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The map label for this gene is glgB [H]

Identifier: 148661115

GI number: 148661115

Start: 1491427

End: 1493622

Strand: Reverse

Name: glgB [H]

Synonym: MRA_1334

Alternate gene names: 148661115

Gene position: 1493622-1491427 (Counterclockwise)

Preceding gene: 148661116

Following gene: 148661114

Centisome position: 33.79

GC content: 64.03

Gene sequence:

>2196_bases
ATGAGTCGATCCGAGAAACTCACCGGGGAGCACCTTGCACCCGAGCCGGCCGAAATGGCGCGCTTGGTGGCGGGTACACA
TCACAACCCGCACGGCATCCTGGGCGCCCACGAATACGACGACCATACCGTCATCCGAGCGTTCCGTCCGCATGCCGTCG
AGGTCGTCGCGCTCGTTGGTAAGGACCGGTTCTCGTTGCAGCACCTCGATTCTGGCCTGTTTGCCGTCGCATTGCCGTTC
GTCGACCTCATCGACTACCGCCTGCAGGTGACCTATGAAGGTTGCGAGCCACACACCGTGGCCGATGCGTACCGATTCCT
GCCCACCCTGGGCGAGGTCGACCTGCACCTGTTCGCCGAGGGCCGCCACGAACGGCTTTGGGAAGTCCTGGGTGCCCACC
CCCGCTCGTTTACCACGGCCGACGGTGTGGTGAGTGGCGTGTCGTTCGCCGTGTGGGCGCCCAACGCCAAGGGCGTCAGC
TTGATCGGCGAGTTCAACGGTTGGAATGGCCACGAAGCCCCCATGCGGGTGCTCGGCCCATCAGGGGTATGGGAATTGTT
CTGGCCCGACTTCCCTTGCGACGGTCTGTACAAGTTCCGCGTGCACGGCGCCGACGGCGTGGTTACCGATCGGGCCGACC
CGTTCGCGTTCGGCACCGAGGTGCCGCCGCAGACCGCATCGCGGGTGACGTCGAGTGACTACACCTGGGGTGACGACGAC
TGGATGGCTGGGCGTGCGCTGCGCAACCCGGTGAACGAGGCGATGAGCACCTACGAAGTCCATCTCGGTTCGTGGCGGCC
TGGACTCAGCTACCGCCAGCTTGCTCGTGAGTTGACGGATTACATTGTGGATCAAGGGTTTACCCATGTGGAGCTGTTGC
CCGTCGCCGAGCATCCATTCGCCGGATCATGGGGGTATCAGGTCACGTCCTACTATGCGCCGACATCACGATTCGGCACA
CCCGACGACTTCCGGGCGCTGGTCGACGCCCTGCACCAGGCCGGCATCGGCGTCATCGTGGATTGGGTCCCAGCGCACTT
CCCGAAGGACGCGTGGGCCCTGGGACGGTTCGACGGCACTCCGCTCTACGAACATTCCGATCCCAAACGCGGCGAGCAAC
TGGATTGGGGCACATACGTGTTCGACTTCGGCCGCCCGGAAGTGCGCAACTTTCTGGTAGCCAATGCGTTGTACTGGCTA
CAGGAGTTCCACATCGACGGCCTGCGGGTGGACGCGGTGGCCTCAATGCTCTATCTAGACTACTCGCGACCCGAGGGCGG
CTGGACCCCCAACGTCCACGGCGGCCGGGAGAACCTGGAAGCAGTGCAGTTCCTGCAGGAGATGAACGCCACGGCGCACA
AGGTCGCGCCGGGAATCGTCACCATCGCCGAGGAGTCCACGCCGTGGTCTGGGGTGACCCGCCCGACCAACATTGGCGGC
CTGGGCTTTTCGATGAAGTGGAACATGGGCTGGATGCACGACACGCTCGACTACGTCAGCCGAGATCCGGTGTACCGCAG
CTACCACCACCACGAGATGACGTTCTCGATGCTGTATGCGTTCAGCGAAAATTACGTGTTGCCGCTCAGTCATGACGAGG
TGGTGCACGGCAAAGGCACGCTGTGGGGGCGGATGCCGGGCAACAATCACGTCAAGGCCGCCGGCCTGCGTAGCCTGCTT
GCCTACCAATGGGCACACCCCGGCAAGCAATTGCTGTTCATGGGTCAGGAATTCGGCCAACGCGCCGAATGGTCCGAGCA
GCGCGGCCTGGACTGGTTCCAACTCGACGAAAACGGCTTCTCCAACGGGATTCAGCGGCTGGTGCGCGACATCAACGACA
TCTACCGATGCCACCCGGCGCTGTGGAGCTTAGACACCACCCCCGAAGGCTATTCTTGGATCGACGCCAACGACTCCGCC
AACAATGTGTTGAGCTTTATGCGCTACGGCAGCGACGGCTCGGTGCTGGCCTGCGTGTTCAATTTCGCAGGTGCCGAACA
CCGTGACTATCGACTCGGGCTGCCGCGCGCGGGCCGCTGGCGCGAGGTGCTCAATACCGACGCGACGATCTACCACGGCT
CAGGGATCGGCAACCTCGGCGGCGTGGACGCCACCGACGACCCCTGGCATGGCCGCCCGGCGTCCGCGGTGCTGGTGCTG
CCGCCCACTTCGGCGCTGTGGCTGACGCCCGCCTAG

Upstream 100 bases:

>100_bases
ATCCGCATCGACCCAGCACGGGCAGTCGCCCACATCATCAACATGCCAGCCGTGCCCTACGAGAGCCGAAACACGCTGCT
GCGCAGGAGGTGACGGGCTC

Downstream 100 bases:

>100_bases
CGCCTGCCATGAGCAGATTGCACATGCCTGATGAGCCGATTCGATGCACTCTGCACACTGCACTCCGAGATTGATCAGAA
AGGCTAAGCGATAGCGCTAC

Product: glycogen branching enzyme

Products: NA

Alternate protein names: 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase; Glycogen-branching enzyme; BE [H]

Number of amino acids: Translated: 731; Mature: 730

Protein sequence:

>731_residues
MSRSEKLTGEHLAPEPAEMARLVAGTHHNPHGILGAHEYDDHTVIRAFRPHAVEVVALVGKDRFSLQHLDSGLFAVALPF
VDLIDYRLQVTYEGCEPHTVADAYRFLPTLGEVDLHLFAEGRHERLWEVLGAHPRSFTTADGVVSGVSFAVWAPNAKGVS
LIGEFNGWNGHEAPMRVLGPSGVWELFWPDFPCDGLYKFRVHGADGVVTDRADPFAFGTEVPPQTASRVTSSDYTWGDDD
WMAGRALRNPVNEAMSTYEVHLGSWRPGLSYRQLARELTDYIVDQGFTHVELLPVAEHPFAGSWGYQVTSYYAPTSRFGT
PDDFRALVDALHQAGIGVIVDWVPAHFPKDAWALGRFDGTPLYEHSDPKRGEQLDWGTYVFDFGRPEVRNFLVANALYWL
QEFHIDGLRVDAVASMLYLDYSRPEGGWTPNVHGGRENLEAVQFLQEMNATAHKVAPGIVTIAEESTPWSGVTRPTNIGG
LGFSMKWNMGWMHDTLDYVSRDPVYRSYHHHEMTFSMLYAFSENYVLPLSHDEVVHGKGTLWGRMPGNNHVKAAGLRSLL
AYQWAHPGKQLLFMGQEFGQRAEWSEQRGLDWFQLDENGFSNGIQRLVRDINDIYRCHPALWSLDTTPEGYSWIDANDSA
NNVLSFMRYGSDGSVLACVFNFAGAEHRDYRLGLPRAGRWREVLNTDATIYHGSGIGNLGGVDATDDPWHGRPASAVLVL
PPTSALWLTPA

Sequences:

