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The map label for this gene is holB [H]

Identifier: 148825265

GI number: 148825265

Start: 137201

End: 138184

Strand: Direct

Name: holB [H]

Synonym: CGSHiEE_00715

Alternate gene names: 148825265

Gene position: 137201-138184 (Clockwise)

Preceding gene: 148825264

Following gene: 148825266

Centisome position: 7.57

GC content: 40.65

Gene sequence:

>984_bases
ATGACCGCACTTTACCCTTGGCTAACGCCAATTTATCATCAAATTGCTCAAACCTTTGACGAAGGGTTGGGGCATCATGC
TGTGCTAATTAAAGCTGATTCTGGTTTAGGCGTAGAGAGTTTATTTAATGCACTTGCACAGAAAATAATGTGTGTAGCTC
AAGGCGATAAACCTTGTGGTCAATGCCATTCTTGTCATTTAATGCAAGCCCATAGCCATCCAGATTATCACGAATTAAGC
CCCATTGACGGTAAGGATATTGGCGTTGATCAAGTACGCGACATTAATGAAATTGTTGCGCAGCACGCACAACAAAACGG
CAATAAAGTGGTGTATGTGCAAGGGGTGGAACGTTTAACGGAAGCGGCTGCTAATGCATTATTGAAAACATTGGAAGAGC
CTCGTCCAAATACTTATTTTTTGCTTCAAGCGGATAGTTCGGCAAGTTTGTTAGCAACTATTTACAGTCGATGCCAAGTG
TGGAATCTTTCCGTGCCTAATGAACAAACGGCTGTTGATTGGTTGAAATCAAAAAGTGCGGTAGAAAATCAGGAAATTTT
GACCGCACTTGCGATGAATCTTGGAAGACCGCTTTTAGCATTAGAAACGTTACAAGAAGGATTTATTGAACAGCGTAAAA
ACTTCTTACGTCAATTTTGGGTGTTCTATCGCCGACGTTCGCCATTGGAATTGCTTCCGTTGTTTGACAAAGAACGCTAT
GTTCAGCAAGTGGATTGGATTTTGGCTTTTCTTTCTGATTGTTTAAAACATAAACTTGAAATTGATAGTCATCGACAAGT
TGCGGATCTCGGGCGTGGCATCGAACAATTCAGTAATGAGCAAACTGCCCTTGGTTTATTACAAGCAATCAAAATTATGC
AACAAGTGCGGTCAGATTTGCTGACTATTAATGGCGTGAATGTTGAATTGATGCTATTAGATGGCTTGATGCGATTAGTC
ACAGAAGTATTTGAAACGCAATAA

Upstream 100 bases:

>100_bases
ATAATCCAAAAGCAGTAATGATTAATGCAGAGCAGAGTATTGAACTTGTTCAAGCTGATATCGAAAGAGCGGTTAAAAAT
TGGTGGAAATCAAACGAAAA

Downstream 100 bases:

>100_bases
ATTAAAGATATATCGTCTATGGCGTTAAAGCCTTAGGCATAAGGAAAATAATTATGTTCATTGTTGATTCCCACTGCCAT
TTAGATGCGTTGGATTATGA

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MTALYPWLTPIYHQIAQTFDEGLGHHAVLIKADSGLGVESLFNALAQKIMCVAQGDKPCGQCHSCHLMQAHSHPDYHELS
PIDGKDIGVDQVRDINEIVAQHAQQNGNKVVYVQGVERLTEAAANALLKTLEEPRPNTYFLLQADSSASLLATIYSRCQV
WNLSVPNEQTAVDWLKSKSAVENQEILTALAMNLGRPLLALETLQEGFIEQRKNFLRQFWVFYRRRSPLELLPLFDKERY
VQQVDWILAFLSDCLKHKLEIDSHRQVADLGRGIEQFSNEQTALGLLQAIKIMQQVRSDLLTINGVNVELMLLDGLMRLV
TEVFETQ

Sequences:

>Translated_327_residues
MTALYPWLTPIYHQIAQTFDEGLGHHAVLIKADSGLGVESLFNALAQKIMCVAQGDKPCGQCHSCHLMQAHSHPDYHELS
PIDGKDIGVDQVRDINEIVAQHAQQNGNKVVYVQGVERLTEAAANALLKTLEEPRPNTYFLLQADSSASLLATIYSRCQV
WNLSVPNEQTAVDWLKSKSAVENQEILTALAMNLGRPLLALETLQEGFIEQRKNFLRQFWVFYRRRSPLELLPLFDKERY
VQQVDWILAFLSDCLKHKLEIDSHRQVADLGRGIEQFSNEQTALGLLQAIKIMQQVRSDLLTINGVNVELMLLDGLMRLV
TEVFETQ
>Mature_326_residues
TALYPWLTPIYHQIAQTFDEGLGHHAVLIKADSGLGVESLFNALAQKIMCVAQGDKPCGQCHSCHLMQAHSHPDYHELSP
IDGKDIGVDQVRDINEIVAQHAQQNGNKVVYVQGVERLTEAAANALLKTLEEPRPNTYFLLQADSSASLLATIYSRCQVW
NLSVPNEQTAVDWLKSKSAVENQEILTALAMNLGRPLLALETLQEGFIEQRKNFLRQFWVFYRRRSPLELLPLFDKERYV
QQVDWILAFLSDCLKHKLEIDSHRQVADLGRGIEQFSNEQTALGLLQAIKIMQQVRSDLLTINGVNVELMLLDGLMRLVT
EVFETQ

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787341, Length=317, Percent_Identity=35.9621451104101, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI1786676, Length=151, Percent_Identity=33.112582781457, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008921
- InterPro:   IPR004622
- InterPro:   IPR015199 [H]

Pfam domain/function: PF09115 DNApol3-delta_C [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 36974; Mature: 36843

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTALYPWLTPIYHQIAQTFDEGLGHHAVLIKADSGLGVESLFNALAQKIMCVAQGDKPCG
CCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCC
QCHSCHLMQAHSHPDYHELSPIDGKDIGVDQVRDINEIVAQHAQQNGNKVVYVQGVERLT
CCCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHH
EAAANALLKTLEEPRPNTYFLLQADSSASLLATIYSRCQVWNLSVPNEQTAVDWLKSKSA
HHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHH
VENQEILTALAMNLGRPLLALETLQEGFIEQRKNFLRQFWVFYRRRSPLELLPLFDKERY
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHH
VQQVDWILAFLSDCLKHKLEIDSHRQVADLGRGIEQFSNEQTALGLLQAIKIMQQVRSDL
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
LTINGVNVELMLLDGLMRLVTEVFETQ
EEECCCCEEHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TALYPWLTPIYHQIAQTFDEGLGHHAVLIKADSGLGVESLFNALAQKIMCVAQGDKPCG
CCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCC
QCHSCHLMQAHSHPDYHELSPIDGKDIGVDQVRDINEIVAQHAQQNGNKVVYVQGVERLT
CCCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHH
EAAANALLKTLEEPRPNTYFLLQADSSASLLATIYSRCQVWNLSVPNEQTAVDWLKSKSA
HHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHH
VENQEILTALAMNLGRPLLALETLQEGFIEQRKNFLRQFWVFYRRRSPLELLPLFDKERY
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHH
VQQVDWILAFLSDCLKHKLEIDSHRQVADLGRGIEQFSNEQTALGLLQAIKIMQQVRSDL
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
LTINGVNVELMLLDGLMRLVTEVFETQ
EEECCCCEEHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]