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The map label for this gene is rnhB [H]

Identifier: 148828223

GI number: 148828223

Start: 1653536

End: 1654129

Strand: Reverse

Name: rnhB [H]

Synonym: CGSHiGG_08925

Alternate gene names: 148828223

Gene position: 1654129-1653536 (Counterclockwise)

Preceding gene: 148828224

Following gene: 148828222

Centisome position: 87.65

GC content: 43.1

Gene sequence:

>594_bases
ATGTTTGAATATCCACAAGGCTATGAATTGATCGCTGGCGTTGATGAAGTTGGGCGAGGCCCTTTAGTTGGGGCGGTTGT
CACGGCTGCCGTTATTTTGGATCCGAATAATCCAATTGAAGGACTCGCTGATTCTAAAAAACTTTCAGAAAAAAAACGTT
TAGCCCTTGCTGACGAAATCAAAGAAAAAGCCCTTGCTTGGGCATTAGGTCGAGCTGAAGCTAACGAAATTGACGAGATA
AATATTCTGCAAGCCTCCTTGCTGGCAATGACTCGAGCGGTAAAATCTTTAAAAATTCAACCGCACTTTGTGTTGGTTGA
TGGCAATAAGATCCCAAAAGATTTAGCTATTCCAGCGCAAGCCGTCGTAAAAGGCGATAGTATTGTGGCTGAAATCAGTG
CAGCCTCTATTTTGGCGAAAGTGGCACGAGACCAAGAAATGGAAGAATTAGACAAGCAATATCCAGAATACGCTTTTGCC
CAGCATAAAGGCTATCCGACCAAATTACATTTGGAAAAATTAGCTGAATTTGGCGCGTTACCGCAACATCGTCGTAGCTT
TGCCCCAGTGAAAAAAGCACTAGAACAGTTTTAG

Upstream 100 bases:

>100_bases
GTTTTAATTCAGCACTTCACGGATTTACACCAAAAAATTCAATGCAATGCCGATAAGCAAGCGGCTCAAGCGGTCATTGA
TTTATTAGAAGGAAAAGAAA

Downstream 100 bases:

>100_bases
AATGCTAATTTGGTTTAACAAAGGCTGTGTTTTAATACTGTGTTGATGCTTTCACTAAAATTTGATACATAAACAAAATT
TTTTGACTTCTGTTAGTCAA

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII [H]

Number of amino acids: Translated: 197; Mature: 197

Protein sequence:

>197_residues
MFEYPQGYELIAGVDEVGRGPLVGAVVTAAVILDPNNPIEGLADSKKLSEKKRLALADEIKEKALAWALGRAEANEIDEI
NILQASLLAMTRAVKSLKIQPHFVLVDGNKIPKDLAIPAQAVVKGDSIVAEISAASILAKVARDQEMEELDKQYPEYAFA
QHKGYPTKLHLEKLAEFGALPQHRRSFAPVKKALEQF

Sequences:

>Translated_197_residues
MFEYPQGYELIAGVDEVGRGPLVGAVVTAAVILDPNNPIEGLADSKKLSEKKRLALADEIKEKALAWALGRAEANEIDEI
NILQASLLAMTRAVKSLKIQPHFVLVDGNKIPKDLAIPAQAVVKGDSIVAEISAASILAKVARDQEMEELDKQYPEYAFA
QHKGYPTKLHLEKLAEFGALPQHRRSFAPVKKALEQF
>Mature_197_residues
MFEYPQGYELIAGVDEVGRGPLVGAVVTAAVILDPNNPIEGLADSKKLSEKKRLALADEIKEKALAWALGRAEANEIDEI
NILQASLLAMTRAVKSLKIQPHFVLVDGNKIPKDLAIPAQAVVKGDSIVAEISAASILAKVARDQEMEELDKQYPEYAFA
QHKGYPTKLHLEKLAEFGALPQHRRSFAPVKKALEQF

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family [H]

Homologues:

Organism=Escherichia coli, GI1786380, Length=193, Percent_Identity=72.020725388601, Blast_Score=272, Evalue=8e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01351 RNase_HII [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 21550; Mature: 21550

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFEYPQGYELIAGVDEVGRGPLVGAVVTAAVILDPNNPIEGLADSKKLSEKKRLALADEI
CCCCCCCCHHHHCHHHHCCCCHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHH
KEKALAWALGRAEANEIDEINILQASLLAMTRAVKSLKIQPHFVLVDGNKIPKDLAIPAQ
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHCCCHH
AVVKGDSIVAEISAASILAKVARDQEMEELDKQYPEYAFAQHKGYPTKLHLEKLAEFGAL
HHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHCCC
PQHRRSFAPVKKALEQF
CHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFEYPQGYELIAGVDEVGRGPLVGAVVTAAVILDPNNPIEGLADSKKLSEKKRLALADEI
CCCCCCCCHHHHCHHHHCCCCHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHH
KEKALAWALGRAEANEIDEINILQASLLAMTRAVKSLKIQPHFVLVDGNKIPKDLAIPAQ
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHCCCHH
AVVKGDSIVAEISAASILAKVARDQEMEELDKQYPEYAFAQHKGYPTKLHLEKLAEFGAL
HHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHCCC
PQHRRSFAPVKKALEQF
CHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA