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The map label for this gene is pyrF [H]

Identifier: 148825818

GI number: 148825818

Start: 792137

End: 792829

Strand: Direct

Name: pyrF [H]

Synonym: CGSHiEE_03855

Alternate gene names: 148825818

Gene position: 792137-792829 (Clockwise)

Preceding gene: 148825817

Following gene: 148825819

Centisome position: 43.69

GC content: 39.39

Gene sequence:

>693_bases
ATGACAAGTAAAATTATTGTGGCATTGGATTATGAGAAAGAAGCAGAGGCTCTTGCTTTAGTTGATCAAATTGATCCAAG
TTTGTGTCGATTAAAAGTGGGTAAAGAAATGTTTACTACACTTGGTATTAATTTTGTAAAACAATTACATCAACGTAATT
TTGATGTTTTTTTGGATCTGAAATATCACGATATTCCAAATACGGTAGCAAGAGCAGTTCGTTCTGCTGCTGATTTAGGC
GTATGGATGGTGGATCTACACGCAAGTGGTGGCTTGCGTATGATGGAAGAAGCGAAGAAAATTCTTGAACCCTATGGGAA
AGATGCACCATTGCTTATTGCTGTGACGGTATTAACCAGTATGGAAGACTTGGATTTATTACAAATTGGGATTAATGCAT
CGCCAATGGAACAAGTTTTACGCTTAGCTCATTTAACCCAACGGGCGGGTTTAGATGGTGTAGTCTGTTCCCCTCAAGAA
GTTGAAATTTTACGTAACGCTTGTGGAGAAGACTTTAAATTAGTGACCCCAGGGATTCGTCCAATAGGAACTGATTTTGG
CGATCAAAGACGTGTAATGACGCCTACTGCAGCAATTCGTGCAGGTTCAGATTATTTAGTTATTGGACGCCCAATAACTC
AAGCGGATAATCCTGCGGAGGTTCTCCGTTCTATTAATGTATCTATTGGGTAA

Upstream 100 bases:

>100_bases
ATAAGCTATTATGGAATTGCCCATCTTGCCGTCAATGGGAAAGTATTAAACCAGTTTCAAATCAAGAACATAATTAATTG
ATACTCAGTGAGGTAACACT

Downstream 100 bases:

>100_bases
TTATAAATGTCAGATTCTGTTTTAGTGTATTCAACAGATGTGGGTCGAATTAAAGAGGAAAAGGCTTCTGTAGTTCGCCC
AAAAGGAGACGGTGTTGTAC

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase [H]

Number of amino acids: Translated: 230; Mature: 229

Protein sequence:

>230_residues
MTSKIIVALDYEKEAEALALVDQIDPSLCRLKVGKEMFTTLGINFVKQLHQRNFDVFLDLKYHDIPNTVARAVRSAADLG
VWMVDLHASGGLRMMEEAKKILEPYGKDAPLLIAVTVLTSMEDLDLLQIGINASPMEQVLRLAHLTQRAGLDGVVCSPQE
VEILRNACGEDFKLVTPGIRPIGTDFGDQRRVMTPTAAIRAGSDYLVIGRPITQADNPAEVLRSINVSIG

Sequences:

>Translated_230_residues
MTSKIIVALDYEKEAEALALVDQIDPSLCRLKVGKEMFTTLGINFVKQLHQRNFDVFLDLKYHDIPNTVARAVRSAADLG
VWMVDLHASGGLRMMEEAKKILEPYGKDAPLLIAVTVLTSMEDLDLLQIGINASPMEQVLRLAHLTQRAGLDGVVCSPQE
VEILRNACGEDFKLVTPGIRPIGTDFGDQRRVMTPTAAIRAGSDYLVIGRPITQADNPAEVLRSINVSIG
>Mature_229_residues
TSKIIVALDYEKEAEALALVDQIDPSLCRLKVGKEMFTTLGINFVKQLHQRNFDVFLDLKYHDIPNTVARAVRSAADLGV
WMVDLHASGGLRMMEEAKKILEPYGKDAPLLIAVTVLTSMEDLDLLQIGINASPMEQVLRLAHLTQRAGLDGVVCSPQEV
EILRNACGEDFKLVTPGIRPIGTDFGDQRRVMTPTAAIRAGSDYLVIGRPITQADNPAEVLRSINVSIG

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP) [H]

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787537, Length=228, Percent_Identity=62.719298245614, Blast_Score=294, Evalue=4e-81,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00215 OMPdecase [H]

EC number: =4.1.1.23 [H]

Molecular weight: Translated: 25223; Mature: 25091

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00156 OMPDECASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSKIIVALDYEKEAEALALVDQIDPSLCRLKVGKEMFTTLGINFVKQLHQRNFDVFLDL
CCCEEEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
KYHDIPNTVARAVRSAADLGVWMVDLHASGGLRMMEEAKKILEPYGKDAPLLIAVTVLTS
EECCCCHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCEEHHHHHHC
MEDLDLLQIGINASPMEQVLRLAHLTQRAGLDGVVCSPQEVEILRNACGEDFKLVTPGIR
CCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHCCCCCCEEECCCCC
PIGTDFGDQRRVMTPTAAIRAGSDYLVIGRPITQADNPAEVLRSINVSIG
CCCCCCCCCCEEECCHHHHCCCCCEEEECCCCCCCCCHHHHHHHHCCCCC
>Mature Secondary Structure 
TSKIIVALDYEKEAEALALVDQIDPSLCRLKVGKEMFTTLGINFVKQLHQRNFDVFLDL
CCEEEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
KYHDIPNTVARAVRSAADLGVWMVDLHASGGLRMMEEAKKILEPYGKDAPLLIAVTVLTS
EECCCCHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCEEHHHHHHC
MEDLDLLQIGINASPMEQVLRLAHLTQRAGLDGVVCSPQEVEILRNACGEDFKLVTPGIR
CCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHCCCCCCEEECCCCC
PIGTDFGDQRRVMTPTAAIRAGSDYLVIGRPITQADNPAEVLRSINVSIG
CCCCCCCCCCEEECCHHHHCCCCCEEEECCCCCCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]