Click here to switch to the map view.

The map label for this gene is hmrM [H]

Identifier: 150004752

GI number: 150004752

Start: 2858525

End: 2859847

Strand: Direct

Name: hmrM [H]

Synonym: BVU_2213

Alternate gene names: 150004752

Gene position: 2858525-2859847 (Clockwise)

Preceding gene: 150004750

Following gene: 150004753

Centisome position: 55.36

GC content: 43.46

Gene sequence:

>1323_bases
ATGTATACCAACAAAGAAATATGGAACGTAAGTTATCCGATTTTTCTCGGATTATTGGCGCAAAACATTATCAATGTGAC
AGATACTGCTTTCCTGGGCAGGGTAGGAGAAGTCGAGTTGGGAGCTTCCGCCATGGGTGGATTGTTTTATATCTGTGTCT
TCACTATTGCTTTCGGTTTTAGTGTCGGTTCACAAATCTTGATTGCTCGTCGTAATGGAGAAGGACGTTACAAAGATGTG
GGTCCCGTAATGATACAGGGAGGGACATTCTTGCTGGGATTGGCGGTGCTCATGTTTGGGCTGACACATCTTTTGGCCCC
ATCCATTGTCCGCTTGCTCATTTCATCTGATTCCATTTTTGATGCTACCATGGAATTTCTCAACTGGCGTATTTGGGGCT
TTTTCTTCGCCTTTGTCAACGTGATGTTCCGTGCGCTGTATATTGGTATTACACGTACTAAGGTGCTGACAATGAATGCA
GTGGTCATGGCATTGGTGAATGTGCTGTTGGACTATATTTTGATTTTCGGTAAGTTTGGAATGCCGGAGATGGGGATTAA
AGGAGCAGCTGTCGCTTCGGTGATGGCTGAAGCCGCTTCATTGGCTTTTTTCTTGATTTATACCTACGCAAAGGTGGATT
TTAAGAAATTCGGCTTGAATCACTGGCAGAAGATTGACTTTTCTTTATTGGGAAAAATTCTGAGTATTTCGTGTTTTACC
ATGATACAATATTTTTTGGCCATGGCTACTTGGTTTGTCTTTTTCATCGCGATAGAACGGTTGGGTCAACGCGAGCTGGC
GATTGCCAATATCGTGCGTAGTATCTATATTGTGATGCTCATTCCGGTTCAGGCATTGTCTACTACTACCAATTCATTGG
TCAGCAATCTGATTGGTGCGGGGGGGATAACCCATGTCATGCGTTTGATTTGGCGTATAGCCCGGATGTCGTTTCTCATT
ATGGTGGTCTGTGTGGCGGTGGTGGTGCTTTTTCCTCATGCCATGCTTTCGGTTTACACCAACGAACCGGCGTTGCTGGT
AGAGTCTGTGCCTTCGTTGTATGTCATTGCCGGAGCGATGATTATTGCATCAGTGGCGAATATTTATTTCAATGCCATTT
CGGGAACTGGAAATACGCAGGCTGCTCTGATACTTGAAATGGGGACTTTGGTATTCTACGCCCTTTACATTCTATGGATA
GGCATGGTGGTGAAAGCACCGGTTTCTGTTTGTTTTTCTATAGAGGTGGTGTACTATAGTTTGTTACTGCTTAGCAGCAT
TATTTATTTGAAAAAAGCAAAATGGCAGAATAAAAAGATATAA

Upstream 100 bases:

>100_bases
AATTCCTGGTCTGTTTACAGTTTCTTCACTCCATTCAGAACACCGGATTGAAAAGAATGTAGTATCTTTGCAACTCTTTT
ACTCAATGCAAAAAAAATAT

Downstream 100 bases:

>100_bases
GGTTCATAAGTTTTTTGTATTTTTGCACCCTGTTGTTATTCTGAAGGTTAGTAAAGAAGGACAGGCAATCATAATTTGTA
ATTTATAATTAAATATATAT

Product: putative antiporter membrane protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter; Na(+)/drug antiporter [H]

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MYTNKEIWNVSYPIFLGLLAQNIINVTDTAFLGRVGEVELGASAMGGLFYICVFTIAFGFSVGSQILIARRNGEGRYKDV
GPVMIQGGTFLLGLAVLMFGLTHLLAPSIVRLLISSDSIFDATMEFLNWRIWGFFFAFVNVMFRALYIGITRTKVLTMNA
VVMALVNVLLDYILIFGKFGMPEMGIKGAAVASVMAEAASLAFFLIYTYAKVDFKKFGLNHWQKIDFSLLGKILSISCFT
MIQYFLAMATWFVFFIAIERLGQRELAIANIVRSIYIVMLIPVQALSTTTNSLVSNLIGAGGITHVMRLIWRIARMSFLI
MVVCVAVVVLFPHAMLSVYTNEPALLVESVPSLYVIAGAMIIASVANIYFNAISGTGNTQAALILEMGTLVFYALYILWI
GMVVKAPVSVCFSIEVVYYSLLLLSSIIYLKKAKWQNKKI

Sequences:

>Translated_440_residues
MYTNKEIWNVSYPIFLGLLAQNIINVTDTAFLGRVGEVELGASAMGGLFYICVFTIAFGFSVGSQILIARRNGEGRYKDV
GPVMIQGGTFLLGLAVLMFGLTHLLAPSIVRLLISSDSIFDATMEFLNWRIWGFFFAFVNVMFRALYIGITRTKVLTMNA
VVMALVNVLLDYILIFGKFGMPEMGIKGAAVASVMAEAASLAFFLIYTYAKVDFKKFGLNHWQKIDFSLLGKILSISCFT
MIQYFLAMATWFVFFIAIERLGQRELAIANIVRSIYIVMLIPVQALSTTTNSLVSNLIGAGGITHVMRLIWRIARMSFLI
MVVCVAVVVLFPHAMLSVYTNEPALLVESVPSLYVIAGAMIIASVANIYFNAISGTGNTQAALILEMGTLVFYALYILWI
GMVVKAPVSVCFSIEVVYYSLLLLSSIIYLKKAKWQNKKI
>Mature_440_residues
MYTNKEIWNVSYPIFLGLLAQNIINVTDTAFLGRVGEVELGASAMGGLFYICVFTIAFGFSVGSQILIARRNGEGRYKDV
GPVMIQGGTFLLGLAVLMFGLTHLLAPSIVRLLISSDSIFDATMEFLNWRIWGFFFAFVNVMFRALYIGITRTKVLTMNA
VVMALVNVLLDYILIFGKFGMPEMGIKGAAVASVMAEAASLAFFLIYTYAKVDFKKFGLNHWQKIDFSLLGKILSISCFT
MIQYFLAMATWFVFFIAIERLGQRELAIANIVRSIYIVMLIPVQALSTTTNSLVSNLIGAGGITHVMRLIWRIARMSFLI
MVVCVAVVVLFPHAMLSVYTNEPALLVESVPSLYVIAGAMIIASVANIYFNAISGTGNTQAALILEMGTLVFYALYILWI
GMVVKAPVSVCFSIEVVYYSLLLLSSIIYLKKAKWQNKKI

Specific function: Multidrug efflux pump that functions as a Na(+)/drug antiporter. Confers resistance to norfloxacin, acriflavine, doxorubicin, ethidium bromide, tetraphenylphosphonium chloride, daunomycin, berberine and sodium deoxycholate [H]

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family [H]

Homologues:

Organism=Escherichia coli, GI48994890, Length=186, Percent_Identity=27.4193548387097, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002528 [H]

Pfam domain/function: PF01554 MatE [H]

EC number: NA

Molecular weight: Translated: 48724; Mature: 48724

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYTNKEIWNVSYPIFLGLLAQNIINVTDTAFLGRVGEVELGASAMGGLFYICVFTIAFGF
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
SVGSQILIARRNGEGRYKDVGPVMIQGGTFLLGLAVLMFGLTHLLAPSIVRLLISSDSIF
CCCCEEEEEEECCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
DATMEFLNWRIWGFFFAFVNVMFRALYIGITRTKVLTMNAVVMALVNVLLDYILIFGKFG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
MPEMGIKGAAVASVMAEAASLAFFLIYTYAKVDFKKFGLNHWQKIDFSLLGKILSISCFT
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHH
MIQYFLAMATWFVFFIAIERLGQRELAIANIVRSIYIVMLIPVQALSTTTNSLVSNLIGA
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GGITHVMRLIWRIARMSFLIMVVCVAVVVLFPHAMLSVYTNEPALLVESVPSLYVIAGAM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHH
IIASVANIYFNAISGTGNTQAALILEMGTLVFYALYILWIGMVVKAPVSVCFSIEVVYYS
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLLLSSIIYLKKAKWQNKKI
HHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MYTNKEIWNVSYPIFLGLLAQNIINVTDTAFLGRVGEVELGASAMGGLFYICVFTIAFGF
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
SVGSQILIARRNGEGRYKDVGPVMIQGGTFLLGLAVLMFGLTHLLAPSIVRLLISSDSIF
CCCCEEEEEEECCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
DATMEFLNWRIWGFFFAFVNVMFRALYIGITRTKVLTMNAVVMALVNVLLDYILIFGKFG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
MPEMGIKGAAVASVMAEAASLAFFLIYTYAKVDFKKFGLNHWQKIDFSLLGKILSISCFT
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHH
MIQYFLAMATWFVFFIAIERLGQRELAIANIVRSIYIVMLIPVQALSTTTNSLVSNLIGA
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GGITHVMRLIWRIARMSFLIMVVCVAVVVLFPHAMLSVYTNEPALLVESVPSLYVIAGAM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHH
IIASVANIYFNAISGTGNTQAALILEMGTLVFYALYILWIGMVVKAPVSVCFSIEVVYYS
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLLLSSIIYLKKAKWQNKKI
HHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]