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The map label for this gene is metF [H]

Identifier: 148825987

GI number: 148825987

Start: 986568

End: 987446

Strand: Direct

Name: metF [H]

Synonym: CGSHiEE_04820

Alternate gene names: 148825987

Gene position: 986568-987446 (Clockwise)

Preceding gene: 148825982

Following gene: 148825991

Centisome position: 54.42

GC content: 36.52

Gene sequence:

>879_bases
ATGAGCTACGCGAAAGAAATTGATACATTAAATCAACATATTGCAGATTTTAATAAAAAAATTAATGTCTCCTTTGAATT
TTTTCCACCTAAAAACGAAAAAATGGAAACCCTTCTATGGGATTCAATTCATCGTTTAAAAGTATTAAAGCCTAAATTTG
TGTCAGTCACTTACGGCGCAAATTCGGGAGAACGTGACCGCACTCACGGCATTGTGAAAGCCATTAAACAAGAAACTGGC
TTAGAAGCCGCACCACATTTAACTGGAATTGATGCCACACCTGAAGAATTAAAACAAATTGCGAGAGATTATTGGGATAG
TGGTATTCGCCGTATTGTTGCGTTACGCGGTGACGAACCTAAAGGTTACGCGAAAAAACCATTTTATGCGTCAGATCTTG
TGGAATTACTCCGTTCTGTCGCTGATTTTGATATTTCTGTAGCCGCTTATCCTGAAGTTCATCCAGAAGCAAAATCCGCA
CAAGCAGACTTAATTAATTTAAAACGTAAAATTGATTCAGGTGCAAACCACGTCATTACACAATTTTTCTTTGATATTGA
AAACTACCTACGTTTTCGTGATCGTTGTGCATCAATTGGTATTGATACTGAAATCGTACCCGGTATTTTACCTGTTACTA
ATTTTAAACAACTCCAAAAAATGGCATCATTCACTAATGTGAAAATTCCAGCGTGGTTAGTTAAAGCCTATGATGGTTTG
GATGATGATCCAACTACACGTAATCTTGTAGCAGCAAGTGTTGCAATGGATATGGTAAAAATTTTATCTCGCGAAGGCGT
GAATGACTTCCACTTTTATACATTAAATCGTAGTGAATTAACTTATGCTATCTGTCATATGTTAGGTGTAAGACCTTAA

Upstream 100 bases:

>100_bases
ATTAATAATAATCATACTTTTAATGAATAAATTGAAATTGACTCATATTAAAACCTATTTTATTGTACAAATACTATTTT
AGAATCCTAAAGGAAGCCTT

Downstream 100 bases:

>100_bases
TTATCACAATAATATATTTCTTTTCAAACACAAAATAAGGGCGATATTTCGCCCTTATACAAAACTAAAAAAATTCAACC
GCACTTTATTATTTTATGAA

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MSYAKEIDTLNQHIADFNKKINVSFEFFPPKNEKMETLLWDSIHRLKVLKPKFVSVTYGANSGERDRTHGIVKAIKQETG
LEAAPHLTGIDATPEELKQIARDYWDSGIRRIVALRGDEPKGYAKKPFYASDLVELLRSVADFDISVAAYPEVHPEAKSA
QADLINLKRKIDSGANHVITQFFFDIENYLRFRDRCASIGIDTEIVPGILPVTNFKQLQKMASFTNVKIPAWLVKAYDGL
DDDPTTRNLVAASVAMDMVKILSREGVNDFHFYTLNRSELTYAICHMLGVRP

Sequences:

>Translated_292_residues
MSYAKEIDTLNQHIADFNKKINVSFEFFPPKNEKMETLLWDSIHRLKVLKPKFVSVTYGANSGERDRTHGIVKAIKQETG
LEAAPHLTGIDATPEELKQIARDYWDSGIRRIVALRGDEPKGYAKKPFYASDLVELLRSVADFDISVAAYPEVHPEAKSA
QADLINLKRKIDSGANHVITQFFFDIENYLRFRDRCASIGIDTEIVPGILPVTNFKQLQKMASFTNVKIPAWLVKAYDGL
DDDPTTRNLVAASVAMDMVKILSREGVNDFHFYTLNRSELTYAICHMLGVRP
>Mature_291_residues
SYAKEIDTLNQHIADFNKKINVSFEFFPPKNEKMETLLWDSIHRLKVLKPKFVSVTYGANSGERDRTHGIVKAIKQETGL
EAAPHLTGIDATPEELKQIARDYWDSGIRRIVALRGDEPKGYAKKPFYASDLVELLRSVADFDISVAAYPEVHPEAKSAQ
ADLINLKRKIDSGANHVITQFFFDIENYLRFRDRCASIGIDTEIVPGILPVTNFKQLQKMASFTNVKIPAWLVKAYDGLD
DDPTTRNLVAASVAMDMVKILSREGVNDFHFYTLNRSELTYAICHMLGVRP

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=283, Percent_Identity=33.2155477031802, Blast_Score=138, Evalue=5e-33,
Organism=Escherichia coli, GI1790377, Length=290, Percent_Identity=73.1034482758621, Blast_Score=457, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI25147436, Length=278, Percent_Identity=29.4964028776978, Blast_Score=126, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI25147441, Length=278, Percent_Identity=29.4964028776978, Blast_Score=126, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6325234, Length=294, Percent_Identity=31.9727891156463, Blast_Score=135, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6321313, Length=301, Percent_Identity=29.5681063122924, Blast_Score=128, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 33037; Mature: 32906

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYAKEIDTLNQHIADFNKKINVSFEFFPPKNEKMETLLWDSIHRLKVLKPKFVSVTYGA
CCHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEEECC
NSGERDRTHGIVKAIKQETGLEAAPHLTGIDATPEELKQIARDYWDSGIRRIVALRGDEP
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEHHCCCCCC
KGYAKKPFYASDLVELLRSVADFDISVAAYPEVHPEAKSAQADLINLKRKIDSGANHVIT
CCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCHHHHHH
QFFFDIENYLRFRDRCASIGIDTEIVPGILPVTNFKQLQKMASFTNVKIPAWLVKAYDGL
HHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCC
DDDPTTRNLVAASVAMDMVKILSREGVNDFHFYTLNRSELTYAICHMLGVRP
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SYAKEIDTLNQHIADFNKKINVSFEFFPPKNEKMETLLWDSIHRLKVLKPKFVSVTYGA
CHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEEECC
NSGERDRTHGIVKAIKQETGLEAAPHLTGIDATPEELKQIARDYWDSGIRRIVALRGDEP
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEHHCCCCCC
KGYAKKPFYASDLVELLRSVADFDISVAAYPEVHPEAKSAQADLINLKRKIDSGANHVIT
CCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCHHHHHH
QFFFDIENYLRFRDRCASIGIDTEIVPGILPVTNFKQLQKMASFTNVKIPAWLVKAYDGL
HHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCC
DDDPTTRNLVAASVAMDMVKILSREGVNDFHFYTLNRSELTYAICHMLGVRP
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]