Click here to switch to the map view.

The map label for this gene is narK2

Identifier: 148661536

GI number: 148661536

Start: 1965701

End: 1966888

Strand: Reverse

Name: narK2

Synonym: MRA_1748

Alternate gene names: 148661536

Gene position: 1966888-1965701 (Counterclockwise)

Preceding gene: 148661538

Following gene: 148661535

Centisome position: 44.5

GC content: 70.2

Gene sequence:

>1188_bases
ATGAGAGGGCAAGCGGCCAATCTCGTGCTGGCCACCTGGATCTCGGTGGTCAACTTCTGGGCGTGGAACCTGATCGGCCC
GCTGTCGACCAGCTACGCGCGTGACATGTCACTGTCCAGCGCCGAGGCGTCGCTGCTCGTCGCCACCCCGATCCTGGTGG
GTGCCCTTGGCCGCATCGTCACCGGGCCGCTCACCGACCGCTTCGGCGGGCGCGCCATGCTCATCGCGGTGACGCTGGCG
TCGATCCTCCCGGTGCTCGCGGTCGGGGTCGCGGCAACCATGGGCTCCTACGCGTTGCTGGTGTTTTTCGGGCTCTTCCT
GGGCGTTGCCGGCACGATCTTCGCCGTCGGCATCCCGTTCGCCAACAACTGGTACCAGCCGGCGCGGCGCGGTTTCTCCA
CCGGCGTGTTCGGTATGGGCATGGTCGGCACCGCGCTCTCGGCGTTCTTCACCCCGCGGTTTGTACGGTGGTTCGGCCTG
TTCACCACCCACGCCATCGTCGCGGCCGCGCTCGCGTCGACCGCCGTGGTGGCCATGGTCGTGCTTCGTGATGCACCCTA
CTTTCGGCCCAACGCCGACCCGGTGCTGCCCAGGCTCAAGGCCGCGGCACGGTTGCCGGTGACCTGGGAGATGTCGTTTC
TGTACGCGATCGTGTTCGGCGGGTTCGTGGCGTTCAGCAACTACCTGCCCACCTACATCACCACGATCTACGGGTTCTCC
ACGGTCGACGCGGGCGCTCGCACCGCCGGGTTCGCCCTGGCGGCGGTGCTGGCCCGGCCGGTGGGCGGGTGGCTCTCCGA
CCGGATCGCACCGAGGCACGTGGTGCTGGCCTCGCTCGCCGGGACCGCGCTGCTGGCGTTCGCCGCGGCGTTGCAGCCGC
CGCCGGAGGTGTGGTCGGCGGCCACCTTCATCACCCTGGCGGTCTGCCTCGGCGTGGGCACCGGCGGCGTGTTCGCGTGG
GTGGCCCGCCGCGCCCCGGCCGCATCGGTCGGCTCGGTCACCGGAATCGTCGCCGCGGCAGGCGGATTGGGCGGTTACTT
CCCGCCGCTGGTGATGGGCGCGACCTACGACCCGGTCGACAACGACTACACGGTCGGGTTGCTGCTGCTGGTGGCGACCG
CGCTGGTCGCGTGTACCTACACCGCGCTGCACGCGCGGGAGCCGGTGAGTGAGGAGGCGTCCAGGTGA

Upstream 100 bases:

>100_bases
CCGGCCCTAACTGAGCCGGCGCCATCGAAGCCAGGGTTAGCGCATGGCTGTCCGGGCGGGGCGCGGAGTTCGCCGGAGTC
CGAGACCCCGGATCGTGTCG

Downstream 100 bases:

>100_bases
CGGTGACACCACGGACCGGCAGCCGCATCGAGGAGCTGCTTGCACGCAGCGGCCGGTTCTTCATCCCGGGTGAGATCTCG
GCGGATCTGCGTACCGTGAC

Product: putative nitrate/nitrite transporter NarK2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MRGQAANLVLATWISVVNFWAWNLIGPLSTSYARDMSLSSAEASLLVATPILVGALGRIVTGPLTDRFGGRAMLIAVTLA
SILPVLAVGVAATMGSYALLVFFGLFLGVAGTIFAVGIPFANNWYQPARRGFSTGVFGMGMVGTALSAFFTPRFVRWFGL
FTTHAIVAAALASTAVVAMVVLRDAPYFRPNADPVLPRLKAAARLPVTWEMSFLYAIVFGGFVAFSNYLPTYITTIYGFS
TVDAGARTAGFALAAVLARPVGGWLSDRIAPRHVVLASLAGTALLAFAAALQPPPEVWSAATFITLAVCLGVGTGGVFAW
VARRAPAASVGSVTGIVAAAGGLGGYFPPLVMGATYDPVDNDYTVGLLLLVATALVACTYTALHAREPVSEEASR

Sequences:

>Translated_395_residues
MRGQAANLVLATWISVVNFWAWNLIGPLSTSYARDMSLSSAEASLLVATPILVGALGRIVTGPLTDRFGGRAMLIAVTLA
SILPVLAVGVAATMGSYALLVFFGLFLGVAGTIFAVGIPFANNWYQPARRGFSTGVFGMGMVGTALSAFFTPRFVRWFGL
FTTHAIVAAALASTAVVAMVVLRDAPYFRPNADPVLPRLKAAARLPVTWEMSFLYAIVFGGFVAFSNYLPTYITTIYGFS
TVDAGARTAGFALAAVLARPVGGWLSDRIAPRHVVLASLAGTALLAFAAALQPPPEVWSAATFITLAVCLGVGTGGVFAW
VARRAPAASVGSVTGIVAAAGGLGGYFPPLVMGATYDPVDNDYTVGLLLLVATALVACTYTALHAREPVSEEASR
>Mature_395_residues
MRGQAANLVLATWISVVNFWAWNLIGPLSTSYARDMSLSSAEASLLVATPILVGALGRIVTGPLTDRFGGRAMLIAVTLA
SILPVLAVGVAATMGSYALLVFFGLFLGVAGTIFAVGIPFANNWYQPARRGFSTGVFGMGMVGTALSAFFTPRFVRWFGL
FTTHAIVAAALASTAVVAMVVLRDAPYFRPNADPVLPRLKAAARLPVTWEMSFLYAIVFGGFVAFSNYLPTYITTIYGFS
TVDAGARTAGFALAAVLARPVGGWLSDRIAPRHVVLASLAGTALLAFAAALQPPPEVWSAATFITLAVCLGVGTGGVFAW
VARRAPAASVGSVTGIVAAAGGLGGYFPPLVMGATYDPVDNDYTVGLLLLVATALVACTYTALHAREPVSEEASR

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NARK2_MYCTU (P71995)

Other databases:

- EMBL:   AE000516
- EMBL:   BX842577
- PIR:   D70688
- RefSeq:   NP_216253.1
- RefSeq:   NP_336238.1
- ProteinModelPortal:   P71995
- EnsemblBacteria:   EBMYCT00000000253
- EnsemblBacteria:   EBMYCT00000071755
- GeneID:   885231
- GeneID:   923926
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT1779
- KEGG:   mtu:Rv1737c
- TIGR:   MT1779
- TubercuList:   Rv1737c
- GeneTree:   EBGT00050000014777
- HOGENOM:   HBG479449
- OMA:   GIVWVSL
- ProtClustDB:   CLSK871960
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196

Pfam domain/function: PF07690 MFS_1; SSF103473 MFS_gen_substrate_transporter

EC number: NA

Molecular weight: Translated: 41109; Mature: 41109

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1bfec994)-; HASH(0x1ed93690)-; HASH(0x1eca15f0)-; HASH(0x1e97e90c)-; HASH(0x1eaaaf9c)-; HASH(0x1b9c706c)-; HASH(0x1e5126e8)-; HASH(0x1ed195b4)-; HASH(0x1ecd74e4)-; HASH(0x1ec59db4)-; HASH(0x1ecd06c4)-; HASH(0x1eb180d4)-;

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCH
>Mature Secondary Structure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CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036