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The map label for this gene is glgE

Identifier: 148661116

GI number: 148661116

Start: 1493630

End: 1495735

Strand: Reverse

Name: glgE

Synonym: MRA_1335

Alternate gene names: 148661116

Gene position: 1495735-1493630 (Counterclockwise)

Preceding gene: 148661118

Following gene: 148661115

Centisome position: 33.84

GC content: 64.43

Gene sequence:

>2106_bases
GTGAGTGGCCGGGCAATCGGAACGGAAACGGAGTGGTGGGTGCCCGGTCGTGTCGAAATCGATGACGTCGCGCCCGTCGT
TTCGTGCGGCGTATATCCCGCCAAGGCGGTGGTCGGCGAGGTGGTCCCGGTCAGCGCGGCGGTCTGGCGTGAAGGCCACG
AGGCCGTCGCAGCGACGCTGGTCGTGCGCTACCTCGGAGTGCGTTACCCACACCTCACCGACAGACCCCGGGCCAGGGTG
CTTCCGACGCCGAGCGAGCCCCAACAACGCGTCAAGCCGCTGCTGATCCCGATGACGAGCGGCCAGGAGCCCTTCGTTTT
CCACGGCCAGTTCACCCCCGACCGGGTCGGATTGTGGACCTTCCGGGTGGATGGTTGGGGTGACCCGATCCACACCTGGC
GCCATGGGCTGATAGCCAAGCTAGATGCCGGCCAGGGAGAGACCGAGCTGTCCAACGACCTGTTGGTAGGCGCGGTGCTG
TTGGAGCGCGCGGCGACCGGTGTGCCGCGCGGGTTACGCGATCCCCTCCTGGCGGCCGCGGCAGCGCTGCGGACCCCCGG
TGACCCGGTGACCCGCACCGCGTTGGCCCTGACACCGGAAATCGAAGAGCTGCTGGCCGACTATCCGCTGCGGGACCTGG
TCACCCGGGGCGAGCAATTCGGCGTCTGGGTGGATCGGCCGTTGGCCCGGTTCGGCGCTTGGTATGAGATGTTTCCGCGC
TCAACCGGCGGGTGGGACGACGACGGCAACCCGGTACACGGCACCTTCGCCACCGCTGCGGCAGAACTTCCGCGCATCGC
CGGCATGGGGTTCGACGTGGTGTACCTGCCGCCGATCCATCCAATTGGCAAGGTGCATCGCAAGGGTCGCAACAACTCGC
CCACCGCCGCACCGACAGACGTGGGATCGCCGTGGGCGATCGGTAGCGATGAGGGCGGTCACGATACCGTTCATCCCAGC
CTGGGCACCATCGACGACTTCGACGACTTCGTCTCCGCGGCACGCGATCTGGGCATGGAGGTCGCGCTGGACCTGGCGCT
GCAATGCGCACCGGATCATCCGTGGGCCCGCGAACACCGGCAGTGGTTCACCGAGCTGCCGGACGGCACCATCGCCTACG
CGGAGAATCCACCGAAGAAGTACCAGGACATCTATCCGCTCAACTTCGACAACGATCCCGAGGGCCTGTACGACGAAGTG
CTGCGCGTGGTGCAACATTGGGTTAACCACGGCGTCAAGTTCTTTCGCGTCGACAATCCCCACACCAAACCACCCAACTT
CTGGGCCTGGCTGATCGCGCAGGTGAAGACCGTCGACCCCGACGTGCTGTTCCTGTCCGAGGCTTTCACCCCGCCCGCCC
GCCAGTACGGGCTGGCCAAGCTCGGCTTCACGCAGTCCTACAGCTATTTCACCTGGCGCACGACCAAGTGGGAGCTCACC
GAATTCGGCAACCAGATAGCCGAACTCGCCGACTACCGTCGGCCCAACCTGTTCGTCAACACCCCGGACATCCTGCACGC
GGTGCTGCAGCACAACGGTCCAGGCATGTTCGCCATCCGCGCGGTGCTGGCCGCCACCATGAGCCCAGCCTGGGGGATGT
ACTGCGGTTATGAGCTTTTCGAGCACCGTGCGGTGCGCGAGGGCAGCGAGGAGTACCTGGACTCGGAGAAGTACGAATTG
CGTCCCCGCGACTTTGCCAGCGCGCTGGACCAGGGTAGATCTTTGCAGCCGTTCATCACACGGCTCAATATAATTCGCCG
GCTGCACCCGGCGTTTCAACAGTTGCGTACCATTCATTTTCACCACGTTGACAACGACGCGTTGCTGGCCTACAGCAAGT
TCGACCCGGCCACCGGCGACTGCGTGTTGGTGGTGGTGACACTCAACGCATTTGGTCCTGAAGAAGCTACGCTGTGGTTG
GACATGGCGGCGTTGGGCATGGAGGACTACGACCGGTTTTGGGTGCGCGACGAGATAACCGGCGAAGAATACCAATGGGG
GCAAGCCAATTACATCCGCATCGACCCAGCACGGGCAGTCGCCCACATCATCAACATGCCAGCCGTGCCCTACGAGAGCC
GAAACACGCTGCTGCGCAGGAGGTGA

Upstream 100 bases:

>100_bases
CGCCCGCCGACCGCTGTGCCTTCGCGGGTGTGATCGGATACTAGGGTGGGTATCGGGCGCAAGAAGGACACACAAGGAAC
GACACATACAAGATGTCCCG

Downstream 100 bases:

>100_bases
CGGGCTCATGAGTCGATCCGAGAAACTCACCGGGGAGCACCTTGCACCCGAGCCGGCCGAAATGGCGCGCTTGGTGGCGG
GTACACATCACAACCCGCAC

Product: putative glucanase GlgE

Products: D-Glucose 6-phosphate; D-Glucose [C]

Alternate protein names: NA

Number of amino acids: Translated: 701; Mature: 700

Protein sequence:

>701_residues
MSGRAIGTETEWWVPGRVEIDDVAPVVSCGVYPAKAVVGEVVPVSAAVWREGHEAVAATLVVRYLGVRYPHLTDRPRARV
LPTPSEPQQRVKPLLIPMTSGQEPFVFHGQFTPDRVGLWTFRVDGWGDPIHTWRHGLIAKLDAGQGETELSNDLLVGAVL
LERAATGVPRGLRDPLLAAAAALRTPGDPVTRTALALTPEIEELLADYPLRDLVTRGEQFGVWVDRPLARFGAWYEMFPR
STGGWDDDGNPVHGTFATAAAELPRIAGMGFDVVYLPPIHPIGKVHRKGRNNSPTAAPTDVGSPWAIGSDEGGHDTVHPS
LGTIDDFDDFVSAARDLGMEVALDLALQCAPDHPWAREHRQWFTELPDGTIAYAENPPKKYQDIYPLNFDNDPEGLYDEV
LRVVQHWVNHGVKFFRVDNPHTKPPNFWAWLIAQVKTVDPDVLFLSEAFTPPARQYGLAKLGFTQSYSYFTWRTTKWELT
EFGNQIAELADYRRPNLFVNTPDILHAVLQHNGPGMFAIRAVLAATMSPAWGMYCGYELFEHRAVREGSEEYLDSEKYEL
RPRDFASALDQGRSLQPFITRLNIIRRLHPAFQQLRTIHFHHVDNDALLAYSKFDPATGDCVLVVVTLNAFGPEEATLWL
DMAALGMEDYDRFWVRDEITGEEYQWGQANYIRIDPARAVAHIINMPAVPYESRNTLLRRR

Sequences:

>Translated_701_residues
MSGRAIGTETEWWVPGRVEIDDVAPVVSCGVYPAKAVVGEVVPVSAAVWREGHEAVAATLVVRYLGVRYPHLTDRPRARV
LPTPSEPQQRVKPLLIPMTSGQEPFVFHGQFTPDRVGLWTFRVDGWGDPIHTWRHGLIAKLDAGQGETELSNDLLVGAVL
LERAATGVPRGLRDPLLAAAAALRTPGDPVTRTALALTPEIEELLADYPLRDLVTRGEQFGVWVDRPLARFGAWYEMFPR
STGGWDDDGNPVHGTFATAAAELPRIAGMGFDVVYLPPIHPIGKVHRKGRNNSPTAAPTDVGSPWAIGSDEGGHDTVHPS
LGTIDDFDDFVSAARDLGMEVALDLALQCAPDHPWAREHRQWFTELPDGTIAYAENPPKKYQDIYPLNFDNDPEGLYDEV
LRVVQHWVNHGVKFFRVDNPHTKPPNFWAWLIAQVKTVDPDVLFLSEAFTPPARQYGLAKLGFTQSYSYFTWRTTKWELT
EFGNQIAELADYRRPNLFVNTPDILHAVLQHNGPGMFAIRAVLAATMSPAWGMYCGYELFEHRAVREGSEEYLDSEKYEL
RPRDFASALDQGRSLQPFITRLNIIRRLHPAFQQLRTIHFHHVDNDALLAYSKFDPATGDCVLVVVTLNAFGPEEATLWL
DMAALGMEDYDRFWVRDEITGEEYQWGQANYIRIDPARAVAHIINMPAVPYESRNTLLRRR
>Mature_700_residues
SGRAIGTETEWWVPGRVEIDDVAPVVSCGVYPAKAVVGEVVPVSAAVWREGHEAVAATLVVRYLGVRYPHLTDRPRARVL
PTPSEPQQRVKPLLIPMTSGQEPFVFHGQFTPDRVGLWTFRVDGWGDPIHTWRHGLIAKLDAGQGETELSNDLLVGAVLL
ERAATGVPRGLRDPLLAAAAALRTPGDPVTRTALALTPEIEELLADYPLRDLVTRGEQFGVWVDRPLARFGAWYEMFPRS
TGGWDDDGNPVHGTFATAAAELPRIAGMGFDVVYLPPIHPIGKVHRKGRNNSPTAAPTDVGSPWAIGSDEGGHDTVHPSL
GTIDDFDDFVSAARDLGMEVALDLALQCAPDHPWAREHRQWFTELPDGTIAYAENPPKKYQDIYPLNFDNDPEGLYDEVL
RVVQHWVNHGVKFFRVDNPHTKPPNFWAWLIAQVKTVDPDVLFLSEAFTPPARQYGLAKLGFTQSYSYFTWRTTKWELTE
FGNQIAELADYRRPNLFVNTPDILHAVLQHNGPGMFAIRAVLAATMSPAWGMYCGYELFEHRAVREGSEEYLDSEKYELR
PRDFASALDQGRSLQPFITRLNIIRRLHPAFQQLRTIHFHHVDNDALLAYSKFDPATGDCVLVVVTLNAFGPEEATLWLD
MAALGMEDYDRFWVRDEITGEEYQWGQANYIRIDPARAVAHIINMPAVPYESRNTLLRRR

Specific function: Could be involved in glycogen catabolism

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family. GlgE subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLGE_MYCBO (P63532)

Other databases:

- EMBL:   BX248338
- RefSeq:   NP_855016.1
- ProteinModelPortal:   P63532
- EnsemblBacteria:   EBMYCT00000014933
- GeneID:   1090655
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb1362c
- GeneTree:   EBGT00050000016659
- HOGENOM:   HBG301516
- OMA:   MRIEPWH
- ProtClustDB:   CLSK2751738
- BioCyc:   MBOV233413:MB1362C-MONOMER
- InterPro:   IPR021828
- InterPro:   IPR006047
- InterPro:   IPR017853
- InterPro:   IPR013781
- Gene3D:   G3DSA:3.20.20.80

Pfam domain/function: PF00128 Alpha-amylase; PF11896 DUF3416; SSF51445 Glyco_hydro_cat

EC number: 3.2.1.93 [C]

Molecular weight: Translated: 78641; Mature: 78509

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: NA

Important sites: ACT_SITE 418-418 ACT_SITE 447-447

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGRAIGTETEWWVPGRVEIDDVAPVVSCGVYPAKAVVGEVVPVSAAVWREGHEAVAATL
CCCCCCCCCCCEECCCEEEHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHCCHHHHHHHH
VVRYLGVRYPHLTDRPRARVLPTPSEPQQRVKPLLIPMTSGQEPFVFHGQFTPDRVGLWT
HHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCCEEEEEECCCCCCEEEECCCCCCCEEEEE
FRVDGWGDPIHTWRHGLIAKLDAGQGETELSNDLLVGAVLLERAATGVPRGLRDPLLAAA
EEECCCCCHHHHHHCCCEEEECCCCCCCHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHH
AALRTPGDPVTRTALALTPEIEELLADYPLRDLVTRGEQFGVWVDRPLARFGAWYEMFPR
HHHCCCCCCHHHHHEEECHHHHHHHHCCCHHHHHHCCCCCCEECCCHHHHHHHHHHHCCC
STGGWDDDGNPVHGTFATAAAELPRIAGMGFDVVYLPPIHPIGKVHRKGRNNSPTAAPTD
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHCCCCCCCCCCCCC
VGSPWAIGSDEGGHDTVHPSLGTIDDFDDFVSAARDLGMEVALDLALQCAPDHPWAREHR
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHEEHHHEEECCCCCHHHHHH
QWFTELPDGTIAYAENPPKKYQDIYPLNFDNDPEGLYDEVLRVVQHWVNHGVKFFRVDNP
HHHHHCCCCEEEECCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCC
HTKPPNFWAWLIAQVKTVDPDVLFLSEAFTPPARQYGLAKLGFTQSYSYFTWRTTKWELT
CCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHCCHHCCCCCCCCEEEEECCEEEHH
EFGNQIAELADYRRPNLFVNTPDILHAVLQHNGPGMFAIRAVLAATMSPAWGMYCGYELF
HHHHHHHHHHHCCCCCEEECCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHH
EHRAVREGSEEYLDSEKYELRPRDFASALDQGRSLQPFITRLNIIRRLHPAFQQLRTIHF
HHHHHHCCCHHHHCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHE
HHVDNDALLAYSKFDPATGDCVLVVVTLNAFGPEEATLWLDMAALGMEDYDRFWVRDEIT
EECCCCEEEEEECCCCCCCCEEEEEEEECCCCCCCCEEEEHHHHHCCHHHHHEEEEECCC
GEEYQWGQANYIRIDPARAVAHIINMPAVPYESRNTLLRRR
CCCCCCCCCCEEEECHHHHHHHHHCCCCCCCCCCCHHHCCC
>Mature Secondary Structure 
SGRAIGTETEWWVPGRVEIDDVAPVVSCGVYPAKAVVGEVVPVSAAVWREGHEAVAATL
CCCCCCCCCCEECCCEEEHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHCCHHHHHHHH
VVRYLGVRYPHLTDRPRARVLPTPSEPQQRVKPLLIPMTSGQEPFVFHGQFTPDRVGLWT
HHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCCEEEEEECCCCCCEEEECCCCCCCEEEEE
FRVDGWGDPIHTWRHGLIAKLDAGQGETELSNDLLVGAVLLERAATGVPRGLRDPLLAAA
EEECCCCCHHHHHHCCCEEEECCCCCCCHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHH
AALRTPGDPVTRTALALTPEIEELLADYPLRDLVTRGEQFGVWVDRPLARFGAWYEMFPR
HHHCCCCCCHHHHHEEECHHHHHHHHCCCHHHHHHCCCCCCEECCCHHHHHHHHHHHCCC
STGGWDDDGNPVHGTFATAAAELPRIAGMGFDVVYLPPIHPIGKVHRKGRNNSPTAAPTD
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHCCCCCCCCCCCCC
VGSPWAIGSDEGGHDTVHPSLGTIDDFDDFVSAARDLGMEVALDLALQCAPDHPWAREHR
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHEEHHHEEECCCCCHHHHHH
QWFTELPDGTIAYAENPPKKYQDIYPLNFDNDPEGLYDEVLRVVQHWVNHGVKFFRVDNP
HHHHHCCCCEEEECCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCC
HTKPPNFWAWLIAQVKTVDPDVLFLSEAFTPPARQYGLAKLGFTQSYSYFTWRTTKWELT
CCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHCCHHCCCCCCCCEEEEECCEEEHH
EFGNQIAELADYRRPNLFVNTPDILHAVLQHNGPGMFAIRAVLAATMSPAWGMYCGYELF
HHHHHHHHHHHCCCCCEEECCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHH
EHRAVREGSEEYLDSEKYELRPRDFASALDQGRSLQPFITRLNIIRRLHPAFQQLRTIHF
HHHHHHCCCHHHHCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHE
HHVDNDALLAYSKFDPATGDCVLVVVTLNAFGPEEATLWLDMAALGMEDYDRFWVRDEIT
EECCCCEEEEEECCCCCCCCEEEEEEEECCCCCCCCEEEEHHHHHCCHHHHHEEEEECCC
GEEYQWGQANYIRIDPARAVAHIINMPAVPYESRNTLLRRR
CCCCCCCCCCEEEECHHHHHHHHHCCCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2O; alpha,alpha'-Trehalose 6-phosphate [C]

Specific reaction: H2O + alpha,alpha'-Trehalose 6-phosphate --> D-Glucose 6-phosphate + D-Glucose [C]

General reaction: O-Glycosyl bond hydrolysis [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972