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The map label for this gene is lepB [H]

Identifier: 148825704

GI number: 148825704

Start: 651208

End: 652257

Strand: Direct

Name: lepB [H]

Synonym: CGSHiEE_03235

Alternate gene names: 148825704

Gene position: 651208-652257 (Clockwise)

Preceding gene: 148825703

Following gene: 148825705

Centisome position: 35.92

GC content: 38.86

Gene sequence:

>1050_bases
ATGTCAAATTTATTTTTTGTGATTTTATTGGCTGTCGGCTTTGGTGTGTGGAAAGTTTTAGATTATTTTCAGTTGCCAAA
TACTTTTAGTATTTTGTTACTAATTTTGACCGCACTTTCTGGCGTATTATGGTGTTATCATCGTTTTGTGGTGCTGCCAA
AACGTCATCGTCAAGTGGCACGTGCAGAACAACGTTCTGGTAAAACCTTAAGTGAGGAAGAAAAAGCCAAAATTGAACCG
ATTTCTGAGGCTTCAGAATTTTTGTCTTCACTTTTTCCTGTGCTTGCAGTGGTATTTTTGGTTCGTTCTTTTTTGTTTGA
ACCGTTTCAAATTCCCTCTGGCTCAATGGAGTCCACTTTACGCGTTGGCGATTTTTTAGTTGTGAATAAATATGCTTATG
GTGTGAAAGATCCGATTTTCCAAAACACCATTATTGAGGGCGAAAAACCACAACGTGGCGATGTGATTGTGTTTAAAGCA
CCACAACAAGCGTTAATTCGTACTGGTCTTGGGGCTACTCGAGCGGCTTTTGCAGAAAATTTAGCGTTAAGTTCAAAAGA
TAATATGTCTGGTGTGGATTATATTAAGCGTATTGTTGGAAAGGGCGGAGATCGCATCATTTTTGATGTGGAACAAAAAA
CATTAAAAATTGTATATGGCAAAGATGGTAAACCTTGTGAAGTTGATTGCGAAACCAAGGCGTTTGAATATACACAAAAT
CCAACAAATCCTGCTTTTCCGAATGAATTAGAATTGACTGAAAAAGGCGATGTAACACATAACGTGTTAATTGGTGAGTA
TCGTCGTTATTCAGACCTTGAATTTTTCCCACAAGAGGGAATGCAAACTGCAGAATGGCTTGTGCCAGAGGGGCAGTATT
TTGTGATGGGGGATCATCGCGATCACAGCGATGACAGTCGTTTTTGGGGCTTTGTGCCTGAAAAAAATATTGTGGGGAAA
GCCACTTATATTTGGATGAGCTTAGAAAAAGAAGCGAATGAATGGCCAACAGGTTTCCGTTTTGATCGCTTCTTTACAGC
AATAAAATAA

Upstream 100 bases:

>100_bases
AAGAAGGGAAAAAACGTATGAAGTCTTTAGGTAACGTAGAAGTGCCGCAAGAGGCGTTTTTAGCAATTTTACACGTAGGA
AAAGATAAATAAGGAAAATT

Downstream 100 bases:

>100_bases
GATGAATCATTTAGATCGACTTGAGCGTAAAATCGGCTATTGTTTTAATGATATTGCGCTCTTAAAGCAGGCACTTACGC
ACCGAAGTGCGGCAACTCAA

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: SPase I; Leader peptidase I [H]

Number of amino acids: Translated: 349; Mature: 348

Protein sequence:

>349_residues
MSNLFFVILLAVGFGVWKVLDYFQLPNTFSILLLILTALSGVLWCYHRFVVLPKRHRQVARAEQRSGKTLSEEEKAKIEP
ISEASEFLSSLFPVLAVVFLVRSFLFEPFQIPSGSMESTLRVGDFLVVNKYAYGVKDPIFQNTIIEGEKPQRGDVIVFKA
PQQALIRTGLGATRAAFAENLALSSKDNMSGVDYIKRIVGKGGDRIIFDVEQKTLKIVYGKDGKPCEVDCETKAFEYTQN
PTNPAFPNELELTEKGDVTHNVLIGEYRRYSDLEFFPQEGMQTAEWLVPEGQYFVMGDHRDHSDDSRFWGFVPEKNIVGK
ATYIWMSLEKEANEWPTGFRFDRFFTAIK

Sequences:

>Translated_349_residues
MSNLFFVILLAVGFGVWKVLDYFQLPNTFSILLLILTALSGVLWCYHRFVVLPKRHRQVARAEQRSGKTLSEEEKAKIEP
ISEASEFLSSLFPVLAVVFLVRSFLFEPFQIPSGSMESTLRVGDFLVVNKYAYGVKDPIFQNTIIEGEKPQRGDVIVFKA
PQQALIRTGLGATRAAFAENLALSSKDNMSGVDYIKRIVGKGGDRIIFDVEQKTLKIVYGKDGKPCEVDCETKAFEYTQN
PTNPAFPNELELTEKGDVTHNVLIGEYRRYSDLEFFPQEGMQTAEWLVPEGQYFVMGDHRDHSDDSRFWGFVPEKNIVGK
ATYIWMSLEKEANEWPTGFRFDRFFTAIK
>Mature_348_residues
SNLFFVILLAVGFGVWKVLDYFQLPNTFSILLLILTALSGVLWCYHRFVVLPKRHRQVARAEQRSGKTLSEEEKAKIEPI
SEASEFLSSLFPVLAVVFLVRSFLFEPFQIPSGSMESTLRVGDFLVVNKYAYGVKDPIFQNTIIEGEKPQRGDVIVFKAP
QQALIRTGLGATRAAFAENLALSSKDNMSGVDYIKRIVGKGGDRIIFDVEQKTLKIVYGKDGKPCEVDCETKAFEYTQNP
TNPAFPNELELTEKGDVTHNVLIGEYRRYSDLEFFPQEGMQTAEWLVPEGQYFVMGDHRDHSDDSRFWGFVPEKNIVGKA
TYIWMSLEKEANEWPTGFRFDRFFTAIK

Specific function: Unknown

COG id: COG0681

COG function: function code U; Signal peptidase I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S26 family [H]

Homologues:

Organism=Escherichia coli, GI1788921, Length=346, Percent_Identity=40.7514450867052, Blast_Score=256, Evalue=1e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000223
- InterPro:   IPR019758
- InterPro:   IPR019757
- InterPro:   IPR019756
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR019533
- InterPro:   IPR019766 [H]

Pfam domain/function: PF00717 Peptidase_S24; PF10502 Peptidase_S26 [H]

EC number: =3.4.21.89 [H]

Molecular weight: Translated: 39748; Mature: 39617

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS00501 SPASE_I_1 ; PS00760 SPASE_I_2 ; PS00761 SPASE_I_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNLFFVILLAVGFGVWKVLDYFQLPNTFSILLLILTALSGVLWCYHRFVVLPKRHRQVA
CCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
RAEQRSGKTLSEEEKAKIEPISEASEFLSSLFPVLAVVFLVRSFLFEPFQIPSGSMESTL
HHHHHCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
RVGDFLVVNKYAYGVKDPIFQNTIIEGEKPQRGDVIVFKAPQQALIRTGLGATRAAFAEN
HHCCEEEEECCCCCCCCCHHHCCCCCCCCCCCCCEEEEECCHHHHHHHCCCHHHHHHHHH
LALSSKDNMSGVDYIKRIVGKGGDRIIFDVEQKTLKIVYGKDGKPCEVDCETKAFEYTQN
HCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCEEEEEECCCCCCCEEECCCCCEECCCC
PTNPAFPNELELTEKGDVTHNVLIGEYRRYSDLEFFPQEGMQTAEWLVPEGQYFVMGDHR
CCCCCCCCCCCCCCCCCCCHHEEEHHHHCCCCCCCCCCCCCCHHHHCCCCCCEEEEECCC
DHSDDSRFWGFVPEKNIVGKATYIWMSLEKEANEWPTGFRFDRFFTAIK
CCCCCCCEECCCCCCCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHCC
>Mature Secondary Structure 
SNLFFVILLAVGFGVWKVLDYFQLPNTFSILLLILTALSGVLWCYHRFVVLPKRHRQVA
CCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
RAEQRSGKTLSEEEKAKIEPISEASEFLSSLFPVLAVVFLVRSFLFEPFQIPSGSMESTL
HHHHHCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
RVGDFLVVNKYAYGVKDPIFQNTIIEGEKPQRGDVIVFKAPQQALIRTGLGATRAAFAEN
HHCCEEEEECCCCCCCCCHHHCCCCCCCCCCCCCEEEEECCHHHHHHHCCCHHHHHHHHH
LALSSKDNMSGVDYIKRIVGKGGDRIIFDVEQKTLKIVYGKDGKPCEVDCETKAFEYTQN
HCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCEEEEEECCCCCCCEEECCCCCEECCCC
PTNPAFPNELELTEKGDVTHNVLIGEYRRYSDLEFFPQEGMQTAEWLVPEGQYFVMGDHR
CCCCCCCCCCCCCCCCCCCHHEEEHHHHCCCCCCCCCCCCCCHHHHCCCCCCEEEEECCC
DHSDDSRFWGFVPEKNIVGKATYIWMSLEKEANEWPTGFRFDRFFTAIK
CCCCCCCEECCCCCCCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]