>Translated_731_residues
MSRSEKLTGEHLAPEPAEMARLVAGTHHNPHGILGAHEYDDHTVIRAFRPHAVEVVALVGKDRFSLQHLDSGLFAVALPF
VDLIDYRLQVTYEGCEPHTVADAYRFLPTLGEVDLHLFAEGRHERLWEVLGAHPRSFTTADGVVSGVSFAVWAPNAKGVS
LIGEFNGWNGHEAPMRVLGPSGVWELFWPDFPCDGLYKFRVHGADGVVTDRADPFAFGTEVPPQTASRVTSSDYTWGDDD
WMAGRALRNPVNEAMSTYEVHLGSWRPGLSYRQLARELTDYIVDQGFTHVELLPVAEHPFAGSWGYQVTSYYAPTSRFGT
PDDFRALVDALHQAGIGVIVDWVPAHFPKDAWALGRFDGTPLYEHSDPKRGEQLDWGTYVFDFGRPEVRNFLVANALYWL
QEFHIDGLRVDAVASMLYLDYSRPEGGWTPNVHGGRENLEAVQFLQEMNATAHKVAPGIVTIAEESTPWSGVTRPTNIGG
LGFSMKWNMGWMHDTLDYVSRDPVYRSYHHHEMTFSMLYAFSENYVLPLSHDEVVHGKGTLWGRMPGNNHVKAAGLRSLL
AYQWAHPGKQLLFMGQEFGQRAEWSEQRGLDWFQLDENGFSNGIQRLVRDINDIYRCHPALWSLDTTPEGYSWIDANDSA
NNVLSFMRYGSDGSVLACVFNFAGAEHRDYRLGLPRAGRWREVLNTDATIYHGSGIGNLGGVDATDDPWHGRPASAVLVL
PPTSALWLTPA
>Mature_730_residues
SRSEKLTGEHLAPEPAEMARLVAGTHHNPHGILGAHEYDDHTVIRAFRPHAVEVVALVGKDRFSLQHLDSGLFAVALPFV
DLIDYRLQVTYEGCEPHTVADAYRFLPTLGEVDLHLFAEGRHERLWEVLGAHPRSFTTADGVVSGVSFAVWAPNAKGVSL
IGEFNGWNGHEAPMRVLGPSGVWELFWPDFPCDGLYKFRVHGADGVVTDRADPFAFGTEVPPQTASRVTSSDYTWGDDDW
MAGRALRNPVNEAMSTYEVHLGSWRPGLSYRQLARELTDYIVDQGFTHVELLPVAEHPFAGSWGYQVTSYYAPTSRFGTP
DDFRALVDALHQAGIGVIVDWVPAHFPKDAWALGRFDGTPLYEHSDPKRGEQLDWGTYVFDFGRPEVRNFLVANALYWLQ
EFHIDGLRVDAVASMLYLDYSRPEGGWTPNVHGGRENLEAVQFLQEMNATAHKVAPGIVTIAEESTPWSGVTRPTNIGGL
GFSMKWNMGWMHDTLDYVSRDPVYRSYHHHEMTFSMLYAFSENYVLPLSHDEVVHGKGTLWGRMPGNNHVKAAGLRSLLA
YQWAHPGKQLLFMGQEFGQRAEWSEQRGLDWFQLDENGFSNGIQRLVRDINDIYRCHPALWSLDTTPEGYSWIDANDSAN
NVLSFMRYGSDGSVLACVFNFAGAEHRDYRLGLPRAGRWREVLNTDATIYHGSGIGNLGGVDATDDPWHGRPASAVLVLP
PTSALWLTPA

Specific function: Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position [H]

COG id: COG0296

COG function: function code G; 1,4-alpha-glucan branching enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI189458812, Length=657, Percent_Identity=28.158295281583, Blast_Score=183, Evalue=4e-46,
Organism=Escherichia coli, GI1789839, Length=720, Percent_Identity=48.6111111111111, Blast_Score=661, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17554896, Length=635, Percent_Identity=25.9842519685039, Blast_Score=162, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI32564391, Length=513, Percent_Identity=26.7056530214425, Blast_Score=147, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6320826, Length=641, Percent_Identity=25.2730109204368, Blast_Score=159, Evalue=1e-39,
Organism=Drosophila melanogaster, GI28573410, Length=605, Percent_Identity=27.603305785124, Blast_Score=174, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006407
- InterPro:   IPR006048
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02806 Alpha-amylase_C; PF02922 CBM_48 [H]

EC number: =2.4.1.18 [H]

Molecular weight: Translated: 81730; Mature: 81599

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRSEKLTGEHLAPEPAEMARLVAGTHHNPHGILGAHEYDDHTVIRAFRPHAVEVVALVG
CCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEEEC
KDRFSLQHLDSGLFAVALPFVDLIDYRLQVTYEGCEPHTVADAYRFLPTLGEVDLHLFAE
CCCHHHHHHCCCHHHHHHHHHHHHCEEEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEEC
GRHERLWEVLGAHPRSFTTADGVVSGVSFAVWAPNAKGVSLIGEFNGWNGHEAPMRVLGP
CHHHHHHHHHCCCCCCCCCHHHHHCCCEEEEECCCCCCEEEEEECCCCCCCCCCEEEECC
SGVWELFWPDFPCDGLYKFRVHGADGVVTDRADPFAFGTEVPPQTASRVTSSDYTWGDDD
CCCEEEECCCCCCCCCEEEEEECCCCEEECCCCCCCCCCCCCCHHHHHCCCCCCCCCCCC
WMAGRALRNPVNEAMSTYEVHLGSWRPGLSYRQLARELTDYIVDQGFTHVELLPVAEHPF
HHHHHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCC
AGSWGYQVTSYYAPTSRFGTPDDFRALVDALHQAGIGVIVDWVPAHFPKDAWALGRFDGT
CCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHEECCCCCC
PLYEHSDPKRGEQLDWGTYVFDFGRPEVRNFLVANALYWLQEFHIDGLRVDAVASMLYLD
CCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHEE
YSRPEGGWTPNVHGGRENLEAVQFLQEMNATAHKVAPGIVTIAEESTPWSGVTRPTNIGG
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHCCCCEEEEECCCCCCCCCCCCCCCCC
LGFSMKWNMGWMHDTLDYVSRDPVYRSYHHHEMTFSMLYAFSENYVLPLSHDEVVHGKGT
CCEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEECCCE
LWGRMPGNNHVKAAGLRSLLAYQWAHPGKQLLFMGQEFGQRAEWSEQRGLDWFQLDENGF
EEEECCCCCCEEHHHHHHHHHHHHCCCCHHEEEECHHHCCCCCCHHHHCCCEEEECCCHH
SNGIQRLVRDINDIYRCHPALWSLDTTPEGYSWIDANDSANNVLSFMRYGSDGSVLACVF
HHHHHHHHHHHHHHHHHCHHHEECCCCCCCCEEEECCCCHHHHHHHHHCCCCCCEEEEEE
NFAGAEHRDYRLGLPRAGRWREVLNTDATIYHGSGIGNLGGVDATDDPWHGRPASAVLVL
HHCCCCCCCEEECCCCCCCHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEE
PPTSALWLTPA
CCCCCEEEECC
>Mature Secondary Structure 
SRSEKLTGEHLAPEPAEMARLVAGTHHNPHGILGAHEYDDHTVIRAFRPHAVEVVALVG
CCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEEEC
KDRFSLQHLDSGLFAVALPFVDLIDYRLQVTYEGCEPHTVADAYRFLPTLGEVDLHLFAE
CCCHHHHHHCCCHHHHHHHHHHHHCEEEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEEC
GRHERLWEVLGAHPRSFTTADGVVSGVSFAVWAPNAKGVSLIGEFNGWNGHEAPMRVLGP
CHHHHHHHHHCCCCCCCCCHHHHHCCCEEEEECCCCCCEEEEEECCCCCCCCCCEEEECC
SGVWELFWPDFPCDGLYKFRVHGADGVVTDRADPFAFGTEVPPQTASRVTSSDYTWGDDD
CCCEEEECCCCCCCCCEEEEEECCCCEEECCCCCCCCCCCCCCHHHHHCCCCCCCCCCCC
WMAGRALRNPVNEAMSTYEVHLGSWRPGLSYRQLARELTDYIVDQGFTHVELLPVAEHPF
HHHHHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCC
AGSWGYQVTSYYAPTSRFGTPDDFRALVDALHQAGIGVIVDWVPAHFPKDAWALGRFDGT
CCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHEECCCCCC
PLYEHSDPKRGEQLDWGTYVFDFGRPEVRNFLVANALYWLQEFHIDGLRVDAVASMLYLD
CCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHEE
YSRPEGGWTPNVHGGRENLEAVQFLQEMNATAHKVAPGIVTIAEESTPWSGVTRPTNIGG
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHCCCCEEEEECCCCCCCCCCCCCCCCC
LGFSMKWNMGWMHDTLDYVSRDPVYRSYHHHEMTFSMLYAFSENYVLPLSHDEVVHGKGT
CCEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEECCCE
LWGRMPGNNHVKAAGLRSLLAYQWAHPGKQLLFMGQEFGQRAEWSEQRGLDWFQLDENGF
EEEECCCCCCEEHHHHHHHHHHHHCCCCHHEEEECHHHCCCCCCHHHHCCCEEEECCCHH
SNGIQRLVRDINDIYRCHPALWSLDTTPEGYSWIDANDSANNVLSFMRYGSDGSVLACVF
HHHHHHHHHHHHHHHHHCHHHEECCCCCCCCEEEECCCCHHHHHHHHHCCCCCCEEEEEE
NFAGAEHRDYRLGLPRAGRWREVLNTDATIYHGSGIGNLGGVDATDDPWHGRPASAVLVL
HHCCCCCCCEEECCCCCCCHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEE
PPTSALWLTPA
CCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]