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The map label for this gene is queF [H]

Identifier: 148827012

GI number: 148827012

Start: 253760

End: 254599

Strand: Direct

Name: queF [H]

Synonym: CGSHiGG_01500

Alternate gene names: 148827012

Gene position: 253760-254599 (Clockwise)

Preceding gene: 148827011

Following gene: 148827015

Centisome position: 13.45

GC content: 38.1

Gene sequence:

>840_bases
ATGAATTACCAAGACAACAGCTTAAAATCCTTAAAACTAGGTCAAAAAACGGAATACGCCTCTCAATATGACCGCACTTT
ATTACAGCCTGTGCCACGTGCGTTAAATCGCGATGGATTGGGCATTACTCAAAACCAACCATTTACTATCGGCGCCGATA
TTTGGACGGCTTATGAAATCTCGTGGCTCAACGAAAAAGGCTTACCGCAAGTGGCTATCGCAGACATTTATCTGGATTAT
CAAAGCCAAAATTTGATTGAATCAAAAAGTTTTAAACTTTATTTAAATAGCTTTAACCAAAGCAAATTTACCGACTTCAA
TGCCGTTCAACAAACTATGCAACGCGATTTAATCGAATGTGCGCAAGGCGATGTAAAAGTGCGGTTAAATCCAGTGGCAG
TTTATGATGCTCAAAAAATTGAGCATTTACAAGGCGATTGCATTGATGAACAAGATATTGAAATTACTAGCTACGAATTT
AACGCTAATTGGCTGAAAGATTGCGTATCGGATGAAATTGTCGAAGAAAAATTAGTCAGCCATTTATTGAAATCTAACTG
CCTCATTACCAATCAGCCAGACTGGGGTACCTTGCATATTCACTATGTTGGTAAAAAAATCAATCACGAAAAATTATTGC
GTTACGTTGTATCGTTCCGTCAGCATAATGAATTCCACGAACAATGTGTCGAACGTATTTTTTGCGATTTAATGCACTAC
GCAAAACCAGAAAAACTCACCGTTTACGCACGCTATACTCGTCGTGGCGGATTAGACATTAATCCATTCCGCTCTAATTT
TGAGAATTTACCTGAAAATTTAAGATTGGCGAGACAGTAA

Upstream 100 bases:

>100_bases
TGGAATGATAAAGTCTTAGCCCCCTTCCGACGCTTCGCGCCACCTTCCCCCCGCAAGCAAGGGAAGGCAAATTATTGTTT
AACATATAAAGGAAAAATAA

Downstream 100 bases:

>100_bases
AAAACTGTGGTGAAATTTCACCGCACTTTTCTCTACCCTTGTTTCAAATCATTTGCCTGTTGCAATAGCTGACGGCTTTC
TTTTAAGAATTGTTCGGAAT

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase [H]

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MNYQDNSLKSLKLGQKTEYASQYDRTLLQPVPRALNRDGLGITQNQPFTIGADIWTAYEISWLNEKGLPQVAIADIYLDY
QSQNLIESKSFKLYLNSFNQSKFTDFNAVQQTMQRDLIECAQGDVKVRLNPVAVYDAQKIEHLQGDCIDEQDIEITSYEF
NANWLKDCVSDEIVEEKLVSHLLKSNCLITNQPDWGTLHIHYVGKKINHEKLLRYVVSFRQHNEFHEQCVERIFCDLMHY
AKPEKLTVYARYTRRGGLDINPFRSNFENLPENLRLARQ

Sequences:

>Translated_279_residues
MNYQDNSLKSLKLGQKTEYASQYDRTLLQPVPRALNRDGLGITQNQPFTIGADIWTAYEISWLNEKGLPQVAIADIYLDY
QSQNLIESKSFKLYLNSFNQSKFTDFNAVQQTMQRDLIECAQGDVKVRLNPVAVYDAQKIEHLQGDCIDEQDIEITSYEF
NANWLKDCVSDEIVEEKLVSHLLKSNCLITNQPDWGTLHIHYVGKKINHEKLLRYVVSFRQHNEFHEQCVERIFCDLMHY
AKPEKLTVYARYTRRGGLDINPFRSNFENLPENLRLARQ
>Mature_279_residues
MNYQDNSLKSLKLGQKTEYASQYDRTLLQPVPRALNRDGLGITQNQPFTIGADIWTAYEISWLNEKGLPQVAIADIYLDY
QSQNLIESKSFKLYLNSFNQSKFTDFNAVQQTMQRDLIECAQGDVKVRLNPVAVYDAQKIEHLQGDCIDEQDIEITSYEF
NANWLKDCVSDEIVEEKLVSHLLKSNCLITNQPDWGTLHIHYVGKKINHEKLLRYVVSFRQHNEFHEQCVERIFCDLMHY
AKPEKLTVYARYTRRGGLDINPFRSNFENLPENLRLARQ

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) [H]

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789158, Length=275, Percent_Identity=61.4545454545455, Blast_Score=348, Evalue=3e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016428
- InterPro:   IPR020602 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =1.7.1.13 [H]

Molecular weight: Translated: 32574; Mature: 32574

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYQDNSLKSLKLGQKTEYASQYDRTLLQPVPRALNRDGLGITQNQPFTIGADIWTAYEI
CCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCEEEEEE
SWLNEKGLPQVAIADIYLDYQSQNLIESKSFKLYLNSFNQSKFTDFNAVQQTMQRDLIEC
EECCCCCCCCEEEEEEEEECCCCCCHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHH
AQGDVKVRLNPVAVYDAQKIEHLQGDCIDEQDIEITSYEFNANWLKDCVSDEIVEEKLVS
CCCCEEEEEEEEEEECHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHH
HLLKSNCLITNQPDWGTLHIHYVGKKINHEKLLRYVVSFRQHNEFHEQCVERIFCDLMHY
HHHHCCCEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
AKPEKLTVYARYTRRGGLDINPFRSNFENLPENLRLARQ
CCCCEEEEEEEEECCCCCCCCHHHHHHHHCCHHHHHCCC
>Mature Secondary Structure
MNYQDNSLKSLKLGQKTEYASQYDRTLLQPVPRALNRDGLGITQNQPFTIGADIWTAYEI
CCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCEEEEEE
SWLNEKGLPQVAIADIYLDYQSQNLIESKSFKLYLNSFNQSKFTDFNAVQQTMQRDLIEC
EECCCCCCCCEEEEEEEEECCCCCCHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHH
AQGDVKVRLNPVAVYDAQKIEHLQGDCIDEQDIEITSYEFNANWLKDCVSDEIVEEKLVS
CCCCEEEEEEEEEEECHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHH
HLLKSNCLITNQPDWGTLHIHYVGKKINHEKLLRYVVSFRQHNEFHEQCVERIFCDLMHY
HHHHCCCEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
AKPEKLTVYARYTRRGGLDINPFRSNFENLPENLRLARQ
CCCCEEEEEEEEECCCCCCCCHHHHHHHHCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA