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The map label for this gene is narX

Identifier: 148661535

GI number: 148661535

Start: 1963746

End: 1965704

Strand: Reverse

Name: narX

Synonym: MRA_1747

Alternate gene names: 148661535

Gene position: 1965704-1963746 (Counterclockwise)

Preceding gene: 148661536

Following gene: 148661534

Centisome position: 44.47

GC content: 68.15

Gene sequence:

>1959_bases
GTGACGGTGACACCACGGACCGGCAGCCGCATCGAGGAGCTGCTTGCACGCAGCGGCCGGTTCTTCATCCCGGGTGAGAT
CTCGGCGGATCTGCGTACCGTGACCCGCCGCGGCGGCCGCGACGGCGACGTGTTCTATCGAGACCGGTGGAGCCACGACA
AGGTGGTCCGCTCCACACACGGGGTGAATTGCACCGGGTCGTGTTCTTGGAAGATCTACGTCAAAGACGACATCATCACC
TGGGAGACGCAGGAGACCGACTATCCGTCGGTGGGCCCGGACCGGCCCGAGTATGAGCCCCGCGGCTGCCCGCGCGGCGC
GGCGTTTTCCTGGTACACGTATTCGCCGACGCGGGTGCGCCATCCGTACGCCCGCGGCGTGCTTGTCGAGATGTATCGGG
AGGCGAAGGCACGTTTGGGTGATCCGGTGGCGGCCTGGGCCGACATCCAGGCCGACCCGCGGCGGCGCCGCCGCTACCAG
CGCGCCCGCGGCAAGGGCGGGCTGGTCCGGGTCAGCTGGGCCGAGGCCACCGAGATGATCGCCGCCGCCCACGTGCACAC
CATCTCCACATACGGCCCGGACCGGGTTGCCGGCTTCTCCCCCATCCCGGCGATGTCCATGGTGAGCCACGCCGCGGGGT
CGCGGTTCGTGGAGCTAATCGGCGGGGTGATGACGTCGTTCTACGACTGGTACGCCGACCTGCCGGTGGCCTCCCCGCAG
GTGTTCGGCGACCAGACCGACGTGCCGGAGTCCGGAGATTGGTGGGACGTGGTGTGGCAATGCGCCTCGGTGCTGCTGAC
CTACCCGAACTCACGGCAACTCGGCACCGCAGAGGAATTGCTGGCCCACATCGACGGTCCGGCCGCGGATCTGTTGGGGC
GCACGGTCTCTGAGCTGCGCCGTGCCGATCCGCTGACCGCGGCGACCCGCTACGTCGACACCTTCGACCTGCGAGGCCGC
GCCACCCTGTACCTGACCTACTGGACCGCCGGCGACACCCGCAACCGCGGCCGGGAGATGCTGGCCTTCGCCCAGACCTA
CCGCAGCACCGACGTCGCACCACCGCGCGGCGAGACCCCGGACTTCCTGCCGGTGGTGCTCGAATTCGCCGCGACCGTCG
ACCCCGAGGCGGGGCGACGGTTGCTGAGCGGGTACCGGGTGCCCATCGCCGCGCTGTGCAATGCCCTGACCGAGGCCGCA
TTGCCATACGCACACACGGTGGCCGCGGTATGCCGGACGGGTGACATGATGGGCGAACTCTTCTGGACCGTCGTGCCGTA
TGTGACGATGACGATCGTCGCGGTCGGCTCCTGGTGGCGCTACCGCTATGACAAATTCGGCTGGACCACCCGCTCGTCCC
AGCTGTACGAGTCGCGGCTGCTGCGGATCGCCAGCCCGATGTTTCATTTCGGCATCCTGGTGGTCATCGTCGGCCACGGT
ATCGGGCTCGTGATCCCGCAGTCGTGGACTCAGGCCGCCGGTTTGAGCGAGGGCGCATATCACGTGCAGGCCGTCGTGCT
GGGGTCGATCGCCGGCATCACCACCTTGGCCGGCGTTACCCTGCTGATCTACCGGCGGCGCACCCGCGGGCCGGTGTTCA
TGGCTACCACCGTCAACGACAAGGTGATGTACCTCGTGCTGGTGGCGGCGATCGTCGCGGGACTGGGTGCGACGGCGTTG
GGCTCCGGCGTTGTCGGCGAGGCGTACAACTACCGCGAGACGGTGTCGGTGTGGTTCCGCTCGGTGTGGGTACTGCAACC
GCGCGGGGACCTGATGGCCGAGGCTCCGCTGTATTACCAGATCCATGTGCTGATCGGGTTGGCGTTGTTCGCGTTGTGGC
CGTTCACCCGGCTGGTACACGCGTTCAGCGCCCCGATCGGCTATCTGTTCCGCCCGTACATCATCTACCGCAGCCGCGAG
GAGCTGGTGCTAACGCGGCCGCGGCGGCGCGGGTGGTGA

Upstream 100 bases:

>100_bases
ACTACACGGTCGGGTTGCTGCTGCTGGTGGCGACCGCGCTGGTCGCGTGTACCTACACCGCGCTGCACGCGCGGGAGCCG
GTGAGTGAGGAGGCGTCCAG

Downstream 100 bases:

>100_bases
CGGGAGTGCGTCTTCGGCGAGCGGACAGGACTGCCCATCGAGATCGACGTTAGCGACACGTCTACTCGTCGAACAAGTCC
GCGCTGGCGTCGATTTCGAT

Product: nitrate reductase NarX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 652; Mature: 651

Protein sequence:

>652_residues
MTVTPRTGSRIEELLARSGRFFIPGEISADLRTVTRRGGRDGDVFYRDRWSHDKVVRSTHGVNCTGSCSWKIYVKDDIIT
WETQETDYPSVGPDRPEYEPRGCPRGAAFSWYTYSPTRVRHPYARGVLVEMYREAKARLGDPVAAWADIQADPRRRRRYQ
RARGKGGLVRVSWAEATEMIAAAHVHTISTYGPDRVAGFSPIPAMSMVSHAAGSRFVELIGGVMTSFYDWYADLPVASPQ
VFGDQTDVPESGDWWDVVWQCASVLLTYPNSRQLGTAEELLAHIDGPAADLLGRTVSELRRADPLTAATRYVDTFDLRGR
ATLYLTYWTAGDTRNRGREMLAFAQTYRSTDVAPPRGETPDFLPVVLEFAATVDPEAGRRLLSGYRVPIAALCNALTEAA
LPYAHTVAAVCRTGDMMGELFWTVVPYVTMTIVAVGSWWRYRYDKFGWTTRSSQLYESRLLRIASPMFHFGILVVIVGHG
IGLVIPQSWTQAAGLSEGAYHVQAVVLGSIAGITTLAGVTLLIYRRRTRGPVFMATTVNDKVMYLVLVAAIVAGLGATAL
GSGVVGEAYNYRETVSVWFRSVWVLQPRGDLMAEAPLYYQIHVLIGLALFALWPFTRLVHAFSAPIGYLFRPYIIYRSRE
ELVLTRPRRRGW

Sequences:

>Translated_652_residues
MTVTPRTGSRIEELLARSGRFFIPGEISADLRTVTRRGGRDGDVFYRDRWSHDKVVRSTHGVNCTGSCSWKIYVKDDIIT
WETQETDYPSVGPDRPEYEPRGCPRGAAFSWYTYSPTRVRHPYARGVLVEMYREAKARLGDPVAAWADIQADPRRRRRYQ
RARGKGGLVRVSWAEATEMIAAAHVHTISTYGPDRVAGFSPIPAMSMVSHAAGSRFVELIGGVMTSFYDWYADLPVASPQ
VFGDQTDVPESGDWWDVVWQCASVLLTYPNSRQLGTAEELLAHIDGPAADLLGRTVSELRRADPLTAATRYVDTFDLRGR
ATLYLTYWTAGDTRNRGREMLAFAQTYRSTDVAPPRGETPDFLPVVLEFAATVDPEAGRRLLSGYRVPIAALCNALTEAA
LPYAHTVAAVCRTGDMMGELFWTVVPYVTMTIVAVGSWWRYRYDKFGWTTRSSQLYESRLLRIASPMFHFGILVVIVGHG
IGLVIPQSWTQAAGLSEGAYHVQAVVLGSIAGITTLAGVTLLIYRRRTRGPVFMATTVNDKVMYLVLVAAIVAGLGATAL
GSGVVGEAYNYRETVSVWFRSVWVLQPRGDLMAEAPLYYQIHVLIGLALFALWPFTRLVHAFSAPIGYLFRPYIIYRSRE
ELVLTRPRRRGW
>Mature_651_residues
TVTPRTGSRIEELLARSGRFFIPGEISADLRTVTRRGGRDGDVFYRDRWSHDKVVRSTHGVNCTGSCSWKIYVKDDIITW
ETQETDYPSVGPDRPEYEPRGCPRGAAFSWYTYSPTRVRHPYARGVLVEMYREAKARLGDPVAAWADIQADPRRRRRYQR
ARGKGGLVRVSWAEATEMIAAAHVHTISTYGPDRVAGFSPIPAMSMVSHAAGSRFVELIGGVMTSFYDWYADLPVASPQV
FGDQTDVPESGDWWDVVWQCASVLLTYPNSRQLGTAEELLAHIDGPAADLLGRTVSELRRADPLTAATRYVDTFDLRGRA
TLYLTYWTAGDTRNRGREMLAFAQTYRSTDVAPPRGETPDFLPVVLEFAATVDPEAGRRLLSGYRVPIAALCNALTEAAL
PYAHTVAAVCRTGDMMGELFWTVVPYVTMTIVAVGSWWRYRYDKFGWTTRSSQLYESRLLRIASPMFHFGILVVIVGHGI
GLVIPQSWTQAAGLSEGAYHVQAVVLGSIAGITTLAGVTLLIYRRRTRGPVFMATTVNDKVMYLVLVAAIVAGLGATALG
SGVVGEAYNYRETVSVWFRSVWVLQPRGDLMAEAPLYYQIHVLIGLALFALWPFTRLVHAFSAPIGYLFRPYIIYRSREE
LVLTRPRRRGW

Specific function: Does not seem to have nitrate reductase activity

COG id: COG5013

COG function: function code C; Nitrate reductase alpha subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the nitrate reductase gamma subunit family

Homologues:

Organism=Escherichia coli, GI1787477, Length=246, Percent_Identity=59.349593495935, Blast_Score=318, Evalue=1e-87,
Organism=Escherichia coli, GI1787741, Length=246, Percent_Identity=57.7235772357724, Blast_Score=309, Evalue=4e-85,
Organism=Escherichia coli, GI1787738, Length=227, Percent_Identity=33.4801762114537, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1787480, Length=227, Percent_Identity=29.5154185022026, Blast_Score=103, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NARX_MYCTU (P71994)

Other databases:

- EMBL:   AE000516
- EMBL:   BX842577
- PIR:   C70688
- RefSeq:   NP_216252.1
- RefSeq:   NP_336237.1
- HSSP:   P11350
- ProteinModelPortal:   P71994
- EnsemblBacteria:   EBMYCT00000001283
- EnsemblBacteria:   EBMYCT00000069411
- GeneID:   885213
- GeneID:   923930
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT1778
- KEGG:   mtu:Rv1736c
- TIGR:   MT1778
- TubercuList:   Rv1736c
- GeneTree:   EBGT00070000032259
- HOGENOM:   HBG565178
- ProtClustDB:   CLSK791361
- GO:   GO:0006810
- InterPro:   IPR020945
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- InterPro:   IPR003816
- InterPro:   IPR003765
- SMART:   SM00926
- TIGRFAMs:   TIGR00351
- TIGRFAMs:   TIGR00684

Pfam domain/function: PF00384 Molybdopterin; PF02613 Nitrate_red_del; PF02665 Nitrate_red_gam

EC number: =1.7.99.4

Molecular weight: Translated: 72826; Mature: 72695

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: PS00551 MOLYBDOPTERIN_PROK_1; PS00932 MOLYBDOPTERIN_PROK_3

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1b2d50ac)-; HASH(0x1e61ba8c)-; HASH(0x1ec4f308)-; HASH(0x1ec73d6c)-; HASH(0x1d8f902c)-;

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVTPRTGSRIEELLARSGRFFIPGEISADLRTVTRRGGRDGDVFYRDRWSHDKVVRSTH
CCCCCCCCHHHHHHHHHCCCEEECCCCCHHHHHHHHCCCCCCCEEECCCCCCHHHHHHCC
GVNCTGSCSWKIYVKDDIITWETQETDYPSVGPDRPEYEPRGCPRGAAFSWYTYSPTRVR
CCCCCCCCEEEEEEECCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCC
HPYARGVLVEMYREAKARLGDPVAAWADIQADPRRRRRYQRARGKGGLVRVSWAEATEMI
CCHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHCCCCCEEEEEHHHHHHHH
AAAHVHTISTYGPDRVAGFSPIPAMSMVSHAAGSRFVELIGGVMTSFYDWYADLPVASPQ
HHHHHHEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
VFGDQTDVPESGDWWDVVWQCASVLLTYPNSRQLGTAEELLAHIDGPAADLLGRTVSELR
HCCCCCCCCCCCCHHHHHHHHHHHHEECCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHH
RADPLTAATRYVDTFDLRGRATLYLTYWTAGDTRNRGREMLAFAQTYRSTDVAPPRGETP
HCCCHHHHHHHHHCEECCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
DFLPVVLEFAATVDPEAGRRLLSGYRVPIAALCNALTEAALPYAHTVAAVCRTGDMMGEL
CHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
FWTVVPYVTMTIVAVGSWWRYRYDKFGWTTRSSQLYESRLLRIASPMFHFGILVVIVGHG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
IGLVIPQSWTQAAGLSEGAYHVQAVVLGSIAGITTLAGVTLLIYRRRTRGPVFMATTVND
CEEEECCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCC
KVMYLVLVAAIVAGLGATALGSGVVGEAYNYRETVSVWFRSVWVLQPRGDLMAEAPLYYQ
HHHHHHHHHHHHHCCCHHHHCCCCCCCCCCHHHHHHHHHHHHEEECCCCCCEECCCHHHH
IHVLIGLALFALWPFTRLVHAFSAPIGYLFRPYIIYRSREELVLTRPRRRGW
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCEEEEECCCCEEEECCCCCCH
>Mature Secondary Structure 
TVTPRTGSRIEELLARSGRFFIPGEISADLRTVTRRGGRDGDVFYRDRWSHDKVVRSTH
CCCCCCCHHHHHHHHHCCCEEECCCCCHHHHHHHHCCCCCCCEEECCCCCCHHHHHHCC
GVNCTGSCSWKIYVKDDIITWETQETDYPSVGPDRPEYEPRGCPRGAAFSWYTYSPTRVR
CCCCCCCCEEEEEEECCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCC
HPYARGVLVEMYREAKARLGDPVAAWADIQADPRRRRRYQRARGKGGLVRVSWAEATEMI
CCHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHCCCCCEEEEEHHHHHHHH
AAAHVHTISTYGPDRVAGFSPIPAMSMVSHAAGSRFVELIGGVMTSFYDWYADLPVASPQ
HHHHHHEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
VFGDQTDVPESGDWWDVVWQCASVLLTYPNSRQLGTAEELLAHIDGPAADLLGRTVSELR
HCCCCCCCCCCCCHHHHHHHHHHHHEECCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHH
RADPLTAATRYVDTFDLRGRATLYLTYWTAGDTRNRGREMLAFAQTYRSTDVAPPRGETP
HCCCHHHHHHHHHCEECCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
DFLPVVLEFAATVDPEAGRRLLSGYRVPIAALCNALTEAALPYAHTVAAVCRTGDMMGEL
CHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
FWTVVPYVTMTIVAVGSWWRYRYDKFGWTTRSSQLYESRLLRIASPMFHFGILVVIVGHG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
IGLVIPQSWTQAAGLSEGAYHVQAVVLGSIAGITTLAGVTLLIYRRRTRGPVFMATTVND
CEEEECCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCC
KVMYLVLVAAIVAGLGATALGSGVVGEAYNYRETVSVWFRSVWVLQPRGDLMAEAPLYYQ
HHHHHHHHHHHHHCCCHHHHCCCCCCCCCCHHHHHHHHHHHHEEECCCCCCEECCCHHHH
IHVLIGLALFALWPFTRLVHAFSAPIGYLFRPYIIYRSREELVLTRPRRRGW
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCEEEEECCCCEEEECCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036