Click here to switch to the map view.

The map label for this gene is dnaA [H]

Identifier: 148826359

GI number: 148826359

Start: 1389072

End: 1390436

Strand: Direct

Name: dnaA [H]

Synonym: CGSHiEE_07000

Alternate gene names: 148826359

Gene position: 1389072-1390436 (Clockwise)

Preceding gene: 148826358

Following gene: 148826360

Centisome position: 76.62

GC content: 39.63

Gene sequence:

>1365_bases
GTGAATCTCTCAAATCTTTGGCAAAGTTGCCTGTTACAACTTCAAGATCAAGTTTCAGCCAGCGATCTTAGCACTTGGCT
TCGTCCTTTACAGGCTGATGTGGTGGCGGATAACCACATTGTTTTATATGCTTCGAATATGTTTGTGAAAGGTTGGGTGG
AAACCCATTATCTCGCACAAATCCAGCAAATTTGTCAAACACTTGCACAAAATCCTGAACTGCGTATTTCTTTAAAAGAA
GGGGTTAAACCTGCACCCAAGATAGTGGAAAGTACACCAAATACAACACTTAGATCAGAAAGTGCGGTAGATTTTCAAAC
AGAATCTAATACGCCAATAAAATTTGAATCCCATCTCAATACTAAACATTTATTTGATAACTTTGTTGAAGGTAAATCGA
ACCAACTCGCACGTGCAGTCGGTCAAAAACTTGCTCAAGCACCAGGCGAACCATCAGCCAATCCTTTCTTTTTATATGGC
GGCACTGGTTTAGGGAAAACTCACTTGTTACACGCCATTGGCAACGGCATTTTAGCCGATAAACCGAATGCGCGAGTGCT
TTATATTCACGCCAACAATTTTATGCAACATATGGTAAAAGCAGTGCGTGATAATAAAATGGATCAATTTAAAAAATTCT
ATCGTTCTCTTGATGCGCTTTTAGTGGATGACATTCAATTTTTCGCTGAAAAAGAAAAAACTCAAGAAGAATTCTTCCAT
ATTTTCAATAGCTTGTTTGAAACGGGTCGCCAAATTATCTTAACTTCAGATCGTTATCCTAAAGAAATTGAAAAAATTGA
AGAACGTTTAAAATCACGTTTTGGCTGGGGCTTAACAACAGCCATTGAGCCGCCCGATCTTGAAACCCGTGTAGCGATTT
TATTGAAAAAAGCGGAAGAACATAATATGAACTTGCCAGAAGAAGTCGCTTTCTTCATTGCTCAACGCTTGCGTACCAAC
GTGCGTGAACTTGAGGGTGCATTAAATCGTGTAAAAGCAATGCAAGACTTCAAAGGCGGTGACATTGATATTGATTTCGT
GCGTGATACCTTAAAAGATATTTTGGCTCTGCAAGAACGCTTGGTTACCATTGAAAATATTCAAAAAGTGGTGGCTGAAT
ATTATCGAATTAAGGTTTCAGATTTAAAATCAAAAAGTCGCGCACGTTCAGTGACTCGTCCTCGCCAAATTGCTATGGCG
TTAGCAAAAGAATTAACAAATCGCAGTTTGCCTGAAATCGGTCGTGCCTTTGACCGTGATCACACGACCGTATTAAACGC
TTGCCGAGAAGTACCAAAATTCCGTGAGCAAGACAATAGCATTCAAGAAGATTGGGCGAATTTGATCCGCACTTTATCCG
CATAA

Upstream 100 bases:

>100_bases
AACTTGGATTTTTTAGAAGATCTTTCCACGCATTTATTGTAAAATCTCCGACAATTTTTACCGCACTTTTCTCTATTACA
AAAACAATAAGGATCCTTTT

Downstream 100 bases:

>100_bases
AAGGAGAACGTAATGCAATTTAGTATTTCAAGAGAAAATTTATTAAAACCGTTGCAACAAGTATGTGGCGTATTGAGTCA
TCGCCCAAATATTCCTGTAT

Product: chromosomal replication initiation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MNLSNLWQSCLLQLQDQVSASDLSTWLRPLQADVVADNHIVLYASNMFVKGWVETHYLAQIQQICQTLAQNPELRISLKE
GVKPAPKIVESTPNTTLRSESAVDFQTESNTPIKFESHLNTKHLFDNFVEGKSNQLARAVGQKLAQAPGEPSANPFFLYG
GTGLGKTHLLHAIGNGILADKPNARVLYIHANNFMQHMVKAVRDNKMDQFKKFYRSLDALLVDDIQFFAEKEKTQEEFFH
IFNSLFETGRQIILTSDRYPKEIEKIEERLKSRFGWGLTTAIEPPDLETRVAILLKKAEEHNMNLPEEVAFFIAQRLRTN
VRELEGALNRVKAMQDFKGGDIDIDFVRDTLKDILALQERLVTIENIQKVVAEYYRIKVSDLKSKSRARSVTRPRQIAMA
LAKELTNRSLPEIGRAFDRDHTTVLNACREVPKFREQDNSIQEDWANLIRTLSA

Sequences:

>Translated_454_residues
MNLSNLWQSCLLQLQDQVSASDLSTWLRPLQADVVADNHIVLYASNMFVKGWVETHYLAQIQQICQTLAQNPELRISLKE
GVKPAPKIVESTPNTTLRSESAVDFQTESNTPIKFESHLNTKHLFDNFVEGKSNQLARAVGQKLAQAPGEPSANPFFLYG
GTGLGKTHLLHAIGNGILADKPNARVLYIHANNFMQHMVKAVRDNKMDQFKKFYRSLDALLVDDIQFFAEKEKTQEEFFH
IFNSLFETGRQIILTSDRYPKEIEKIEERLKSRFGWGLTTAIEPPDLETRVAILLKKAEEHNMNLPEEVAFFIAQRLRTN
VRELEGALNRVKAMQDFKGGDIDIDFVRDTLKDILALQERLVTIENIQKVVAEYYRIKVSDLKSKSRARSVTRPRQIAMA
LAKELTNRSLPEIGRAFDRDHTTVLNACREVPKFREQDNSIQEDWANLIRTLSA
>Mature_454_residues
MNLSNLWQSCLLQLQDQVSASDLSTWLRPLQADVVADNHIVLYASNMFVKGWVETHYLAQIQQICQTLAQNPELRISLKE
GVKPAPKIVESTPNTTLRSESAVDFQTESNTPIKFESHLNTKHLFDNFVEGKSNQLARAVGQKLAQAPGEPSANPFFLYG
GTGLGKTHLLHAIGNGILADKPNARVLYIHANNFMQHMVKAVRDNKMDQFKKFYRSLDALLVDDIQFFAEKEKTQEEFFH
IFNSLFETGRQIILTSDRYPKEIEKIEERLKSRFGWGLTTAIEPPDLETRVAILLKKAEEHNMNLPEEVAFFIAQRLRTN
VRELEGALNRVKAMQDFKGGDIDIDFVRDTLKDILALQERLVTIENIQKVVAEYYRIKVSDLKSKSRARSVTRPRQIAMA
LAKELTNRSLPEIGRAFDRDHTTVLNACREVPKFREQDNSIQEDWANLIRTLSA

Specific function: Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box):5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phosph

COG id: COG0593

COG function: function code L; ATPase involved in DNA replication initiation

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dnaA family [H]

Homologues:

Organism=Escherichia coli, GI2367267, Length=476, Percent_Identity=59.453781512605, Blast_Score=561, Evalue=1e-161,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001957
- InterPro:   IPR020591
- InterPro:   IPR018312
- InterPro:   IPR013159
- InterPro:   IPR013317
- InterPro:   IPR010921 [H]

Pfam domain/function: PF00308 Bac_DnaA; PF08299 Bac_DnaA_C [H]

EC number: NA

Molecular weight: Translated: 51850; Mature: 51850

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: PS01008 DNAA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLSNLWQSCLLQLQDQVSASDLSTWLRPLQADVVADNHIVLYASNMFVKGWVETHYLAQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCEEEEEECCEEEEHHHHHHHHHH
IQQICQTLAQNPELRISLKEGVKPAPKIVESTPNTTLRSESAVDFQTESNTPIKFESHLN
HHHHHHHHHCCCCEEEEHHHCCCCCHHHHHCCCCCCCCCCCCCEEECCCCCCEEECCCCC
TKHLFDNFVEGKSNQLARAVGQKLAQAPGEPSANPFFLYGGTGLGKTHLLHAIGNGILAD
HHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCC
KPNARVLYIHANNFMQHMVKAVRDNKMDQFKKFYRSLDALLVDDIQFFAEKEKTQEEFFH
CCCCEEEEEECCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IFNSLFETGRQIILTSDRYPKEIEKIEERLKSRFGWGLTTAIEPPDLETRVAILLKKAEE
HHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHH
HNMNLPEEVAFFIAQRLRTNVRELEGALNRVKAMQDFKGGDIDIDFVRDTLKDILALQER
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHH
LVTIENIQKVVAEYYRIKVSDLKSKSRARSVTRPRQIAMALAKELTNRSLPEIGRAFDRD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHCCC
HTTVLNACREVPKFREQDNSIQEDWANLIRTLSA
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MNLSNLWQSCLLQLQDQVSASDLSTWLRPLQADVVADNHIVLYASNMFVKGWVETHYLAQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCEEEEEECCEEEEHHHHHHHHHH
IQQICQTLAQNPELRISLKEGVKPAPKIVESTPNTTLRSESAVDFQTESNTPIKFESHLN
HHHHHHHHHCCCCEEEEHHHCCCCCHHHHHCCCCCCCCCCCCCEEECCCCCCEEECCCCC
TKHLFDNFVEGKSNQLARAVGQKLAQAPGEPSANPFFLYGGTGLGKTHLLHAIGNGILAD
HHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCC
KPNARVLYIHANNFMQHMVKAVRDNKMDQFKKFYRSLDALLVDDIQFFAEKEKTQEEFFH
CCCCEEEEEECCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IFNSLFETGRQIILTSDRYPKEIEKIEERLKSRFGWGLTTAIEPPDLETRVAILLKKAEE
HHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHH
HNMNLPEEVAFFIAQRLRTNVRELEGALNRVKAMQDFKGGDIDIDFVRDTLKDILALQER
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHH
LVTIENIQKVVAEYYRIKVSDLKSKSRARSVTRPRQIAMALAKELTNRSLPEIGRAFDRD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHCCC
HTTVLNACREVPKFREQDNSIQEDWANLIRTLSA
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]