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The map label for this gene is sdaA [H]

Identifier: 148825434

GI number: 148825434

Start: 300396

End: 301763

Strand: Direct

Name: sdaA [H]

Synonym: CGSHiEE_01620

Alternate gene names: 148825434

Gene position: 300396-301763 (Clockwise)

Preceding gene: 148825433

Following gene: 148825437

Centisome position: 16.57

GC content: 40.94

Gene sequence:

>1368_bases
ATGATTAGCGTATTTGATATGTTTAAGGTGGGAATTGGGCCATCCAGTTCCCACACGGTCGGTCCGATGAAAGCGGGTAA
ACAATTTATAGACGACTTAATTAAACGAAATCAATTTGAACAAACTACTGAAATTCACGTAGATGTGTACGGTTCTCTTT
CAATGACAGGACGAGGACACAGTACAGATATTGCCATTATTATGGGGTTAGCGGGTTATTTACCACATAATGTGGATATT
GATATGATTTCAGGTTTCATCGAGAAAGTTAAACAAACTGCACTTTTACCCATTAATGTCGGTCAAAAGATAGTAAAATT
TGATTTTGAAAATAATCTCATTTTCCACCGCACTTTTTTAAAATTGCACGAAAATGGCATGACAATTACCGCTCTTGATG
AAAATCGCACAGAGCTTTATCGCCAAACCTATTATTCGATTGGGGGTGGTTTTATTGTCGATGAAGCCCATTTTGGCAAA
GAAGAAAAAAACACAGTCCAAGTGCCTTATCCTTACAAAAATGCCGAAGATATTTTGAAGCATTGCAGTGATAACGGCTT
AATGCTTTCCACTGTGATGTTGGAAAATGAAATCGCATTGAATGGCAAAGAGGCTGTCAGTGCTCATATAGAGAATGTTT
GGAAAACGATGCAGGCTTGTATTGAACACGGCATTCACACAGAGGGTATTTTACCCGGCCCATTAAAAGTACCACGTCGT
GCGGCTTCACTTTATCGTATGTTGCAAGCTAATACGAATTTATCTAACGATCCAATGCGTGTGATCGACTGGGTCAATAT
GTTTGCGCTTGCTGTCAATGAAGAAAATGCAGCGGGTGGACGTGTAGTCACAGCTCCAACTAATGGTGCGTGTGGGATTA
TTCCAGCTGTATTAGCTTACTACGAAAAATTTATCTCGCCATTAACATCTGAAATCATTGAACGCTATTTATTAGCAGCG
GGTATGATTGGTTCACTTTATAAAATGAACGCTTCGATTTCTGGCGCAGAAGTGGGTTGTCAAGGTGAAGTCGGTGTAGC
TTGTTCAATGGCGGCTGCAGGACTTGCTGAAATTCTGGGTGGCAATCCACTACAAGTGTGCATCGCAGCTGAAATTGCAA
TGGAGCATAATTTAGGATTAACTTGCGACCCTGTCGGCGGTCAAGTTCAAGTACCTTGTATCGAACGCAATGCGATTGCT
TCAGTTAAAGCAATCAATGCATCACGTATGGCTTTACGCCGTACCACTAATCCAAGAGTAAGCTTGGATAAAGTGATTGA
AACAATGTACGAAACAGGCAAAGATATGAACGCTAAGTATCGCGAAACATCGCAAGGCGGACTGGCAGTGAAGATTGTTT
GTAACTAA

Upstream 100 bases:

>100_bases
CAGTAATGTATTTGTAACAATTATGGGGCTAATTGCTATTTCTGCAGTTGTTTATGGATTATTATAATCGCCTTTAATTT
TAACGAATTTTGAGAAATTT

Downstream 100 bases:

>100_bases
TAAAAAGTAAAATAATTAAGGGGCTGATCTGCATGAATTAGTTGGACTACATAACCCACCTATAAGGTGCAGATCATTAT
TTAATCATTTAAATATCTTG

Product: L-serine deaminase

Products: NA

Alternate protein names: SDH; L-serine deaminase; L-SD [H]

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MISVFDMFKVGIGPSSSHTVGPMKAGKQFIDDLIKRNQFEQTTEIHVDVYGSLSMTGRGHSTDIAIIMGLAGYLPHNVDI
DMISGFIEKVKQTALLPINVGQKIVKFDFENNLIFHRTFLKLHENGMTITALDENRTELYRQTYYSIGGGFIVDEAHFGK
EEKNTVQVPYPYKNAEDILKHCSDNGLMLSTVMLENEIALNGKEAVSAHIENVWKTMQACIEHGIHTEGILPGPLKVPRR
AASLYRMLQANTNLSNDPMRVIDWVNMFALAVNEENAAGGRVVTAPTNGACGIIPAVLAYYEKFISPLTSEIIERYLLAA
GMIGSLYKMNASISGAEVGCQGEVGVACSMAAAGLAEILGGNPLQVCIAAEIAMEHNLGLTCDPVGGQVQVPCIERNAIA
SVKAINASRMALRRTTNPRVSLDKVIETMYETGKDMNAKYRETSQGGLAVKIVCN

Sequences:

>Translated_455_residues
MISVFDMFKVGIGPSSSHTVGPMKAGKQFIDDLIKRNQFEQTTEIHVDVYGSLSMTGRGHSTDIAIIMGLAGYLPHNVDI
DMISGFIEKVKQTALLPINVGQKIVKFDFENNLIFHRTFLKLHENGMTITALDENRTELYRQTYYSIGGGFIVDEAHFGK
EEKNTVQVPYPYKNAEDILKHCSDNGLMLSTVMLENEIALNGKEAVSAHIENVWKTMQACIEHGIHTEGILPGPLKVPRR
AASLYRMLQANTNLSNDPMRVIDWVNMFALAVNEENAAGGRVVTAPTNGACGIIPAVLAYYEKFISPLTSEIIERYLLAA
GMIGSLYKMNASISGAEVGCQGEVGVACSMAAAGLAEILGGNPLQVCIAAEIAMEHNLGLTCDPVGGQVQVPCIERNAIA
SVKAINASRMALRRTTNPRVSLDKVIETMYETGKDMNAKYRETSQGGLAVKIVCN
>Mature_455_residues
MISVFDMFKVGIGPSSSHTVGPMKAGKQFIDDLIKRNQFEQTTEIHVDVYGSLSMTGRGHSTDIAIIMGLAGYLPHNVDI
DMISGFIEKVKQTALLPINVGQKIVKFDFENNLIFHRTFLKLHENGMTITALDENRTELYRQTYYSIGGGFIVDEAHFGK
EEKNTVQVPYPYKNAEDILKHCSDNGLMLSTVMLENEIALNGKEAVSAHIENVWKTMQACIEHGIHTEGILPGPLKVPRR
AASLYRMLQANTNLSNDPMRVIDWVNMFALAVNEENAAGGRVVTAPTNGACGIIPAVLAYYEKFISPLTSEIIERYLLAA
GMIGSLYKMNASISGAEVGCQGEVGVACSMAAAGLAEILGGNPLQVCIAAEIAMEHNLGLTCDPVGGQVQVPCIERNAIA
SVKAINASRMALRRTTNPRVSLDKVIETMYETGKDMNAKYRETSQGGLAVKIVCN

Specific function: Deaminates Also Threonine, Particularly When It Is Present In High Concentration. [C]

COG id: COG1760

COG function: function code E; L-serine deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-sulfur dependent L-serine dehydratase family [H]

Homologues:

Organism=Escherichia coli, GI1788116, Length=455, Percent_Identity=69.010989010989, Blast_Score=664, Evalue=0.0,
Organism=Escherichia coli, GI1789161, Length=453, Percent_Identity=70.4194260485651, Blast_Score=662, Evalue=0.0,
Organism=Escherichia coli, GI48994925, Length=454, Percent_Identity=65.8590308370044, Blast_Score=620, Evalue=1e-179,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004644
- InterPro:   IPR005130
- InterPro:   IPR005131 [H]

Pfam domain/function: PF03313 SDH_alpha; PF03315 SDH_beta [H]

EC number: =4.3.1.17 [H]

Molecular weight: Translated: 49547; Mature: 49547

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISVFDMFKVGIGPSSSHTVGPMKAGKQFIDDLIKRNQFEQTTEIHVDVYGSLSMTGRGH
CCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEEEECCCCC
STDIAIIMGLAGYLPHNVDIDMISGFIEKVKQTALLPINVGQKIVKFDFENNLIFHRTFL
CCCHHHEEHHHHHCCCCCCHHHHHHHHHHHHHHEEEEECCCCEEEEEECCCCEEEEEHEE
KLHENGMTITALDENRTELYRQTYYSIGGGFIVDEAHFGKEEKNTVQVPYPYKNAEDILK
HHHCCCCEEEEECCCHHHHHHHHHHHCCCCEEEEHHHCCCCCCCEEECCCCCCCHHHHHH
HCSDNGLMLSTVMLENEIALNGKEAVSAHIENVWKTMQACIEHGIHTEGILPGPLKVPRR
HCCCCCEEEEEEEECCCEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHH
AASLYRMLQANTNLSNDPMRVIDWVNMFALAVNEENAAGGRVVTAPTNGACGIIPAVLAY
HHHHHHHHHHCCCCCCCHHHHHHHHHHHEEEECCCCCCCCEEEECCCCCCCHHHHHHHHH
YEKFISPLTSEIIERYLLAAGMIGSLYKMNASISGAEVGCQGEVGVACSMAAAGLAEILG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
GNPLQVCIAAEIAMEHNLGLTCDPVGGQVQVPCIERNAIASVKAINASRMALRRTTNPRV
CCCEEEEEEEHHHHHCCCCEEECCCCCEEEEEEECCCHHHHHHHCCHHHHHHHCCCCCCC
SLDKVIETMYETGKDMNAKYRETSQGGLAVKIVCN
CHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEC
>Mature Secondary Structure
MISVFDMFKVGIGPSSSHTVGPMKAGKQFIDDLIKRNQFEQTTEIHVDVYGSLSMTGRGH
CCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEEEECCCCC
STDIAIIMGLAGYLPHNVDIDMISGFIEKVKQTALLPINVGQKIVKFDFENNLIFHRTFL
CCCHHHEEHHHHHCCCCCCHHHHHHHHHHHHHHEEEEECCCCEEEEEECCCCEEEEEHEE
KLHENGMTITALDENRTELYRQTYYSIGGGFIVDEAHFGKEEKNTVQVPYPYKNAEDILK
HHHCCCCEEEEECCCHHHHHHHHHHHCCCCEEEEHHHCCCCCCCEEECCCCCCCHHHHHH
HCSDNGLMLSTVMLENEIALNGKEAVSAHIENVWKTMQACIEHGIHTEGILPGPLKVPRR
HCCCCCEEEEEEEECCCEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHH
AASLYRMLQANTNLSNDPMRVIDWVNMFALAVNEENAAGGRVVTAPTNGACGIIPAVLAY
HHHHHHHHHHCCCCCCCHHHHHHHHHHHEEEECCCCCCCCEEEECCCCCCCHHHHHHHHH
YEKFISPLTSEIIERYLLAAGMIGSLYKMNASISGAEVGCQGEVGVACSMAAAGLAEILG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
GNPLQVCIAAEIAMEHNLGLTCDPVGGQVQVPCIERNAIASVKAINASRMALRRTTNPRV
CCCEEEEEEEHHHHHCCCCEEECCCCCEEEEEEECCCHHHHHHHCCHHHHHHHCCCCCCC
SLDKVIETMYETGKDMNAKYRETSQGGLAVKIVCN
CHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]