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The map label for this gene is nfsA [C]

Identifier: 150008354

GI number: 150008354

Start: 2044399

End: 2044983

Strand: Direct

Name: nfsA [C]

Synonym: BDI_1728

Alternate gene names: 150008354

Gene position: 2044399-2044983 (Clockwise)

Preceding gene: 150008353

Following gene: 150008359

Centisome position: 42.49

GC content: 52.65

Gene sequence:

>585_bases
ATGAGAATCTGTCTTTTCTTCTTGATCACCGTTTTCCTCATGGCCTGCTCCTCTACGGGGAACCAGCGGCAGGAACCGGA
AAACCAGACCTTGGAAACGATACTCAATCGCAAAAGCGTACGTAAGTATAAGGATCGCCCGGTAGAAAAGGAAAAGATAG
ACAAACTGATACGAGCCGGAATGGCCGCCCCCTCCTCCCGTGACAGGCGACCTTGGGAATTCATTATCGTAACCGACCGG
AAAGCCTTGGACACAATGGCCGAAGGTCTCCCCTTCGCCCGGATGCTGAAAGAGACAAGGCAAGCGATCGTCGTTTGCGG
CGATACGATCAAATCCTCCAACGCTTGGTTTCTGGATTGTTCCGCCGCCTCGCAAAACCTGCTACTTGCCGCCGAATCCA
TGGGATTGGGAGCCGTATGGACGGCTGTTTACCCCTATCCGGACCGGATCGAGATCGTCCGGAAAGAGCTTCGGCTACCG
GATCATATCATGCCGTTGAACGTCATACCTGTGGGATACCCCATGCAGAAAGAGACTCCCAAAAACAAATACAACGTCCA
ACAGATCCATCACAACGGCTGGTAA

Upstream 100 bases:

>100_bases
GGAACGATGTCTCCGAGCACCGGAAAGAAGCCTTGCAGAAGATGCCTTCTACGTTGATATTAAGTAAATAATCCGTTATA
AACCATATAAATCCGTCTTT

Downstream 100 bases:

>100_bases
AGCTATATGACTTCCAGATAACTCCGTCCGTTTCCCGCCCGGTTCACCCGGATCTGCACGGGAATACGTTCCGTCATTTC
CTGTACGTGCGAGATAACGC

Product: putative NADH dehydrogenase/NAD(P)H nitroreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 194; Mature: 194

Protein sequence:

>194_residues
MRICLFFLITVFLMACSSTGNQRQEPENQTLETILNRKSVRKYKDRPVEKEKIDKLIRAGMAAPSSRDRRPWEFIIVTDR
KALDTMAEGLPFARMLKETRQAIVVCGDTIKSSNAWFLDCSAASQNLLLAAESMGLGAVWTAVYPYPDRIEIVRKELRLP
DHIMPLNVIPVGYPMQKETPKNKYNVQQIHHNGW

Sequences:

>Translated_194_residues
MRICLFFLITVFLMACSSTGNQRQEPENQTLETILNRKSVRKYKDRPVEKEKIDKLIRAGMAAPSSRDRRPWEFIIVTDR
KALDTMAEGLPFARMLKETRQAIVVCGDTIKSSNAWFLDCSAASQNLLLAAESMGLGAVWTAVYPYPDRIEIVRKELRLP
DHIMPLNVIPVGYPMQKETPKNKYNVQQIHHNGW
>Mature_194_residues
MRICLFFLITVFLMACSSTGNQRQEPENQTLETILNRKSVRKYKDRPVEKEKIDKLIRAGMAAPSSRDRRPWEFIIVTDR
KALDTMAEGLPFARMLKETRQAIVVCGDTIKSSNAWFLDCSAASQNLLLAAESMGLGAVWTAVYPYPDRIEIVRKELRLP
DHIMPLNVIPVGYPMQKETPKNKYNVQQIHHNGW

Specific function: Reduction Of Nitroaromatic Compounds Using NADH. Reduces Nitrofurazone By A Ping-Pong Bi-Bi Mechanism Possibly To Generate A Two-Electron Transfer Product. Major Component Of The Oxygen- Insensitive Nitroreductase Activity In E.Coli. [C]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787075, Length=159, Percent_Identity=28.9308176100629, Blast_Score=60, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 22249; Mature: 22249

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRICLFFLITVFLMACSSTGNQRQEPENQTLETILNRKSVRKYKDRPVEKEKIDKLIRAG
CHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHC
MAAPSSRDRRPWEFIIVTDRKALDTMAEGLPFARMLKETRQAIVVCGDTIKSSNAWFLDC
CCCCCCCCCCCEEEEEEECCHHHHHHHCCCCHHHHHHHHHHEEEEECCCCCCCCCEEEEC
SAASQNLLLAAESMGLGAVWTAVYPYPDRIEIVRKELRLPDHIMPLNVIPVGYPMQKETP
CCCCCCEEEEECCCCCCEEEHEECCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCC
KNKYNVQQIHHNGW
CCCCCHHHHCCCCC
>Mature Secondary Structure
MRICLFFLITVFLMACSSTGNQRQEPENQTLETILNRKSVRKYKDRPVEKEKIDKLIRAG
CHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHC
MAAPSSRDRRPWEFIIVTDRKALDTMAEGLPFARMLKETRQAIVVCGDTIKSSNAWFLDC
CCCCCCCCCCCEEEEEEECCHHHHHHHCCCCHHHHHHHHHHEEEEECCCCCCCCCEEEEC
SAASQNLLLAAESMGLGAVWTAVYPYPDRIEIVRKELRLPDHIMPLNVIPVGYPMQKETP
CCCCCCEEEEECCCCCCEEEHEECCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCC
KNKYNVQQIHHNGW
CCCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]