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The map label for this gene is pflB [H]

Identifier: 148827319

GI number: 148827319

Start: 651193

End: 651303

Strand: Reverse

Name: pflB [H]

Synonym: CGSHiGG_03500

Alternate gene names: 148827319

Gene position: 651303-651193 (Counterclockwise)

Preceding gene: 148827320

Following gene: 148827318

Centisome position: 34.51

GC content: 38.74

Gene sequence:

>111_bases
ATGTCAGAACTTAATGAAATGCAAAAATTGGCGTGGGCTGGTTTTGCTGGTGGCGATTGGCAAGAAAATGTCAATGTACG
TGACTTTATCCAAAAAACTATACCCTTATGA

Upstream 100 bases:

>100_bases
AATATTGTCGGCGGGGCAATTTGTATTGGTGTATTCCAACGTTATTTAACCAAAACGCATTAAAGACTTACTTATTTATT
AATTAACAAAGGAAATGACT

Downstream 100 bases:

>100_bases
AGGCGATGACTCTTTCTTAGCAGGTCCAACCGAAGCAACAACCAAGCTTTGGGAATCTGTGATGGAAGGTATTAAAATTG
AAAACCGTACTCACGCGCCA

Product: formate acetyltransferase

Products: NA

Alternate protein names: Pyruvate formate-lyase [H]

Number of amino acids: Translated: 36; Mature: 35

Protein sequence:

>36_residues
MSELNEMQKLAWAGFAGGDWQENVNVRDFIQKTIPL

Sequences:

>Translated_36_residues
MSELNEMQKLAWAGFAGGDWQENVNVRDFIQKTIPL
>Mature_35_residues
SELNEMQKLAWAGFAGGDWQENVNVRDFIQKTIPL

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

None

Paralogues:

None

Copy number: 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005949
- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 4138; Mature: 4007

Theoretical pI: Translated: 4.26; Mature: 4.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELNEMQKLAWAGFAGGDWQENVNVRDFIQKTIPL
CCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCC
>Mature Secondary Structure 
SELNEMQKLAWAGFAGGDWQENVNVRDFIQKTIPL
CHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]