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The map label for this gene is pflA [H]

Identifier: 148827317

GI number: 148827317

Start: 648121

End: 648861

Strand: Reverse

Name: pflA [H]

Synonym: CGSHiGG_03490

Alternate gene names: 148827317

Gene position: 648861-648121 (Counterclockwise)

Preceding gene: 148827318

Following gene: 148827316

Centisome position: 34.38

GC content: 36.84

Gene sequence:

>741_bases
ATGTCAGTTCTTGGACGAATTCACTCTTTTGAATCCTGTGGCACTGTAGATGGGCCAGGTATTCGTTTTATTTTATTTAT
GCAAGGCTGCTTGATGCGCTGCAAATATTGCCACAATCGTGATACTTGGGATCTTGAAGGTGGTAAAGAAATCAGTGTCG
AAGATTTAATGAAAGAAGTCGTGACTTATCGCCATTTTATGAATGCTACTGGCGGTGGTGTCACAGCATCTGGTGGCGAG
GCTGTGTTACAAGCAGAGTTTGTACGCGATTGGTTCCGTGCTTGTAAAGAGGAAGGGATTAATACTTGCTTAGATACAAA
TGGTTTTGTACGTCATTATGATCATATTATTGATGAATTATTAGATGTAACAGATCTTGTTTTACTCGATTTAAAAGAGC
TTAATGATCAAGTTCATCAAAATCTTATTGGGGTACCAAATAAACGTACCCTTGAATTTGCAAAATATTTGCAAAAACGT
AATCAACATACTTGGATTCGTTATGTTGTGGTTCCTGGTTATACTGATAGCGATCACGATGTGCATTTATTAGGTCAGTT
TATTGAAGGTATGACCAATATTGAAAAAGTTGAACTTCTTCCTTATCATCGATTAGGTGCACATAAATGGAAAACCCTTG
GGTTAGATTATGAGCTTGAAGATGTATTACCGCCAACTAAAGAATCGTTAGAGCATATTAAAACAATTCTAGAAGGTTAT
GGACACACTGTAAAATTCTAG

Upstream 100 bases:

>100_bases
GGGAACATAATATGTATGAGCCTGATTTAAATCAGGCTCATTTTTTATAGACTAAATATTTGGTAAAATTTAGCTATATC
TAATTTTAGGAAATTTATGT

Downstream 100 bases:

>100_bases
AATAAATGTCAGCTAACATAAGGAGTAAATAATGAAAAAATTATTTTAACATTATCACTTGGGTTACTTACCGCCTGTTC
TGCTCAAACTCAAAAGGCTG

Product: pyruvate formate lyase-activating enzyme 1

Products: NA

Alternate protein names: Formate-C-acetyltransferase-activating enzyme 1; PFL-activating enzyme 1 [H]

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MSVLGRIHSFESCGTVDGPGIRFILFMQGCLMRCKYCHNRDTWDLEGGKEISVEDLMKEVVTYRHFMNATGGGVTASGGE
AVLQAEFVRDWFRACKEEGINTCLDTNGFVRHYDHIIDELLDVTDLVLLDLKELNDQVHQNLIGVPNKRTLEFAKYLQKR
NQHTWIRYVVVPGYTDSDHDVHLLGQFIEGMTNIEKVELLPYHRLGAHKWKTLGLDYELEDVLPPTKESLEHIKTILEGY
GHTVKF

Sequences:

>Translated_246_residues
MSVLGRIHSFESCGTVDGPGIRFILFMQGCLMRCKYCHNRDTWDLEGGKEISVEDLMKEVVTYRHFMNATGGGVTASGGE
AVLQAEFVRDWFRACKEEGINTCLDTNGFVRHYDHIIDELLDVTDLVLLDLKELNDQVHQNLIGVPNKRTLEFAKYLQKR
NQHTWIRYVVVPGYTDSDHDVHLLGQFIEGMTNIEKVELLPYHRLGAHKWKTLGLDYELEDVLPPTKESLEHIKTILEGY
GHTVKF
>Mature_245_residues
SVLGRIHSFESCGTVDGPGIRFILFMQGCLMRCKYCHNRDTWDLEGGKEISVEDLMKEVVTYRHFMNATGGGVTASGGEA
VLQAEFVRDWFRACKEEGINTCLDTNGFVRHYDHIIDELLDVTDLVLLDLKELNDQVHQNLIGVPNKRTLEFAKYLQKRN
QHTWIRYVVVPGYTDSDHDVHLLGQFIEGMTNIEKVELLPYHRLGAHKWKTLGLDYELEDVLPPTKESLEHIKTILEGYG
HTVKF

Specific function: Activation of pyruvate formate-lyase 1 under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1787130, Length=246, Percent_Identity=73.9837398373984, Blast_Score=397, Evalue=1e-112,
Organism=Escherichia coli, GI1790389, Length=274, Percent_Identity=25.9124087591241, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI1790839, Length=272, Percent_Identity=28.3088235294118, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012838
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 28209; Mature: 28077

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVLGRIHSFESCGTVDGPGIRFILFMQGCLMRCKYCHNRDTWDLEGGKEISVEDLMKEV
CCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHH
VTYRHFMNATGGGVTASGGEAVLQAEFVRDWFRACKEEGINTCLDTNGFVRHYDHIIDEL
HHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHH
LDVTDLVLLDLKELNDQVHQNLIGVPNKRTLEFAKYLQKRNQHTWIRYVVVPGYTDSDHD
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCH
VHLLGQFIEGMTNIEKVELLPYHRLGAHKWKTLGLDYELEDVLPPTKESLEHIKTILEGY
HHHHHHHHHHHCCHHHHHCCCCHHHCCCHHHHCCCCEEHHHCCCCCHHHHHHHHHHHHHC
GHTVKF
CCCCCC
>Mature Secondary Structure 
SVLGRIHSFESCGTVDGPGIRFILFMQGCLMRCKYCHNRDTWDLEGGKEISVEDLMKEV
CHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHH
VTYRHFMNATGGGVTASGGEAVLQAEFVRDWFRACKEEGINTCLDTNGFVRHYDHIIDEL
HHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHH
LDVTDLVLLDLKELNDQVHQNLIGVPNKRTLEFAKYLQKRNQHTWIRYVVVPGYTDSDHD
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCH
VHLLGQFIEGMTNIEKVELLPYHRLGAHKWKTLGLDYELEDVLPPTKESLEHIKTILEGY
HHHHHHHHHHHCCHHHHHCCCCHHHCCCHHHHCCCCEEHHHCCCCCHHHHHHHHHHHHHC
GHTVKF
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]