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The map label for this gene is leuA [H]

Identifier: 150008674

GI number: 150008674

Start: 2434787

End: 2436289

Strand: Reverse

Name: leuA [H]

Synonym: BDI_2064

Alternate gene names: 150008674

Gene position: 2436289-2434787 (Counterclockwise)

Preceding gene: 150008675

Following gene: 150008673

Centisome position: 50.64

GC content: 47.31

Gene sequence:

>1503_bases
ATGTCAGACAGATTATTTATTTTCGACACCACCTTACGTGATGGTGAACAGGTTCCGGGATGCCAGTTGAATACAGTGGA
AAAGATTCAAGTAGCTAAGGCGTTGGAACAGCTAGGTGTTGATGTGATCGAGGCTGGTTTCCCTGTCTCCAGTCCCGGGG
ATTTTAAGAGTGTGGTTGAGATCTCAAAGGCCGTGACTTGGCCGACCATCTGTGCCTTGACCCGTGCGGTGGAGAAAGAT
ATCGACGTGGCAGCCGAGGCGTTGAGATTCGCTAAGCGAGGACGTATTCATACGGGTATCGGTACTTCTGATTCTCATAT
CAAATACAAGTTTAACTCCACGAGAGAGGAGATTATAGAGCGTGCGATCGCCGCCACGAAATACGCTAAAAAGTATGTGG
AGGACGTAGAATTCTACTGTGAGGATGCCGGGCGTACGGATAATGAATATCTGGCCCGTGTCGTGGAGGCCGTGATCAAG
GCCGGGGCTACCGTAGTGAATATCCCGGATACGACAGGATATTGTTTGCCGGATGAGTACGGGGCGAAGATTAAGTATCT
GATGGAACATGTGGATGGCGTACATAATGCGATCTTGTCCACTCATTGCCATAATGACTTGGGTATGGCTACCGCAAATA
CCGTACAAGGCGTGTTGAACGGAGCCCGTCAGGTGGAGGTAACCATAAACGGCATCGGTGAGCGTGCGGGTAACACGTCA
TTGGAAGAAGTCGCCATGGTTTTCAAGAGCCATAAAGAACGTGATATCATCACCAATATCACGACGAATAAGATCTATAG
TACGAGCCGTATGGTTTCTAGCTTGATGAATATGCCGGTACAACCGAATAAGGCTATTGTCGGACGTAATGCGTTCGCTC
ACTCCTCCGGTATCCATCAAGATGGCGTTTTGAAGAATGCGCAAACCTACGAGATCATCGATCCGAAGGATGTGGGTATT
GATGATAATGCGATCGTATTGACTGCTCGTAGCGGTCGTGCGGCGTTGAAGCATCGCCTGCATGTTTTAGGTGTGGATCT
GGAGCAGGAGAAATTGGACAAGGTGTACGATGAGTTCTTGAAACTGGCCGATCGTAAGAAAGATATTAATGATGATGATG
TTTTGATGTTGGTCGGAAAGGATCGTACGGCCACCCACCGTATTAAATTGGAATATTTACAGGTAACTAGTGGTGTTGGA
GTACAATCAGTCGCAAGTATTTGTTTAAATTTGTCGGGAGTAAGAAGATATGAGGCCGCGGCTGGTAACGGTCCGGTTGA
CGCGGGTATCAAGGCGGTGAAAAAAGCCATTAGTAATAGTGACATGACGATTCAGGAATTCTTGATCCAAGCCATTAATA
AAGGTAGCGATGATACGGGCAAGGTGCATATGCAGGTAGAGTATAACGGGGCTACCTATTATGGGTTTTCTGCGAATACC
GATATTATCGCTGCTTCCGTGGAGGCTTTCGTGGACGCCATCAATAAGTTTATAGTAGACTAA

Upstream 100 bases:

>100_bases
TTTGCGAGCATTACGATATATGAAAGAGCCTTTCTCACTTCCTTGTGAGAGAGGCTTTTTTAGTTAGTATAGGATATTAG
TAAATTAAGAATATAAGATT

Downstream 100 bases:

>100_bases
AAATAGACAAGACAATGAGCAAGACATTATTTGAGAAAATCTGGGACAAACACGTGGTGGACACGTTGCCCGACGGAACC
ATACAATTGTATATCGACCG

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 500; Mature: 499

Protein sequence:

>500_residues
MSDRLFIFDTTLRDGEQVPGCQLNTVEKIQVAKALEQLGVDVIEAGFPVSSPGDFKSVVEISKAVTWPTICALTRAVEKD
IDVAAEALRFAKRGRIHTGIGTSDSHIKYKFNSTREEIIERAIAATKYAKKYVEDVEFYCEDAGRTDNEYLARVVEAVIK
AGATVVNIPDTTGYCLPDEYGAKIKYLMEHVDGVHNAILSTHCHNDLGMATANTVQGVLNGARQVEVTINGIGERAGNTS
LEEVAMVFKSHKERDIITNITTNKIYSTSRMVSSLMNMPVQPNKAIVGRNAFAHSSGIHQDGVLKNAQTYEIIDPKDVGI
DDNAIVLTARSGRAALKHRLHVLGVDLEQEKLDKVYDEFLKLADRKKDINDDDVLMLVGKDRTATHRIKLEYLQVTSGVG
VQSVASICLNLSGVRRYEAAAGNGPVDAGIKAVKKAISNSDMTIQEFLIQAINKGSDDTGKVHMQVEYNGATYYGFSANT
DIIAASVEAFVDAINKFIVD

Sequences:

>Translated_500_residues
MSDRLFIFDTTLRDGEQVPGCQLNTVEKIQVAKALEQLGVDVIEAGFPVSSPGDFKSVVEISKAVTWPTICALTRAVEKD
IDVAAEALRFAKRGRIHTGIGTSDSHIKYKFNSTREEIIERAIAATKYAKKYVEDVEFYCEDAGRTDNEYLARVVEAVIK
AGATVVNIPDTTGYCLPDEYGAKIKYLMEHVDGVHNAILSTHCHNDLGMATANTVQGVLNGARQVEVTINGIGERAGNTS
LEEVAMVFKSHKERDIITNITTNKIYSTSRMVSSLMNMPVQPNKAIVGRNAFAHSSGIHQDGVLKNAQTYEIIDPKDVGI
DDNAIVLTARSGRAALKHRLHVLGVDLEQEKLDKVYDEFLKLADRKKDINDDDVLMLVGKDRTATHRIKLEYLQVTSGVG
VQSVASICLNLSGVRRYEAAAGNGPVDAGIKAVKKAISNSDMTIQEFLIQAINKGSDDTGKVHMQVEYNGATYYGFSANT
DIIAASVEAFVDAINKFIVD
>Mature_499_residues
SDRLFIFDTTLRDGEQVPGCQLNTVEKIQVAKALEQLGVDVIEAGFPVSSPGDFKSVVEISKAVTWPTICALTRAVEKDI
DVAAEALRFAKRGRIHTGIGTSDSHIKYKFNSTREEIIERAIAATKYAKKYVEDVEFYCEDAGRTDNEYLARVVEAVIKA
GATVVNIPDTTGYCLPDEYGAKIKYLMEHVDGVHNAILSTHCHNDLGMATANTVQGVLNGARQVEVTINGIGERAGNTSL
EEVAMVFKSHKERDIITNITTNKIYSTSRMVSSLMNMPVQPNKAIVGRNAFAHSSGIHQDGVLKNAQTYEIIDPKDVGID
DNAIVLTARSGRAALKHRLHVLGVDLEQEKLDKVYDEFLKLADRKKDINDDDVLMLVGKDRTATHRIKLEYLQVTSGVGV
QSVASICLNLSGVRRYEAAAGNGPVDAGIKAVKKAISNSDMTIQEFLIQAINKGSDDTGKVHMQVEYNGATYYGFSANTD
IIAASVEAFVDAINKFIVD

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=498, Percent_Identity=49.5983935742972, Blast_Score=466, Evalue=1e-132,
Organism=Saccharomyces cerevisiae, GI6324682, Length=555, Percent_Identity=26.4864864864865, Blast_Score=152, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6320019, Length=380, Percent_Identity=30.5263157894737, Blast_Score=144, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6320071, Length=380, Percent_Identity=30.5263157894737, Blast_Score=144, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6324225, Length=551, Percent_Identity=27.4047186932849, Blast_Score=144, Evalue=4e-35,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 54633; Mature: 54501

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDRLFIFDTTLRDGEQVPGCQLNTVEKIQVAKALEQLGVDVIEAGFPVSSPGDFKSVVE
CCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCHHHHHH
ISKAVTWPTICALTRAVEKDIDVAAEALRFAKRGRIHTGIGTSDSHIKYKFNSTREEIIE
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCEEEEEECCHHHHHHH
RAIAATKYAKKYVEDVEFYCEDAGRTDNEYLARVVEAVIKAGATVVNIPDTTGYCLPDEY
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCHH
GAKIKYLMEHVDGVHNAILSTHCHNDLGMATANTVQGVLNGARQVEVTINGIGERAGNTS
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCEEEEEEEEECCCCCCCCC
LEEVAMVFKSHKERDIITNITTNKIYSTSRMVSSLMNMPVQPNKAIVGRNAFAHSSGIHQ
HHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHHCCCCCCCCEEECCCCHHCCCCCCC
DGVLKNAQTYEIIDPKDVGIDDNAIVLTARSGRAALKHRLHVLGVDLEQEKLDKVYDEFL
CCCCCCCCEEEEECCCCCCCCCCEEEEEECCCHHHHHHHHHHEECCCCHHHHHHHHHHHH
KLADRKKDINDDDVLMLVGKDRTATHRIKLEYLQVTSGVGVQSVASICLNLSGVRRYEAA
HHHHHCCCCCCCCEEEEEECCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHC
AGNGPVDAGIKAVKKAISNSDMTIQEFLIQAINKGSDDTGKVHMQVEYNGATYYGFSANT
CCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEECCEEEEECCCCC
DIIAASVEAFVDAINKFIVD
CHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SDRLFIFDTTLRDGEQVPGCQLNTVEKIQVAKALEQLGVDVIEAGFPVSSPGDFKSVVE
CCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCHHHHHH
ISKAVTWPTICALTRAVEKDIDVAAEALRFAKRGRIHTGIGTSDSHIKYKFNSTREEIIE
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCEEEEEECCHHHHHHH
RAIAATKYAKKYVEDVEFYCEDAGRTDNEYLARVVEAVIKAGATVVNIPDTTGYCLPDEY
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCHH
GAKIKYLMEHVDGVHNAILSTHCHNDLGMATANTVQGVLNGARQVEVTINGIGERAGNTS
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCEEEEEEEEECCCCCCCCC
LEEVAMVFKSHKERDIITNITTNKIYSTSRMVSSLMNMPVQPNKAIVGRNAFAHSSGIHQ
HHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHHCCCCCCCCEEECCCCHHCCCCCCC
DGVLKNAQTYEIIDPKDVGIDDNAIVLTARSGRAALKHRLHVLGVDLEQEKLDKVYDEFL
CCCCCCCCEEEEECCCCCCCCCCEEEEEECCCHHHHHHHHHHEECCCCHHHHHHHHHHHH
KLADRKKDINDDDVLMLVGKDRTATHRIKLEYLQVTSGVGVQSVASICLNLSGVRRYEAA
HHHHHCCCCCCCCEEEEEECCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHC
AGNGPVDAGIKAVKKAISNSDMTIQEFLIQAINKGSDDTGKVHMQVEYNGATYYGFSANT
CCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEECCEEEEECCCCC
DIIAASVEAFVDAINKFIVD
CHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA