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The map label for this gene is bphI [C]

Identifier: 148663397

GI number: 148663397

Start: 3981218

End: 3982258

Strand: Reverse

Name: bphI [C]

Synonym: MRA_3573

Alternate gene names: 148663397

Gene position: 3982258-3981218 (Counterclockwise)

Preceding gene: 148663398

Following gene: 148663396

Centisome position: 90.1

GC content: 66.38

Gene sequence:

>1041_bases
ATGACCGATATGTGGGACGTCCGCATCACGGACACCTCGCTGCGGGATGGGTCGCACCACAAACGCCACCAATTCACCAA
GGACGAGGTCGGTGCCATCGTGGCCGCCCTAGACGCCGCTGGCGTGCCGGTGATCGAGGTGACCCACGGCGACGGCCTGG
GCGGCTCATCGTTCAACTATGGCTTCTCGAAAACCCCTGAGCAGGAGCTGATCAAGCTGGCCGCGGCGACCGCGAAAGAG
GCCCGGATCGCGTTTTTGATGTTGCCCGGTGTGGGCACCAAGGACGACATCAAAGAGGCGCGGGACAACGGCGGGTCGAT
CTGCCGGATCGCCACCCACTGCACCGAGGCCGACGTGTCGATCCAGCATTTCGGGCTGGCCCGCGAACTGGGTCTGGAAA
CCGTCGGGTTTTTGATGATGGCCCACACCATTGCGCCGGAGAAACTGGCCGCCCAGGCCCGCATCATGGCCGACGCCGGC
TGCCAGTGTGTCTACGTCGTCGACTCCGCCGGTGCCCTGGTGCTCGACGGCGTGGCCGACCGGGTGTCGGCCCTGGTCGC
CGAGCTCGGCGAAGACGCCCAGGTGGGCTTTCATGGGCACGAGAACCTCGGTCTCGGGGTGGCCAATTCGGTGGCGGCGG
TGCGCGCGGGCGCCAAGCAGATCGACGGGTCCTGCCGGCGTTTCGGCGCCGGGGCGGGTAACGCGCCGGTCGAGGCGCTG
ATCGGGGTGTTCGACAAGATCGGCGTCAAGACCGGCATTGATTTCTTCGACATTGCCGACGCCGCCGAGGACGTGGTGCG
CCCGGCCATGCCGGCCGAATGTCTGCTCGACCGCAACGCGTTGATCATGGGGTATTCCGGGGTCTACTCCAGCTTCCTCA
AGCATGCGGTCCGCCAGGCCGAGCGCTACGGCGTGCCGGCGTCGGCGCTGTTGCACCGGGCCGGTCAGCGCAAGCTCATC
GGTGGCCAGGAAGACCAGCTCATCGACATCGCGCTGGAAATCAAACGCGAGCTGGATAGCGGTGCGGCCGTAACGCATTG
A

Upstream 100 bases:

>100_bases
ACCTGCCGCCCTATGCGGGCAACCTGGACATCATGACCGCGGCGGCCACCAAGGTGGGCGAGGAGATCGCCAAGGAGACG
CTGGTTGTAGGAGGAGCGCG

Downstream 100 bases:

>100_bases
CGACACCGCTGGTTCGCGGATTTCACGGAAACGCAGATGGTCCGACCCGGTTTGTCCTACGGTTCTGACTGATTGTCAGG
TCGGGAAGGGACGGGGTCAT

Product: 4-hydroxy-2-ketovalerate aldolase

Products: NA

Alternate protein names: HOA 1; 4-hydroxy-2-keto-pentanoic acid aldolase 1; 4-hydroxy-2-oxopentanoate aldolase 1 [H]

Number of amino acids: Translated: 346; Mature: 345

Protein sequence:

>346_residues
MTDMWDVRITDTSLRDGSHHKRHQFTKDEVGAIVAALDAAGVPVIEVTHGDGLGGSSFNYGFSKTPEQELIKLAAATAKE
ARIAFLMLPGVGTKDDIKEARDNGGSICRIATHCTEADVSIQHFGLARELGLETVGFLMMAHTIAPEKLAAQARIMADAG
CQCVYVVDSAGALVLDGVADRVSALVAELGEDAQVGFHGHENLGLGVANSVAAVRAGAKQIDGSCRRFGAGAGNAPVEAL
IGVFDKIGVKTGIDFFDIADAAEDVVRPAMPAECLLDRNALIMGYSGVYSSFLKHAVRQAERYGVPASALLHRAGQRKLI
GGQEDQLIDIALEIKRELDSGAAVTH

Sequences:

>Translated_346_residues
MTDMWDVRITDTSLRDGSHHKRHQFTKDEVGAIVAALDAAGVPVIEVTHGDGLGGSSFNYGFSKTPEQELIKLAAATAKE
ARIAFLMLPGVGTKDDIKEARDNGGSICRIATHCTEADVSIQHFGLARELGLETVGFLMMAHTIAPEKLAAQARIMADAG
CQCVYVVDSAGALVLDGVADRVSALVAELGEDAQVGFHGHENLGLGVANSVAAVRAGAKQIDGSCRRFGAGAGNAPVEAL
IGVFDKIGVKTGIDFFDIADAAEDVVRPAMPAECLLDRNALIMGYSGVYSSFLKHAVRQAERYGVPASALLHRAGQRKLI
GGQEDQLIDIALEIKRELDSGAAVTH
>Mature_345_residues
TDMWDVRITDTSLRDGSHHKRHQFTKDEVGAIVAALDAAGVPVIEVTHGDGLGGSSFNYGFSKTPEQELIKLAAATAKEA
RIAFLMLPGVGTKDDIKEARDNGGSICRIATHCTEADVSIQHFGLARELGLETVGFLMMAHTIAPEKLAAQARIMADAGC
QCVYVVDSAGALVLDGVADRVSALVAELGEDAQVGFHGHENLGLGVANSVAAVRAGAKQIDGSCRRFGAGAGNAPVEALI
GVFDKIGVKTGIDFFDIADAAEDVVRPAMPAECLLDRNALIMGYSGVYSSFLKHAVRQAERYGVPASALLHRAGQRKLIG
GQEDQLIDIALEIKRELDSGAAVTH

Specific function: Catalyzes the retro-aldol cleavage of 4-hydroxy-2- oxopentanoate to pyruvate and acetaldehyde. Is involved in the meta-cleavage pathway for the degradation of aromatic compounds [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 4-hydroxy-2-oxovalerate aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1786548, Length=327, Percent_Identity=51.9877675840979, Blast_Score=328, Evalue=4e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017629
- InterPro:   IPR013785
- InterPro:   IPR012425
- InterPro:   IPR000891
- ProDom:   PD005364 [H]

Pfam domain/function: PF07836 DmpG_comm; PF00682 HMGL-like [H]

EC number: =4.1.3.39 [H]

Molecular weight: Translated: 36443; Mature: 36312

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDMWDVRITDTSLRDGSHHKRHQFTKDEVGAIVAALDAAGVPVIEVTHGDGLGGSSFNY
CCCCEEEEEECCCCCCCCCHHHHCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCC
GFSKTPEQELIKLAAATAKEARIAFLMLPGVGTKDDIKEARDNGGSICRIATHCTEADVS
CCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHCCCCCEEEEEECCCCCCCC
IQHFGLARELGLETVGFLMMAHTIAPEKLAAQARIMADAGCQCVYVVDSAGALVLDGVAD
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEEECCCCCEEEHHHHH
RVSALVAELGEDAQVGFHGHENLGLGVANSVAAVRAGAKQIDGSCRRFGAGAGNAPVEAL
HHHHHHHHCCCCCEECCCCCCCCCCCHHHHHHHHHHCHHHHCCHHHHHCCCCCCCHHHHH
IGVFDKIGVKTGIDFFDIADAAEDVVRPAMPAECLLDRNALIMGYSGVYSSFLKHAVRQA
HHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHCCCEEEEECHHHHHHHHHHHHHHH
ERYGVPASALLHRAGQRKLIGGQEDQLIDIALEIKRELDSGAAVTH
HHCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TDMWDVRITDTSLRDGSHHKRHQFTKDEVGAIVAALDAAGVPVIEVTHGDGLGGSSFNY
CCCEEEEEECCCCCCCCCHHHHCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCC
GFSKTPEQELIKLAAATAKEARIAFLMLPGVGTKDDIKEARDNGGSICRIATHCTEADVS
CCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHCCCCCEEEEEECCCCCCCC
IQHFGLARELGLETVGFLMMAHTIAPEKLAAQARIMADAGCQCVYVVDSAGALVLDGVAD
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEEECCCCCEEEHHHHH
RVSALVAELGEDAQVGFHGHENLGLGVANSVAAVRAGAKQIDGSCRRFGAGAGNAPVEAL
HHHHHHHHCCCCCEECCCCCCCCCCCHHHHHHHHHHCHHHHCCHHHHHCCCCCCCHHHHH
IGVFDKIGVKTGIDFFDIADAAEDVVRPAMPAECLLDRNALIMGYSGVYSSFLKHAVRQA
HHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHCCCEEEEECHHHHHHHHHHHHHHH
ERYGVPASALLHRAGQRKLIGGQEDQLIDIALEIKRELDSGAAVTH
HHCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA