Click here to switch to the map view.

The map label for this gene is rep [H]

Identifier: 148653621

GI number: 148653621

Start: 2264597

End: 2266636

Strand: Direct

Name: rep [H]

Synonym: PsycPRwf_1824

Alternate gene names: 148653621

Gene position: 2264597-2266636 (Clockwise)

Preceding gene: 148653619

Following gene: 148653622

Centisome position: 76.02

GC content: 47.4

Gene sequence:

>2040_bases
ATGAGTAAGCTTAACCCCAAACAACAAGAGGCCATGACTTATGTGTCCGGTCCCCTCCTCGTTTTAGCGGGTGCAGGCTC
AGGTAAAACATCGGTTATTACCCGAAAAATTGCTTATCTCATCGAGCAGTGCAATATACCTGCTGAACGCATTACTGCAG
TGACCTTTACCAATAAGGCTGCGCGTGAAATGAAGGCGCGTGTCACCTCACTGATGCCAAAAGAGAAGACCCGCGGTCTA
ACTGTGTCTACGTTCCACCAATTTGGGTTACAGTTTTTGCGTTATGAGCTGCTGCACACGCCTCTCAAAGGCAACTTCTC
TATCATGGATGCCGATGACAGTAAGCGGCTGCTGATGGAGTTGATGATGCGTGATAACTTATCGGGTGCTGAGAGTCGTG
AATTGGTCGGCAAAGCAATTAAGTTTATTTCGGACTGGAAGAATGATCTTATTGAGCCGGATAAAGCAATGGAGACCCTC
GATGATCCTGAGGATATGATTTTTGCCACGCTTTATGCCTTATATGAGCGCAATCTGCGGGCTTATAATGCCGTTGATTT
CGATGACTTGATTGTGTTGCCAGCCAAAATCTTACGTGAAAACCCCCAGCTGCGCGACAAGTGGCAGAACCGTATTAGAT
ATCTGCTGGTGGATGAGTATCAAGATACCAATACTGCTCAGTACGAATTGATTAAATTGATTGTCGGCCCAACTGGCAAG
TTTACTGTGGTGGGTGATGATGACCAATCTATTTATGCTTGGCGCGGCGCCCGTCCCGAGAACATGGCGCTATTAAAAGA
GGATTTCCCTAAGCTTAAAGTGATTAAGCTTGAGCAAAACTACCGCTCGACCAACCGTATTTTGAATGCTGCCAACACCG
TCATTATGAACAACGAGCATTTGTTTGAAAAAGCACTGTGGTCGGATAAAGGGCAAGGCGAGAAAATTCGTATTATTAAC
TGCCGCAACGATGATGATGAATCAGAGCGCGTGGCCAAAGAGATTATTACTCACAAATTGCGCCATGGTAATGAATGGGA
GCAGTATGCCGTCTTGTACCGCAGTAACTTTCAGGCACGTATGTTAGAGGCGCAGCTGCGTCAATTACAGATCCCTTATA
AGCTCTCAGGCGGCCAATCTTTCTTCGCCCGTAGCGAGATTAAAGATGTGATGGGCTATTTGCGCCTTATTTTAAACCCA
GAAGATGACAGTGCCTTTTTGCGCATTGTGAATACGCCCAAACGTGGTCTGGGCCCAGCCACACTAGAGAAGTTGGGACT
GTTCTCTCAAGAGCACAGCATCTCTTTATTGGCCTCTTGTACCCATGGCGGCTTATCACATGTATTGCCCATTAAGGCTT
ATAGTACTTTAAAAGAGTTTGGTGACTTTATCGAGCACTATACCCGTGAGCTGGATCAGCACCCAGATCCGGTGCCTTTG
GTGCGTCAAATGATTGATGAGACCGGTTATATTGACTTTGTGCGCAGCGACTCAAAAACGCCGCATCAGGAAAAAGGGCG
CCTAGACAATATTGAAGTGCTCTACACCAGTATCCAAGCACTTATCAACCGTGCAGAAGATGACGATGACAAAACCATTG
ATGCGGTAATTCGCAAATTGGTATTACTAGACATGCTCGAGCAGCAGCAAGAAGAAGAGAACACCAACAAGGTAAACCTC
ATGACCCTGCATGCTGCCAAAGGGCTAGAGTTTGACTTCGTGTATATTATGGGGCTTGAGGAAGAGCTGCTGCCCCACCG
CAACTCTATCATTGCTGAGACCATTGAGGAGGAGCGCCGCTTGATGTACGTCGGCATCACCCGTGCCCGCCGTGAGCTAA
CCCTAACCTTGGCTGCGCAGCGCCGTGGTGGTGGTCAAATGCGCACTACGACCCAAAGCCGCTTTTTAGAAGAGCTGCCT
GAGGAGCATTTAGACTGGCCCGCCAATGCCAAAAAGAAAAAGGCTGAGCGTGATCCTACTGTGGTGGCCAATGAGTATAT
GTCTAATATTAAGGCGCTGCTAAAAAGAAAAAACCAATAG

