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The map label for this gene is pflB [H]

Identifier: 148827318

GI number: 148827318

Start: 649014

End: 651230

Strand: Reverse

Name: pflB [H]

Synonym: CGSHiGG_03495

Alternate gene names: 148827318

Gene position: 651230-649014 (Counterclockwise)

Preceding gene: 148827319

Following gene: 148827317

Centisome position: 34.51

GC content: 39.56

Gene sequence:

>2217_bases
ATGTACGTGACTTTATCCAAAAAACTATACCCTTATGAAGGCGATGACTCTTTCTTAGCAGGTCCAACCGAAGCAACAAC
CAAGCTTTGGGAATCTGTGATGGAAGGTATTAAAATTGAAAACCGTACTCACGCGCCATTAGATTTTGATGAACATACAC
CATCTACCATTATCTCTCACGCACCTGGTTACATTAACAAAGATTTAGAAAAAATCGTTGGTCTTCAAACTGATGAACCT
TTAAAACGTGCCATTATGCCATTCGGTGGTATCAAAATGGTGGAAGGTTCTTGTAAAGTTTATGGTCGTGAACTTGATCC
AAAAGTGAAAAAAATCTTCACTGAATACCGTAAAACACATAACCAAGGTGTATTCGATGTTTACACGCCAGATATTTTAC
GTTGCCGTAAATCTGGGGTATTAACTGGTCTTCCAGATGCTTATGGTCGTGGTCGTATCATCGGTGACTACCGTCGTGTA
GCACTTTATGGTGTAGATTTCTTAATGAAAGATAAATACGCACAATTCTCTTCTTTACAAAAAGATTTAGAAGATGGCGT
AAATCTTGAAGCAACAATTCGTTTACGTGAAGAAATCGCAGAACAACACCGTGCATTAGGTCAATTAAAACAAATGGCAG
CAAGCTATGGTTATGATATTTCTAACCCAGCAACTAATGCTCAAGAAGCCATTCAATGGATGTACTTTGCTTATCTTGCT
GCAATCAAATCACAAAATGGTGCTGCAATGTCATTCGGTCGTACCGCAACCTTTATTGACGTGTACATCGAACGTGATTT
AAAAGCAGGGAAAATTACTGAAACTGAAGCGCAAGAATTAGTTGACCACTTAGTTATGAAACTTCGTATGGTTCGTTTCT
TACGTACACCTGAATACGATCAATTATTCTCTGGTGACCCAATGTGGGCAACTGAAACCATCGCAGGTATGGGTTTAGAT
GGTCGTACATTAGTAACCAAAAATACATTCCGTATTTTACACACCCTTTACAACATGGGTACTTCCCCAGAGCCAAACTT
AACCATTCTTTGGTCTGAACAATTACCTGAAAACTTCAAACGTTTCTGTGCAAAAGTATCGATTGATACCTCATCAGTTC
AATACGAAAACGATGATTTAATGCGTCCAGACTTCAACAACGATGACTACGCAATCGCATGTTGTGTATCACCAATGATT
GTGGGTAAACAAATGCAATTCTTCGGTGCACGTGCAAACTTAGCGAAAACATTGTTATACGCAATCAACGGCGGTATTGA
TGAAAAATTAGGTATGCAAGTAGGTCCGAAAACTGCACCAATTACTGATGAAGTATTAGATTTTGATACAGTAATGACTC
GTATGGATAGTTTCATGGATTGGTTGGCAAAACAATATGTGACTGCCTTAAACGTAATCCACTATATGCACGATAAATAT
TCATACGAAGCCGCATTAATGGCATTACATGATCGTGATGTATACCGTACTATGGCTTGTGGTATCGCAGGTCTTTCTGT
TGCGGCTGACTCACTTTCAGCAATCAAATATGCGAAAGTTAAACCAGTTCGTGGCGACATCAAAGATAAAGATGGCAATG
TTGTTGCAACTAACGTAGCAATCGACTTTGAAATCGAAGGTGAATATCCACAATATGGTAACAATGATAACCGTGTTGAT
GACATCGCTTGTGACTTAGTTGAACGTTTTATGAAGAAAATTCAAAAACTTAAAACTTACCGCAATGCAGTGCCTACACA
ATCTGTATTAACCATTACTTCTAACGTAGTTTATGGTAAGAAAACGGGCAACACCCCTGATGGTCGTCGTGCTGGTGCAC
CATTCGGACCAGGTGCGAACCCAATGCACGGACGTGACCAAAAAGGTGCGGTAGCATCATTAACTTCTGTAGCTAAACTT
CCATTTGCTTACGCGAAAGATGGTATTTCTTATACCTTCTCAATCGTACCAAATGCCTTAGGTAAAGATGCCGAAGCACA
ACGTCGCAACCTTGCTGGCTTAATGGATGGTTACTTCCACCACGAAGCAACGGTTGAAGGTGGCCAACACTTAAATGTGA
ACGTGTTAAACCGTGAAATGTTGTTAGATGCAATGGAAAATCCGGATAAATATCCACAATTGACTATCCGTGTATCAGGT
TACGCAGTACGTTTTAACTCTTTAACTAAAAGAGCAACAACAAGACGTAATTACTAG

Upstream 100 bases:

>100_bases
ATTTATTAATTAACAAAGGAAATGACTATGTCAGAACTTAATGAAATGCAAAAATTGGCGTGGGCTGGTTTTGCTGGTGG
CGATTGGCAAGAAAATGTCA

Downstream 100 bases:

>100_bases
AACTTTCACAGAGTCAATGTAAAAACATGTTTATTTTTGACCGCACTTTGGCGGGAACATAATATGTATGAGCCTGATTT
AAATCAGGCTCATTTTTTAT

Product: formate acetyltransferase

Products: NA

Alternate protein names: Pyruvate formate-lyase [H]

Number of amino acids: Translated: 738; Mature: 738

Protein sequence:

>738_residues
MYVTLSKKLYPYEGDDSFLAGPTEATTKLWESVMEGIKIENRTHAPLDFDEHTPSTIISHAPGYINKDLEKIVGLQTDEP
LKRAIMPFGGIKMVEGSCKVYGRELDPKVKKIFTEYRKTHNQGVFDVYTPDILRCRKSGVLTGLPDAYGRGRIIGDYRRV
ALYGVDFLMKDKYAQFSSLQKDLEDGVNLEATIRLREEIAEQHRALGQLKQMAASYGYDISNPATNAQEAIQWMYFAYLA
AIKSQNGAAMSFGRTATFIDVYIERDLKAGKITETEAQELVDHLVMKLRMVRFLRTPEYDQLFSGDPMWATETIAGMGLD
GRTLVTKNTFRILHTLYNMGTSPEPNLTILWSEQLPENFKRFCAKVSIDTSSVQYENDDLMRPDFNNDDYAIACCVSPMI
VGKQMQFFGARANLAKTLLYAINGGIDEKLGMQVGPKTAPITDEVLDFDTVMTRMDSFMDWLAKQYVTALNVIHYMHDKY
SYEAALMALHDRDVYRTMACGIAGLSVAADSLSAIKYAKVKPVRGDIKDKDGNVVATNVAIDFEIEGEYPQYGNNDNRVD
DIACDLVERFMKKIQKLKTYRNAVPTQSVLTITSNVVYGKKTGNTPDGRRAGAPFGPGANPMHGRDQKGAVASLTSVAKL
PFAYAKDGISYTFSIVPNALGKDAEAQRRNLAGLMDGYFHHEATVEGGQHLNVNVLNREMLLDAMENPDKYPQLTIRVSG
YAVRFNSLTKRATTRRNY

Sequences:

>Translated_738_residues
MYVTLSKKLYPYEGDDSFLAGPTEATTKLWESVMEGIKIENRTHAPLDFDEHTPSTIISHAPGYINKDLEKIVGLQTDEP
LKRAIMPFGGIKMVEGSCKVYGRELDPKVKKIFTEYRKTHNQGVFDVYTPDILRCRKSGVLTGLPDAYGRGRIIGDYRRV
ALYGVDFLMKDKYAQFSSLQKDLEDGVNLEATIRLREEIAEQHRALGQLKQMAASYGYDISNPATNAQEAIQWMYFAYLA
AIKSQNGAAMSFGRTATFIDVYIERDLKAGKITETEAQELVDHLVMKLRMVRFLRTPEYDQLFSGDPMWATETIAGMGLD
GRTLVTKNTFRILHTLYNMGTSPEPNLTILWSEQLPENFKRFCAKVSIDTSSVQYENDDLMRPDFNNDDYAIACCVSPMI
VGKQMQFFGARANLAKTLLYAINGGIDEKLGMQVGPKTAPITDEVLDFDTVMTRMDSFMDWLAKQYVTALNVIHYMHDKY
SYEAALMALHDRDVYRTMACGIAGLSVAADSLSAIKYAKVKPVRGDIKDKDGNVVATNVAIDFEIEGEYPQYGNNDNRVD
DIACDLVERFMKKIQKLKTYRNAVPTQSVLTITSNVVYGKKTGNTPDGRRAGAPFGPGANPMHGRDQKGAVASLTSVAKL
PFAYAKDGISYTFSIVPNALGKDAEAQRRNLAGLMDGYFHHEATVEGGQHLNVNVLNREMLLDAMENPDKYPQLTIRVSG
YAVRFNSLTKRATTRRNY
>Mature_738_residues
MYVTLSKKLYPYEGDDSFLAGPTEATTKLWESVMEGIKIENRTHAPLDFDEHTPSTIISHAPGYINKDLEKIVGLQTDEP
LKRAIMPFGGIKMVEGSCKVYGRELDPKVKKIFTEYRKTHNQGVFDVYTPDILRCRKSGVLTGLPDAYGRGRIIGDYRRV
ALYGVDFLMKDKYAQFSSLQKDLEDGVNLEATIRLREEIAEQHRALGQLKQMAASYGYDISNPATNAQEAIQWMYFAYLA
AIKSQNGAAMSFGRTATFIDVYIERDLKAGKITETEAQELVDHLVMKLRMVRFLRTPEYDQLFSGDPMWATETIAGMGLD
GRTLVTKNTFRILHTLYNMGTSPEPNLTILWSEQLPENFKRFCAKVSIDTSSVQYENDDLMRPDFNNDDYAIACCVSPMI
VGKQMQFFGARANLAKTLLYAINGGIDEKLGMQVGPKTAPITDEVLDFDTVMTRMDSFMDWLAKQYVTALNVIHYMHDKY
SYEAALMALHDRDVYRTMACGIAGLSVAADSLSAIKYAKVKPVRGDIKDKDGNVVATNVAIDFEIEGEYPQYGNNDNRVD
DIACDLVERFMKKIQKLKTYRNAVPTQSVLTITSNVVYGKKTGNTPDGRRAGAPFGPGANPMHGRDQKGAVASLTSVAKL
PFAYAKDGISYTFSIVPNALGKDAEAQRRNLAGLMDGYFHHEATVEGGQHLNVNVLNREMLLDAMENPDKYPQLTIRVSG
YAVRFNSLTKRATTRRNY

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1787131, Length=726, Percent_Identity=84.9862258953168, Blast_Score=1310, Evalue=0.0,
Organism=Escherichia coli, GI48994926, Length=720, Percent_Identity=75.5555555555556, Blast_Score=1173, Evalue=0.0,
Organism=Escherichia coli, GI1787044, Length=577, Percent_Identity=27.209705372617, Blast_Score=176, Evalue=6e-45,
Organism=Escherichia coli, GI1790388, Length=534, Percent_Identity=25.0936329588015, Blast_Score=132, Evalue=1e-31,
Organism=Escherichia coli, GI1788933, Length=48, Percent_Identity=81.25, Blast_Score=84, Evalue=3e-17,

Paralogues:

None

Copy number: 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005949
- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 82678; Mature: 82678

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYVTLSKKLYPYEGDDSFLAGPTEATTKLWESVMEGIKIENRTHAPLDFDEHTPSTIISH
CEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCEECCCCCCCCCCCCCCCHHHHHC
APGYINKDLEKIVGLQTDEPLKRAIMPFGGIKMVEGSCKVYGRELDPKVKKIFTEYRKTH
CCCCCHHHHHHHHCCCCCCHHHHHHCCCCCEEEECCCCEEECCCCCHHHHHHHHHHHHHH
NQGVFDVYTPDILRCRKSGVLTGLPDAYGRGRIIGDYRRVALYGVDFLMKDKYAQFSSLQ
CCCEEEECCHHHHHHHHCCCCCCCCCCCCCCCEEHHHHEEHHHHHHHHHHHHHHHHHHHH
KDLEDGVNLEATIRLREEIAEQHRALGQLKQMAASYGYDISNPATNAQEAIQWMYFAYLA
HHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
AIKSQNGAAMSFGRTATFIDVYIERDLKAGKITETEAQELVDHLVMKLRMVRFLRTPEYD
HHHCCCCCEEECCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHH
QLFSGDPMWATETIAGMGLDGRTLVTKNTFRILHTLYNMGTSPEPNLTILWSEQLPENFK
HHCCCCCCCHHHHHHCCCCCCCEEEEHHHHHHHHHHHHCCCCCCCCEEEEECHHCCHHHH
RFCAKVSIDTSSVQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTLLY
HHHHHEECCCCCEEECCCCCCCCCCCCCCCEEEEECCHHHHCCHHHHHHHHHHHHHHHHH
AINGGIDEKLGMQVGPKTAPITDEVLDFDTVMTRMDSFMDWLAKQYVTALNVIHYMHDKY
HHCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SYEAALMALHDRDVYRTMACGIAGLSVAADSLSAIKYAKVKPVRGDIKDKDGNVVATNVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEE
IDFEIEGEYPQYGNNDNRVDDIACDLVERFMKKIQKLKTYRNAVPTQSVLTITSNVVYGK
EEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCEEEEC
KTGNTPDGRRAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNAL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHCCCCEEEEEECCHHC
GKDAEAQRRNLAGLMDGYFHHEATVEGGQHLNVNVLNREMLLDAMENPDKYPQLTIRVSG
CCCHHHHHHHHHHHHHHHHHCCCEECCCCEEEEEEHHHHHHHHHHCCCCCCCEEEEEEEC
YAVRFNSLTKRATTRRNY
EEEEEHHHHHHHHHCCCC
>Mature Secondary Structure
MYVTLSKKLYPYEGDDSFLAGPTEATTKLWESVMEGIKIENRTHAPLDFDEHTPSTIISH
CEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCEECCCCCCCCCCCCCCCHHHHHC
APGYINKDLEKIVGLQTDEPLKRAIMPFGGIKMVEGSCKVYGRELDPKVKKIFTEYRKTH
CCCCCHHHHHHHHCCCCCCHHHHHHCCCCCEEEECCCCEEECCCCCHHHHHHHHHHHHHH
NQGVFDVYTPDILRCRKSGVLTGLPDAYGRGRIIGDYRRVALYGVDFLMKDKYAQFSSLQ
CCCEEEECCHHHHHHHHCCCCCCCCCCCCCCCEEHHHHEEHHHHHHHHHHHHHHHHHHHH
KDLEDGVNLEATIRLREEIAEQHRALGQLKQMAASYGYDISNPATNAQEAIQWMYFAYLA
HHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
AIKSQNGAAMSFGRTATFIDVYIERDLKAGKITETEAQELVDHLVMKLRMVRFLRTPEYD
HHHCCCCCEEECCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHH
QLFSGDPMWATETIAGMGLDGRTLVTKNTFRILHTLYNMGTSPEPNLTILWSEQLPENFK
HHCCCCCCCHHHHHHCCCCCCCEEEEHHHHHHHHHHHHCCCCCCCCEEEEECHHCCHHHH
RFCAKVSIDTSSVQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTLLY
HHHHHEECCCCCEEECCCCCCCCCCCCCCCEEEEECCHHHHCCHHHHHHHHHHHHHHHHH
AINGGIDEKLGMQVGPKTAPITDEVLDFDTVMTRMDSFMDWLAKQYVTALNVIHYMHDKY
HHCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SYEAALMALHDRDVYRTMACGIAGLSVAADSLSAIKYAKVKPVRGDIKDKDGNVVATNVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEE
IDFEIEGEYPQYGNNDNRVDDIACDLVERFMKKIQKLKTYRNAVPTQSVLTITSNVVYGK
EEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCEEEEC
KTGNTPDGRRAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNAL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHCCCCEEEEEECCHHC
GKDAEAQRRNLAGLMDGYFHHEATVEGGQHLNVNVLNREMLLDAMENPDKYPQLTIRVSG
CCCHHHHHHHHHHHHHHHHHCCCEECCCCEEEEEEHHHHHHHHHHCCCCCCCEEEEEEEC
YAVRFNSLTKRATTRRNY
EEEEEHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]