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The map label for this gene is rlmN

Identifier: 148824069

GI number: 148824069

Start: 3201113

End: 3202207

Strand: Reverse

Name: rlmN

Synonym: TBFG_12894

Alternate gene names: 148824069

Gene position: 3202207-3201113 (Counterclockwise)

Preceding gene: 148824070

Following gene: 148824068

Centisome position: 72.38

GC content: 66.67

Gene sequence:

>1095_bases
ATGGTCCCCGAGTTGATGTTCGATGAGCCGCGTCCGGGTCGGCCGCCGCGGCACCTGGCCGATCTCGACGCGGCGGGCCG
AGCGTCTGCCGTCGCGGAGCTGGGGCTGCCGGCGTTTCGGGCCAAGCAGCTTGCACACCAGTACTACGGTCGGCTGATCG
CCGATCCGCGTCAGATGACCGACCTTCCGGCGGCCGTTCGCGACCGGATCGCCGGAGCGATGTTCCCGAACTTGCTTACC
GCGTCCGCCGACATCACCTGCGATGCCGGCCAGACTCGAAAGACGTTGTGGCGGGCCGTCGACGGTACCATGTTCGAATC
GGTGCTGATGCGCTATCCGCGGCGCAACACGGTCTGCATTTCCTCGCAGGCCGGCTGCGGCATGGCTTGCCCGTTCTGTG
CCACTGGCCAGGGCGGATTGACTCGCAACCTATCGACGGCGGAGATCCTCGAGCAGGTGCGTGCCGGCGCCGCGGCGTTG
CGCGACGACTTCGGCGATCGGTTGTCGAATGTGGTGTTCATGGGTATGGGGGAGCCGCTGGCCAACTACGCCAGGGTGTT
GGCCGCAGTTCAGCGCATTACCGCGCGGCCGCCGTCCGGTTTCGGGATTTCGGCCCGCGCGGTGACGGTGTCGACGGTGG
GTCTGGCCCCTGCTATCCGCAACCTTGCCGACGCGCGGCTCGGCGTGACCCTGGCGCTGTCGCTGCACGCCCCCGACGAC
GGGTTGCGCGATACACTAGTTCCGGTCAACAACCGGTGGAGGATCAGCGAAGCGCTCGATGCGGCCCGGTACTACGCCAA
TGTGACCGGGCGACGGGTGTCTATTGAGTACGCGCTGATCCGTGATGTCAACGACCAACCGTGGCGGGCCGACTTGCTGG
GAAAGCGACTGCATCGTGTTCTCGGGCCGTTGGCGCACGTGAACCTGATCCCGCTGAACCCGACTCCGGGCAGTGATTGG
GATGCCAGCCCCAAGCCGGTTGAGCGTGAATTCGTCAAACGGGTTCGGGCGAAAGGAGTTTCCTGCACGGTACGAGACAC
CCGTGGGCGGGAGATCAGCGCCGCCTGCGGACAGCTGGCCGCCGTAGGCGGGTAG

Upstream 100 bases:

>100_bases
GGCGGTCTGATGGACCGGCTCGACGGCATACTGCCTTCCGCGGTGGCGGCCTGGATAGTCCTCACACTGCTGCCCTGACA
ACGGCGATACTGGACAGGTC

Downstream 100 bases:

>100_bases
CTAGAGCGCAGGCGAATCAGCCAGCGCTGCGGACAGCTGGCCGCCGTAGGCGGGTAGCTGGAGCGCAGGCGAATCAGCCA
GCGCTGCGGACAGCTGGCCG

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MVPELMFDEPRPGRPPRHLADLDAAGRASAVAELGLPAFRAKQLAHQYYGRLIADPRQMTDLPAAVRDRIAGAMFPNLLT
ASADITCDAGQTRKTLWRAVDGTMFESVLMRYPRRNTVCISSQAGCGMACPFCATGQGGLTRNLSTAEILEQVRAGAAAL
RDDFGDRLSNVVFMGMGEPLANYARVLAAVQRITARPPSGFGISARAVTVSTVGLAPAIRNLADARLGVTLALSLHAPDD
GLRDTLVPVNNRWRISEALDAARYYANVTGRRVSIEYALIRDVNDQPWRADLLGKRLHRVLGPLAHVNLIPLNPTPGSDW
DASPKPVEREFVKRVRAKGVSCTVRDTRGREISAACGQLAAVGG

Sequences:

