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The map label for this gene is gacS [H]

Identifier: 150008008

GI number: 150008008

Start: 1590511

End: 1592037

Strand: Reverse

Name: gacS [H]

Synonym: BDI_1371

Alternate gene names: 150008008

Gene position: 1592037-1590511 (Counterclockwise)

Preceding gene: 150008011

Following gene: 150008002

Centisome position: 33.09

GC content: 46.23

Gene sequence:

>1527_bases
TTGATTAAAAAAATCAACATGGAAGTTAATACTACGGAGAACAAAACTTTGGAACAGGATTATAGGGATTTAGCAGGATT
GTTATCTAATATCGTGGACTCGTTGCCCATCTACTTTTTCGTGAAGGATACGGGTGATAATTTCAGGTACGTGTACTCCA
GCCCTTTGATGAACCAACTTTACGGACGATATCATAATGATGTGGTCGGGAAAACAGATTTTGACCTATTTCTAGATCCG
GTCGTCGCCCAATCTTTCAGGGATATGGACGAGGAGGTGGTGAAAACGGGTAAGATGCAGCGTTTCGTGGAGCAAATGAT
CGATCCGAAAGGTATCTTGCGTACTATGGATACGATGAAACTGCTAGCGCCCCGTGAGGATAAGCCTCCTTATTTAGTAG
GGATTTCATGGGATATTACAAAGCAAAGGCAGGTCGAGGAGGAGTTGCATAGCTATAATAAGCGCTTGGCGCTCGCTTGT
CAAGCGGGTAAGATCTACCCGTGGCTTTGGGATTTGGTAAACGGTACGGCGGAGCTTTCCTTGGAAGAAAACGGCCAGAT
CAAGCATGTGCAGATCACCCACGCTAGTTTTACGGAGAAGATCCATCCGGATTACCGGAAGGTATACGAGAATATTACAA
CCGCTTTCATGCGGGGCGAGATCGATTCCATGCGATTCAGTTTCCCTTGTCGTTATTTCTCGGATGAGTATGTATGGTTG
GAGAAGATCGGTGAGGTCTATGAGGCCGATCCGGATGGCAAGCCGATCCGCTCGATCGGAATCCTTCGGGATATGACCGT
CGATAAACGGCACGAGGCGGATGTGCAGGCTAAGCGTTTGGCGGAAGAGAGCGACCGGATGAAGTCCGCTTTTATCGCTA
ACATGAGCCATGAGATACGTACCCCTTTAAATGCCATCGTCGGCTTTTCCACCTTATTGGCGGAGAGTGACGATGAGGAG
GAAAAAAAGGAATATCTGAGGATCATAGAGAGTAGCAATGAGTTTTTGTTGCAGTTGATCGGTGATATCTTGGATATCTC
GAAGATCGAGTCCGGAAAGATGGAGTTTATTTATACGACCCTGCGTTTGAGTGAGATATTCGCTCCCCAGCAGCAAGCTT
TCGCCTTGAGAGTGGCCCCGGGTGTAAAGGTTATCTTTGAGTCGGACGGTAATGACTATTGTATTGTGTCGGAGAAGACC
CGTTTGACTCAGGTTTTGACCAATTTCCTTTCCAACGCCGTGAAGTTCACCAGCTCGGGTTCTATACGGTTTGGTTATCG
TTTGACCGATGATGGTATCTATGTCTACGTAACGGATACGGGTATGGGAATCAGTAAGGAGTCTCAAGCGAGCATATTCA
ATCGTTTCGTGAAATTGAACAGCTTTAAGCAAGGAACAGGATTAGGTCTTTCTATCTGCAAGACGATTATTGAGAAGTTG
AAAGGTAAAATCGGGGTTGAGTCGGAGGAGGGAAAGGGCTCTACATTCTGGTTTACGATACCTTGCCAACCGATGAAAGT
GAAATAA

Upstream 100 bases:

>100_bases
GGTTTTGCTTATATTTTCGGTCGGGATGCTCACTTCTTGGGCTTATTATTTGTATCTTTCATTAATTTTCAAGAATTTTA
CGGCCGGAATATTCAAGCCT

Downstream 100 bases:

>100_bases
GACATTCAAGTTTGGTTGGAATTTATAGGTAAATCACCTCATCCGCCAACAACCGAATCTCATCCGGATTATGTGACGTG
AAAAGAATGATCCGTTCTTC

Product: two-component system sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 508; Mature: 508

Protein sequence:

>508_residues
MIKKINMEVNTTENKTLEQDYRDLAGLLSNIVDSLPIYFFVKDTGDNFRYVYSSPLMNQLYGRYHNDVVGKTDFDLFLDP
VVAQSFRDMDEEVVKTGKMQRFVEQMIDPKGILRTMDTMKLLAPREDKPPYLVGISWDITKQRQVEEELHSYNKRLALAC
QAGKIYPWLWDLVNGTAELSLEENGQIKHVQITHASFTEKIHPDYRKVYENITTAFMRGEIDSMRFSFPCRYFSDEYVWL
EKIGEVYEADPDGKPIRSIGILRDMTVDKRHEADVQAKRLAEESDRMKSAFIANMSHEIRTPLNAIVGFSTLLAESDDEE
EKKEYLRIIESSNEFLLQLIGDILDISKIESGKMEFIYTTLRLSEIFAPQQQAFALRVAPGVKVIFESDGNDYCIVSEKT
RLTQVLTNFLSNAVKFTSSGSIRFGYRLTDDGIYVYVTDTGMGISKESQASIFNRFVKLNSFKQGTGLGLSICKTIIEKL
KGKIGVESEEGKGSTFWFTIPCQPMKVK

Sequences:

>Translated_508_residues
MIKKINMEVNTTENKTLEQDYRDLAGLLSNIVDSLPIYFFVKDTGDNFRYVYSSPLMNQLYGRYHNDVVGKTDFDLFLDP
VVAQSFRDMDEEVVKTGKMQRFVEQMIDPKGILRTMDTMKLLAPREDKPPYLVGISWDITKQRQVEEELHSYNKRLALAC
QAGKIYPWLWDLVNGTAELSLEENGQIKHVQITHASFTEKIHPDYRKVYENITTAFMRGEIDSMRFSFPCRYFSDEYVWL
EKIGEVYEADPDGKPIRSIGILRDMTVDKRHEADVQAKRLAEESDRMKSAFIANMSHEIRTPLNAIVGFSTLLAESDDEE
EKKEYLRIIESSNEFLLQLIGDILDISKIESGKMEFIYTTLRLSEIFAPQQQAFALRVAPGVKVIFESDGNDYCIVSEKT
RLTQVLTNFLSNAVKFTSSGSIRFGYRLTDDGIYVYVTDTGMGISKESQASIFNRFVKLNSFKQGTGLGLSICKTIIEKL
KGKIGVESEEGKGSTFWFTIPCQPMKVK
>Mature_508_residues
MIKKINMEVNTTENKTLEQDYRDLAGLLSNIVDSLPIYFFVKDTGDNFRYVYSSPLMNQLYGRYHNDVVGKTDFDLFLDP
VVAQSFRDMDEEVVKTGKMQRFVEQMIDPKGILRTMDTMKLLAPREDKPPYLVGISWDITKQRQVEEELHSYNKRLALAC
QAGKIYPWLWDLVNGTAELSLEENGQIKHVQITHASFTEKIHPDYRKVYENITTAFMRGEIDSMRFSFPCRYFSDEYVWL
EKIGEVYEADPDGKPIRSIGILRDMTVDKRHEADVQAKRLAEESDRMKSAFIANMSHEIRTPLNAIVGFSTLLAESDDEE
EKKEYLRIIESSNEFLLQLIGDILDISKIESGKMEFIYTTLRLSEIFAPQQQAFALRVAPGVKVIFESDGNDYCIVSEKT
RLTQVLTNFLSNAVKFTSSGSIRFGYRLTDDGIYVYVTDTGMGISKESQASIFNRFVKLNSFKQGTGLGLSICKTIIEKL
KGKIGVESEEGKGSTFWFTIPCQPMKVK

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=240, Percent_Identity=37.0833333333333, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1788713, Length=236, Percent_Identity=35.1694915254237, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI145693157, Length=260, Percent_Identity=33.8461538461538, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI87081816, Length=252, Percent_Identity=33.3333333333333, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI48994928, Length=264, Percent_Identity=32.5757575757576, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1788549, Length=286, Percent_Identity=29.3706293706294, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI1786912, Length=237, Percent_Identity=26.1603375527426, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1786600, Length=230, Percent_Identity=26.9565217391304, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1790436, Length=215, Percent_Identity=28.3720930232558, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1788393, Length=229, Percent_Identity=26.6375545851528, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1786783, Length=222, Percent_Identity=27.4774774774775, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI87082128, Length=229, Percent_Identity=28.3842794759825, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1790346, Length=219, Percent_Identity=26.027397260274, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790861, Length=222, Percent_Identity=23.8738738738739, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 58176; Mature: 58176

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS50112 PAS ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKINMEVNTTENKTLEQDYRDLAGLLSNIVDSLPIYFFVKDTGDNFRYVYSSPLMNQL
CCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEEECCHHHHHH
YGRYHNDVVGKTDFDLFLDPVVAQSFRDMDEEVVKTGKMQRFVEQMIDPKGILRTMDTMK
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LLAPREDKPPYLVGISWDITKQRQVEEELHSYNKRLALACQAGKIYPWLWDLVNGTAELS
HHCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHCCCEEEE
LEENGQIKHVQITHASFTEKIHPDYRKVYENITTAFMRGEIDSMRFSFPCRYFSDEYVWL
ECCCCCEEEEEEECHHHHHHCCHHHHHHHHHHHHHHHHCCHHCEEEECCCCCCCCCCHHH
EKIGEVYEADPDGKPIRSIGILRDMTVDKRHEADVQAKRLAEESDRMKSAFIANMSHEIR
HHHHHHHCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHH
TPLNAIVGFSTLLAESDDEEEKKEYLRIIESSNEFLLQLIGDILDISKIESGKMEFIYTT
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEEEH
LRLSEIFAPQQQAFALRVAPGVKVIFESDGNDYCIVSEKTRLTQVLTNFLSNAVKFTSSG
HHHHHHHCCCHHCEEEEECCCEEEEEECCCCCEEEEECHHHHHHHHHHHHHHHEEECCCC
SIRFGYRLTDDGIYVYVTDTGMGISKESQASIFNRFVKLNSFKQGTGLGLSICKTIIEKL
CEEEEEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
KGKIGVESEEGKGSTFWFTIPCQPMKVK
CCCCCCCCCCCCCCEEEEEECCCCCCCC
>Mature Secondary Structure
MIKKINMEVNTTENKTLEQDYRDLAGLLSNIVDSLPIYFFVKDTGDNFRYVYSSPLMNQL
CCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEEECCHHHHHH
YGRYHNDVVGKTDFDLFLDPVVAQSFRDMDEEVVKTGKMQRFVEQMIDPKGILRTMDTMK
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LLAPREDKPPYLVGISWDITKQRQVEEELHSYNKRLALACQAGKIYPWLWDLVNGTAELS
HHCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHCCCEEEE
LEENGQIKHVQITHASFTEKIHPDYRKVYENITTAFMRGEIDSMRFSFPCRYFSDEYVWL
ECCCCCEEEEEEECHHHHHHCCHHHHHHHHHHHHHHHHCCHHCEEEECCCCCCCCCCHHH
EKIGEVYEADPDGKPIRSIGILRDMTVDKRHEADVQAKRLAEESDRMKSAFIANMSHEIR
HHHHHHHCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHH
TPLNAIVGFSTLLAESDDEEEKKEYLRIIESSNEFLLQLIGDILDISKIESGKMEFIYTT
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEEEH
LRLSEIFAPQQQAFALRVAPGVKVIFESDGNDYCIVSEKTRLTQVLTNFLSNAVKFTSSG
HHHHHHHCCCHHCEEEEECCCEEEEEECCCCCEEEEECHHHHHHHHHHHHHHHEEECCCC
SIRFGYRLTDDGIYVYVTDTGMGISKESQASIFNRFVKLNSFKQGTGLGLSICKTIIEKL
CEEEEEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
KGKIGVESEEGKGSTFWFTIPCQPMKVK
CCCCCCCCCCCCCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]