Upstream 100 bases:

>100_bases
TCTCAAAGCAGTTGTGATTTCATATTGAACCCCTATAATAACCAGTCTGCCCATTGAGTCGGCAGTCCCCTCTAGTGATA
AGACAATAAAAAGGTGCACC

Downstream 100 bases:

>100_bases
CGGGCAGGTGATGACGTCAGACTGACATCAAATCACAGATTGGCCATGCAAAACCCTAAAAATATTAGTAAAATAAACCA
AAAATGGCAGGATGCACTCC

Product: UvrD/REP helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 679; Mature: 678

Protein sequence:

>679_residues
MSKLNPKQQEAMTYVSGPLLVLAGAGSGKTSVITRKIAYLIEQCNIPAERITAVTFTNKAAREMKARVTSLMPKEKTRGL
TVSTFHQFGLQFLRYELLHTPLKGNFSIMDADDSKRLLMELMMRDNLSGAESRELVGKAIKFISDWKNDLIEPDKAMETL
DDPEDMIFATLYALYERNLRAYNAVDFDDLIVLPAKILRENPQLRDKWQNRIRYLLVDEYQDTNTAQYELIKLIVGPTGK
FTVVGDDDQSIYAWRGARPENMALLKEDFPKLKVIKLEQNYRSTNRILNAANTVIMNNEHLFEKALWSDKGQGEKIRIIN
CRNDDDESERVAKEIITHKLRHGNEWEQYAVLYRSNFQARMLEAQLRQLQIPYKLSGGQSFFARSEIKDVMGYLRLILNP
EDDSAFLRIVNTPKRGLGPATLEKLGLFSQEHSISLLASCTHGGLSHVLPIKAYSTLKEFGDFIEHYTRELDQHPDPVPL
VRQMIDETGYIDFVRSDSKTPHQEKGRLDNIEVLYTSIQALINRAEDDDDKTIDAVIRKLVLLDMLEQQQEEENTNKVNL
MTLHAAKGLEFDFVYIMGLEEELLPHRNSIIAETIEEERRLMYVGITRARRELTLTLAAQRRGGGQMRTTTQSRFLEELP
EEHLDWPANAKKKKAERDPTVVANEYMSNIKALLKRKNQ

Sequences:

>Translated_679_residues
MSKLNPKQQEAMTYVSGPLLVLAGAGSGKTSVITRKIAYLIEQCNIPAERITAVTFTNKAAREMKARVTSLMPKEKTRGL
TVSTFHQFGLQFLRYELLHTPLKGNFSIMDADDSKRLLMELMMRDNLSGAESRELVGKAIKFISDWKNDLIEPDKAMETL
DDPEDMIFATLYALYERNLRAYNAVDFDDLIVLPAKILRENPQLRDKWQNRIRYLLVDEYQDTNTAQYELIKLIVGPTGK
FTVVGDDDQSIYAWRGARPENMALLKEDFPKLKVIKLEQNYRSTNRILNAANTVIMNNEHLFEKALWSDKGQGEKIRIIN
CRNDDDESERVAKEIITHKLRHGNEWEQYAVLYRSNFQARMLEAQLRQLQIPYKLSGGQSFFARSEIKDVMGYLRLILNP
EDDSAFLRIVNTPKRGLGPATLEKLGLFSQEHSISLLASCTHGGLSHVLPIKAYSTLKEFGDFIEHYTRELDQHPDPVPL
VRQMIDETGYIDFVRSDSKTPHQEKGRLDNIEVLYTSIQALINRAEDDDDKTIDAVIRKLVLLDMLEQQQEEENTNKVNL
MTLHAAKGLEFDFVYIMGLEEELLPHRNSIIAETIEEERRLMYVGITRARRELTLTLAAQRRGGGQMRTTTQSRFLEELP
EEHLDWPANAKKKKAERDPTVVANEYMSNIKALLKRKNQ
>Mature_678_residues
SKLNPKQQEAMTYVSGPLLVLAGAGSGKTSVITRKIAYLIEQCNIPAERITAVTFTNKAAREMKARVTSLMPKEKTRGLT
VSTFHQFGLQFLRYELLHTPLKGNFSIMDADDSKRLLMELMMRDNLSGAESRELVGKAIKFISDWKNDLIEPDKAMETLD
DPEDMIFATLYALYERNLRAYNAVDFDDLIVLPAKILRENPQLRDKWQNRIRYLLVDEYQDTNTAQYELIKLIVGPTGKF
TVVGDDDQSIYAWRGARPENMALLKEDFPKLKVIKLEQNYRSTNRILNAANTVIMNNEHLFEKALWSDKGQGEKIRIINC
RNDDDESERVAKEIITHKLRHGNEWEQYAVLYRSNFQARMLEAQLRQLQIPYKLSGGQSFFARSEIKDVMGYLRLILNPE
DDSAFLRIVNTPKRGLGPATLEKLGLFSQEHSISLLASCTHGGLSHVLPIKAYSTLKEFGDFIEHYTRELDQHPDPVPLV
RQMIDETGYIDFVRSDSKTPHQEKGRLDNIEVLYTSIQALINRAEDDDDKTIDAVIRKLVLLDMLEQQQEEENTNKVNLM
TLHAAKGLEFDFVYIMGLEEELLPHRNSIIAETIEEERRLMYVGITRARRELTLTLAAQRRGGGQMRTTTQSRFLEELPE
EHLDWPANAKKKKAERDPTVVANEYMSNIKALLKRKNQ

Specific function: Rep helicase is a single-stranded DNA-dependent ATPase involved in DNA replication; it can initiate unwinding at a nick in the DNA. It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3' to 5' direction [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=678, Percent_Identity=48.0825958702065, Blast_Score=633, Evalue=0.0,
Organism=Escherichia coli, GI2367296, Length=648, Percent_Identity=39.3518518518519, Blast_Score=414, Evalue=1e-116,
Organism=Escherichia coli, GI1787196, Length=361, Percent_Identity=27.7008310249307, Blast_Score=96, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6322369, Length=747, Percent_Identity=26.7737617135207, Blast_Score=189, Evalue=2e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005752
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 78059; Mature: 77928

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLNPKQQEAMTYVSGPLLVLAGAGSGKTSVITRKIAYLIEQCNIPAERITAVTFTNKA
CCCCCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHEEEEEECCHH
AREMKARVTSLMPKEKTRGLTVSTFHQFGLQFLRYELLHTPLKGNFSIMDADDSKRLLME
HHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHH
LMMRDNLSGAESRELVGKAIKFISDWKNDLIEPDKAMETLDDPEDMIFATLYALYERNLR
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHCCC
AYNAVDFDDLIVLPAKILRENPQLRDKWQNRIRYLLVDEYQDTNTAQYELIKLIVGPTGK
CCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHEEEEEECCCCCCCHHHHHHHHEECCCCC
FTVVGDDDQSIYAWRGARPENMALLKEDFPKLKVIKLEQNYRSTNRILNAANTVIMNNEH
EEEEECCCCCEEEECCCCCCCCHHHHHCCCCEEEEEEECHHHHHHHHHHHHCEEEECCHH
LFEKALWSDKGQGEKIRIINCRNDDDESERVAKEIITHKLRHGNEWEQYAVLYRSNFQAR
HHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHH
MLEAQLRQLQIPYKLSGGQSFFARSEIKDVMGYLRLILNPEDDSAFLRIVNTPKRGLGPA
HHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHH
TLEKLGLFSQEHSISLLASCTHGGLSHVLPIKAYSTLKEFGDFIEHYTRELDQHPDPVPL
HHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
VRQMIDETGYIDFVRSDSKTPHQEKGRLDNIEVLYTSIQALINRAEDDDDKTIDAVIRKL
HHHHHHCCCCEEEEECCCCCCCHHCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
VLLDMLEQQQEEENTNKVNLMTLHAAKGLEFDFVYIMGLEEELLPHRNSIIAETIEEERR
HHHHHHHHHHHHCCCCEEEEEEEECCCCCCEEEEEEECCCHHHCCCCHHHHHHHHHHCCE
LMYVGITRARRELTLTLAAQRRGGGQMRTTTQSRFLEELPEEHLDWPANAKKKKAERDPT
EEEEEEHHCCCEEEEEEEEHHCCCCCCCHHHHHHHHHHCCHHHCCCCCCCHHHHHCCCCC
VVANEYMSNIKALLKRKNQ
HHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKLNPKQQEAMTYVSGPLLVLAGAGSGKTSVITRKIAYLIEQCNIPAERITAVTFTNKA
CCCCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHEEEEEECCHH
AREMKARVTSLMPKEKTRGLTVSTFHQFGLQFLRYELLHTPLKGNFSIMDADDSKRLLME
HHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHH
LMMRDNLSGAESRELVGKAIKFISDWKNDLIEPDKAMETLDDPEDMIFATLYALYERNLR
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHCCC
AYNAVDFDDLIVLPAKILRENPQLRDKWQNRIRYLLVDEYQDTNTAQYELIKLIVGPTGK
CCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHEEEEEECCCCCCCHHHHHHHHEECCCCC
FTVVGDDDQSIYAWRGARPENMALLKEDFPKLKVIKLEQNYRSTNRILNAANTVIMNNEH
EEEEECCCCCEEEECCCCCCCCHHHHHCCCCEEEEEEECHHHHHHHHHHHHCEEEECCHH
LFEKALWSDKGQGEKIRIINCRNDDDESERVAKEIITHKLRHGNEWEQYAVLYRSNFQAR
HHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHH
MLEAQLRQLQIPYKLSGGQSFFARSEIKDVMGYLRLILNPEDDSAFLRIVNTPKRGLGPA
HHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHH
TLEKLGLFSQEHSISLLASCTHGGLSHVLPIKAYSTLKEFGDFIEHYTRELDQHPDPVPL
HHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
VRQMIDETGYIDFVRSDSKTPHQEKGRLDNIEVLYTSIQALINRAEDDDDKTIDAVIRKL
HHHHHHCCCCEEEEECCCCCCCHHCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
VLLDMLEQQQEEENTNKVNLMTLHAAKGLEFDFVYIMGLEEELLPHRNSIIAETIEEERR
HHHHHHHHHHHHCCCCEEEEEEEECCCCCCEEEEEEECCCHHHCCCCHHHHHHHHHHCCE
LMYVGITRARRELTLTLAAQRRGGGQMRTTTQSRFLEELPEEHLDWPANAKKKKAERDPT
EEEEEEHHCCCEEEEEEEEHHCCCCCCCHHHHHHHHHHCCHHHCCCCCCCHHHHHCCCCC
VVANEYMSNIKALLKRKNQ
HHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]