>Translated_364_residues
MVPELMFDEPRPGRPPRHLADLDAAGRASAVAELGLPAFRAKQLAHQYYGRLIADPRQMTDLPAAVRDRIAGAMFPNLLT
ASADITCDAGQTRKTLWRAVDGTMFESVLMRYPRRNTVCISSQAGCGMACPFCATGQGGLTRNLSTAEILEQVRAGAAAL
RDDFGDRLSNVVFMGMGEPLANYARVLAAVQRITARPPSGFGISARAVTVSTVGLAPAIRNLADARLGVTLALSLHAPDD
GLRDTLVPVNNRWRISEALDAARYYANVTGRRVSIEYALIRDVNDQPWRADLLGKRLHRVLGPLAHVNLIPLNPTPGSDW
DASPKPVEREFVKRVRAKGVSCTVRDTRGREISAACGQLAAVGG
>Mature_364_residues
MVPELMFDEPRPGRPPRHLADLDAAGRASAVAELGLPAFRAKQLAHQYYGRLIADPRQMTDLPAAVRDRIAGAMFPNLLT
ASADITCDAGQTRKTLWRAVDGTMFESVLMRYPRRNTVCISSQAGCGMACPFCATGQGGLTRNLSTAEILEQVRAGAAAL
RDDFGDRLSNVVFMGMGEPLANYARVLAAVQRITARPPSGFGISARAVTVSTVGLAPAIRNLADARLGVTLALSLHAPDD
GLRDTLVPVNNRWRISEALDAARYYANVTGRRVSIEYALIRDVNDQPWRADLLGKRLHRVLGPLAHVNLIPLNPTPGSDW
DASPKPVEREFVKRVRAKGVSCTVRDTRGREISAACGQLAAVGG

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=352, Percent_Identity=36.9318181818182, Blast_Score=191, Evalue=5e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_MYCBO (P0A645)

Other databases:

- EMBL:   BX248344
- RefSeq:   NP_856549.1
- ProteinModelPortal:   P0A645
- EnsemblBacteria:   EBMYCT00000014366
- GeneID:   1092204
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb2904c
- GeneTree:   EBGT00050000015756
- HOGENOM:   HBG335345
- OMA:   DINDQPW
- ProtClustDB:   PRK14459
- BioCyc:   MBOV233413:MB2904C-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 39256; Mature: 39256

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPELMFDEPRPGRPPRHLADLDAAGRASAVAELGLPAFRAKQLAHQYYGRLIADPRQMT
CCCCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHH
DLPAAVRDRIAGAMFPNLLTASADITCDAGQTRKTLWRAVDGTMFESVLMRYPRRNTVCI
HCCHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
SSQAGCGMACPFCATGQGGLTRNLSTAEILEQVRAGAAALRDDFGDRLSNVVFMGMGEPL
ECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHH
ANYARVLAAVQRITARPPSGFGISARAVTVSTVGLAPAIRNLADARLGVTLALSLHAPDD
HHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEHHHHHHHHHHHHHHHCCEEEEEEEECCCC
GLRDTLVPVNNRWRISEALDAARYYANVTGRRVSIEYALIRDVNDQPWRADLLGKRLHRV
CCCCCCCCCCCCEEHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHH
LGPLAHVNLIPLNPTPGSDWDASPKPVEREFVKRVRAKGVSCTVRDTRGREISAACGQLA
HCHHHHEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHH
AVGG
HCCC
>Mature Secondary Structure
MVPELMFDEPRPGRPPRHLADLDAAGRASAVAELGLPAFRAKQLAHQYYGRLIADPRQMT
CCCCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHH
DLPAAVRDRIAGAMFPNLLTASADITCDAGQTRKTLWRAVDGTMFESVLMRYPRRNTVCI
HCCHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
SSQAGCGMACPFCATGQGGLTRNLSTAEILEQVRAGAAALRDDFGDRLSNVVFMGMGEPL
ECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHH
ANYARVLAAVQRITARPPSGFGISARAVTVSTVGLAPAIRNLADARLGVTLALSLHAPDD
HHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEHHHHHHHHHHHHHHHCCEEEEEEEECCCC
GLRDTLVPVNNRWRISEALDAARYYANVTGRRVSIEYALIRDVNDQPWRADLLGKRLHRV
CCCCCCCCCCCCEEHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHH
LGPLAHVNLIPLNPTPGSDWDASPKPVEREFVKRVRAKGVSCTVRDTRGREISAACGQLA
HCHHHHEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHH
AVGG
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972