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The map label for this gene is purF [H]

Identifier: 150006319

GI number: 150006319

Start: 4728668

End: 4730074

Strand: Reverse

Name: purF [H]

Synonym: BVU_3830

Alternate gene names: 150006319

Gene position: 4730074-4728668 (Counterclockwise)

Preceding gene: 150006321

Following gene: 150006318

Centisome position: 91.61

GC content: 42.43

Gene sequence:

>1407_bases
ATGGGTGGTTTTTTTGGAACAGTCTCAAAGGCTGAATGCGTCACCGACTTATTTTACGGGACGGACTATAATTCACATTT
AGGAACAAAAAGAGGTGGTTTGGCTACGTATGCCCAAGGAGAGGGATTTATGCGTTCTATCCACAATTTGGAGAGTTCTT
ATTTCCGCACGAAGTTTGAATCTGAACTTCCAAAGTTCAAAGGTAGTTCGGGTATCGGCATTATCAGTGATACGGACCCG
CAGCCTATCGTGATAAACTCGCATCTGGGCAAGTTCGCCATCGTCACAGTAGCTAAAATCAATAACATTACGGAATTGGA
ACGCGACTTGCTGGCAGCTAATATGCACTTTTCTGAAATGAGTCTTGGCAAGACGAATCAGACGGAGCTGATCGCATTGC
TTATTGTGCAAGGGAAAAATTTTGTAGATGGTATAGAGAATGTCTTTAATAAAATCAAAGGATCATGCTCCATGCTGATA
TTGACGGAAGATGGTATTATAGCAGCACGTGACAAATGGGGACGTACTCCGATTGTGATAGGTAAAAAAGAGGGTGCGTA
TGCTGCGACAAGTGAATCAAGCAGTTTGCCGAATCTGGATTATGAAATAGAGAAATATGTAGGACCGGGCGAAATTATTC
GCTTGCGTGCCGACGGAATGGAACAAATGCGCAAGCCGAACGAAGGAATGCAGATTTGTTCTTTTTTGTGGGTATATTAT
GGATTTCCTGTTTCATGCTATGAAGGGAAAAATGTAGAGGAAGTACGTTTTATGAGTGGTTATAAAATGGGGCAGAAGGA
CGATTCGGAAGTTGATTGTGCCTGCGGTATTCCTGATTCGGGAGTAGGCATGGCATTGGGGTATGCTGAAGGCAAAGGAA
TTCCTTATCATCGTGCTATTGCCAAGTACACACCCACCTGGCCTCGTAGTTTTACTCCTGCCAATCAGGAAATGCGTTCA
CTGGTAGCAAAAATGAAGCTGATACCTAATCGTGCAATGCTGGAAGGTAAGCGCGTGTTGTTCTGTGACGATTCTATAGT
ACGTGGAACCCAACTTCGTGATAATGTAAAAATTTTATTTGATTATGGTGCCAAAGAGGTTCATATGCGCATTGCTTGTC
CGCCATTGATTTATGGATGCCCTTTCATTGGTTTCACTTCTTCAAAAAGTGATTTGGAATTGATAACCCGCCGGATGATT
AAGGAGATTGAAGGCGATGAAAATAAGAATCTGGAAAAATATGCAACGACAGATTCGCCGGAGTATAAGAAGATGGTGCA
AATGATTGCCGACCGCTTCGGTTTGAGCTCCTTGAAATTCAATACTTTGGAAACTTTGGTAGAAGCGATAGGATTACCAA
AATGTAAGGTATGTACCCATTGTTTTGATGGTTCCAGTTGCTTCTAA

Upstream 100 bases:

>100_bases
ATAAATGCTACATTTTAATGCAAAAATAGCAATGATTATTCATTCAGAGCCAAACTTTTTTCGTAATTTTGCACCCTAAA
CGAAAAATACGCAAAAAGGA

Downstream 100 bases:

>100_bases
TAACAAAATAATAAAATATTAATGAAAAAGAGCCGTGATTTGTTTGGCAATTCACGGCTCTTTTTCTATTTTTACAGGCA
AGTATGAATATTAAAACAAA

Product: amidophosphoribosyltransferase precursor

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MGGFFGTVSKAECVTDLFYGTDYNSHLGTKRGGLATYAQGEGFMRSIHNLESSYFRTKFESELPKFKGSSGIGIISDTDP
QPIVINSHLGKFAIVTVAKINNITELERDLLAANMHFSEMSLGKTNQTELIALLIVQGKNFVDGIENVFNKIKGSCSMLI
LTEDGIIAARDKWGRTPIVIGKKEGAYAATSESSSLPNLDYEIEKYVGPGEIIRLRADGMEQMRKPNEGMQICSFLWVYY
GFPVSCYEGKNVEEVRFMSGYKMGQKDDSEVDCACGIPDSGVGMALGYAEGKGIPYHRAIAKYTPTWPRSFTPANQEMRS
LVAKMKLIPNRAMLEGKRVLFCDDSIVRGTQLRDNVKILFDYGAKEVHMRIACPPLIYGCPFIGFTSSKSDLELITRRMI
KEIEGDENKNLEKYATTDSPEYKKMVQMIADRFGLSSLKFNTLETLVEAIGLPKCKVCTHCFDGSSCF

Sequences:

>Translated_468_residues
MGGFFGTVSKAECVTDLFYGTDYNSHLGTKRGGLATYAQGEGFMRSIHNLESSYFRTKFESELPKFKGSSGIGIISDTDP
QPIVINSHLGKFAIVTVAKINNITELERDLLAANMHFSEMSLGKTNQTELIALLIVQGKNFVDGIENVFNKIKGSCSMLI
LTEDGIIAARDKWGRTPIVIGKKEGAYAATSESSSLPNLDYEIEKYVGPGEIIRLRADGMEQMRKPNEGMQICSFLWVYY
GFPVSCYEGKNVEEVRFMSGYKMGQKDDSEVDCACGIPDSGVGMALGYAEGKGIPYHRAIAKYTPTWPRSFTPANQEMRS
LVAKMKLIPNRAMLEGKRVLFCDDSIVRGTQLRDNVKILFDYGAKEVHMRIACPPLIYGCPFIGFTSSKSDLELITRRMI
KEIEGDENKNLEKYATTDSPEYKKMVQMIADRFGLSSLKFNTLETLVEAIGLPKCKVCTHCFDGSSCF
>Mature_467_residues
GGFFGTVSKAECVTDLFYGTDYNSHLGTKRGGLATYAQGEGFMRSIHNLESSYFRTKFESELPKFKGSSGIGIISDTDPQ
PIVINSHLGKFAIVTVAKINNITELERDLLAANMHFSEMSLGKTNQTELIALLIVQGKNFVDGIENVFNKIKGSCSMLIL
TEDGIIAARDKWGRTPIVIGKKEGAYAATSESSSLPNLDYEIEKYVGPGEIIRLRADGMEQMRKPNEGMQICSFLWVYYG
FPVSCYEGKNVEEVRFMSGYKMGQKDDSEVDCACGIPDSGVGMALGYAEGKGIPYHRAIAKYTPTWPRSFTPANQEMRSL
VAKMKLIPNRAMLEGKRVLFCDDSIVRGTQLRDNVKILFDYGAKEVHMRIACPPLIYGCPFIGFTSSKSDLELITRRMIK
EIEGDENKNLEKYATTDSPEYKKMVQMIADRFGLSSLKFNTLETLVEAIGLPKCKVCTHCFDGSSCF

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=393, Percent_Identity=26.972010178117, Blast_Score=135, Evalue=6e-32,
Organism=Escherichia coli, GI1788651, Length=396, Percent_Identity=28.030303030303, Blast_Score=117, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17554892, Length=361, Percent_Identity=32.409972299169, Blast_Score=161, Evalue=6e-40,
Organism=Saccharomyces cerevisiae, GI6323958, Length=494, Percent_Identity=22.4696356275304, Blast_Score=83, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24659604, Length=482, Percent_Identity=26.7634854771784, Blast_Score=148, Evalue=8e-36,
Organism=Drosophila melanogaster, GI24659598, Length=482, Percent_Identity=26.7634854771784, Blast_Score=148, Evalue=8e-36,
Organism=Drosophila melanogaster, GI28573187, Length=481, Percent_Identity=26.4033264033264, Blast_Score=137, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 51990; Mature: 51859

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGFFGTVSKAECVTDLFYGTDYNSHLGTKRGGLATYAQGEGFMRSIHNLESSYFRTKFE
CCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHH
SELPKFKGSSGIGIISDTDPQPIVINSHLGKFAIVTVAKINNITELERDLLAANMHFSEM
HHCCCCCCCCCEEEEECCCCCCEEEECCCCCEEEEEEEHHCCHHHHHHHHHHHCCCHHHH
SLGKTNQTELIALLIVQGKNFVDGIENVFNKIKGSCSMLILTEDGIIAARDKWGRTPIVI
CCCCCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCEEEEEEECCCEEEECCCCCCCCEEE
GKKEGAYAATSESSSLPNLDYEIEKYVGPGEIIRLRADGMEQMRKPNEGMQICSFLWVYY
ECCCCCEEECCCCCCCCCCCCHHHHCCCCCCEEEEECCCHHHHHCCCCHHHHHHHHHHHH
GFPVSCYEGKNVEEVRFMSGYKMGQKDDSEVDCACGIPDSGVGMALGYAEGKGIPYHRAI
CCCEEEECCCCHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCEEEEECCCCCCCHHHHH
AKYTPTWPRSFTPANQEMRSLVAKMKLIPNRAMLEGKRVLFCDDSIVRGTQLRDNVKILF
HHCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHCCCEEEEECCCHHCCCCCCCCEEEEE
DYGAKEVHMRIACPPLIYGCPFIGFTSSKSDLELITRRMIKEIEGDENKNLEKYATTDSP
ECCCCEEEEEEECCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCH
EYKKMVQMIADRFGLSSLKFNTLETLVEAIGLPKCKVCTHCFDGSSCF
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCCC
>Mature Secondary Structure 
GGFFGTVSKAECVTDLFYGTDYNSHLGTKRGGLATYAQGEGFMRSIHNLESSYFRTKFE
CCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHH
SELPKFKGSSGIGIISDTDPQPIVINSHLGKFAIVTVAKINNITELERDLLAANMHFSEM
HHCCCCCCCCCEEEEECCCCCCEEEECCCCCEEEEEEEHHCCHHHHHHHHHHHCCCHHHH
SLGKTNQTELIALLIVQGKNFVDGIENVFNKIKGSCSMLILTEDGIIAARDKWGRTPIVI
CCCCCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCEEEEEEECCCEEEECCCCCCCCEEE
GKKEGAYAATSESSSLPNLDYEIEKYVGPGEIIRLRADGMEQMRKPNEGMQICSFLWVYY
ECCCCCEEECCCCCCCCCCCCHHHHCCCCCCEEEEECCCHHHHHCCCCHHHHHHHHHHHH
GFPVSCYEGKNVEEVRFMSGYKMGQKDDSEVDCACGIPDSGVGMALGYAEGKGIPYHRAI
CCCEEEECCCCHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCEEEEECCCCCCCHHHHH
AKYTPTWPRSFTPANQEMRSLVAKMKLIPNRAMLEGKRVLFCDDSIVRGTQLRDNVKILF
HHCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHCCCEEEEECCCHHCCCCCCCCEEEEE
DYGAKEVHMRIACPPLIYGCPFIGFTSSKSDLELITRRMIKEIEGDENKNLEKYATTDSP
ECCCCEEEEEEECCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCH
EYKKMVQMIADRFGLSSLKFNTLETLVEAIGLPKCKVCTHCFDGSSCF
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]