Click here to switch to the map view.

The map label for this gene is tmk

Identifier: 148825264

GI number: 148825264

Start: 136572

End: 137204

Strand: Direct

Name: tmk

Synonym: CGSHiEE_00710

Alternate gene names: 148825264

Gene position: 136572-137204 (Clockwise)

Preceding gene: 148825263

Following gene: 148825265

Centisome position: 7.53

GC content: 40.76

Gene sequence:

>633_bases
ATGAAAGGAAAATTTATTGTCATTGAAGGTTTGGAAGGTGCGGGGAAAAGTTCTGCTCATCAGTCTGTTGTGCGAGTTTT
GCATGAACTTGCTATTCAAGATGTCGTATTTACACGCGAGCCTGGTGGAACACCATTGGCAGAGAAATTACGTCATCTCA
TCAAACATGAAACCGAAGAACCCGTGACACATAAAGCAGAGTTATTAATGCTTTATGCGGCTCGTATTCAGTTGGTGGAT
AACGTGATTAAACCTGCTTTAATGCAAGGAAAATGGGTGGTAGGTGATCGCCACGATATGTCATCTCAGGCGTATCAAGG
TGGCGGACGTCAATTAGACCCTCATTTTATGCTCACCTTGAAAGAAACCGTATTAGGTGATTTTGAGCCAGATCTCACAA
TTTATTTGGATATAGATCCGATTGTCGGTTTAGCGCGAGCTCGTGGACGTGGTGAATTAGATCGTATTGAGCAAATGGAT
TTGGATTTTTTTCACCGTACTCGAGCACGCTATTTAGAGTTAGTAAAAGATAATCCAAAAGCAGTAATGATTAATGCAGA
GCAGAGTATTGAACTTGTTCAAGCTGATATCGAAAGAGCGGTTAAAAATTGGTGGAAATCAAACGAAAAATGA

Upstream 100 bases:

>100_bases
AAATTTACCCGAAATTTGAATGAACATAACAAAGCGGTGCAAGAATATTTACGCTGGTATCGCAGTCAGAAAAATGCCAA
ATAAAAGTGCGGAAGAAAAT

Downstream 100 bases:

>100_bases
CCGCACTTTACCCTTGGCTAACGCCAATTTATCATCAAATTGCTCAAACCTTTGACGAAGGGTTGGGGCATCATGCTGTG
CTAATTAAAGCTGATTCTGG

Product: thymidylate kinase

Products: NA

Alternate protein names: dTMP kinase

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MKGKFIVIEGLEGAGKSSAHQSVVRVLHELAIQDVVFTREPGGTPLAEKLRHLIKHETEEPVTHKAELLMLYAARIQLVD
NVIKPALMQGKWVVGDRHDMSSQAYQGGGRQLDPHFMLTLKETVLGDFEPDLTIYLDIDPIVGLARARGRGELDRIEQMD
LDFFHRTRARYLELVKDNPKAVMINAEQSIELVQADIERAVKNWWKSNEK

Sequences:

>Translated_210_residues
MKGKFIVIEGLEGAGKSSAHQSVVRVLHELAIQDVVFTREPGGTPLAEKLRHLIKHETEEPVTHKAELLMLYAARIQLVD
NVIKPALMQGKWVVGDRHDMSSQAYQGGGRQLDPHFMLTLKETVLGDFEPDLTIYLDIDPIVGLARARGRGELDRIEQMD
LDFFHRTRARYLELVKDNPKAVMINAEQSIELVQADIERAVKNWWKSNEK
>Mature_210_residues
MKGKFIVIEGLEGAGKSSAHQSVVRVLHELAIQDVVFTREPGGTPLAEKLRHLIKHETEEPVTHKAELLMLYAARIQLVD
NVIKPALMQGKWVVGDRHDMSSQAYQGGGRQLDPHFMLTLKETVLGDFEPDLTIYLDIDPIVGLARARGRGELDRIEQMD
LDFFHRTRARYLELVKDNPKAVMINAEQSIELVQADIERAVKNWWKSNEK

Specific function: Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis

COG id: COG0125

COG function: function code F; Thymidylate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate kinase family

Homologues:

Organism=Escherichia coli, GI1787340, Length=209, Percent_Identity=61.244019138756, Blast_Score=259, Evalue=1e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KTHY_HAEIE (A5UA33)

Other databases:

- EMBL:   CP000671
- RefSeq:   YP_001290017.1
- ProteinModelPortal:   A5UA33
- SMR:   A5UA33
- STRING:   A5UA33
- GeneID:   5225277
- GenomeReviews:   CP000671_GR
- KEGG:   hip:CGSHiEE_00710
- eggNOG:   COG0125
- HOGENOM:   HBG626009
- OMA:   LFITLEG
- ProtClustDB:   PRK00698
- BioCyc:   HINF374930:CGSHIEE_00710-MONOMER
- HAMAP:   MF_00165
- InterPro:   IPR018095
- InterPro:   IPR018094
- PANTHER:   PTHR10344
- TIGRFAMs:   TIGR00041

Pfam domain/function: PF02223 Thymidylate_kin

EC number: =2.7.4.9

Molecular weight: Translated: 23844; Mature: 23844

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS01331 THYMIDYLATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGKFIVIEGLEGAGKSSAHQSVVRVLHELAIQDVVFTREPGGTPLAEKLRHLIKHETEE
CCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHCCCCC
PVTHKAELLMLYAARIQLVDNVIKPALMQGKWVVGDRHDMSSQAYQGGGRQLDPHFMLTL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHCCCCCCCCCHHEEEE
KETVLGDFEPDLTIYLDIDPIVGLARARGRGELDRIEQMDLDFFHRTRARYLELVKDNPK
HHHHCCCCCCCEEEEEECCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AVMINAEQSIELVQADIERAVKNWWKSNEK
EEEEECHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKGKFIVIEGLEGAGKSSAHQSVVRVLHELAIQDVVFTREPGGTPLAEKLRHLIKHETEE
CCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHCCCCC
PVTHKAELLMLYAARIQLVDNVIKPALMQGKWVVGDRHDMSSQAYQGGGRQLDPHFMLTL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHCCCCCCCCCHHEEEE
KETVLGDFEPDLTIYLDIDPIVGLARARGRGELDRIEQMDLDFFHRTRARYLELVKDNPK
HHHHCCCCCCCEEEEEECCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AVMINAEQSIELVQADIERAVKNWWKSNEK
EEEEECHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA