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The map label for this gene is gacS [H]

Identifier: 150008002

GI number: 150008002

Start: 1583127

End: 1584029

Strand: Reverse

Name: gacS [H]

Synonym: BDI_1364

Alternate gene names: 150008002

Gene position: 1584029-1583127 (Counterclockwise)

Preceding gene: 150008008

Following gene: 150008001

Centisome position: 32.92

GC content: 44.19

Gene sequence:

>903_bases
ATGGTAAAGGAAATGGTCGAGTATGAGAAACCGAATGGAGAGGTGAGAATCGTAAATATCGTACGTTTACTGATCACGAA
TCCGGACCCGGGAGCCTCGCCTCTGTTATTGGTCATGCTGAATGATATCACGGAGCAGCGTCAGGACCAGTTGGCGCTGA
TGAAAGTGCAAGAGGCGGATAAGATGAAAAACGCTTTTATCGCTAATATGAGCCATGAGATCCGGACTCCGCTGAATGCT
ATCGTTGGCTTTGCCCATCTCGTGACGGAAACGACTAATGCCAGTGAGCAAAGGGAATACTTTTCGATTATAAATAAGAA
TTGCGATTTATTGTTGAAACTGATTAACGATATCTTGGATTTGTCGAAGATCGAGTCCGGTCGGTTAAACTATAACGTGT
CGGAAGTTGAGTTGAGGGATATTTGCCAAGAGGCCTATGTGGTACAATCCTTGAAGATGACTTCCGATGTGGCATTATTA
TATAACTCGGTCGCCATGCCTTCTGTTCGTCTGTGGATCGATCCGCACCGGGTAGAACAGGTCTTGTTAAATCTCTTGTC
TAATGCCATTAAGTTTACTTCCAAAGGTTTTATCTCGCTTTTTTATGAGGTGGAAGATATGTTCGTACGTGTCTCTGTCA
TGGATACCGGAATCGGGATATCAGAGGAAAAGCTGGAGTCTGTTTTCGAGCGTTTCGTGAAATTGGATGATTTTTATCAA
GGGGCTGGTTTGGGACTACCGATCTGCAAGATGATTGTAGAGCAGTTGGGTGGTGAGATCGGTGTGCGATCTGAGCTTGG
CAAAGGTTCCACTTTCTGGTTCACCTTACCATTGGTTGTTACGGATAAGGCTGTTGTGGAGAAGGATGATCCTTCTGTTT
TAAGTGAATGTCAACTATATTAA

Upstream 100 bases:

>100_bases
GGAAGATATCTTAGGGAAGACGGATTTCGAGCTTTTCCCTGATTCCCGGCGGGCGCATGAGATCTGAACGGAAGATTACC
ATACGATCAAGACGGGACGT

Downstream 100 bases:

>100_bases
ATAAAAAAGAAATGAAAAATGCGGTTGTCTTTTTAGCGACAGGCTTTGAGGAAATAGAGGCCTTGGGTACGATTGATGTC
TTGAGGCGTGGTGGTGTACG

Product: two-component system sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MVKEMVEYEKPNGEVRIVNIVRLLITNPDPGASPLLLVMLNDITEQRQDQLALMKVQEADKMKNAFIANMSHEIRTPLNA
IVGFAHLVTETTNASEQREYFSIINKNCDLLLKLINDILDLSKIESGRLNYNVSEVELRDICQEAYVVQSLKMTSDVALL
YNSVAMPSVRLWIDPHRVEQVLLNLLSNAIKFTSKGFISLFYEVEDMFVRVSVMDTGIGISEEKLESVFERFVKLDDFYQ
GAGLGLPICKMIVEQLGGEIGVRSELGKGSTFWFTLPLVVTDKAVVEKDDPSVLSECQLY

Sequences:

>Translated_300_residues
MVKEMVEYEKPNGEVRIVNIVRLLITNPDPGASPLLLVMLNDITEQRQDQLALMKVQEADKMKNAFIANMSHEIRTPLNA
IVGFAHLVTETTNASEQREYFSIINKNCDLLLKLINDILDLSKIESGRLNYNVSEVELRDICQEAYVVQSLKMTSDVALL
YNSVAMPSVRLWIDPHRVEQVLLNLLSNAIKFTSKGFISLFYEVEDMFVRVSVMDTGIGISEEKLESVFERFVKLDDFYQ
GAGLGLPICKMIVEQLGGEIGVRSELGKGSTFWFTLPLVVTDKAVVEKDDPSVLSECQLY
>Mature_300_residues
MVKEMVEYEKPNGEVRIVNIVRLLITNPDPGASPLLLVMLNDITEQRQDQLALMKVQEADKMKNAFIANMSHEIRTPLNA
IVGFAHLVTETTNASEQREYFSIINKNCDLLLKLINDILDLSKIESGRLNYNVSEVELRDICQEAYVVQSLKMTSDVALL
YNSVAMPSVRLWIDPHRVEQVLLNLLSNAIKFTSKGFISLFYEVEDMFVRVSVMDTGIGISEEKLESVFERFVKLDDFYQ
GAGLGLPICKMIVEQLGGEIGVRSELGKGSTFWFTLPLVVTDKAVVEKDDPSVLSECQLY

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=261, Percent_Identity=35.632183908046, Blast_Score=154, Evalue=7e-39,
Organism=Escherichia coli, GI1788713, Length=307, Percent_Identity=32.8990228013029, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI87081816, Length=247, Percent_Identity=37.246963562753, Blast_Score=137, Evalue=7e-34,
Organism=Escherichia coli, GI48994928, Length=264, Percent_Identity=33.3333333333333, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI145693157, Length=229, Percent_Identity=35.3711790393013, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1790436, Length=238, Percent_Identity=33.1932773109244, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1786912, Length=225, Percent_Identity=29.3333333333333, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1788549, Length=248, Percent_Identity=27.4193548387097, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788393, Length=244, Percent_Identity=28.6885245901639, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1786600, Length=288, Percent_Identity=26.7361111111111, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI1786783, Length=239, Percent_Identity=28.0334728033473, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI87082128, Length=226, Percent_Identity=26.1061946902655, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1790861, Length=202, Percent_Identity=27.2277227722772, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1790300, Length=245, Percent_Identity=25.3061224489796, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787894, Length=222, Percent_Identity=27.9279279279279, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 33832; Mature: 33832

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKEMVEYEKPNGEVRIVNIVRLLITNPDPGASPLLLVMLNDITEQRQDQLALMKVQEAD
CCCHHHHHCCCCCCEEEEEEEEHHEECCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHH
KMKNAFIANMSHEIRTPLNAIVGFAHLVTETTNASEQREYFSIINKNCDLLLKLINDILD
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHH
LSKIESGRLNYNVSEVELRDICQEAYVVQSLKMTSDVALLYNSVAMPSVRLWIDPHRVEQ
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHH
VLLNLLSNAIKFTSKGFISLFYEVEDMFVRVSVMDTGIGISEEKLESVFERFVKLDDFYQ
HHHHHHHHHHHHHCCHHHHHHHHHHHHEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHC
GAGLGLPICKMIVEQLGGEIGVRSELGKGSTFWFTLPLVVTDKAVVEKDDPSVLSECQLY
CCCCCHHHHHHHHHHCCCCCCCHHCCCCCCEEEEEEEEEEECCHHCCCCCCHHHHHCCCC
>Mature Secondary Structure
MVKEMVEYEKPNGEVRIVNIVRLLITNPDPGASPLLLVMLNDITEQRQDQLALMKVQEAD
CCCHHHHHCCCCCCEEEEEEEEHHEECCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHH
KMKNAFIANMSHEIRTPLNAIVGFAHLVTETTNASEQREYFSIINKNCDLLLKLINDILD
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHH
LSKIESGRLNYNVSEVELRDICQEAYVVQSLKMTSDVALLYNSVAMPSVRLWIDPHRVEQ
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHH
VLLNLLSNAIKFTSKGFISLFYEVEDMFVRVSVMDTGIGISEEKLESVFERFVKLDDFYQ
HHHHHHHHHHHHHCCHHHHHHHHHHHHEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHC
GAGLGLPICKMIVEQLGGEIGVRSELGKGSTFWFTLPLVVTDKAVVEKDDPSVLSECQLY
CCCCCHHHHHHHHHHCCCCCCCHHCCCCCCEEEEEEEEEEECCHHCCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]