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The map label for this gene is grcA

Identifier: 148825702

GI number: 148825702

Start: 648851

End: 649234

Strand: Direct

Name: grcA

Synonym: CGSHiEE_03225

Alternate gene names: 148825702

Gene position: 648851-649234 (Clockwise)

Preceding gene: 148825700

Following gene: 148825703

Centisome position: 35.79

GC content: 38.28

Gene sequence:

>384_bases
ATGATTAAAGGTATTCAAATTACTCAAGCGGCAAATGACAATTTGCTTAATTCATTCTGGTTATTAGACAGCGAAAAAAA
TGAAGTGCGTTGCTTATGTGCAAAAGGCGAATTCGCTGAAGACCAAGTTGTTGCAGTGAGCGAATTAGGTCAAATCGAAT
ACCGTGAATTACCAGTAAACGTAGCACCAACTGTAAAAGTTGAAGGTGGCCAACATTTAAACGTGAACGTATTACGTCGT
GAAACTTTAGAAGATGCGGTAAATAACCCAGATAAATATCCACAATTAACTATCCGTGTTTCTGGTTACGCAGTACGTTT
CAACTCTTTAACACCAGAACAACAACGCGACGTTATCACTCGTACTTTCACTGAAAGCCTATAA

Upstream 100 bases:

>100_bases
GCTCTTTATCAAATAAATCTAAAATAATTTGAGATTTTAAAATGATTTGATAAAATTTGTACAGTTTTATTATTATTTTC
GTGCCAATTAGGAGGCTATT

Downstream 100 bases:

>100_bases
TTTCAGTTTGAATGAAAAAAAAGAAACCCCGAAGTTATTCGGGGTTTTGTTTTATCTAAAAGTTTGAAAATTTGACCGCA
CTTTTCCTTTTCATCTCTAG

Product: autonomous glycyl radical cofactor GrcA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 127; Mature: 127

Protein sequence:

>127_residues
MIKGIQITQAANDNLLNSFWLLDSEKNEVRCLCAKGEFAEDQVVAVSELGQIEYRELPVNVAPTVKVEGGQHLNVNVLRR
ETLEDAVNNPDKYPQLTIRVSGYAVRFNSLTPEQQRDVITRTFTESL

Sequences:

>Translated_127_residues
MIKGIQITQAANDNLLNSFWLLDSEKNEVRCLCAKGEFAEDQVVAVSELGQIEYRELPVNVAPTVKVEGGQHLNVNVLRR
ETLEDAVNNPDKYPQLTIRVSGYAVRFNSLTPEQQRDVITRTFTESL
>Mature_127_residues
MIKGIQITQAANDNLLNSFWLLDSEKNEVRCLCAKGEFAEDQVVAVSELGQIEYRELPVNVAPTVKVEGGQHLNVNVLRR
ETLEDAVNNPDKYPQLTIRVSGYAVRFNSLTPEQQRDVITRTFTESL

Specific function: Acts as a radical domain for damaged PFL and possibly other radical proteins

COG id: COG3445

COG function: function code R; Acid-induced glycyl radical enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glycine radical domain

Homologues:

Organism=Escherichia coli, GI1788933, Length=127, Percent_Identity=82.6771653543307, Blast_Score=221, Evalue=9e-60,
Organism=Escherichia coli, GI1787131, Length=61, Percent_Identity=80.327868852459, Blast_Score=108, Evalue=1e-25,
Organism=Escherichia coli, GI48994926, Length=61, Percent_Identity=75.4098360655738, Blast_Score=101, Evalue=2e-23,
Organism=Escherichia coli, GI1787044, Length=57, Percent_Identity=45.6140350877193, Blast_Score=63, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GRCA_HAEI8 (Q4QPM7)

Other databases:

- EMBL:   CP000057
- RefSeq:   YP_247680.1
- HSSP:   P09373
- ProteinModelPortal:   Q4QPM7
- SMR:   Q4QPM7
- STRING:   Q4QPM7
- GeneID:   3429422
- GenomeReviews:   CP000057_GR
- KEGG:   hit:NTHI0022
- eggNOG:   COG3445
- HOGENOM:   HBG297207
- OMA:   GQFEYRE
- PhylomeDB:   Q4QPM7
- ProtClustDB:   PRK11127
- BioCyc:   HINF281310:NTHI0022-MONOMER
- HAMAP:   MF_00806
- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR011140
- PIRSF:   PIRSF000378

Pfam domain/function: PF01228 Gly_radical

EC number: NA

Molecular weight: Translated: 14319; Mature: 14319

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00850 GLY_RADICAL_1; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKGIQITQAANDNLLNSFWLLDSEKNEVRCLCAKGEFAEDQVVAVSELGQIEYRELPVN
CCCCEEEEECCCCCCCEEEEEEECCCCCEEEEEECCCCCCCEEEEEHHCCCEEEEECCCC
VAPTVKVEGGQHLNVNVLRRETLEDAVNNPDKYPQLTIRVSGYAVRFNSLTPEQQRDVIT
CCCEEEECCCCEEEEEEEHHHHHHHHHCCCCCCCEEEEEECCEEEEECCCCCHHHHHHHH
RTFTESL
HHHHHCC
>Mature Secondary Structure
MIKGIQITQAANDNLLNSFWLLDSEKNEVRCLCAKGEFAEDQVVAVSELGQIEYRELPVN
CCCCEEEEECCCCCCCEEEEEEECCCCCEEEEEECCCCCCCEEEEEHHCCCEEEEECCCC
VAPTVKVEGGQHLNVNVLRRETLEDAVNNPDKYPQLTIRVSGYAVRFNSLTPEQQRDVIT
CCCEEEECCCCEEEEEEEHHHHHHHHHCCCCCCCEEEEEECCEEEEECCCCCHHHHHHHH
RTFTESL
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA