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The map label for this gene is miaB

Identifier: 148825700

GI number: 148825700

Start: 646440

End: 647864

Strand: Direct

Name: miaB

Synonym: CGSHiEE_03215

Alternate gene names: 148825700

Gene position: 646440-647864 (Clockwise)

Preceding gene: 148825699

Following gene: 148825702

Centisome position: 35.66

GC content: 41.19

Gene sequence:

>1425_bases
ATGACTCAAAAATTACATATTAAAACTTGGGGCTGTCAGATGAATGAATATGATTCATCTAAAATGGCCGATCTTTTATT
AAGCACTCACGGATTAGAACTCACGGAAGCACCTGAAGAAGCAGACGTATTATTATTGAATACCTGTTCTATTCGTGAAA
AAGCACAAGAAAAAGTGTTTCATCAACTTGGGCGTTGGAAAGAATTAAAGAAAAATAATCCAAATTTAGTGATTGGCGTG
GGCGGCTGTGTGGCTTCTCAAGAAGGGGAGCATATTCGTCATCGCGCGCCTTATGTGGATATTATTTTTGGCCCACAAAC
TTTGCATCGTCTGCCTGAAATGATTAATCAAATTCGTGGTGGTAAAAGTTCTGTGGTTGATGTGAGTTTCCCTGAAATTG
AGAAATTTGACCGCTTACCTGAACCTCGCGCAGAAGGCCCAACTGCATTTGTTTCCATTATGGAAGGCTGTAATAAATAC
TGTACTTTCTGTGTTGTGCCTTATACTCGTGGGGAAGAAGTTAGTCGTCCTGTAGATGATGTGTTATTTGAAATTGCGCA
ACTTGCGGAACAAGGTGTGCGAGAGGTTAATTTGCTTGGTCAAAACGTGAATGCCTATCGTGGGCCAACGCACGATGGTC
AAATTTGTAGCTTTGCTGAACTGCTTCGTCTTGTCGCATCAATTGATGGTATTGACCGTTTACGTTTCACAACCAGTCAT
CCAATAGAATTTACAAATGATATCATTGATGTGTATCGTGACACGCCTGAATTAGTGAGTTTCTTGCATCTTCCAGTGCA
AGCAGGATCTGACCGTGTATTAACGATGATGAAACGTGGTCATACCGCATTAGAATATAAATCTATTATTCGTAAACTTC
GTGCAGTGCGTCCAGATATTCAAATCAGCTCTGACTTTATTGTTGGTTTCCCAGGGGAAACTGCGGAAGATTTTGAGCAG
ACTATGAATTTGATTGCACAAGTGAACTTTGATATGAGCTTCAGTTTTGTGTATTCTGCCCGACCAGGTACGCCTGCTGC
AGATATGCCAGATGATGTGACGGAAGATGAAAAGAAACAGCGTCTTTATGTGCTACAAGAGCGTATTAATCAACAAGCCG
CACAATTTAGTCGTCGTATGCTTGGCACAGAGCAGCGCGTGTTAGTTGAAGGGCCATCGAAGAAGGATATTATGGAATTA
ACAGGACGCACTGAAACAAATCGTATTGTTAATTTCCAAGGTTCTCCAGAAATGATCGGTAAGTTTGTGGATGTAAAAAT
TACTGATGTTTATACTAATTCGTTACGTGGCGAAGTGGTGCGTACTGAAGATGAAATGGGATTACGTATTGCACAATCGC
CACAAGAAGTGATGAATCGCACACGTAAAGAAGATGAATTAGGCGTAGGGCGTTATCACGGCTAA

Upstream 100 bases:

>100_bases
TTTCACCGAATATTCACTAGCCAGAATTATCGTAAGTCTGTAGAATAGCCGACCGCTTTATTCGACTAATTATTTATAAC
ACCTGACGATATTGTAGAAA

Downstream 100 bases:

>100_bases
TTAAAATGCTGAAATATTTAACCGCACTTTGAGAGATTAAAGTGCGGTTATTTTTTAGAGATATATGGTTTAGATTTGCC
AATCAATTGGTTTCATTCCA

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MTQKLHIKTWGCQMNEYDSSKMADLLLSTHGLELTEAPEEADVLLLNTCSIREKAQEKVFHQLGRWKELKKNNPNLVIGV
GGCVASQEGEHIRHRAPYVDIIFGPQTLHRLPEMINQIRGGKSSVVDVSFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
CTFCVVPYTRGEEVSRPVDDVLFEIAQLAEQGVREVNLLGQNVNAYRGPTHDGQICSFAELLRLVASIDGIDRLRFTTSH
PIEFTNDIIDVYRDTPELVSFLHLPVQAGSDRVLTMMKRGHTALEYKSIIRKLRAVRPDIQISSDFIVGFPGETAEDFEQ
TMNLIAQVNFDMSFSFVYSARPGTPAADMPDDVTEDEKKQRLYVLQERINQQAAQFSRRMLGTEQRVLVEGPSKKDIMEL
TGRTETNRIVNFQGSPEMIGKFVDVKITDVYTNSLRGEVVRTEDEMGLRIAQSPQEVMNRTRKEDELGVGRYHG

Sequences:

>Translated_474_residues
MTQKLHIKTWGCQMNEYDSSKMADLLLSTHGLELTEAPEEADVLLLNTCSIREKAQEKVFHQLGRWKELKKNNPNLVIGV
GGCVASQEGEHIRHRAPYVDIIFGPQTLHRLPEMINQIRGGKSSVVDVSFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
CTFCVVPYTRGEEVSRPVDDVLFEIAQLAEQGVREVNLLGQNVNAYRGPTHDGQICSFAELLRLVASIDGIDRLRFTTSH
PIEFTNDIIDVYRDTPELVSFLHLPVQAGSDRVLTMMKRGHTALEYKSIIRKLRAVRPDIQISSDFIVGFPGETAEDFEQ
TMNLIAQVNFDMSFSFVYSARPGTPAADMPDDVTEDEKKQRLYVLQERINQQAAQFSRRMLGTEQRVLVEGPSKKDIMEL
TGRTETNRIVNFQGSPEMIGKFVDVKITDVYTNSLRGEVVRTEDEMGLRIAQSPQEVMNRTRKEDELGVGRYHG
>Mature_473_residues
TQKLHIKTWGCQMNEYDSSKMADLLLSTHGLELTEAPEEADVLLLNTCSIREKAQEKVFHQLGRWKELKKNNPNLVIGVG
GCVASQEGEHIRHRAPYVDIIFGPQTLHRLPEMINQIRGGKSSVVDVSFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYC
TFCVVPYTRGEEVSRPVDDVLFEIAQLAEQGVREVNLLGQNVNAYRGPTHDGQICSFAELLRLVASIDGIDRLRFTTSHP
IEFTNDIIDVYRDTPELVSFLHLPVQAGSDRVLTMMKRGHTALEYKSIIRKLRAVRPDIQISSDFIVGFPGETAEDFEQT
MNLIAQVNFDMSFSFVYSARPGTPAADMPDDVTEDEKKQRLYVLQERINQQAAQFSRRMLGTEQRVLVEGPSKKDIMELT
GRTETNRIVNFQGSPEMIGKFVDVKITDVYTNSLRGEVVRTEDEMGLRIAQSPQEVMNRTRKEDELGVGRYHG

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=480, Percent_Identity=37.2916666666667, Blast_Score=291, Evalue=1e-78,
Organism=Homo sapiens, GI28872782, Length=480, Percent_Identity=37.2916666666667, Blast_Score=290, Evalue=2e-78,
Organism=Homo sapiens, GI93277076, Length=450, Percent_Identity=29.7777777777778, Blast_Score=171, Evalue=1e-42,
Organism=Escherichia coli, GI1786882, Length=473, Percent_Identity=82.2410147991543, Blast_Score=829, Evalue=0.0,
Organism=Escherichia coli, GI1787057, Length=433, Percent_Identity=28.6374133949192, Blast_Score=167, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17553146, Length=464, Percent_Identity=34.2672413793103, Blast_Score=261, Evalue=5e-70,
Organism=Caenorhabditis elegans, GI71996771, Length=340, Percent_Identity=27.6470588235294, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI21356207, Length=433, Percent_Identity=37.1824480369515, Blast_Score=286, Evalue=3e-77,
Organism=Drosophila melanogaster, GI19922432, Length=491, Percent_Identity=30.142566191446, Blast_Score=166, Evalue=3e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_HAEI8 (Q4QPM5)

Other databases:

- EMBL:   CP000057
- RefSeq:   YP_247682.1
- ProteinModelPortal:   Q4QPM5
- SMR:   Q4QPM5
- STRING:   Q4QPM5
- GeneID:   3429424
- GenomeReviews:   CP000057_GR
- KEGG:   hit:NTHI0026
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   ANLHDDT
- PhylomeDB:   Q4QPM5
- ProtClustDB:   PRK14325
- BioCyc:   HINF281310:NTHI0026-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 53626; Mature: 53495

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQKLHIKTWGCQMNEYDSSKMADLLLSTHGLELTEAPEEADVLLLNTCSIREKAQEKVF
CCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCHHHHHHHHHHH
HQLGRWKELKKNNPNLVIGVGGCVASQEGEHIRHRAPYVDIIFGPQTLHRLPEMINQIRG
HHHHHHHHHHCCCCCEEEECCCEECCCCCCHHHHCCCEEEEEECHHHHHHHHHHHHHHCC
GKSSVVDVSFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYCTFCVVPYTRGEEVSRPVDD
CCCCEEECCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHEEEEEEECCCCCHHCCCHHH
VLFEIAQLAEQGVREVNLLGQNVNAYRGPTHDGQICSFAELLRLVASIDGIDRLRFTTSH
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHEEECCCC
PIEFTNDIIDVYRDTPELVSFLHLPVQAGSDRVLTMMKRGHTALEYKSIIRKLRAVRPDI
CCHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
QISSDFIVGFPGETAEDFEQTMNLIAQVNFDMSFSFVYSARPGTPAADMPDDVTEDEKKQ
EECCCEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHH
RLYVLQERINQQAAQFSRRMLGTEQRVLVEGPSKKDIMELTGRTETNRIVNFQGSPEMIG
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHCCCCCCCEEEEECCCHHHHH
KFVDVKITDVYTNSLRGEVVRTEDEMGLRIAQSPQEVMNRTRKEDELGVGRYHG
HHHEEEEEEEECCCCCCCEEECCHHCCCEECCCHHHHHHHHCCHHHCCCCCCCC
>Mature Secondary Structure 
TQKLHIKTWGCQMNEYDSSKMADLLLSTHGLELTEAPEEADVLLLNTCSIREKAQEKVF
CCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCHHHHHHHHHHH
HQLGRWKELKKNNPNLVIGVGGCVASQEGEHIRHRAPYVDIIFGPQTLHRLPEMINQIRG
HHHHHHHHHHCCCCCEEEECCCEECCCCCCHHHHCCCEEEEEECHHHHHHHHHHHHHHCC
GKSSVVDVSFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYCTFCVVPYTRGEEVSRPVDD
CCCCEEECCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHEEEEEEECCCCCHHCCCHHH
VLFEIAQLAEQGVREVNLLGQNVNAYRGPTHDGQICSFAELLRLVASIDGIDRLRFTTSH
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHEEECCCC
PIEFTNDIIDVYRDTPELVSFLHLPVQAGSDRVLTMMKRGHTALEYKSIIRKLRAVRPDI
CCHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
QISSDFIVGFPGETAEDFEQTMNLIAQVNFDMSFSFVYSARPGTPAADMPDDVTEDEKKQ
EECCCEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHH
RLYVLQERINQQAAQFSRRMLGTEQRVLVEGPSKKDIMELTGRTETNRIVNFQGSPEMIG
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHCCCCCCCEEEEECCCHHHHH
KFVDVKITDVYTNSLRGEVVRTEDEMGLRIAQSPQEVMNRTRKEDELGVGRYHG
HHHEEEEEEEECCCCCCCEEECCHHCCCEECCCHHHHHHHHCCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA