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The map label for this gene is tilS

Identifier: 150008036

GI number: 150008036

Start: 1626571

End: 1627887

Strand: Reverse

Name: tilS

Synonym: BDI_1399

Alternate gene names: 150008036

Gene position: 1627887-1626571 (Counterclockwise)

Preceding gene: 150008037

Following gene: 150008035

Centisome position: 33.83

GC content: 46.77

Gene sequence:

>1317_bases
ATGATACATACGATACGTTTATATATAGAGAAGTACCGGCTGTTATCCGAGGATCGTCCGGTTTTGGTCGGATTGAGCGG
TGGTGCCGATTCTGTGGCTTTGTTGGGCGTATTGGTGCGTTTGGGATATCCGTGTATAGCGCTTCATTGTAACTTTCATT
TGCGTGGGGAGGAGTCGGATCGGGATGAGGCATTTGCCTGTGAGTTCGCGGAATCTTTGGAAGTACCCTTTCATAAGATA
GATTTCGATACGATCTCTTATGCCGGAGAGAGGCATCTCTCGATAGAGATGGCTGCCCGTGAATTGCGCTATGCTTGGTT
TGAGGAGATGCGGGAGCGGCTGGGAGGGCAGGCGACCGCCGTCGCCCATCACCGGGACGATAATGTGGAGACGGTCTTGA
TGAATTTGATACGGGGCACGGGAATCCGGGGGATGAGCGGGATCCGTCCGAGGAATGGTTTCATCGTCCGTCCTTTGCTG
TGCGTAAGCCGTGAGGATATCTTGGCTTGGCTGGCGGATCAAGGCTACGCCTATATGGTGGATAGCACGAATTTATCGGA
TGCTTATACCCGTAATTTCATCCGTTTGAATGTGTTGCCGCTTTTGGAGGAGATAAATCCATCGGCCCGGAATACCATCG
CACGTTCCGCCGAGCATCTTTCTGCCGCAGAGACTATATATATATATGTATTGGAACAAGCCCGGAAAGAGGTTGTCGTA
TCGGATGATCGTCTTTCTATCGGGGCGTTGATGCGGTTTCCGGCTCCGGAGACGATTTTGTATGAGTTATTAAAAGAATA
TGGTTTTACCCGTCTGGTCTCGGACGATATTTTCGCTGCCTTGACCAAGGAGCCTGGTAAGCTGTTTTACTCTTCCACCC
ATCGTTTGCTGAAGGATCGGGATTATTTATGGATCACTTCTTTAGAGAAGAAGGAAAAGCGAACCTTCGTTTTAGATCCA
GAAAAGGGCATTAATCATGAACCTATTGAACTATCTTTCCAAAAACTTGTTATAACTATAGATTTCCCTATCGAGAAGAA
TAAGCGGATCGCTTATTTGGATTATGATAAACTCGATTTCCCTTTAACATTGCGGACTTGGAAAGAAGGCGATTGGTTTA
TTCCCTTCGGAATGAAAGGACGTAAGAAGTTGAGTGACTATTTCTCCGATCATAAATTTAGCCGGATTGAGAAGGAGCGC
ACATGGCTTCTTTGTAGTGGCGACGCTATCGTTTGGGTGGTAGGAGAAAGGACTGATAATCGTTTTCGTATAGACGAATC
AACAAAGCGTGTGCTAATTGTGAATTTTTTGGGCTAA

Upstream 100 bases:

>100_bases
CCGGAATTAGCAGGTGTAGAAGATTAATTCAAAAGATCTATAAGTAATGGCTCTAGCTAAAACCGGCTAGGGCCATTATT
GTTTTTAATACAAAGATGTT

Downstream 100 bases:

>100_bases
ATCCTCGTTTATTGTAAAAATATTCTTACATTTGCGAAGATTTACTCCGCAGATATGTTACTAATGGTTCAAATTAGCTG
AACAATCCGGTTATTACATG

Product: putative cell-cycle protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MIHTIRLYIEKYRLLSEDRPVLVGLSGGADSVALLGVLVRLGYPCIALHCNFHLRGEESDRDEAFACEFAESLEVPFHKI
DFDTISYAGERHLSIEMAARELRYAWFEEMRERLGGQATAVAHHRDDNVETVLMNLIRGTGIRGMSGIRPRNGFIVRPLL
CVSREDILAWLADQGYAYMVDSTNLSDAYTRNFIRLNVLPLLEEINPSARNTIARSAEHLSAAETIYIYVLEQARKEVVV
SDDRLSIGALMRFPAPETILYELLKEYGFTRLVSDDIFAALTKEPGKLFYSSTHRLLKDRDYLWITSLEKKEKRTFVLDP
EKGINHEPIELSFQKLVITIDFPIEKNKRIAYLDYDKLDFPLTLRTWKEGDWFIPFGMKGRKKLSDYFSDHKFSRIEKER
TWLLCSGDAIVWVVGERTDNRFRIDESTKRVLIVNFLG

Sequences:

>Translated_438_residues
MIHTIRLYIEKYRLLSEDRPVLVGLSGGADSVALLGVLVRLGYPCIALHCNFHLRGEESDRDEAFACEFAESLEVPFHKI
DFDTISYAGERHLSIEMAARELRYAWFEEMRERLGGQATAVAHHRDDNVETVLMNLIRGTGIRGMSGIRPRNGFIVRPLL
CVSREDILAWLADQGYAYMVDSTNLSDAYTRNFIRLNVLPLLEEINPSARNTIARSAEHLSAAETIYIYVLEQARKEVVV
SDDRLSIGALMRFPAPETILYELLKEYGFTRLVSDDIFAALTKEPGKLFYSSTHRLLKDRDYLWITSLEKKEKRTFVLDP
EKGINHEPIELSFQKLVITIDFPIEKNKRIAYLDYDKLDFPLTLRTWKEGDWFIPFGMKGRKKLSDYFSDHKFSRIEKER
TWLLCSGDAIVWVVGERTDNRFRIDESTKRVLIVNFLG
>Mature_438_residues
MIHTIRLYIEKYRLLSEDRPVLVGLSGGADSVALLGVLVRLGYPCIALHCNFHLRGEESDRDEAFACEFAESLEVPFHKI
DFDTISYAGERHLSIEMAARELRYAWFEEMRERLGGQATAVAHHRDDNVETVLMNLIRGTGIRGMSGIRPRNGFIVRPLL
CVSREDILAWLADQGYAYMVDSTNLSDAYTRNFIRLNVLPLLEEINPSARNTIARSAEHLSAAETIYIYVLEQARKEVVV
SDDRLSIGALMRFPAPETILYELLKEYGFTRLVSDDIFAALTKEPGKLFYSSTHRLLKDRDYLWITSLEKKEKRTFVLDP
EKGINHEPIELSFQKLVITIDFPIEKNKRIAYLDYDKLDFPLTLRTWKEGDWFIPFGMKGRKKLSDYFSDHKFSRIEKER
TWLLCSGDAIVWVVGERTDNRFRIDESTKRVLIVNFLG

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family

Homologues:

Organism=Escherichia coli, GI1786386, Length=222, Percent_Identity=34.6846846846847, Blast_Score=98, Evalue=9e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TILS_PARD8 (A6LBU3)

Other databases:

- EMBL:   CP000140
- RefSeq:   YP_001302779.1
- ProteinModelPortal:   A6LBU3
- SMR:   A6LBU3
- STRING:   A6LBU3
- GeneID:   5306552
- GenomeReviews:   CP000140_GR
- KEGG:   pdi:BDI_1399
- eggNOG:   COG0037
- HOGENOM:   HBG668702
- OMA:   ISIEMAA
- ProtClustDB:   CLSK2391376
- BioCyc:   PDIS435591:BDI_1399-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01161
- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11807:SF2
- SMART:   SM00977
- TIGRFAMs:   TIGR02433
- TIGRFAMs:   TIGR02432

Pfam domain/function: PF01171 ATP_bind_3; PF11734 TilS_C; SSF56037 B3_4

EC number: NA

Molecular weight: Translated: 50589; Mature: 50589

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHTIRLYIEKYRLLSEDRPVLVGLSGGADSVALLGVLVRLGYPCIALHCNFHLRGEESD
CEEEEEEHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEEEEEEEECCCCC
RDEAFACEFAESLEVPFHKIDFDTISYAGERHLSIEMAARELRYAWFEEMRERLGGQATA
CCHHHHHHHHHHCCCCEEEECCHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCEEE
VAHHRDDNVETVLMNLIRGTGIRGMSGIRPRNGFIVRPLLCVSREDILAWLADQGYAYMV
EEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEHHHHCCHHHHHHHHHCCCEEEEE
DSTNLSDAYTRNFIRLNVLPLLEEINPSARNTIARSAEHLSAAETIYIYVLEQARKEVVV
ECCCCCHHHHCCEEEEEHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEEEEHHCCCEEE
SDDRLSIGALMRFPAPETILYELLKEYGFTRLVSDDIFAALTKEPGKLFYSSTHRLLKDR
ECCCEEEEHEEECCCCHHHHHHHHHHCCCEEEECHHHHHHHCCCCCHHHHHHHHHHHHCC
DYLWITSLEKKEKRTFVLDPEKGINHEPIELSFQKLVITIDFPIEKNKRIAYLDYDKLDF
CEEEEECCCCCCCCEEEECCCCCCCCCCCEEEEEEEEEEEECCCCCCCEEEEEECCCCCC
PLTLRTWKEGDWFIPFGMKGRKKLSDYFSDHKFSRIEKERTWLLCSGDAIVWVVGERTDN
CEEEEEECCCCEEEECCCCCHHHHHHHHHCCHHHHHHCCCEEEEECCCEEEEEEECCCCC
RFRIDESTKRVLIVNFLG
EEEECCCCCEEEEEEECC
>Mature Secondary Structure
MIHTIRLYIEKYRLLSEDRPVLVGLSGGADSVALLGVLVRLGYPCIALHCNFHLRGEESD
CEEEEEEHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEEEEEEEECCCCC
RDEAFACEFAESLEVPFHKIDFDTISYAGERHLSIEMAARELRYAWFEEMRERLGGQATA
CCHHHHHHHHHHCCCCEEEECCHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCEEE
VAHHRDDNVETVLMNLIRGTGIRGMSGIRPRNGFIVRPLLCVSREDILAWLADQGYAYMV
EEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEHHHHCCHHHHHHHHHCCCEEEEE
DSTNLSDAYTRNFIRLNVLPLLEEINPSARNTIARSAEHLSAAETIYIYVLEQARKEVVV
ECCCCCHHHHCCEEEEEHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEEEEHHCCCEEE
SDDRLSIGALMRFPAPETILYELLKEYGFTRLVSDDIFAALTKEPGKLFYSSTHRLLKDR
ECCCEEEEHEEECCCCHHHHHHHHHHCCCEEEECHHHHHHHCCCCCHHHHHHHHHHHHCC
DYLWITSLEKKEKRTFVLDPEKGINHEPIELSFQKLVITIDFPIEKNKRIAYLDYDKLDF
CEEEEECCCCCCCCEEEECCCCCCCCCCCEEEEEEEEEEEECCCCCCCEEEEEECCCCCC
PLTLRTWKEGDWFIPFGMKGRKKLSDYFSDHKFSRIEKERTWLLCSGDAIVWVVGERTDN
CEEEEEECCCCEEEECCCCCHHHHHHHHHCCHHHHHHCCCEEEEECCCEEEEEEECCCCC
RFRIDESTKRVLIVNFLG
EEEECCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA