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The map label for this gene is focA

Identifier: 148827320

GI number: 148827320

Start: 651341

End: 652195

Strand: Reverse

Name: focA

Synonym: CGSHiGG_03505

Alternate gene names: 148827320

Gene position: 652195-651341 (Counterclockwise)

Preceding gene: 148827323

Following gene: 148827319

Centisome position: 34.56

GC content: 38.71

Gene sequence:

>855_bases
ATGGCATCAGAAAATCAAAAACTATCTTCTGTTGCACTCACACCTGTTGAAGCAACGGATTATGCAGAAAATACAGCAAC
TTATAAAGCAAATAAACGTCCATTTTTATCGTTTATGTCCGGTATTAGTGCTGGTGCTTGTATTGCCTTAGCCTTTGTAT
TTTACACGACAACACAAACTGCTAGTGCAGGCGCACCTTGGGGATTAACCAAGTTAGTAGGTGGATTAGTCTTCTCCTTG
GGCGTAATTATGGTGGTGATTTTAGGATCTGAATTATTCACTTCTTCTACTTTAACTTTAGTCGCCCGTGTAGGCGGTAG
AATCACCACGACACAAATGATTCGAAATTGGATCGTGGTGTATTTAGGCAACTTCGTTGGTGGTTTATTTATTGCGGCTG
TTATTTGGTTTAGTGGACAAACAATGGCAGCAAATGGTCAATGGGGTTTAACAATTCTTGCAACCGCCCAACATAAAATT
CATCACACTTGGTTTGAAGCTTTTAACCTAGGTATTTTATGTAACATTATGGTGTGTGTGGCGGTTTGGATGTCTTATTC
TGGTAAGACTGTTACAGACAAAGCATTTATTATGATTATGCCGATTGGTTTGTTTGTCGCGTCAGGATTTGAACACTGTG
TGGCTAATATGTTTATGATTCCGATGGGAATTATTACCGCACATTTTAGTACACCTGAATTTTGGCAACAAATCGGTGTT
GATCCAATGAAATACGCAGATTTAGATTTGTACCATTTCATCGTGAAGAATTTAATCCCTGTAACGTTGGGAAATATTGT
CGGCGGGGCAATTTGTATTGGTGTATTCCAACGTTATTTAACCAAAACGCATTAA

Upstream 100 bases:

>100_bases
AGTAAAATAGCACCAATTATAGAAGTATTTGTAGTCGTAAACTTGTTAAAAGTATTAAATTTTCATCAACTTTTTCAATG
TCAATAGTTGGAGTTTGATT

Downstream 100 bases:

>100_bases
AGACTTACTTATTTATTAATTAACAAAGGAAATGACTATGTCAGAACTTAATGAAATGCAAAAATTGGCGTGGGCTGGTT
TTGCTGGTGGCGATTGGCAA

Product: putative formate transporter

Products: formate [Periplasm]; formate [Cytoplasm] [C]

Alternate protein names: Formate channel

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MASENQKLSSVALTPVEATDYAENTATYKANKRPFLSFMSGISAGACIALAFVFYTTTQTASAGAPWGLTKLVGGLVFSL
GVIMVVILGSELFTSSTLTLVARVGGRITTTQMIRNWIVVYLGNFVGGLFIAAVIWFSGQTMAANGQWGLTILATAQHKI
HHTWFEAFNLGILCNIMVCVAVWMSYSGKTVTDKAFIMIMPIGLFVASGFEHCVANMFMIPMGIITAHFSTPEFWQQIGV
DPMKYADLDLYHFIVKNLIPVTLGNIVGGAICIGVFQRYLTKTH

Sequences:

>Translated_284_residues
MASENQKLSSVALTPVEATDYAENTATYKANKRPFLSFMSGISAGACIALAFVFYTTTQTASAGAPWGLTKLVGGLVFSL
GVIMVVILGSELFTSSTLTLVARVGGRITTTQMIRNWIVVYLGNFVGGLFIAAVIWFSGQTMAANGQWGLTILATAQHKI
HHTWFEAFNLGILCNIMVCVAVWMSYSGKTVTDKAFIMIMPIGLFVASGFEHCVANMFMIPMGIITAHFSTPEFWQQIGV
DPMKYADLDLYHFIVKNLIPVTLGNIVGGAICIGVFQRYLTKTH
>Mature_283_residues
ASENQKLSSVALTPVEATDYAENTATYKANKRPFLSFMSGISAGACIALAFVFYTTTQTASAGAPWGLTKLVGGLVFSLG
VIMVVILGSELFTSSTLTLVARVGGRITTTQMIRNWIVVYLGNFVGGLFIAAVIWFSGQTMAANGQWGLTILATAQHKIH
HTWFEAFNLGILCNIMVCVAVWMSYSGKTVTDKAFIMIMPIGLFVASGFEHCVANMFMIPMGIITAHFSTPEFWQQIGVD
PMKYADLDLYHFIVKNLIPVTLGNIVGGAICIGVFQRYLTKTH

Specific function: Involved in the bidirectional transport of formate

COG id: COG2116

COG function: function code P; Formate/nitrite family of transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FNT transporter (TC 2.A.44) family

Homologues:

Organism=Escherichia coli, GI1787132, Length=263, Percent_Identity=53.2319391634981, Blast_Score=305, Evalue=2e-84,
Organism=Escherichia coli, GI1788837, Length=274, Percent_Identity=42.7007299270073, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI87082249, Length=213, Percent_Identity=32.3943661971831, Blast_Score=73, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6321779, Length=240, Percent_Identity=28.75, Blast_Score=86, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOCA_HAEIN (P43756)

Other databases:

- EMBL:   L42023
- PIR:   G64052
- RefSeq:   NP_438349.1
- GeneID:   951090
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0181
- TIGR:   HI_0181
- HOGENOM:   HBG726755
- OMA:   ANGGWGL
- ProtClustDB:   CLSK869952
- BioCyc:   HINF71421:HI_0181-MONOMER
- InterPro:   IPR000292
- TIGRFAMs:   TIGR00790

Pfam domain/function: PF01226 Form_Nir_trans

EC number: NA

Molecular weight: Translated: 30851; Mature: 30719

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: PS01005 FORMATE_NITRITE_TP_1; PS01006 FORMATE_NITRITE_TP_2

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1204fac8)-; HASH(0x11f3abb8)-; HASH(0x11f672a8)-; HASH(0x11ac7590)-; HASH(0x11a3c6ac)-; HASH(0x11b10018)-;

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASENQKLSSVALTPVEATDYAENTATYKANKRPFLSFMSGISAGACIALAFVFYTTTQT
CCCCCCCHHHEEECCCCHHCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCC
ASAGAPWGLTKLVGGLVFSLGVIMVVILGSELFTSSTLTLVARVGGRITTTQMIRNWIVV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
YLGNFVGGLFIAAVIWFSGQTMAANGQWGLTILATAQHKIHHTWFEAFNLGILCNIMVCV
HHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHH
AVWMSYSGKTVTDKAFIMIMPIGLFVASGFEHCVANMFMIPMGIITAHFSTPEFWQQIGV
HHHHHCCCCEECCCCEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCC
DPMKYADLDLYHFIVKNLIPVTLGNIVGGAICIGVFQRYLTKTH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
>Mature Secondary Structure 
ASENQKLSSVALTPVEATDYAENTATYKANKRPFLSFMSGISAGACIALAFVFYTTTQT
CCCCCCHHHEEECCCCHHCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCC
ASAGAPWGLTKLVGGLVFSLGVIMVVILGSELFTSSTLTLVARVGGRITTTQMIRNWIVV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
YLGNFVGGLFIAAVIWFSGQTMAANGQWGLTILATAQHKIHHTWFEAFNLGILCNIMVCV
HHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHH
AVWMSYSGKTVTDKAFIMIMPIGLFVASGFEHCVANMFMIPMGIITAHFSTPEFWQQIGV
HHHHHCCCCEECCCCEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCC
DPMKYADLDLYHFIVKNLIPVTLGNIVGGAICIGVFQRYLTKTH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate [Cytoplasm]; formate [Periplasm] [C]

Specific reaction: formate [Cytoplasm] = formate [Periplasm] formate [Periplasm] = formate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800