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The map label for this gene is nat

Identifier: 148663429

GI number: 148663429

Start: 4015997

End: 4016848

Strand: Reverse

Name: nat

Synonym: MRA_3605

Alternate gene names: 148663429

Gene position: 4016848-4015997 (Counterclockwise)

Preceding gene: 148663430

Following gene: 148663423

Centisome position: 90.88

GC content: 64.91

Gene sequence:

>852_bases
ATGGCACTGGATCTGACCGCGTACTTCGATCGCATCAACTATCGCGGCGCTACCGATCCAACCCTGGATGTTCTGCAGGA
TCTGGTGACCGTGCACAGTCGAACGATTCCGTTCGAGAACCTCGACCCGCTGCTGGGGGTGCCGGTCGACGACCTCAGTC
CACAGGCGCTGGCCGACAAGCTGGTACTTCGGCGCCGAGGCGGGTACTGCTTTGAGCACAACGGGCTGATGGGTTATGTG
CTGGCCGAACTCGGCTATCGGGTGCGCCGATTCGCCGCCCGCGTCGTCTGGAAGCTCGCGCCGGACGCGCCCCTGCCGCC
GCAGACGCACACCCTGCTGGGGGTCACGTTCCCCGGCTCGGGCGGATGCTATCTCGTCGACGTCGGATTCGGCGGCCAAA
CACCGACCTCACCGCTTCGCCTCGAAACCGGCGCCGTCCAGCCGACAACGCACGAACCTTATCGGCTCGAGGACCGCGTC
GACGGCTTTGTCTTGCAGGCGATGGTCCGGGACACATGGCAGACACTGTACGAATTCACCACCCAGACCCGCCCGCAGAT
CGATCTGAAAGTGGCCAGCTGGTACGCCTCAACACACCCGGCATCGAAGTTCGTCACGGGACTGACCGCCGCGGTGATCA
CCGACGACGCCCGGTGGAACCTATCTGGCCGCGACCTTGCCGTTCACCGTGCCGGTGGTACCGAGAAGATCCGCCTTGCC
GATGCGGCAGCGGTTGTCGACACCCTGAGCGAACGGTTCGGGATCAACGTGGCAGATATCGGCGAGCGCGGCGCGCTCGA
GACGCGCATCGACGAGCTATTGGCTCGGCAGCCAGGAGCCGATGCGCCGTAA

Upstream 100 bases:

>100_bases
TGGCAAACCACAATCGGTGCGACATAGTTGGCCGGCTGCGCGACGAACGCCCGAATACCCTGCGGTCGGTCCGACGCGGC
GACGAGGTCAGAATGGCAAC

Downstream 100 bases:

>100_bases
GGCTTCTTCGATGTCGCCGCTTGGCCCGGCAAACGATATCCGAACAAACGAACCCCCCCGTGCGGTGTCGAAATCGATTC
CGGGTGCGATCGCAACACCG

Product: arylamine N-acetyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MALDLTAYFDRINYRGATDPTLDVLQDLVTVHSRTIPFENLDPLLGVPVDDLSPQALADKLVLRRRGGYCFEHNGLMGYV
LAELGYRVRRFAARVVWKLAPDAPLPPQTHTLLGVTFPGSGGCYLVDVGFGGQTPTSPLRLETGAVQPTTHEPYRLEDRV
DGFVLQAMVRDTWQTLYEFTTQTRPQIDLKVASWYASTHPASKFVTGLTAAVITDDARWNLSGRDLAVHRAGGTEKIRLA
DAAAVVDTLSERFGINVADIGERGALETRIDELLARQPGADAP

Sequences:

>Translated_283_residues
MALDLTAYFDRINYRGATDPTLDVLQDLVTVHSRTIPFENLDPLLGVPVDDLSPQALADKLVLRRRGGYCFEHNGLMGYV
LAELGYRVRRFAARVVWKLAPDAPLPPQTHTLLGVTFPGSGGCYLVDVGFGGQTPTSPLRLETGAVQPTTHEPYRLEDRV
DGFVLQAMVRDTWQTLYEFTTQTRPQIDLKVASWYASTHPASKFVTGLTAAVITDDARWNLSGRDLAVHRAGGTEKIRLA
DAAAVVDTLSERFGINVADIGERGALETRIDELLARQPGADAP
>Mature_282_residues
ALDLTAYFDRINYRGATDPTLDVLQDLVTVHSRTIPFENLDPLLGVPVDDLSPQALADKLVLRRRGGYCFEHNGLMGYVL
AELGYRVRRFAARVVWKLAPDAPLPPQTHTLLGVTFPGSGGCYLVDVGFGGQTPTSPLRLETGAVQPTTHEPYRLEDRVD
GFVLQAMVRDTWQTLYEFTTQTRPQIDLKVASWYASTHPASKFVTGLTAAVITDDARWNLSGRDLAVHRAGGTEKIRLAD
AAAVVDTLSERFGINVADIGERGALETRIDELLARQPGADAP

Specific function: Could have a role in acetylating, and hence inactivating, the antitubercular drug isoniazid

COG id: COG2162

COG function: function code Q; Arylamine N-acetyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the arylamine N-acetyltransferase family

Homologues:

Organism=Homo sapiens, GI237649132, Length=278, Percent_Identity=26.2589928057554, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI237649130, Length=278, Percent_Identity=26.2589928057554, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI237649128, Length=278, Percent_Identity=26.2589928057554, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI237649126, Length=278, Percent_Identity=26.2589928057554, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI237649124, Length=278, Percent_Identity=26.2589928057554, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI237649122, Length=278, Percent_Identity=26.2589928057554, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI237649119, Length=278, Percent_Identity=26.2589928057554, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI42741671, Length=278, Percent_Identity=26.2589928057554, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI237649009, Length=278, Percent_Identity=26.2589928057554, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI116295260, Length=265, Percent_Identity=26.0377358490566, Blast_Score=86, Evalue=5e-17,
Organism=Escherichia coli, GI1787736, Length=259, Percent_Identity=39.3822393822394, Blast_Score=156, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NAT_MYCBO (P0A5L9)

Other databases:

- EMBL:   BX248346
- RefSeq:   NP_857235.1
- ProteinModelPortal:   P0A5L9
- EnsemblBacteria:   EBMYCT00000014564
- GeneID:   1093447
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb3596c
- GeneTree:   EBGT00050000016624
- HOGENOM:   HBG544563
- OMA:   WYTSTHP
- ProtClustDB:   CLSK800002
- BioCyc:   MBOV233413:MB3596C-MONOMER
- BRENDA:   2.3.1.5
- GO:   GO:0005737
- InterPro:   IPR001447
- PANTHER:   PTHR11786

Pfam domain/function: PF00797 Acetyltransf_2

EC number: =2.3.1.5

Molecular weight: Translated: 31030; Mature: 30898

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: ACT_SITE 70-70 ACT_SITE 110-110 ACT_SITE 127-127

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALDLTAYFDRINYRGATDPTLDVLQDLVTVHSRTIPFENLDPLLGVPVDDLSPQALADK
CEEEHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHH
LVLRRRGGYCFEHNGLMGYVLAELGYRVRRFAARVVWKLAPDAPLPPQTHTLLGVTFPGS
HHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCC
GGCYLVDVGFGGQTPTSPLRLETGAVQPTTHEPYRLEDRVDGFVLQAMVRDTWQTLYEFT
CCEEEEEECCCCCCCCCCEEEECCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHH
TQTRPQIDLKVASWYASTHPASKFVTGLTAAVITDDARWNLSGRDLAVHRAGGTEKIRLA
HCCCCCEEEEEEEEECCCCCHHHHHHHHHHEEEECCCEECCCCCEEEEEECCCCCEEEHH
DAAAVVDTLSERFGINVADIGERGALETRIDELLARQPGADAP
HHHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
ALDLTAYFDRINYRGATDPTLDVLQDLVTVHSRTIPFENLDPLLGVPVDDLSPQALADK
EEEHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHH
LVLRRRGGYCFEHNGLMGYVLAELGYRVRRFAARVVWKLAPDAPLPPQTHTLLGVTFPGS
HHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCC
GGCYLVDVGFGGQTPTSPLRLETGAVQPTTHEPYRLEDRVDGFVLQAMVRDTWQTLYEFT
CCEEEEEECCCCCCCCCCEEEECCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHH
TQTRPQIDLKVASWYASTHPASKFVTGLTAAVITDDARWNLSGRDLAVHRAGGTEKIRLA
HCCCCCEEEEEEEEECCCCCHHHHHHHHHHEEEECCCEECCCCCEEEEEECCCCCEEEHH
DAAAVVDTLSERFGINVADIGERGALETRIDELLARQPGADAP
HHHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972