTYGTNPGMGMSI
HHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCEEEECCCCCCCCCC
SKDIPSLESVPEAGRISYKKSLDYMGFKAGESMLGKKVDYVFLGSCTNGRIEDFRAFASL
CCCCCCHHCCCCCCCEEHHHCCCCCCCCCCHHHHCCCCCEEEEECCCCCCHHHHHHHHHH
VKGKKKADDVTVWLVPGSWQVEREIRAEGIDKVLAEAGFVLRQPGCSACLAMNEDKVPAG
HCCCCCCCCEEEEEECCCEEEHHHHHHHHHHHHHHHCCCEEECCCCCEEEECCCCCCCCC
KYAVSTSNRNFEGRQGPGARTILAGPLVAAAAAVTGKITDPRELM
CEEEECCCCCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA