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The map label for this gene is menF [H]

Identifier: 148825437

GI number: 148825437

Start: 304612

End: 305904

Strand: Direct

Name: menF [H]

Synonym: CGSHiEE_01635

Alternate gene names: 148825437

Gene position: 304612-305904 (Clockwise)

Preceding gene: 148825434

Following gene: 148825438

Centisome position: 16.8

GC content: 39.13

Gene sequence:

>1293_bases
GTGTATTTAAAACAAAATCTAATGAGTTATTTAGCGCAGGCAATAGGCGAGTTAAAATCGAAAATTCACGCATATTTACA
GCAATCAACGAATGAACTTGTCCGTTTTCAGGTGAAACTGGACAAGGTCGATTTATTGGCTTGGCTGAAAAGTCAATCTG
CGTATCCACAATTTTATTTACATTTTCGTGATGAAGAAAAAGCATTGGCTGCGCTTGGCGAAGTGCAGTCATTTTCACAA
TTAAATTTAGCGCAAGAGTTTATTGAAGAAAGTGGTTTCCCACTGGTAGGTGGTTTGCAATTTCAAGGTACCGCACAATT
TGTATTGCCAAAAATTCTTGTTGAACAGGATGAGAAAGGTGCATTGGTATCCTTTTTTGTGAAAGATGAGCAAAGTGCGA
ATGATACTTTAGCCCACCTAAAAACTTTTGAAAATATCACCGCACTTTCTGCTTTGCCAAAACAAATTCCTTTGCATACA
GAGCCTCGAGCGAATGAAAGAACTTGGTGCGATTGGGTAAATCAGGCTTTGGTAGAAATAAAAAGTGGGGAACTGACAAA
AATTGTATTAGCCAATGAAACCACTTTTCATTTAAAGCAAGCGATTAATGCGTACGATTTTTTAGCAGAAAGCGAAAAAC
AAAATCAGGGTTGTTATCATTTTTTGTGGGCTGAAAATCCTTATTCGGTTTTTGTTGGTTCTACGCCAGAACGCCTATTT
GCTCGTGAGTATAACTTGTTGCTAACTGAAGCTTTGGCGGGGACAGCATCGGTTTCTGAAAGTGAGGAAGAAACGCAATC
TCAAGCCAATTGGTTGTTAAATGACGAGAAAAATTTAAAAGAAAATTGGTTGGTCGTAGAAGATATTTCGCAGAATTTAC
GTCAGCAAGTAGAAAGTTTTGATGTAAGCAATGTTGAATTGAAGCCGTTACGTAAAGTACAACATTTGATTCGTAAAATT
CGTGCAAATTTGACCGCACATTATGCGGATGTAAATATATTAAAAGCGATTCATCCTACAGCTGCAGTATCTGGTTTGCC
ACAGCAACAAGCCAAAATGATTTTGTCAGAAATTGAAACCTTTGATCGAGGCTGGTATGCGGGAACATTGGGTGTGATGA
GCGATGTTGGTTCCCAGTTTTGTGTGGCGATTCGTTCTGCCTTTATTGAAGCTCATCGTATTCGTGTATTTGCTGGCGCA
GGCATTGTGGCAGGCTCGCAGCCATTGGAAGAGTGGAAAGAAATTGAACGTAAAGCAGCAGGGTTGATTTCCTTGTTTGC
AGAAGAAAAATGA

Upstream 100 bases:

>100_bases
GAAAAGTGCGGTTAATTTACGCTGAATTTTCAATATTGAAAAGATTTTGTAAAAAATTTTTTGTCATTAACAATCTGCGG
TAGAATACGGCTAATTTTTT

Downstream 100 bases:

>100_bases
TAACGGAGAAAAAAATGTCGGTAAGCGTGTTTAATCGTTGTTGGTCGAAAGTGATTTTAGAAACCTTGGTGCGTCAAGGC
GTTTCTCACGTTTGTATCGC

Product: menaquinone-specific isochorismate synthase

Products: NA

Alternate protein names: Isochorismate mutase [H]

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MYLKQNLMSYLAQAIGELKSKIHAYLQQSTNELVRFQVKLDKVDLLAWLKSQSAYPQFYLHFRDEEKALAALGEVQSFSQ
LNLAQEFIEESGFPLVGGLQFQGTAQFVLPKILVEQDEKGALVSFFVKDEQSANDTLAHLKTFENITALSALPKQIPLHT
EPRANERTWCDWVNQALVEIKSGELTKIVLANETTFHLKQAINAYDFLAESEKQNQGCYHFLWAENPYSVFVGSTPERLF
AREYNLLLTEALAGTASVSESEEETQSQANWLLNDEKNLKENWLVVEDISQNLRQQVESFDVSNVELKPLRKVQHLIRKI
RANLTAHYADVNILKAIHPTAAVSGLPQQQAKMILSEIETFDRGWYAGTLGVMSDVGSQFCVAIRSAFIEAHRIRVFAGA
GIVAGSQPLEEWKEIERKAAGLISLFAEEK

Sequences:

>Translated_430_residues
MYLKQNLMSYLAQAIGELKSKIHAYLQQSTNELVRFQVKLDKVDLLAWLKSQSAYPQFYLHFRDEEKALAALGEVQSFSQ
LNLAQEFIEESGFPLVGGLQFQGTAQFVLPKILVEQDEKGALVSFFVKDEQSANDTLAHLKTFENITALSALPKQIPLHT
EPRANERTWCDWVNQALVEIKSGELTKIVLANETTFHLKQAINAYDFLAESEKQNQGCYHFLWAENPYSVFVGSTPERLF
AREYNLLLTEALAGTASVSESEEETQSQANWLLNDEKNLKENWLVVEDISQNLRQQVESFDVSNVELKPLRKVQHLIRKI
RANLTAHYADVNILKAIHPTAAVSGLPQQQAKMILSEIETFDRGWYAGTLGVMSDVGSQFCVAIRSAFIEAHRIRVFAGA
GIVAGSQPLEEWKEIERKAAGLISLFAEEK
>Mature_430_residues
MYLKQNLMSYLAQAIGELKSKIHAYLQQSTNELVRFQVKLDKVDLLAWLKSQSAYPQFYLHFRDEEKALAALGEVQSFSQ
LNLAQEFIEESGFPLVGGLQFQGTAQFVLPKILVEQDEKGALVSFFVKDEQSANDTLAHLKTFENITALSALPKQIPLHT
EPRANERTWCDWVNQALVEIKSGELTKIVLANETTFHLKQAINAYDFLAESEKQNQGCYHFLWAENPYSVFVGSTPERLF
AREYNLLLTEALAGTASVSESEEETQSQANWLLNDEKNLKENWLVVEDISQNLRQQVESFDVSNVELKPLRKVQHLIRKI
RANLTAHYADVNILKAIHPTAAVSGLPQQQAKMILSEIETFDRGWYAGTLGVMSDVGSQFCVAIRSAFIEAHRIRVFAGA
GIVAGSQPLEEWKEIERKAAGLISLFAEEK

Specific function: Menaquinone biosynthesis. [C]

COG id: COG1169

COG function: function code HQ; Isochorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismate synthase family [H]

Homologues:

Organism=Escherichia coli, GI87082077, Length=409, Percent_Identity=37.8973105134474, Blast_Score=244, Evalue=9e-66,
Organism=Escherichia coli, GI1786809, Length=285, Percent_Identity=27.719298245614, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1788114, Length=240, Percent_Identity=25.8333333333333, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787518, Length=260, Percent_Identity=24.2307692307692, Blast_Score=72, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6320935, Length=194, Percent_Identity=27.319587628866, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR015890
- InterPro:   IPR004561 [H]

Pfam domain/function: PF00425 Chorismate_bind [H]

EC number: =5.4.4.2 [H]

Molecular weight: Translated: 48510; Mature: 48510

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLKQNLMSYLAQAIGELKSKIHAYLQQSTNELVRFQVKLDKVDLLAWLKSQSAYPQFYL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEHHHHHHHHHHHCCCCCCEEEE
HFRDEEKALAALGEVQSFSQLNLAQEFIEESGFPLVGGLQFQGTAQFVLPKILVEQDEKG
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECEEECCCHHHHHHHHHHCCCCCC
ALVSFFVKDEQSANDTLAHLKTFENITALSALPKQIPLHTEPRANERTWCDWVNQALVEI
CEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHC
KSGELTKIVLANETTFHLKQAINAYDFLAESEKQNQGCYHFLWAENPYSVFVGSTPERLF
CCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEEECCCHHHHH
AREYNLLLTEALAGTASVSESEEETQSQANWLLNDEKNLKENWLVVEDISQNLRQQVESF
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCHHCCEEHHHHHHHHHHHHHHC
DVSNVELKPLRKVQHLIRKIRANLTAHYADVNILKAIHPTAAVSGLPQQQAKMILSEIET
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCHHHHHHHHHHHHH
FDRGWYAGTLGVMSDVGSQFCVAIRSAFIEAHRIRVFAGAGIVAGSQPLEEWKEIERKAA
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHHHHHH
GLISLFAEEK
HHHHHHHCCC
>Mature Secondary Structure
MYLKQNLMSYLAQAIGELKSKIHAYLQQSTNELVRFQVKLDKVDLLAWLKSQSAYPQFYL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEHHHHHHHHHHHCCCCCCEEEE
HFRDEEKALAALGEVQSFSQLNLAQEFIEESGFPLVGGLQFQGTAQFVLPKILVEQDEKG
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECEEECCCHHHHHHHHHHCCCCCC
ALVSFFVKDEQSANDTLAHLKTFENITALSALPKQIPLHTEPRANERTWCDWVNQALVEI
CEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHC
KSGELTKIVLANETTFHLKQAINAYDFLAESEKQNQGCYHFLWAENPYSVFVGSTPERLF
CCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEEECCCHHHHH
AREYNLLLTEALAGTASVSESEEETQSQANWLLNDEKNLKENWLVVEDISQNLRQQVESF
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCHHCCEEHHHHHHHHHHHHHHC
DVSNVELKPLRKVQHLIRKIRANLTAHYADVNILKAIHPTAAVSGLPQQQAKMILSEIET
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCHHHHHHHHHHHHH
FDRGWYAGTLGVMSDVGSQFCVAIRSAFIEAHRIRVFAGAGIVAGSQPLEEWKEIERKAA
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHHHHHH
GLISLFAEEK
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]