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The map label for this gene is ychM [C]

Identifier: 148661538

GI number: 148661538

Start: 1967473

End: 1969155

Strand: Reverse

Name: ychM [C]

Synonym: MRA_1750

Alternate gene names: 148661538

Gene position: 1969155-1967473 (Counterclockwise)

Preceding gene: 148661543

Following gene: 148661536

Centisome position: 44.55

GC content: 62.86

Gene sequence:

>1683_bases
ATGATTCCCACGATGACATCGGCCGGCTGGGCACCAGGGGTGGTGCAGTTCCGCGAATACCAACGGCGTTGGCTGCGCGG
CGATGTCCTCGCCGGCCTGACCGTGGCCGCCTATCTGATCCCGCAAGCGATGGCGTATGCGACCGTGGCGGGCCTACCGC
CGGCAGCCGGGCTGTGGGCGTCGATCGCGCCGCTTGCCATTTACGCACTGCTCGGATCGTCCCGGCAGCTTTCAATCGGC
CCGGAATCCGCCACCGCCTTGATGACGGCGGCCGTGCTCGCTCCGATGGCCGCCGGGGATCTTCGACGCTATGCCGTTCT
GGCGGCAACCCTCGGATTGCTAGTCGGCCTTATCTGCCTACTCGCTGGCACGGCGCGACTAGGTTTCCTCGCCAGCCTGC
GATCGCGGCCGGTGCTCGTCGGATACATGGCCGGCATCGCGCTTGTCATGATCTCCAGCCAACTCGGCACTATCACCGGC
ACCTCGGTCGAAGGCAACGAATTCTTCAGCGAAGTACACTCTTTCGCGACTAGCGTCACGCGAGTTCACTGGCCGACTTT
TGTGTTAGCCATGTCTGTCCTAGCGCTGCTAACTATGCTCACGCGGTGGGCGCCGCGCGCCCCCGGACCGATCATCGCGG
TTCTTGCGGCCACGATGCTAGTGGCCGTTATGTCCTTGGATGCCAAAGGTATTGCGATTGTGGGTCGGATACCTTCCGGT
CTGCCGACGCCGGGTGTGCCGCCCGTTTCGGTGGAAGACTTGCGGGCACTGATCATTCCGGCTGCCGGGATCGCGATTGT
TACCTTCACCGACGGTGTGTTGACCGCACGCGCCTTCGCCGCTCGTCGAGGTCAGGAAGTCAATGCCAACGCCGAGCTGC
GCGCGGTCGGGGCCTGCAACATCGCCGCCGGGCTGACACACGGTTTTCCGGTGAGTTCCAGCAGCAGCCGTACCGCCCTC
GCCGACGTCGTCGGTGGCCGCACCCAGCTGTACTCGCTGATCGCGTTGGGGCTTGTTGTCATCGTGATGGTTTTCGCGAG
TGGGCTGCTGGCCATGTTTCCGATCGCCGCTCTGGGCGCTTTGGTGGTATATGCCGCGCTACGCTTGATCGACTTGTCAG
AATTCCGGCGACTGGCGCGGTTTCGGCGCAGCGAACTCATGCTGGCACTAGCCACCACAGCAGCCGTGTTAGGCCTAGGA
GTGTTCTATGGAGTCCTCGCCGCGGTTGCCCTGTCCATCCTCGAACTGCTTCGTCGGGTCGCACATCCGCATGACAGCGT
TCTCGGGTTCGTGCCGGGCATTGCCGGCATGCACGACATCGATGACTATCCGCAGGCCAAGCGCGTGCCCGGGCTGGTGG
TGTATCGCTATGACGCGCCGTTGTGCTTCGCCAATGCCGAAGACTTCCGCAGGCGAGCACTGACCGTGGTCGATCAGGAT
CCGGGGCAAGTCGAGTGGTTCGTACTCAACGCCGAATCCAATGTGGAGGTCGACCTGACTGCGCTGGATGCGCTCGACCA
ACTCCGCACCGAGCTGCTGCGTCGGGGAATAGTGTTCGCCATGGCCCGGGTCAAACAAGACTTGCGTGAATCACTCAGGG
CCGCCAGTCTTCTCGATAAGATTGGCGAAGACCATATCTTTATGACATTGCCTACCGCAGTGCAGGCGTTCCGTCGGCGC
TAA

Upstream 100 bases:

>100_bases
CAAAGTCTCGCCCATTCGAGCCGCCAATTCCATGGTCAGGATTCTATAAAGATTGGGCGGGTTCTAGACCGACTCTAGCT
AAGCGGCCAGCCGTCGGACA

Downstream 100 bases:

>100_bases
CGCGCCGGCACCCTCAATACAACAATCGCGCCGGCTGATGGGTAACAGCTTCGATGTCGTGGGTCGACACCTTCAACATT
CGCTTCCCCAAGTCGGACAA

Product: sulfate transporter

Products: Proton [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 560; Mature: 560

Protein sequence:

>560_residues
MIPTMTSAGWAPGVVQFREYQRRWLRGDVLAGLTVAAYLIPQAMAYATVAGLPPAAGLWASIAPLAIYALLGSSRQLSIG
PESATALMTAAVLAPMAAGDLRRYAVLAATLGLLVGLICLLAGTARLGFLASLRSRPVLVGYMAGIALVMISSQLGTITG
TSVEGNEFFSEVHSFATSVTRVHWPTFVLAMSVLALLTMLTRWAPRAPGPIIAVLAATMLVAVMSLDAKGIAIVGRIPSG
LPTPGVPPVSVEDLRALIIPAAGIAIVTFTDGVLTARAFAARRGQEVNANAELRAVGACNIAAGLTHGFPVSSSSSRTAL
ADVVGGRTQLYSLIALGLVVIVMVFASGLLAMFPIAALGALVVYAALRLIDLSEFRRLARFRRSELMLALATTAAVLGLG
VFYGVLAAVALSILELLRRVAHPHDSVLGFVPGIAGMHDIDDYPQAKRVPGLVVYRYDAPLCFANAEDFRRRALTVVDQD
PGQVEWFVLNAESNVEVDLTALDALDQLRTELLRRGIVFAMARVKQDLRESLRAASLLDKIGEDHIFMTLPTAVQAFRRR

Sequences:

>Translated_560_residues
MIPTMTSAGWAPGVVQFREYQRRWLRGDVLAGLTVAAYLIPQAMAYATVAGLPPAAGLWASIAPLAIYALLGSSRQLSIG
PESATALMTAAVLAPMAAGDLRRYAVLAATLGLLVGLICLLAGTARLGFLASLRSRPVLVGYMAGIALVMISSQLGTITG
TSVEGNEFFSEVHSFATSVTRVHWPTFVLAMSVLALLTMLTRWAPRAPGPIIAVLAATMLVAVMSLDAKGIAIVGRIPSG
LPTPGVPPVSVEDLRALIIPAAGIAIVTFTDGVLTARAFAARRGQEVNANAELRAVGACNIAAGLTHGFPVSSSSSRTAL
ADVVGGRTQLYSLIALGLVVIVMVFASGLLAMFPIAALGALVVYAALRLIDLSEFRRLARFRRSELMLALATTAAVLGLG
VFYGVLAAVALSILELLRRVAHPHDSVLGFVPGIAGMHDIDDYPQAKRVPGLVVYRYDAPLCFANAEDFRRRALTVVDQD
PGQVEWFVLNAESNVEVDLTALDALDQLRTELLRRGIVFAMARVKQDLRESLRAASLLDKIGEDHIFMTLPTAVQAFRRR
>Mature_560_residues
MIPTMTSAGWAPGVVQFREYQRRWLRGDVLAGLTVAAYLIPQAMAYATVAGLPPAAGLWASIAPLAIYALLGSSRQLSIG
PESATALMTAAVLAPMAAGDLRRYAVLAATLGLLVGLICLLAGTARLGFLASLRSRPVLVGYMAGIALVMISSQLGTITG
TSVEGNEFFSEVHSFATSVTRVHWPTFVLAMSVLALLTMLTRWAPRAPGPIIAVLAATMLVAVMSLDAKGIAIVGRIPSG
LPTPGVPPVSVEDLRALIIPAAGIAIVTFTDGVLTARAFAARRGQEVNANAELRAVGACNIAAGLTHGFPVSSSSSRTAL
ADVVGGRTQLYSLIALGLVVIVMVFASGLLAMFPIAALGALVVYAALRLIDLSEFRRLARFRRSELMLALATTAAVLGLG
VFYGVLAAVALSILELLRRVAHPHDSVLGFVPGIAGMHDIDDYPQAKRVPGLVVYRYDAPLCFANAEDFRRRALTVVDQD
PGQVEWFVLNAESNVEVDLTALDALDQLRTELLRRGIVFAMARVKQDLRESLRAASLLDKIGEDHIFMTLPTAVQAFRRR

Specific function: Expression in E.coli induces sulfate uptake during early-to mid-log phase growth. Uptake is maximal at pH 6.0, is sulfate-specific, requires E.coli CysA and the transmembrane segment but not the STAS domain of the protein

COG id: COG0659

COG function: function code P; Sulfate permease and related transporters (MFS superfamily)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 STAS domain

Homologues:

Organism=Homo sapiens, GI45827800, Length=492, Percent_Identity=29.8780487804878, Blast_Score=196, Evalue=6e-50,
Organism=Homo sapiens, GI39752683, Length=492, Percent_Identity=29.8780487804878, Blast_Score=196, Evalue=7e-50,
Organism=Homo sapiens, GI94721253, Length=477, Percent_Identity=33.5429769392034, Blast_Score=189, Evalue=4e-48,
Organism=Homo sapiens, GI94721259, Length=477, Percent_Identity=33.5429769392034, Blast_Score=189, Evalue=4e-48,
Organism=Homo sapiens, GI94721255, Length=477, Percent_Identity=33.5429769392034, Blast_Score=189, Evalue=5e-48,
Organism=Homo sapiens, GI94721257, Length=479, Percent_Identity=33.4029227557411, Blast_Score=189, Evalue=5e-48,
Organism=Homo sapiens, GI269784651, Length=487, Percent_Identity=28.3367556468172, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI45827802, Length=449, Percent_Identity=29.3986636971047, Blast_Score=161, Evalue=2e-39,
Organism=Homo sapiens, GI4557535, Length=513, Percent_Identity=26.7056530214425, Blast_Score=157, Evalue=4e-38,
Organism=Homo sapiens, GI262206105, Length=586, Percent_Identity=27.8156996587031, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI262206075, Length=586, Percent_Identity=27.8156996587031, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI262206069, Length=586, Percent_Identity=27.8156996587031, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI262206063, Length=586, Percent_Identity=27.8156996587031, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI4505697, Length=500, Percent_Identity=25.6, Blast_Score=146, Evalue=6e-35,
Organism=Homo sapiens, GI47131207, Length=517, Percent_Identity=28.8201160541586, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI20336272, Length=517, Percent_Identity=28.8201160541586, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI100913030, Length=620, Percent_Identity=23.3870967741935, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI16418413, Length=545, Percent_Identity=25.1376146788991, Blast_Score=132, Evalue=7e-31,
Organism=Homo sapiens, GI217272867, Length=545, Percent_Identity=25.3211009174312, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI16418457, Length=493, Percent_Identity=22.3123732251521, Blast_Score=128, Evalue=2e-29,
Organism=Homo sapiens, GI301601599, Length=493, Percent_Identity=22.3123732251521, Blast_Score=128, Evalue=2e-29,
Organism=Homo sapiens, GI20336282, Length=510, Percent_Identity=22.156862745098, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI16306483, Length=510, Percent_Identity=22.156862745098, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI65506789, Length=378, Percent_Identity=28.5714285714286, Blast_Score=91, Evalue=4e-18,
Organism=Homo sapiens, GI45827804, Length=263, Percent_Identity=27.3764258555133, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI301601602, Length=291, Percent_Identity=22.680412371134, Blast_Score=80, Evalue=8e-15,
Organism=Escherichia coli, GI87081859, Length=512, Percent_Identity=23.6328125, Blast_Score=67, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17551690, Length=582, Percent_Identity=25.2577319587629, Blast_Score=159, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI17566848, Length=556, Percent_Identity=25.5395683453237, Blast_Score=158, Evalue=6e-39,
Organism=Caenorhabditis elegans, GI17562578, Length=633, Percent_Identity=23.5387045813586, Blast_Score=153, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI86564196, Length=631, Percent_Identity=23.9302694136292, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI193203292, Length=486, Percent_Identity=26.9547325102881, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI86565215, Length=557, Percent_Identity=24.5960502692998, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI86564876, Length=648, Percent_Identity=22.2222222222222, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI86565213, Length=246, Percent_Identity=25.2032520325203, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI86565209, Length=240, Percent_Identity=23.3333333333333, Blast_Score=70, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI86565211, Length=240, Percent_Identity=23.3333333333333, Blast_Score=69, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6325260, Length=584, Percent_Identity=22.6027397260274, Blast_Score=128, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6319771, Length=470, Percent_Identity=26.1702127659574, Blast_Score=124, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6323121, Length=479, Percent_Identity=27.1398747390397, Blast_Score=120, Evalue=7e-28,
Organism=Drosophila melanogaster, GI24649801, Length=457, Percent_Identity=27.3522975929978, Blast_Score=167, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24666186, Length=529, Percent_Identity=25.8979206049149, Blast_Score=159, Evalue=5e-39,
Organism=Drosophila melanogaster, GI24651449, Length=461, Percent_Identity=27.5488069414317, Blast_Score=151, Evalue=1e-36,
Organism=Drosophila melanogaster, GI19922482, Length=567, Percent_Identity=24.5149911816578, Blast_Score=151, Evalue=1e-36,
Organism=Drosophila melanogaster, GI21358229, Length=457, Percent_Identity=26.6958424507659, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI85815873, Length=440, Percent_Identity=27.2727272727273, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI21355087, Length=440, Percent_Identity=25.9090909090909, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24647160, Length=440, Percent_Identity=25.9090909090909, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24663084, Length=544, Percent_Identity=25.9191176470588, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI21357695, Length=544, Percent_Identity=25.9191176470588, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI21358633, Length=531, Percent_Identity=22.7871939736347, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y1739_MYCTU (P71997)

Other databases:

- EMBL:   BX842577
- EMBL:   AE000516
- PIR:   F70688
- RefSeq:   NP_216255.1
- RefSeq:   NP_336240.1
- ProteinModelPortal:   P71997
- EnsemblBacteria:   EBMYCT00000002700
- EnsemblBacteria:   EBMYCT00000071445
- GeneID:   887208
- GeneID:   923915
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT1781
- KEGG:   mtu:Rv1739c
- TIGR:   MT1781
- TubercuList:   Rv1739c
- GeneTree:   EBGT00050000016006
- HOGENOM:   HBG554309
- OMA:   YACILPM
- ProtClustDB:   CLSK871961
- InterPro:   IPR002645
- InterPro:   IPR001902
- InterPro:   IPR011547
- Gene3D:   G3DSA:3.30.750.24
- TIGRFAMs:   TIGR00815

Pfam domain/function: PF01740 STAS; PF00916 Sulfate_transp; SSF52091 STAS

EC number: NA

Molecular weight: Translated: 59368; Mature: 59368

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: PS00211 ABC_TRANSPORTER_1; PS50801 STAS

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1e61af00)-; HASH(0x1b245dbc)-; HASH(0x1e91f630)-; HASH(0x1ec12d0c)-; HASH(0x1edead08)-; HASH(0x1e997d28)-; HASH(0x1def4dc4)-; HASH(0x1ebcf1c8)-; HASH(0x1f139058)-; HASH(0x1e6753f4)-; HASH(0x1e2e3af4)-;

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPTMTSAGWAPGVVQFREYQRRWLRGDVLAGLTVAAYLIPQAMAYATVAGLPPAAGLWA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
SIAPLAIYALLGSSRQLSIGPESATALMTAAVLAPMAAGDLRRYAVLAATLGLLVGLICL
HHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAGTARLGFLASLRSRPVLVGYMAGIALVMISSQLGTITGTSVEGNEFFSEVHSFATSVT
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHHHHH
RVHWPTFVLAMSVLALLTMLTRWAPRAPGPIIAVLAATMLVAVMSLDAKGIAIVGRIPSG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCC
LPTPGVPPVSVEDLRALIIPAAGIAIVTFTDGVLTARAFAARRGQEVNANAELRAVGACN
CCCCCCCCCCHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHCCCCCCCCCCEEEEECCH
IAAGLTHGFPVSSSSSRTALADVVGGRTQLYSLIALGLVVIVMVFASGLLAMFPIAALGA
HHHHCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVVYAALRLIDLSEFRRLARFRRSELMLALATTAAVLGLGVFYGVLAAVALSILELLRRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AHPHDSVLGFVPGIAGMHDIDDYPQAKRVPGLVVYRYDAPLCFANAEDFRRRALTVVDQD
CCCCHHHHHHHCCCCCCCCCCCCCCHHCCCCEEEEEECCCEEECCHHHHHHHHHEEEECC
PGQVEWFVLNAESNVEVDLTALDALDQLRTELLRRGIVFAMARVKQDLRESLRAASLLDK
CCCEEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGEDHIFMTLPTAVQAFRRR
HCCCCEEEECHHHHHHHHCH
>Mature Secondary Structure
MIPTMTSAGWAPGVVQFREYQRRWLRGDVLAGLTVAAYLIPQAMAYATVAGLPPAAGLWA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
SIAPLAIYALLGSSRQLSIGPESATALMTAAVLAPMAAGDLRRYAVLAATLGLLVGLICL
HHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAGTARLGFLASLRSRPVLVGYMAGIALVMISSQLGTITGTSVEGNEFFSEVHSFATSVT
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHHHHH
RVHWPTFVLAMSVLALLTMLTRWAPRAPGPIIAVLAATMLVAVMSLDAKGIAIVGRIPSG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCC
LPTPGVPPVSVEDLRALIIPAAGIAIVTFTDGVLTARAFAARRGQEVNANAELRAVGACN
CCCCCCCCCCHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHCCCCCCCCCCEEEEECCH
IAAGLTHGFPVSSSSSRTALADVVGGRTQLYSLIALGLVVIVMVFASGLLAMFPIAALGA
HHHHCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVVYAALRLIDLSEFRRLARFRRSELMLALATTAAVLGLGVFYGVLAAVALSILELLRRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AHPHDSVLGFVPGIAGMHDIDDYPQAKRVPGLVVYRYDAPLCFANAEDFRRRALTVVDQD
CCCCHHHHHHHCCCCCCCCCCCCCCHHCCCCEEEEEECCCEEECCHHHHHHHHHEEEECC
PGQVEWFVLNAESNVEVDLTALDALDQLRTELLRRGIVFAMARVKQDLRESLRAASLLDK
CCCEEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGEDHIFMTLPTAVQAFRRR
HCCCCEEEECHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; SO42- [Periplasm] [C]

Specific reaction: Proton [Periplasm] + SO42- [Periplasm] = Proton [Cytoplasm] + SO42- [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036