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The map label for this gene is pepT [H]

Identifier: 150006318

GI number: 150006318

Start: 4727340

End: 4728584

Strand: Reverse

Name: pepT [H]

Synonym: BVU_3829

Alternate gene names: 150006318

Gene position: 4728584-4727340 (Counterclockwise)

Preceding gene: 150006319

Following gene: 150006317

Centisome position: 91.58

GC content: 42.73

Gene sequence:

>1245_bases
ATGAATATTAAAACAAAAAGAAAAATGACGGTAATAGAACGATTTTTAAAATATGTAAGCTTCGACACTCAGTCTGATGA
GAATAGCGGGGCTACCCCCAGTACACCGAAGCAGATGGTGTTTGCACAATATTTAAGATCTGAATTGGAACAATTGGGCT
TTCAGGAAATTTCTTTGGATGAGAATGGTTATCTGTTTGCCACATGGCCGGCCAATACGGATAAACCGGTTCCTACCATC
GGATTTATTGCACACATGGATACCAGTCCCGATATGACGGGTGCGGGAGTCACCCCACGCATTGTCTATGGTTATGACGG
AACGGATATTGTATTATGCGAAGAAGACAACATTATACTTTCTCCCAAGCAATTTCCCGAACTGTTGGATCATAAAGGGG
AGGATCTTATTGTAACCAACGGCAAAACATTGTTGGGAGCTGATGACAAGGCGGGTATTGCTGAAATCATCTCAGCTATG
GTATATTTGAAAGAACATCCTGAAATCAAGCATGGAAAAATTCGTGTGGGATTTAATCCTGATGAAGAAATAGGCTTGGG
TGCACATAAATTTGATGTGGAGAGATTCGGATGCGAGTGGGCATATACGATGGATGGTGGTGAAGTAGGGGAGTTGGAGT
TTGAAAACTTTAATGCCGCTTCTGCGAAAGTGATTATTAAAGGACGTAACGTACATCCGGGATATGCAAAGGATAAAATG
ATCAACTCCTTGCGTGTGGCTAATGAGTTTGTGTCTTTACAGCCTACGGATGAAGTGCCCGAATGTACTGATGGTTATCA
GGGATTCTATCATTTGATTGGCATGACGGGTGAAGTAGAGCAGACTACCATCTCTTATATAATCCGTGATCATGACCGTG
CCAAGTTTGAGGCATGCAAGGAAAATATGAAAAAGTGGGCGGAAATCATCAATAAGAAATATGGAGAAGGCACCGTTACA
TTGGAATTGAAAGACCAGTATTACAATATGCGTGAGAAAATTGAGCCGGTGATGCATATCATTGATATCGCTTTCAAGGC
TATGGAAGAGGCGGGCGTGACACCTAAGGTGAAGGCTATCCGCGGTGGAACGGATGGTGCACAACTTTCATTCAAGGGAT
TGCCTTGTCCCAATATTTTTGCCGGCGGCTTGAATTTTCATGGCAGATATGAGTTCGTGCCAATCCAGTCTATTGAGAAA
GCCATGAAAGTGGTGGTGAAAATCGCTGAATTGGTGGCAAGATAA

Upstream 100 bases:

>100_bases
GTTCCAGTTGCTTCTAATAACAAAATAATAAAATATTAATGAAAAAGAGCCGTGATTTGTTTGGCAATTCACGGCTCTTT
TTCTATTTTTACAGGCAAGT

Downstream 100 bases:

>100_bases
GTAATTTATTAACCTTAAAATAGTAAAGTAATAACATGAAAACAACTCCGTTTACCGAAACACACATTGCCTTGGGGGCG
AAAATGCACGAATTTGCAGG

Product: peptidase T

Products: NA

Alternate protein names: Aminotripeptidase; Tripeptidase; Tripeptide aminopeptidase [H]

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MNIKTKRKMTVIERFLKYVSFDTQSDENSGATPSTPKQMVFAQYLRSELEQLGFQEISLDENGYLFATWPANTDKPVPTI
GFIAHMDTSPDMTGAGVTPRIVYGYDGTDIVLCEEDNIILSPKQFPELLDHKGEDLIVTNGKTLLGADDKAGIAEIISAM
VYLKEHPEIKHGKIRVGFNPDEEIGLGAHKFDVERFGCEWAYTMDGGEVGELEFENFNAASAKVIIKGRNVHPGYAKDKM
INSLRVANEFVSLQPTDEVPECTDGYQGFYHLIGMTGEVEQTTISYIIRDHDRAKFEACKENMKKWAEIINKKYGEGTVT
LELKDQYYNMREKIEPVMHIIDIAFKAMEEAGVTPKVKAIRGGTDGAQLSFKGLPCPNIFAGGLNFHGRYEFVPIQSIEK
AMKVVVKIAELVAR

Sequences:

>Translated_414_residues
MNIKTKRKMTVIERFLKYVSFDTQSDENSGATPSTPKQMVFAQYLRSELEQLGFQEISLDENGYLFATWPANTDKPVPTI
GFIAHMDTSPDMTGAGVTPRIVYGYDGTDIVLCEEDNIILSPKQFPELLDHKGEDLIVTNGKTLLGADDKAGIAEIISAM
VYLKEHPEIKHGKIRVGFNPDEEIGLGAHKFDVERFGCEWAYTMDGGEVGELEFENFNAASAKVIIKGRNVHPGYAKDKM
INSLRVANEFVSLQPTDEVPECTDGYQGFYHLIGMTGEVEQTTISYIIRDHDRAKFEACKENMKKWAEIINKKYGEGTVT
LELKDQYYNMREKIEPVMHIIDIAFKAMEEAGVTPKVKAIRGGTDGAQLSFKGLPCPNIFAGGLNFHGRYEFVPIQSIEK
AMKVVVKIAELVAR
>Mature_414_residues
MNIKTKRKMTVIERFLKYVSFDTQSDENSGATPSTPKQMVFAQYLRSELEQLGFQEISLDENGYLFATWPANTDKPVPTI
GFIAHMDTSPDMTGAGVTPRIVYGYDGTDIVLCEEDNIILSPKQFPELLDHKGEDLIVTNGKTLLGADDKAGIAEIISAM
VYLKEHPEIKHGKIRVGFNPDEEIGLGAHKFDVERFGCEWAYTMDGGEVGELEFENFNAASAKVIIKGRNVHPGYAKDKM
INSLRVANEFVSLQPTDEVPECTDGYQGFYHLIGMTGEVEQTTISYIIRDHDRAKFEACKENMKKWAEIINKKYGEGTVT
LELKDQYYNMREKIEPVMHIIDIAFKAMEEAGVTPKVKAIRGGTDGAQLSFKGLPCPNIFAGGLNFHGRYEFVPIQSIEK
AMKVVVKIAELVAR

Specific function: Cleaves the N-terminal amino acid of tripeptides [H]

COG id: COG2195

COG function: function code E; Di- and tripeptidases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20B family [H]

Homologues:

Organism=Escherichia coli, GI1787372, Length=409, Percent_Identity=53.3007334963325, Blast_Score=425, Evalue=1e-120,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR002933
- InterPro:   IPR011650
- InterPro:   IPR010161 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.4.11.4 [H]

Molecular weight: Translated: 46300; Mature: 46300

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00758 ARGE_DAPE_CPG2_1 ; PS00759 ARGE_DAPE_CPG2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIKTKRKMTVIERFLKYVSFDTQSDENSGATPSTPKQMVFAQYLRSELEQLGFQEISLD
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEC
ENGYLFATWPANTDKPVPTIGFIAHMDTSPDMTGAGVTPRIVYGYDGTDIVLCEEDNIIL
CCCEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEECCCCEEEEECCCCEEE
SPKQFPELLDHKGEDLIVTNGKTLLGADDKAGIAEIISAMVYLKEHPEIKHGKIRVGFNP
CCHHHHHHHCCCCCEEEEECCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCC
DEEIGLGAHKFDVERFGCEWAYTMDGGEVGELEFENFNAASAKVIIKGRNVHPGYAKDKM
CCCCCCCCEEECHHHHCCEEEEEECCCCCCCEEECCCCCCCEEEEEECCCCCCCCHHHHH
INSLRVANEFVSLQPTDEVPECTDGYQGFYHLIGMTGEVEQTTISYIIRDHDRAKFEACK
HHHHHHHHHHEECCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHEEHHHCCCCHHHHHHHH
ENMKKWAEIINKKYGEGTVTLELKDQYYNMREKIEPVMHIIDIAFKAMEEAGVTPKVKAI
HHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
RGGTDGAQLSFKGLPCPNIFAGGLNFHGRYEFVPIQSIEKAMKVVVKIAELVAR
ECCCCCCEEEECCCCCCCCEECCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNIKTKRKMTVIERFLKYVSFDTQSDENSGATPSTPKQMVFAQYLRSELEQLGFQEISLD
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEC
ENGYLFATWPANTDKPVPTIGFIAHMDTSPDMTGAGVTPRIVYGYDGTDIVLCEEDNIIL
CCCEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEECCCCEEEEECCCCEEE
SPKQFPELLDHKGEDLIVTNGKTLLGADDKAGIAEIISAMVYLKEHPEIKHGKIRVGFNP
CCHHHHHHHCCCCCEEEEECCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCC
DEEIGLGAHKFDVERFGCEWAYTMDGGEVGELEFENFNAASAKVIIKGRNVHPGYAKDKM
CCCCCCCCEEECHHHHCCEEEEEECCCCCCCEEECCCCCCCEEEEEECCCCCCCCHHHHH
INSLRVANEFVSLQPTDEVPECTDGYQGFYHLIGMTGEVEQTTISYIIRDHDRAKFEACK
HHHHHHHHHHEECCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHEEHHHCCCCHHHHHHHH
ENMKKWAEIINKKYGEGTVTLELKDQYYNMREKIEPVMHIIDIAFKAMEEAGVTPKVKAI
HHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
RGGTDGAQLSFKGLPCPNIFAGGLNFHGRYEFVPIQSIEKAMKVVVKIAELVAR
ECCCCCCEEEECCCCCCCCEECCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12663928 [H]