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The map label for this gene is folE [H]

Identifier: 148825991

GI number: 148825991

Start: 989485

End: 990699

Strand: Direct

Name: folE [H]

Synonym: CGSHiEE_04840

Alternate gene names: 148825991

Gene position: 989485-990699 (Clockwise)

Preceding gene: 148825987

Following gene: 148825992

Centisome position: 54.58

GC content: 40.08

Gene sequence:

>1215_bases
ATGCTTACATTGGATCAAGCTCGCTCGAAAATGCTTGAACAGCTTCCCTTCCCGACTCAAACTGAATACCTAAATTTACA
AGAAGCCGCCAATCGAATTTGTGCAGAAGATATTATTTCGCCTATCAATGTTCCCTCTTTCGATAACTCAGCAATGGATG
GTTATGCTGTACGTTTATCGGATTTACAACAATCTTTAACCCTTTCAGTGGCAGGGAAATCTTTTGCAGGTAATCCATTC
AAAGAGGAATGGCCATCAAAAAGTGCGGTCAGAATTATGACTGGCGCGATGATTCCTGAAGGCACAGATGCAGTGATAAT
GCAAGAGCAAGTAACACTCAATGAAGACGGCACCATCACTTTTAGTGAATTACCTAAACCAAATCAAAACATCCGTCGTA
TTGGTGAAGATGTAAAAAAAGGGGATGTTGTATTAGAACAAGGCGCACCGCTCACCCCAGTTTCCTTGCCATTATTAGCC
TCTCTAGGCATTGCAGAAGTAAAATGCTATCGTCAATTAAAAGTCGGTGTACTCTCAACTGGCGATGAACTTGTGGAAGT
CGGCAAGCCATTACAAAGCGGACAAATATATGACACTAACCGTTTCACCGTAAAATTACTGCTAGAAAAACTAAACTGTG
AAGTCATTGATTTAGGACTATTACCTGATAACGAAGCTGAATTTGAAAAAGCATTTATTGCCGCACAAAGCCAAGCCGAT
TTAGTGATTACCAGTGGCGGTGTTTCCGTTGGTGAAGCGGATTTCACTAAAGCAGTATTGGAAAAAGTAGGCAAAGTAAA
TTTCTGGAAAATTGCAATCAAACCCGGCAAACCATTCGCTTTTGGTAAATTAGAAAATGCTTGGTTCTGTGGTTTACCCG
GCAATCCTGTTTCCGCATTAGTAACATTCTATCAACTTGTACAGCCATTAATCGCTAAACTGCAAGGACAAAAACAATGG
AAAAATCCACCGCACTTTTCAGCCATTGCTACAATGAATTTGAAAAAAGCAGTGGGACGTTTAGACTTCCAACGGGGTTT
TTATTACATCAATGAACAAGGTCAAATTGAAGTGCAATCAGTAGGTTTCCAAGGTTCTCATCTCTTTAGTGCTTTTGTAA
AAAGCAATTGTTTTATCGTGTTAGAACAAGAACGTGGCAATGTCTCTGCGGGCGAAACAGTGACAATTGAGCCTTTCAAT
CATTTATTAGGATAA

Upstream 100 bases:

>100_bases
AAAGGATAATAGGATGGTAAAATGAGAAAAATTGCAAATTTGAATGAATTAGGCTTTAGATATAGAATAGCTGACAAATT
TTATCAAATAAGGATTTTCT

Downstream 100 bases:

>100_bases
AAAATGATCGAACTTAGTCACGAAGAAGAATTGCGTTATAACCGTCAAATTATTCTTAAAAGCGTAGATTTTGACGGGCA
AGAAAAACTTAAAGCAAGCA

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: MPT Mo-transferase [H]

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MLTLDQARSKMLEQLPFPTQTEYLNLQEAANRICAEDIISPINVPSFDNSAMDGYAVRLSDLQQSLTLSVAGKSFAGNPF
KEEWPSKSAVRIMTGAMIPEGTDAVIMQEQVTLNEDGTITFSELPKPNQNIRRIGEDVKKGDVVLEQGAPLTPVSLPLLA
SLGIAEVKCYRQLKVGVLSTGDELVEVGKPLQSGQIYDTNRFTVKLLLEKLNCEVIDLGLLPDNEAEFEKAFIAAQSQAD
LVITSGGVSVGEADFTKAVLEKVGKVNFWKIAIKPGKPFAFGKLENAWFCGLPGNPVSALVTFYQLVQPLIAKLQGQKQW
KNPPHFSAIATMNLKKAVGRLDFQRGFYYINEQGQIEVQSVGFQGSHLFSAFVKSNCFIVLEQERGNVSAGETVTIEPFN
HLLG

Sequences:

>Translated_404_residues
MLTLDQARSKMLEQLPFPTQTEYLNLQEAANRICAEDIISPINVPSFDNSAMDGYAVRLSDLQQSLTLSVAGKSFAGNPF
KEEWPSKSAVRIMTGAMIPEGTDAVIMQEQVTLNEDGTITFSELPKPNQNIRRIGEDVKKGDVVLEQGAPLTPVSLPLLA
SLGIAEVKCYRQLKVGVLSTGDELVEVGKPLQSGQIYDTNRFTVKLLLEKLNCEVIDLGLLPDNEAEFEKAFIAAQSQAD
LVITSGGVSVGEADFTKAVLEKVGKVNFWKIAIKPGKPFAFGKLENAWFCGLPGNPVSALVTFYQLVQPLIAKLQGQKQW
KNPPHFSAIATMNLKKAVGRLDFQRGFYYINEQGQIEVQSVGFQGSHLFSAFVKSNCFIVLEQERGNVSAGETVTIEPFN
HLLG
>Mature_404_residues
MLTLDQARSKMLEQLPFPTQTEYLNLQEAANRICAEDIISPINVPSFDNSAMDGYAVRLSDLQQSLTLSVAGKSFAGNPF
KEEWPSKSAVRIMTGAMIPEGTDAVIMQEQVTLNEDGTITFSELPKPNQNIRRIGEDVKKGDVVLEQGAPLTPVSLPLLA
SLGIAEVKCYRQLKVGVLSTGDELVEVGKPLQSGQIYDTNRFTVKLLLEKLNCEVIDLGLLPDNEAEFEKAFIAAQSQAD
LVITSGGVSVGEADFTKAVLEKVGKVNFWKIAIKPGKPFAFGKLENAWFCGLPGNPVSALVTFYQLVQPLIAKLQGQKQW
KNPPHFSAIATMNLKKAVGRLDFQRGFYYINEQGQIEVQSVGFQGSHLFSAFVKSNCFIVLEQERGNVSAGETVTIEPFN
HLLG

Specific function: Catalyzes the insertion of molybdate into adenylated molybdopterin with the concomitant release of AMP [H]

COG id: COG0303

COG function: function code H; Molybdopterin biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the moeA family [H]

Homologues:

Organism=Homo sapiens, GI10880983, Length=396, Percent_Identity=33.5858585858586, Blast_Score=185, Evalue=7e-47,
Organism=Homo sapiens, GI66932975, Length=396, Percent_Identity=33.5858585858586, Blast_Score=184, Evalue=9e-47,
Organism=Escherichia coli, GI1787049, Length=404, Percent_Identity=56.4356435643564, Blast_Score=452, Evalue=1e-128,
Organism=Caenorhabditis elegans, GI25147659, Length=334, Percent_Identity=32.3353293413174, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17562606, Length=328, Percent_Identity=30.4878048780488, Blast_Score=117, Evalue=9e-27,
Organism=Drosophila melanogaster, GI17136978, Length=375, Percent_Identity=29.8666666666667, Blast_Score=138, Evalue=8e-33,
Organism=Drosophila melanogaster, GI24638841, Length=375, Percent_Identity=29.8666666666667, Blast_Score=138, Evalue=8e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020817
- InterPro:   IPR008284
- InterPro:   IPR005111
- InterPro:   IPR005110
- InterPro:   IPR001453 [H]

Pfam domain/function: PF00994 MoCF_biosynth; PF03454 MoeA_C; PF03453 MoeA_N [H]

EC number: NA

Molecular weight: Translated: 44242; Mature: 44242

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS01079 MOCF_BIOSYNTHESIS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTLDQARSKMLEQLPFPTQTEYLNLQEAANRICAEDIISPINVPSFDNSAMDGYAVRLS
CCCHHHHHHHHHHHCCCCCCHHEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEHH
DLQQSLTLSVAGKSFAGNPFKEEWPSKSAVRIMTGAMIPEGTDAVIMQEQVTLNEDGTIT
HHHHHEEEEECCCCCCCCCCHHHCCCCCCEEEEECCCCCCCCCEEEEEEEEEECCCCCEE
FSELPKPNQNIRRIGEDVKKGDVVLEQGAPLTPVSLPLLASLGIAEVKCYRQLKVGVLST
ECCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHCCHHHHHHHHHHHHEEECC
GDELVEVGKPLQSGQIYDTNRFTVKLLLEKLNCEVIDLGLLPDNEAEFEKAFIAAQSQAD
CHHHHHHCCCCCCCEEEECCCHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHCCCCCC
LVITSGGVSVGEADFTKAVLEKVGKVNFWKIAIKPGKPFAFGKLENAWFCGLPGNPVSAL
EEEECCCCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCEEEECCCCCEEECCCCCHHHHH
VTFYQLVQPLIAKLQGQKQWKNPPHFSAIATMNLKKAVGRLDFQRGFYYINEQGQIEVQS
HHHHHHHHHHHHHHCCCHHCCCCCCCEEEEEHHHHHHHHHCCHHCCEEEECCCCCEEEEE
VGFQGSHLFSAFVKSNCFIVLEQERGNVSAGETVTIEPFNHLLG
ECCCCHHHHHHHHHCCCEEEEEECCCCCCCCCEEEECCHHHHCC
>Mature Secondary Structure
MLTLDQARSKMLEQLPFPTQTEYLNLQEAANRICAEDIISPINVPSFDNSAMDGYAVRLS
CCCHHHHHHHHHHHCCCCCCHHEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEHH
DLQQSLTLSVAGKSFAGNPFKEEWPSKSAVRIMTGAMIPEGTDAVIMQEQVTLNEDGTIT
HHHHHEEEEECCCCCCCCCCHHHCCCCCCEEEEECCCCCCCCCEEEEEEEEEECCCCCEE
FSELPKPNQNIRRIGEDVKKGDVVLEQGAPLTPVSLPLLASLGIAEVKCYRQLKVGVLST
ECCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHCCHHHHHHHHHHHHEEECC
GDELVEVGKPLQSGQIYDTNRFTVKLLLEKLNCEVIDLGLLPDNEAEFEKAFIAAQSQAD
CHHHHHHCCCCCCCEEEECCCHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHCCCCCC
LVITSGGVSVGEADFTKAVLEKVGKVNFWKIAIKPGKPFAFGKLENAWFCGLPGNPVSAL
EEEECCCCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCEEEECCCCCEEECCCCCHHHHH
VTFYQLVQPLIAKLQGQKQWKNPPHFSAIATMNLKKAVGRLDFQRGFYYINEQGQIEVQS
HHHHHHHHHHHHHHCCCHHCCCCCCCEEEEEHHHHHHHHHCCHHCCEEEECCCCCEEEEE
VGFQGSHLFSAFVKSNCFIVLEQERGNVSAGETVTIEPFNHLLG
ECCCCHHHHHHHHHCCCEEEEEECCCCCCCCCEEEECCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]