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The map label for this gene is queA

Identifier: 148653721

GI number: 148653721

Start: 2391011

End: 2392183

Strand: Direct

Name: queA

Synonym: PsycPRwf_1924

Alternate gene names: 148653721

Gene position: 2391011-2392183 (Clockwise)

Preceding gene: 148653720

Following gene: 148653722

Centisome position: 80.26

GC content: 47.57

Gene sequence:

>1173_bases
ATGACTCAGCCTCTCTCTCAAGACCAACATGACAGCTCAAGCAATATGCCTACTGACAACGCCGAAACACAAGGCTATCT
CAGTGTTGCTGATTACGATTATGACCTGCCTGATGAGTATATCGCTCGTTATCCGCTGGCTGAGCGCAGTGCGTCACGCT
TGATGTATTTGCCAGCTCAAAAAGAAAATACGGCTGTCATACAAGACAGACAATTTTCTGACTTGCCTGAGCTATTACAG
GCAGGCGACTTGATAGTGTTTAACGACACTAAGGTGATGAAAGCCCGCTTATTTGGCCAAAAAGATACCGGCGGCAAGGT
AGAGGTTTTGGTCGAAAGACTTACCGATGACACTGAGTTTACTACCGATGAGAGCGGACAAAGCTTTGACAATGTGGCAC
TGTGTCATGTGCGTGCCAGCAAAGCGCCAAAGCTTGGGCAGCGCTTAGTTTTAGGGGATGGCAACATTGAATGCGTGATG
ATTGGCCGTCATGAGAACTTATTTATTTTAAGCTTTAAAAACCCTATCCTGCCAGACTTAGAGCAGTATGGCGAGTTGCC
AATCCCGCCGTATTTTGAACGTCATGCCGATGAAACTGACAATGTGCGTTATCAAACCGTATTTCATGACCCGACCAAGC
TTGCCAGCGTAGCTGCCCCCACCGCAAGCCTACACTTTGATGATAAAGTACTCAAGCAGCTGGCCGATAAAGGCATTAAC
ACCGCTTACGTCACCTTACATGTGGGCGCTGGCACCTTTGCACCGGTCAAGACCGACAACCTACTTAATCACACCATGCA
CAGCGAGTATGCCAATCTGCCGCAAGCCACCGCGGATCTCATTAATCAGACGCATCAAAACGGTAATAAAGTAATCGCTA
TTGGTACCACAGTGACTCGTGTGCTAGAGACCGCTTACCAAAAGACTGCCGACGAGCACGGCCATATCTCCACGTGGTCT
GGTGATACTGATATCTTTATCTACCCTGGCTTTAAATTTGGCGTGATTGATAAGCTGTTGACCAACTTTCACTTGCCCAA
ATCAACGTTACTCATGCTGGTTTCCGCTTTTGCCGGAAAAGCCAATATCGAGCAGGCTTATGAGCATGCGGTTGCCCAAA
AATACCGCTTCTTTAGCTATGGTGATGCCATGTTACTTGAACGTAACCTGTGA

Upstream 100 bases:

>100_bases
AACTCAAAAAACAGTTATTTCTGTTAAACTACTCCGCATTGCGCACAGCCTTGGCTTGCGGCACACCCCACTTTTATATT
GACCCGATTAAAGACAACTT

Downstream 100 bases:

>100_bases
TAAGCCGTCAGTCATAAAATAAGTCATTAGCATCAATAATAAGCATCAACACCTTAATTTACGCAAATAAAAGATTCTGA
ACGTTACAAATGGCTGGTGA

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 390; Mature: 389

Protein sequence:

>390_residues
MTQPLSQDQHDSSSNMPTDNAETQGYLSVADYDYDLPDEYIARYPLAERSASRLMYLPAQKENTAVIQDRQFSDLPELLQ
AGDLIVFNDTKVMKARLFGQKDTGGKVEVLVERLTDDTEFTTDESGQSFDNVALCHVRASKAPKLGQRLVLGDGNIECVM
IGRHENLFILSFKNPILPDLEQYGELPIPPYFERHADETDNVRYQTVFHDPTKLASVAAPTASLHFDDKVLKQLADKGIN
TAYVTLHVGAGTFAPVKTDNLLNHTMHSEYANLPQATADLINQTHQNGNKVIAIGTTVTRVLETAYQKTADEHGHISTWS
GDTDIFIYPGFKFGVIDKLLTNFHLPKSTLLMLVSAFAGKANIEQAYEHAVAQKYRFFSYGDAMLLERNL

Sequences:

>Translated_390_residues
MTQPLSQDQHDSSSNMPTDNAETQGYLSVADYDYDLPDEYIARYPLAERSASRLMYLPAQKENTAVIQDRQFSDLPELLQ
AGDLIVFNDTKVMKARLFGQKDTGGKVEVLVERLTDDTEFTTDESGQSFDNVALCHVRASKAPKLGQRLVLGDGNIECVM
IGRHENLFILSFKNPILPDLEQYGELPIPPYFERHADETDNVRYQTVFHDPTKLASVAAPTASLHFDDKVLKQLADKGIN
TAYVTLHVGAGTFAPVKTDNLLNHTMHSEYANLPQATADLINQTHQNGNKVIAIGTTVTRVLETAYQKTADEHGHISTWS
GDTDIFIYPGFKFGVIDKLLTNFHLPKSTLLMLVSAFAGKANIEQAYEHAVAQKYRFFSYGDAMLLERNL
>Mature_389_residues
TQPLSQDQHDSSSNMPTDNAETQGYLSVADYDYDLPDEYIARYPLAERSASRLMYLPAQKENTAVIQDRQFSDLPELLQA
GDLIVFNDTKVMKARLFGQKDTGGKVEVLVERLTDDTEFTTDESGQSFDNVALCHVRASKAPKLGQRLVLGDGNIECVMI
GRHENLFILSFKNPILPDLEQYGELPIPPYFERHADETDNVRYQTVFHDPTKLASVAAPTASLHFDDKVLKQLADKGINT
AYVTLHVGAGTFAPVKTDNLLNHTMHSEYANLPQATADLINQTHQNGNKVIAIGTTVTRVLETAYQKTADEHGHISTWSG
DTDIFIYPGFKFGVIDKLLTNFHLPKSTLLMLVSAFAGKANIEQAYEHAVAQKYRFFSYGDAMLLERNL

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=366, Percent_Identity=48.3606557377049, Blast_Score=334, Evalue=6e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_PSYWF (A5WGS3)

Other databases:

- EMBL:   CP000713
- RefSeq:   YP_001280814.1
- ProteinModelPortal:   A5WGS3
- SMR:   A5WGS3
- STRING:   A5WGS3
- GeneID:   5206846
- GenomeReviews:   CP000713_GR
- KEGG:   prw:PsycPRwf_1924
- eggNOG:   COG0809
- HOGENOM:   HBG286076
- OMA:   ITRNPAP
- ProtClustDB:   PRK00147
- BioCyc:   PSP56811:PSYCPRWF_1924-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 43537; Mature: 43406

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQPLSQDQHDSSSNMPTDNAETQGYLSVADYDYDLPDEYIARYPLAERSASRLMYLPAQ
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHCCCHHCCCCCEEEECCC
KENTAVIQDRQFSDLPELLQAGDLIVFNDTKVMKARLFGQKDTGGKVEVLVERLTDDTEF
CCCCEEEECCCCCHHHHHHHCCCEEEECCCCEEHHHHCCCCCCCCHHHHHHHHHCCCCCC
TTDESGQSFDNVALCHVRASKAPKLGQRLVLGDGNIECVMIGRHENLFILSFKNPILPDL
CCCCCCCCCCCEEEEEEECCCCCCCCCEEEEECCCEEEEEEECCCCEEEEEECCCCCCCH
EQYGELPIPPYFERHADETDNVRYQTVFHDPTKLASVAAPTASLHFDDKVLKQLADKGIN
HHHCCCCCCCHHHHCCCCCCCEEEEEEECCCHHHHHHCCCCCEECCCHHHHHHHHHCCCC
TAYVTLHVGAGTFAPVKTDNLLNHTMHSEYANLPQATADLINQTHQNGNKVIAIGTTVTR
EEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEECHHHHH
VLETAYQKTADEHGHISTWSGDTDIFIYPGFKFGVIDKLLTNFHLPKSTLLMLVSAFAGK
HHHHHHHHHCCCCCCEEEECCCCEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
ANIEQAYEHAVAQKYRFFSYGDAMLLERNL
CCHHHHHHHHHHHHHHHEECCCEEEEECCC
>Mature Secondary Structure 
TQPLSQDQHDSSSNMPTDNAETQGYLSVADYDYDLPDEYIARYPLAERSASRLMYLPAQ
CCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHCCCHHCCCCCEEEECCC
KENTAVIQDRQFSDLPELLQAGDLIVFNDTKVMKARLFGQKDTGGKVEVLVERLTDDTEF
CCCCEEEECCCCCHHHHHHHCCCEEEECCCCEEHHHHCCCCCCCCHHHHHHHHHCCCCCC
TTDESGQSFDNVALCHVRASKAPKLGQRLVLGDGNIECVMIGRHENLFILSFKNPILPDL
CCCCCCCCCCCEEEEEEECCCCCCCCCEEEEECCCEEEEEEECCCCEEEEEECCCCCCCH
EQYGELPIPPYFERHADETDNVRYQTVFHDPTKLASVAAPTASLHFDDKVLKQLADKGIN
HHHCCCCCCCHHHHCCCCCCCEEEEEEECCCHHHHHHCCCCCEECCCHHHHHHHHHCCCC
TAYVTLHVGAGTFAPVKTDNLLNHTMHSEYANLPQATADLINQTHQNGNKVIAIGTTVTR
EEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEECHHHHH
VLETAYQKTADEHGHISTWSGDTDIFIYPGFKFGVIDKLLTNFHLPKSTLLMLVSAFAGK
HHHHHHHHHCCCCCCEEEECCCCEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
ANIEQAYEHAVAQKYRFFSYGDAMLLERNL
CCHHHHHHHHHHHHHHHEECCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA