Click here to switch to the map view.

The map label for this gene is mscL

Identifier: 150006791

GI number: 150006791

Start: 127328

End: 127750

Strand: Reverse

Name: mscL

Synonym: BDI_0117

Alternate gene names: 150006791

Gene position: 127750-127328 (Counterclockwise)

Preceding gene: 150006792

Following gene: 150006790

Centisome position: 2.66

GC content: 43.26

Gene sequence:

>423_bases
ATGAAAAAAATATTACAAGAGTTTAAGCAGTTCGCCATGCGAGGTAACGTAGTGGACATGGCGGTAGGTATCATTATCGG
TGGTGCCTTTGGAAAGATCGTATCTTCTATCGTAGCGGATCTGATCATGCCGGCGGTAGGTTTACTTGTAGGTGGCGTCA
ATTTCACGGATTTAAAAATCACCTTGAAGCACGCTGTAATGGAGGGAGACAAGGTGATCTCTCCGGCGGTCTCCATTAAC
TACGGAAATTTCATACAAGTGACACTGGATTTTATAATCATTGCTTTCGCCGTCTTTTTACTTGTCAAAGGCGTTAATGC
TTTAAGCAAGAAGAAGGAAGAAGCCCCGAAAGCTCCGGTCGCTCCCCCTGCGGATATCCAATTACTTACCGAGATTAGGG
ATTTACTGAAAAATAACAAATAA

Upstream 100 bases:

>100_bases
ACATGAAAATCAAAAATAATTTCTTCAAATTTCGCTATAAGCATTGAAAATATTCAACTTATGGCTAATTTTGTTGTTAT
AATATAAAAAGCGTTGTTTT

Downstream 100 bases:

>100_bases
ACACCAACCTTATCGGTTGACTTGCTGTTTCATAATTACAAAAAATAATGTTTTAACTCATAAAAAGTAGTAGTTATGAA
AAATGTATTATTCGGTTTAG

Product: large conductance mechanosensitive channel protein

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 140; Mature: 140

Protein sequence:

>140_residues
MKKILQEFKQFAMRGNVVDMAVGIIIGGAFGKIVSSIVADLIMPAVGLLVGGVNFTDLKITLKHAVMEGDKVISPAVSIN
YGNFIQVTLDFIIIAFAVFLLVKGVNALSKKKEEAPKAPVAPPADIQLLTEIRDLLKNNK

Sequences:

>Translated_140_residues
MKKILQEFKQFAMRGNVVDMAVGIIIGGAFGKIVSSIVADLIMPAVGLLVGGVNFTDLKITLKHAVMEGDKVISPAVSIN
YGNFIQVTLDFIIIAFAVFLLVKGVNALSKKKEEAPKAPVAPPADIQLLTEIRDLLKNNK
>Mature_140_residues
MKKILQEFKQFAMRGNVVDMAVGIIIGGAFGKIVSSIVADLIMPAVGLLVGGVNFTDLKITLKHAVMEGDKVISPAVSIN
YGNFIQVTLDFIIIAFAVFLLVKGVNALSKKKEEAPKAPVAPPADIQLLTEIRDLLKNNK

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family

Homologues:

Organism=Escherichia coli, GI1789686, Length=139, Percent_Identity=58.273381294964, Blast_Score=152, Evalue=6e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSCL_PARD8 (A6L898)

Other databases:

- EMBL:   CP000140
- RefSeq:   YP_001301534.1
- ProteinModelPortal:   A6L898
- SMR:   A6L898
- STRING:   A6L898
- GeneID:   5305208
- GenomeReviews:   CP000140_GR
- KEGG:   pdi:BDI_0117
- eggNOG:   COG1970
- HOGENOM:   HBG727604
- OMA:   IQLLTEI
- ProtClustDB:   CLSK826850
- BioCyc:   PDIS435591:BDI_0117-MONOMER
- HAMAP:   MF_00115
- InterPro:   IPR019823
- InterPro:   IPR001185
- PRINTS:   PR01264
- TIGRFAMs:   TIGR00220

Pfam domain/function: PF01741 MscL; SSF81330 MS_channel

EC number: NA

Molecular weight: Translated: 15047; Mature: 15047

Theoretical pI: Translated: 10.14; Mature: 10.14

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xd6fd560)-; HASH(0x1f99907c)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKILQEFKQFAMRGNVVDMAVGIIIGGAFGKIVSSIVADLIMPAVGLLVGGVNFTDLKI
CHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
TLKHAVMEGDKVISPAVSINYGNFIQVTLDFIIIAFAVFLLVKGVNALSKKKEEAPKAPV
HHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
APPADIQLLTEIRDLLKNNK
CCCHHHHHHHHHHHHHHCCH
>Mature Secondary Structure
MKKILQEFKQFAMRGNVVDMAVGIIIGGAFGKIVSSIVADLIMPAVGLLVGGVNFTDLKI
CHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
TLKHAVMEGDKVISPAVSINYGNFIQVTLDFIIIAFAVFLLVKGVNALSKKKEEAPKAPV
HHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
APPADIQLLTEIRDLLKNNK
CCCHHHHHHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA