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The map label for this gene is sgcX [C]

Identifier: 150006240

GI number: 150006240

Start: 4634689

End: 4635735

Strand: Reverse

Name: sgcX [C]

Synonym: BVU_3751

Alternate gene names: 150006240

Gene position: 4635735-4634689 (Counterclockwise)

Preceding gene: 150006247

Following gene: 150006239

Centisome position: 89.78

GC content: 33.24

Gene sequence:

>1047_bases
ATGGAGTTTAATACAAGTTTTCTAAAGCATGTATCAAGTATAATTGCACCATCCGGTTATGAAAAAGAAGTTATTGATGT
ATGGTGCAAAGAAATGTCACAATATGTTGATGAGATTAATCTTACAAATATAGGTAATGCTATAGCAGTAAAACATGGCT
GTGGAGATACACGAAAAAAAATAATGATAACTGCTCATGCCGATGAAATTGGAATAATAGTAACTTATATTGATAATAAT
GGTTATTTGTATTTTGATGAAATAGGTGGAATAGATACTAATATTCTTCCGGGTAGAACAGTGTTGATAAAAGGTGTTGA
TAATCGTATTATTAAAGGAGTTATAGGAGTTAAACCAATTCATCTCCAAAAGGATTCTGGTTCTAAGCAATCATTAAATC
CTGAAGATTTGTGGATTGATATTTGCGTAAGTAATAAAGAATCAGCTTTAGAGTTAGTAGAAGTTGGTAGTATAGGAACA
TTGGAATCAGTAGCATATCAACAAGGTACAAAGGTGATAGGAAAGGCTATGGACAATAGATGCTCCCTTTCAGCAATGGT
TTCTATAGCTAAAATACTTGCAGAAAAAAAATGTAATGATGACATATTTTATGTTGCCACAACTCAGGAAGAACTTAGAG
GAAGAGGAGCGCAGACTGCTGCATTTGCTATAAATCCGGATATATGTATAGTTTTGGATGTAACTCATGCAACAGATTAC
CCTGGAATGTCTCCAATAAGAGATGGTGATATAAATTTGGGAAAAGGTGTTGTTGTTGCAATTGGTCCCAATATGGATAA
TGAAATTACGAAAAAAGTTGTATCTTTGGCAAAGAATTTGAATATCAGCTATCAGGTAGAGGTTTGTGCACGTCCTACCG
GTACTGATGCTAATGTGGTTCAGATTAGCCGTGATGGAATACGTACATTGTTATTGAGTATTCCTTGTAGATATATGCAT
AGTCCTGTAGAGACCATAGATATAGAAGATTTAAAAAGTTGCTGTACTTTAATAACTAAGTTAATAGAGAACAGACATTA
TTTATAA

Upstream 100 bases:

>100_bases
TTAATTATTTTAATATAATAGTGATCAATTGTCATTTTAAGCTGCTTTTGATGATATTCAAATAAGTTTTTGTAAATTTG
TGTTCTAAAAAAAATTGAAT

Downstream 100 bases:

>100_bases
TTATAAATTTTAAAATAAATTAAAATGAAAAAGAAAATTACATTCAATTGGTCAGAAAAAAAGATTTCTGATGCTGAACG
CGTAGGGGCTATGTATTGCT

Product: putative cellulase M and related protein

Products: D-glucose; GDP

Alternate protein names: NA

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MEFNTSFLKHVSSIIAPSGYEKEVIDVWCKEMSQYVDEINLTNIGNAIAVKHGCGDTRKKIMITAHADEIGIIVTYIDNN
GYLYFDEIGGIDTNILPGRTVLIKGVDNRIIKGVIGVKPIHLQKDSGSKQSLNPEDLWIDICVSNKESALELVEVGSIGT
LESVAYQQGTKVIGKAMDNRCSLSAMVSIAKILAEKKCNDDIFYVATTQEELRGRGAQTAAFAINPDICIVLDVTHATDY
PGMSPIRDGDINLGKGVVVAIGPNMDNEITKKVVSLAKNLNISYQVEVCARPTGTDANVVQISRDGIRTLLLSIPCRYMH
SPVETIDIEDLKSCCTLITKLIENRHYL

Sequences:

>Translated_348_residues
MEFNTSFLKHVSSIIAPSGYEKEVIDVWCKEMSQYVDEINLTNIGNAIAVKHGCGDTRKKIMITAHADEIGIIVTYIDNN
GYLYFDEIGGIDTNILPGRTVLIKGVDNRIIKGVIGVKPIHLQKDSGSKQSLNPEDLWIDICVSNKESALELVEVGSIGT
LESVAYQQGTKVIGKAMDNRCSLSAMVSIAKILAEKKCNDDIFYVATTQEELRGRGAQTAAFAINPDICIVLDVTHATDY
PGMSPIRDGDINLGKGVVVAIGPNMDNEITKKVVSLAKNLNISYQVEVCARPTGTDANVVQISRDGIRTLLLSIPCRYMH
SPVETIDIEDLKSCCTLITKLIENRHYL
>Mature_348_residues
MEFNTSFLKHVSSIIAPSGYEKEVIDVWCKEMSQYVDEINLTNIGNAIAVKHGCGDTRKKIMITAHADEIGIIVTYIDNN
GYLYFDEIGGIDTNILPGRTVLIKGVDNRIIKGVIGVKPIHLQKDSGSKQSLNPEDLWIDICVSNKESALELVEVGSIGT
LESVAYQQGTKVIGKAMDNRCSLSAMVSIAKILAEKKCNDDIFYVATTQEELRGRGAQTAAFAINPDICIVLDVTHATDY
PGMSPIRDGDINLGKGVVVAIGPNMDNEITKKVVSLAKNLNISYQVEVCARPTGTDANVVQISRDGIRTLLLSIPCRYMH
SPVETIDIEDLKSCCTLITKLIENRHYL

Specific function: Unknown

COG id: COG1363

COG function: function code G; Cellulase M and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M42 family [H]

Homologues:

Organism=Escherichia coli, GI87082417, Length=346, Percent_Identity=30.635838150289, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1790332, Length=346, Percent_Identity=29.1907514450867, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI1788727, Length=352, Percent_Identity=23.5795454545455, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008007 [H]

Pfam domain/function: PF05343 Peptidase_M42 [H]

EC number: 3.2.1.4

Molecular weight: Translated: 38104; Mature: 38104

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFNTSFLKHVSSIIAPSGYEKEVIDVWCKEMSQYVDEINLTNIGNAIAVKHGCGDTRKK
CCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCE
IMITAHADEIGIIVTYIDNNGYLYFDEIGGIDTNILPGRTVLIKGVDNRIIKGVIGVKPI
EEEEEECCCEEEEEEEEECCCEEEEECCCCCCCCCCCCCEEEEECCCCHHHHHHHCCCEE
HLQKDSGSKQSLNPEDLWIDICVSNKESALELVEVGSIGTLESVAYQQGTKVIGKAMDNR
EEECCCCCCCCCCCCCEEEEEEECCCCHHHHHEECCCCCCHHHHHHHHHHHHHHHHHCCC
CSLSAMVSIAKILAEKKCNDDIFYVATTQEELRGRGAQTAAFAINPDICIVLDVTHATDY
CHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHCCCCCCEEEEEECCCEEEEEEECCCCCC
PGMSPIRDGDINLGKGVVVAIGPNMDNEITKKVVSLAKNLNISYQVEVCARPTGTDANVV
CCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCEE
QISRDGIRTLLLSIPCRYMHSPVETIDIEDLKSCCTLITKLIENRHYL
EEEHHHHHHHHHHCCHHHHCCCCCEECHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MEFNTSFLKHVSSIIAPSGYEKEVIDVWCKEMSQYVDEINLTNIGNAIAVKHGCGDTRKK
CCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCE
IMITAHADEIGIIVTYIDNNGYLYFDEIGGIDTNILPGRTVLIKGVDNRIIKGVIGVKPI
EEEEEECCCEEEEEEEEECCCEEEEECCCCCCCCCCCCCEEEEECCCCHHHHHHHCCCEE
HLQKDSGSKQSLNPEDLWIDICVSNKESALELVEVGSIGTLESVAYQQGTKVIGKAMDNR
EEECCCCCCCCCCCCCEEEEEEECCCCHHHHHEECCCCCCHHHHHHHHHHHHHHHHHCCC
CSLSAMVSIAKILAEKKCNDDIFYVATTQEELRGRGAQTAAFAINPDICIVLDVTHATDY
CHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHCCCCCCEEEEEECCCEEEEEEECCCCCC
PGMSPIRDGDINLGKGVVVAIGPNMDNEITKKVVSLAKNLNISYQVEVCARPTGTDANVV
CCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCEE
QISRDGIRTLLLSIPCRYMHSPVETIDIEDLKSCCTLITKLIENRHYL
EEEHHHHHHHHHHCCHHHHCCCCCEECHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDPglucose; H2O

Specific reaction: GDP-glucose + H2O = D-glucose + GDP

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]