Click here to switch to the map view.

The map label for this gene is fadE30 [H]

Identifier: 148663423

GI number: 148663423

Start: 4009098

End: 4010255

Strand: Reverse

Name: fadE30 [H]

Synonym: MRA_3599

Alternate gene names: 148663423

Gene position: 4010255-4009098 (Counterclockwise)

Preceding gene: 148663429

Following gene: 148663422

Centisome position: 90.73

GC content: 68.22

Gene sequence:

>1158_bases
ATGCAGGACGTCGAGGAGTTCCGGGCGCAGGTCCGCGGTTGGCTCGCCGACAATCTGGCCGGCGAATTCGCGGCACTTAA
GGGCCTCGGCGGGCCGGGGCGCGAGCACGAAGCGTTCGAGGAACGCCGGGCGTGGAATCAGCGCCTGGCCGCCGCGGGGC
TGACCTGCCTGGGCTGGCCGGAAGAACACGGCGGGCGGGGGCTCTCGACCGCGCACCGGGTGGCGTTCTACGAGGAATAC
GCCCGCGCCGACGCGCCGGACAAGGTCAACCACTTCGGCGAGGAACTGCTGGGTCCGACGCTGATCGCGTTCGGAACGCC
GCAGCAGCAGCGGCGCTTCCTGCCGCGCATCCGCGACGTCACCGAGCTGTGGTGTCAGGGATATTCGGAGCCCGGCGCTG
GCAGCGACCTGGCCAGCGTTGCGACCACCGCAGAACTCGACGGCGACCAGTGGGTGATAAACGGCCAGAAGGTGTGGACG
TCGCTGGCGCACCTGTCGCAATGGTGCTTTGTGCTCGCACGCACCGAGAAGGGCTCGCAGCGCCACGCCGGCCTGTCGTA
TCTGCTGGTGCCGCTGGACCAGCCCGGCGTGCAGATCCGGCCGATCGTGCAGATCACCGGCACCGCGGAGTTCAACGAGG
TGTTCTTCGACGACGCCCGCACCGACGCCGACCTGGTGGTCGGCGCACCGGGCGACGGCTGGCGGGTCGCGATGGCGACG
CTGACCTTCGAGCGCGGCGTCTCGACGCTGGGTCAGCAGATCGTCTACGCCCGTGAGCTTTCCAATCTGGTCGAGCTGGC
GCGGCGCACCGCCGCCGCGGATGACCCGCTGATCCGGGAGCGGCTGACCCGGGCGTGGACCGGGCTGCGCGCCATGCGCT
CCTATGCTTTGGCCACCATGGAAGGGCCAGCCGTTGAGCAGCCCGGCCAGGACAATGTGTCAAAGTTGTTGTGGGCCAAC
TGGCATCGCAACCTGGGTGAGCTGGCCATGGACGTGATCGGTAAACCCGGAATGACCATGCCCGACGGCGAGTTCGACGA
GTGGCAGCGGCTATATCTATTCACCCGCGCCGACACCATCTACGGAGGATCCAACGAGATCCAGCGCAACATCATCGCCG
AGCGGGTGCTCGGTCTGCCCCGGGAGGCCAAGGGATGA

Upstream 100 bases:

>100_bases
TGGCGCACGAGACGATCCAGCGCCGCGGGCACGGTGCGCAGATCGTTTATCAACGGACGCTCCTAACAAAGCAAGTGCTT
GGTAGGTTAGCCTACAGGGC

Downstream 100 bases:

>100_bases
ACCTGTCCGTAGCGCCGAAAGAGATTGCCGGACATGGACTTCTGGATGGCAAGGTGGTCGTCGTGACGGCGGCTGCGGGC
ACCGGCATCGGTTCGGCCAC

Product: acyl-CoA dehydrogenase FadE30

Products: gamma-butyrobetaine; Acceptor; gamma-butyrobetainyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MQDVEEFRAQVRGWLADNLAGEFAALKGLGGPGREHEAFEERRAWNQRLAAAGLTCLGWPEEHGGRGLSTAHRVAFYEEY
ARADAPDKVNHFGEELLGPTLIAFGTPQQQRRFLPRIRDVTELWCQGYSEPGAGSDLASVATTAELDGDQWVINGQKVWT
SLAHLSQWCFVLARTEKGSQRHAGLSYLLVPLDQPGVQIRPIVQITGTAEFNEVFFDDARTDADLVVGAPGDGWRVAMAT
LTFERGVSTLGQQIVYARELSNLVELARRTAAADDPLIRERLTRAWTGLRAMRSYALATMEGPAVEQPGQDNVSKLLWAN
WHRNLGELAMDVIGKPGMTMPDGEFDEWQRLYLFTRADTIYGGSNEIQRNIIAERVLGLPREAKG

Sequences:

>Translated_385_residues
MQDVEEFRAQVRGWLADNLAGEFAALKGLGGPGREHEAFEERRAWNQRLAAAGLTCLGWPEEHGGRGLSTAHRVAFYEEY
ARADAPDKVNHFGEELLGPTLIAFGTPQQQRRFLPRIRDVTELWCQGYSEPGAGSDLASVATTAELDGDQWVINGQKVWT
SLAHLSQWCFVLARTEKGSQRHAGLSYLLVPLDQPGVQIRPIVQITGTAEFNEVFFDDARTDADLVVGAPGDGWRVAMAT
LTFERGVSTLGQQIVYARELSNLVELARRTAAADDPLIRERLTRAWTGLRAMRSYALATMEGPAVEQPGQDNVSKLLWAN
WHRNLGELAMDVIGKPGMTMPDGEFDEWQRLYLFTRADTIYGGSNEIQRNIIAERVLGLPREAKG
>Mature_385_residues
MQDVEEFRAQVRGWLADNLAGEFAALKGLGGPGREHEAFEERRAWNQRLAAAGLTCLGWPEEHGGRGLSTAHRVAFYEEY
ARADAPDKVNHFGEELLGPTLIAFGTPQQQRRFLPRIRDVTELWCQGYSEPGAGSDLASVATTAELDGDQWVINGQKVWT
SLAHLSQWCFVLARTEKGSQRHAGLSYLLVPLDQPGVQIRPIVQITGTAEFNEVFFDDARTDADLVVGAPGDGWRVAMAT
LTFERGVSTLGQQIVYARELSNLVELARRTAAADDPLIRERLTRAWTGLRAMRSYALATMEGPAVEQPGQDNVSKLLWAN
WHRNLGELAMDVIGKPGMTMPDGEFDEWQRLYLFTRADTIYGGSNEIQRNIIAERVLGLPREAKG

Specific function: Catalyzes the reduction of crotonobetainyl-CoA to gamma-butyrobetainyl-CoA. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acyl-CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI4557233, Length=349, Percent_Identity=28.6532951289398, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI4557231, Length=347, Percent_Identity=26.8011527377522, Blast_Score=102, Evalue=8e-22,
Organism=Homo sapiens, GI187960098, Length=347, Percent_Identity=26.8011527377522, Blast_Score=101, Evalue=9e-22,
Organism=Homo sapiens, GI4501857, Length=388, Percent_Identity=25, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI21361497, Length=356, Percent_Identity=26.9662921348315, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI226958414, Length=349, Percent_Identity=24.9283667621776, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI226958412, Length=355, Percent_Identity=25.3521126760563, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI4501859, Length=356, Percent_Identity=26.123595505618, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI76496475, Length=355, Percent_Identity=25.0704225352113, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI4557235, Length=355, Percent_Identity=25.0704225352113, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI7656849, Length=327, Percent_Identity=23.5474006116208, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI48976061, Length=198, Percent_Identity=28.7878787878788, Blast_Score=69, Evalue=8e-12,
Organism=Homo sapiens, GI211971008, Length=198, Percent_Identity=28.7878787878788, Blast_Score=69, Evalue=9e-12,
Organism=Escherichia coli, GI1786223, Length=342, Percent_Identity=24.8538011695906, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI87081958, Length=340, Percent_Identity=22.9411764705882, Blast_Score=72, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17570075, Length=389, Percent_Identity=26.9922879177378, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17569725, Length=343, Percent_Identity=26.530612244898, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17534899, Length=190, Percent_Identity=30.5263157894737, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17508101, Length=340, Percent_Identity=28.2352941176471, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17538396, Length=264, Percent_Identity=27.2727272727273, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17506239, Length=346, Percent_Identity=25.7225433526012, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17551932, Length=228, Percent_Identity=27.1929824561404, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17505929, Length=230, Percent_Identity=29.1304347826087, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI32563615, Length=201, Percent_Identity=29.8507462686567, Blast_Score=81, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI71990804, Length=299, Percent_Identity=23.4113712374582, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17533517, Length=192, Percent_Identity=26.5625, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24646207, Length=360, Percent_Identity=28.6111111111111, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI21356377, Length=360, Percent_Identity=25.8333333333333, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI281363737, Length=343, Percent_Identity=26.2390670553936, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24660351, Length=303, Percent_Identity=26.4026402640264, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI21355753, Length=268, Percent_Identity=25.7462686567164, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24666513, Length=238, Percent_Identity=26.0504201680672, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006089
- InterPro:   IPR006092
- InterPro:   IPR006090
- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR013786
- InterPro:   IPR009100 [H]

Pfam domain/function: PF00441 Acyl-CoA_dh_1; PF02770 Acyl-CoA_dh_M; PF02771 Acyl-CoA_dh_N [H]

EC number: 1.3.99.- [C]

Molecular weight: Translated: 42733; Mature: 42733

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDVEEFRAQVRGWLADNLAGEFAALKGLGGPGREHEAFEERRAWNQRLAAAGLTCLGWP
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCEEECCC
EEHGGRGLSTAHRVAFYEEYARADAPDKVNHFGEELLGPTLIAFGTPQQQRRFLPRIRDV
HHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHH
TELWCQGYSEPGAGSDLASVATTAELDGDQWVINGQKVWTSLAHLSQWCFVLARTEKGSQ
HHHHHCCCCCCCCCCHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHCCCCCCC
RHAGLSYLLVPLDQPGVQIRPIVQITGTAEFNEVFFDDARTDADLVVGAPGDGWRVAMAT
HHCCCEEEEEECCCCCCEEEEEEEEECCCHHHHHHHCCCCCCCCEEEECCCCCCEEEHHH
LTFERGVSTLGQQIVYARELSNLVELARRTAAADDPLIRERLTRAWTGLRAMRSYALATM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
EGPAVEQPGQDNVSKLLWANWHRNLGELAMDVIGKPGMTMPDGEFDEWQRLYLFTRADTI
CCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCHHHHHHEEEEEECCEE
YGGSNEIQRNIIAERVLGLPREAKG
ECCHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MQDVEEFRAQVRGWLADNLAGEFAALKGLGGPGREHEAFEERRAWNQRLAAAGLTCLGWP
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCEEECCC
EEHGGRGLSTAHRVAFYEEYARADAPDKVNHFGEELLGPTLIAFGTPQQQRRFLPRIRDV
HHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHH
TELWCQGYSEPGAGSDLASVATTAELDGDQWVINGQKVWTSLAHLSQWCFVLARTEKGSQ
HHHHHCCCCCCCCCCHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHCCCCCCC
RHAGLSYLLVPLDQPGVQIRPIVQITGTAEFNEVFFDDARTDADLVVGAPGDGWRVAMAT
HHCCCEEEEEECCCCCCEEEEEEEEECCCHHHHHHHCCCCCCCCEEEECCCCCCEEEHHH
LTFERGVSTLGQQIVYARELSNLVELARRTAAADDPLIRERLTRAWTGLRAMRSYALATM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
EGPAVEQPGQDNVSKLLWANWHRNLGELAMDVIGKPGMTMPDGEFDEWQRLYLFTRADTI
CCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCHHHHHHEEEEEECCEE
YGGSNEIQRNIIAERVLGLPREAKG
ECCHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: crotono-betaine; reduced acceptor; crotonobetainyl-CoA [C]

Specific reaction: reduced acceptor + crotono-betaine = Acceptor + gamma-butyrobetaine reduced acceptor + crotonobetainyl-CoA = Acceptor + gamma-butyrobetainyl-CoA [C]

General reaction: Oxidoreductases; Acting on the CH-CH group of donors; With other acceptors [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]